A self-assembly/disassembly two-photo ratiometric fluorogenic probe for bacteria imaging

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英文篇名：A self-assembly/disassembly two-photo ratiometric fluorogenic probe for bacteria imaging 作者：Shuangshuang ; Long ; Qinglong ; Qiao ; Lu ; Miao ; Zhaochao ; Xu 英文作者：Shuangshuang Long;Qinglong Qiao;Lu Miao;Zhaochao Xu;CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences;University of Chinese Academy of Sciences; 英文关键词：Fluorogenic probe;;Bacteria;;Self-assembly;;Imidazolium;;Pyrene 中文刊名：FXKB 英文刊名：中国化学快报(英文版) 机构：CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences;University of Chinese Academy of Sciences; 出版日期：2019-03-15 出版单位：Chinese Chemical Letters 年：2019 期：v.30 基金：financially supported by the National Natural Science Foundation of China(Nos. 21878286, 21502189);; DICP (Nos. DMT0201603, TMSR201601) 语种：英文; 页：FXKB201903010 页数：4 CN：03 ISSN：11-2710/O6 分类号：61-64

摘要

Fluorescence imaging has facilitated fluorescent probes to analyze the subcellular localization and dynamics of biological targets. In this paper, we reported a fluorogenic probe for bacteria imaging. The probe was an imidazolium-derived pyrene compound, which self-assembled to form nano-particles and the pyrene fluorescence was quenched by the aggregation effects. When the self-assembly nanoparticles interacted with anionic bacteria surfaces, synergistic effects of electrostatic interaction and hydrophobic force caused competing binding between bacteria surfaces and imidazoliums. This binding resulted in the disassembly of the aggregates to give fluorescence turn-on signal. Meanwhile, the probe bound bacteria surfaces and displayed both pyrene-excimer and pyrene-monomer fluorescence, which gave ratiometric signal. Then, fluorescent labeling by the probe enabled the two-photo ratiometric imaging of bacteria.
        Fluorescence imaging has facilitated fluorescent probes to analyze the subcellular localization and dynamics of biological targets. In this paper, we reported a fluorogenic probe for bacteria imaging. The probe was an imidazolium-derived pyrene compound, which self-assembled to form nano-particles and the pyrene fluorescence was quenched by the aggregation effects. When the self-assembly nanoparticles interacted with anionic bacteria surfaces, synergistic effects of electrostatic interaction and hydrophobic force caused competing binding between bacteria surfaces and imidazoliums. This binding resulted in the disassembly of the aggregates to give fluorescence turn-on signal. Meanwhile, the probe bound bacteria surfaces and displayed both pyrene-excimer and pyrene-monomer fluorescence, which gave ratiometric signal. Then, fluorescent labeling by the probe enabled the two-photo ratiometric imaging of bacteria.

引文

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