LC-MS-MS法测定动物组织中苯乙醇胺A残留的方法研究
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- 英文论文题名：The Study on LC-MS-MS method for the determination of Phenylethanolamine A residue in animal tissue
- 论文作者：李树纲 ; 才让卓玛 ; 李树星 ; 杨红岩
- 英文论文作者：LI Shu-gang ; ZHUO MA Cai-rang ; YANG Hong-yan ; Gansu Institute of Veterinary Drug and Feed Inspection ; Gansu Provincial Maternity and Child-care Hospital
- 年：2016
- 作者机构：甘肃省兽药饲料监察所;甘肃省妇幼保健院;
- 论文关键词：苯乙醇胺A ; 动物组织 ; 残留 ; LC-MS-MS
- 英文论文关键词：phenylethanolamine A ; animal tissue ; residue ; LC-MS-MS
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.84;O657.63
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：5
- 文件大小：856k
- 原文格式：O
- 会议级别：全国
摘要
建立了LC-MS-MS法测定动物组织中苯乙醇胺A残留的方法。采用了高效液相色谱-串联质谱法,流动相为乙腈-0.2%甲酸水溶液(65:35),流速为0.3ml/min,柱温为30℃,离子源为ESI+。苯乙醇胺A在2.0~100.0ng/mL的范围内呈现良好的线性,r为0.9996。方法的检测限为0.2μg/kg,定量限为0.5μg/kg。苯乙醇胺A在0.2μg/kg~10μg/kg的添加范围内平均回收率为75.2%~108.2%,批内、批间RSD均小于20%。本方法简便快捷、灵敏度高、定性准确,能满足动物性食品安全检测有关法规的要求。
LC-MS-MS method was established for the determination of phenylethanolamine A residue in animal tissue.Using high performance liquid chromatography tandem mass spectrometry,the mobile phase was acetonitrile-0.2%formic acid(65:35),the flow rate was 0.3ml/min,column temperature was 30℃,ion source was ESI+.Phenylethanolamine A showed a good linear in the range of2~100.0ng/mL,r was 0.9996.The limit of detection is 0.2μg/kg,the limit of quantitation was 0.5μg/kg.At 0.2μg/kg to 10μg/kg add context,the average recovery rate of phenylethanolamine A was 75.2%to 108.2%,the RSD of batch and between group were less than 20%.This method is simple,rapid,high sensitivity,accurate,and can meet the animal food safety detection of relevant regulations.
LC-MS-MS method was established for the determination of phenylethanolamine A residue in animal tissue.Using high performance liquid chromatography tandem mass spectrometry,the mobile phase was acetonitrile-0.2%formic acid(65:35),the flow rate was 0.3ml/min,column temperature was 30℃,ion source was ESI+.Phenylethanolamine A showed a good linear in the range of2~100.0ng/mL,r was 0.9996.The limit of detection is 0.2μg/kg,the limit of quantitation was 0.5μg/kg.At 0.2μg/kg to 10μg/kg add context,the average recovery rate of phenylethanolamine A was 75.2%to 108.2%,the RSD of batch and between group were less than 20%.This method is simple,rapid,high sensitivity,accurate,and can meet the animal food safety detection of relevant regulations.
引文
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[2]刘彩云,周围,毕阳,等.液质联用测定饲料中的β-兴奋剂[J].安徽农业科学,2007,35(17):5178~5179.
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