高效液相色谱-质谱法分析六种食用蛤中甘油磷脂的分子结构
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- 英文论文题名：Characterization ofglycerophospholipid molecular species in six species of edible clams by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry
- 论文作者：刘中原 ; 周新 ; 张建润 ; 阴法文 ; 周大勇
- 英文论文作者：Liu Zhongyuan ; Zhou Xin ; Zhang Jianrun ; Yin Fawen ; Zhou Dayong ; College of Food Science and Technology ; Dalian Polytechnic University ; National Engineering Research Center of Seafood
- 年：2016
- 作者机构：大连工业大学食品学院国家海洋食品工程技术研究中心;
- 论文关键词：蛤类 ; 甘油磷脂 ; 溶血甘油磷脂 ; 分子结构 ; 高效液相色谱-质谱
- 英文论文关键词：clam ; glycerophospholipid ; lysoglycerophospholipid ; molecular species ; HPLC-ESI-MS/MS
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS254.7
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：112k
- 原文格式：O
- 会议级别：全国
摘要
本文通过高效液相色谱-电喷雾质谱法(HPLC-ESI-MS/MS)对六种食用蛤类中的包括了磷脂酰胆碱(GPCho)、磷脂酰乙醇胺(GPEtn)、磷脂酰丝氨酸(GPSer)、溶血磷脂酰胆碱(LGPCho)和溶血磷脂酰乙醇胺(LGPE)的甘油磷脂分子结构进行分析研究。根据一级质谱图和二级质谱碎片信息,在青蛤(Cyclina sinensis)、中国蛤蜊(Mactra chinensis Philippi)、四角蛤蜊(Mactra veneriformis Reeve)、文蛤(Meretrix meretrix)、菲律宾蛤仔(Ruditapes phliippinarum)、紫石房蛤(Saxidomus purpurata)油脂中共分析鉴定得到甘油磷脂分子结构435、453、468、443、427和444种。蛤类磷脂分子中含多不饱和脂肪酸(PUFA)如20:5(EPA)和22:6(DHA)含量较高。因此,蛤类是获取富含PUFA磷脂的良好来源。根据所含20:5(EPA)和22:6(DHA)磷脂分子相对丰度的不同,可以判定青蛤、四角蛤蜊、中国蛤蜊和紫石房蛤依次是获取GPCho、GPEtn、GPSer/LGPEtn和LGPCho的最优蛤类种属。
The molecular species of glycerophosphocholine(GPCho),glycerophosphoethanolamine(GPEtn),glycerophosphoserine(GPSer),lysoglycerophosphocholine(LGPCho) and lysoglycerophosphoethanolamine(LGPEtn) from six species of edible clams were characterized by using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry for the first time.At least 435,453,468,443,427 and 444 glycerophospholipid(GP) molecular species were characterized,respectively,from Cyclina sinensis,Mactra chinensis Philippi,Mactra veneriformis Reeve,Meretrix meretrix,Ruditapes phliippinarum and Saxidomus purpurata.Most of the predominant GP molecular species in clam contained polyunsaturated fatty acids(PUFA),mainly 20:5 and 22:6,indicating that clam is a potential resource of GP enriched PUFA.According to the amount of the major molecular species containing 20:5 and 22:6,Cyclina sinensis was the best fit species for GPCho,Mactra veneriformis Reeve was the best fit species for GPEtn,Mactra chinensis Philippi was the best fit species for GPSer and LGPEtn,and Saxidomus purpurata was the best fit species for LGPCho.
The molecular species of glycerophosphocholine(GPCho),glycerophosphoethanolamine(GPEtn),glycerophosphoserine(GPSer),lysoglycerophosphocholine(LGPCho) and lysoglycerophosphoethanolamine(LGPEtn) from six species of edible clams were characterized by using high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry for the first time.At least 435,453,468,443,427 and 444 glycerophospholipid(GP) molecular species were characterized,respectively,from Cyclina sinensis,Mactra chinensis Philippi,Mactra veneriformis Reeve,Meretrix meretrix,Ruditapes phliippinarum and Saxidomus purpurata.Most of the predominant GP molecular species in clam contained polyunsaturated fatty acids(PUFA),mainly 20:5 and 22:6,indicating that clam is a potential resource of GP enriched PUFA.According to the amount of the major molecular species containing 20:5 and 22:6,Cyclina sinensis was the best fit species for GPCho,Mactra veneriformis Reeve was the best fit species for GPEtn,Mactra chinensis Philippi was the best fit species for GPSer and LGPEtn,and Saxidomus purpurata was the best fit species for LGPCho.
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