南美白对虾霍乱弧菌的分离与鉴定研究

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英文篇名：Isolation and identification of Vibrio cholera from Penaeus vannamei 作者：陈嘉俊 ; 朱焯安 ; 汪健 ; 廖国威 ; 梁日深 ; 林蠡 ; 吴灶和 英文作者：CHEN Jiajun;ZHU Zhuoan;WANG Jian;LIAO Guowei;LIANG Rishen;LIN Li;WU Zaohe;College of animals science and technology, Zhongkai University of Agriculture and Engineering;Guangdong Provincial Key Laboratory of Waterfowl Healthy Culture;Guangdong Provincial Water Environment and Aquatic Products Security Engineering Techrology Research Center;Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding; 关键词：霍乱弧菌(Vibrio ; cholera) ; 鉴定 ; 药敏试验 ; 最小抑菌浓度 英文关键词：Vibrio cholera;;identification;;drug sensitive test;;minimum inhibitory concentration(MIC) 中文刊名：ZNJX 英文刊名：Journal of Zhongkai University of Agriculture and Engineering 机构：仲恺农业工程学院动物科技学院;广东省水禽健康养殖重点实验室;广东省水环境与水产品安全工程技术研究中心;广州市水产病害与水禽养殖重点实验室; 出版日期：2019-05-17 16:21 出版单位：仲恺农业工程学院学报 年：2019 期：v.32 基金：广东省自然科学基金(2016A030310236);; 广东省大学生创新创业训练计划(201811347083);; 广东省科技计划(2017A040403060);; 仲恺农业工程学院创新基金(2018A12)资助项目 语种：中文; 页：ZNJX201902011 页数：5 CN：02 ISSN：44-1660/S 分类号：53-57

摘要

目前南美白对虾(Penaeus vannamei)人工养殖中,病害多发,本研究从一养殖场中采集发病病虾,从患病对虾肝胰腺中分离病原菌.对分离菌株进行形态观察,生理生化测定并结合16S rDNA序列分析进行鉴定,结果表明,该分离菌为霍乱弧菌(Vibrio cholera),回归感染证明其为致病菌,可导致南美白对虾死亡.通过纸片琼脂扩散法对32种抗生素进行药物敏感试验,病原菌对氟苯尼考、氯霉素、利福平、诺氟沙星等10种抗生素敏感,对头孢呋辛、头孢曲松等7种药物中度敏感,对羧苄西林、青霉素等15种药物具有抗性.通过二倍稀释法测定筛选5种常用抗生素最小抑菌浓度,结果表明诺氟沙星抑菌效果最强,最小抑菌浓度最小<0.244 1μg/mL.
        Recently, diseases seriously outbreak in Penaeus vannamei of artificial breeding. In this study, the infected Penaeus vannamei were collected from one aquaculture farm and the pathogen was isolated from hepatopancreas. The isolated pathogen was identified via morphological observation, physiological and biochemical characters and 16 S rDNA sequence analysis. The results showed that, the isolated bacterium was identified as Vibrio cholera. The regression experiment also demonstrated it was the pathogen which caused the death of the shrimp. The drug sensitive test of 32 antibiotics was detected using K-B way, the results showed that V. cholerae was highly sensitive to 10 antibiotics including florfenicol, chloramphenicol, rifampicin, norfloxacin, etc; intermediate sensitive to 7 antibiotics including cefuroxime, ceftriaxone, etc; resistant to 15 antibiotics including carboxy ampicillin, penicillin, etc. The minimum inhibitory concentration(MIC) of 5 selected antibiotics was also detected using tube double dilution method, norfloxacin showed the best inhibiting effect and the value was less than 0.244 1 μg/mL.

引文

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