副溶血弧菌特异性卵黄抗体(AHPND-VpIgY)对凡纳滨对虾幼体被动免疫和育苗成活率的影响
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摘要
将抗高致病性副溶血弧菌(Vibrioparahaemolyticus)特异性卵黄抗体(AHPND-VpIgY)应用于育苗生产,通过测定氨氮、亚硝酸盐、细菌总数、弧菌总数、相对保护率和育苗成活率,评价了AHPND-VpIgY在育苗期对凡纳滨对虾(Litopenaeusvannamei)幼体的保护效果。结果表明,0.1g/m3和0.2g/m3AHPND-VpIgY组的相对保护率为35.7%和57.2%,两者均显著高于非特异性卵黄抗体组(P<0.05);两组的育苗成活率为50.8%和57.2%,比对照组分别提高19.1%和25.6%。各实验组氨氮、亚硝酸盐、细菌总数及弧菌总数与对照组无显著差异,对水质无影响。综上,特异性的AHPND-VpIgY能在不改变水质的前提下,有效提高凡纳滨对虾幼体的存活率、相对保护率和育苗成活率,从而提高凡纳滨对虾苗种产量和质量。
We applied the specific egg yolk antibody of highly pathogenic Vibrio parahaemolyticus(AHPND-VpIgY)for seedling breeding of Litopenaeus vannamei. The protective effect with the antibody was evaluated by measuring water quality, total bacteria, total Vibrio count, relative protection rate, and seedling survival rate. The results show that the relative protection rate of the 0.1 g/m3 and 0.2 g/m3 AHPND-VpIgY groups was 35.7% and 57.2%, respectively, which is higher than that of the control(no AHPND-VpIgY)(P<0.05). The larva survival rate of the 0.1 g/m3 and 0.2 g/m3 AHPND-VpIgY groups was 50.8% and 57.2%, which was 19.1% and 25.6% higher than that of the control group,respectively. Meanwhile, no significant difference in ammonia nitrogen, nitrite, total bacteria, and total Vibrio count were found between the experiment groups and control group. Therefore, application of AHPND-VpIgY could increase the survival rate of the larva, the relative protection rate, and the survival rate of seedling, and enhance the yield and quality of L. vannamei seedlings while the water quality remained unchanged.
We applied the specific egg yolk antibody of highly pathogenic Vibrio parahaemolyticus(AHPND-VpIgY)for seedling breeding of Litopenaeus vannamei. The protective effect with the antibody was evaluated by measuring water quality, total bacteria, total Vibrio count, relative protection rate, and seedling survival rate. The results show that the relative protection rate of the 0.1 g/m3 and 0.2 g/m3 AHPND-VpIgY groups was 35.7% and 57.2%, respectively, which is higher than that of the control(no AHPND-VpIgY)(P<0.05). The larva survival rate of the 0.1 g/m3 and 0.2 g/m3 AHPND-VpIgY groups was 50.8% and 57.2%, which was 19.1% and 25.6% higher than that of the control group,respectively. Meanwhile, no significant difference in ammonia nitrogen, nitrite, total bacteria, and total Vibrio count were found between the experiment groups and control group. Therefore, application of AHPND-VpIgY could increase the survival rate of the larva, the relative protection rate, and the survival rate of seedling, and enhance the yield and quality of L. vannamei seedlings while the water quality remained unchanged.
引文
王斌,刘永波,刘双凤等,2006.鸡抗迟缓爱德华氏菌卵黄抗体的应用研究.大连海洋大学学报,21(3):203-206
朱香萍,张再生,刘宗柱,2008.对虾鳗弧菌卵黄抗体(IgY)的制备及其对人工感染的保护研究.海洋科学,32(2):25-28
庄政,2008.嗜水气单胞菌亚单位成分卵黄抗体在鳗鲡养殖中应用.福州:福建农林大学硕士学位论文
陈强,周建武,饶平凡等,2001.卵黄抗体在防治中华鳖气单胞菌病中的应用.福州大学学报(自然科学版),29(5):115-118,126
唐小千,徐洪森,战文斌,2016.对虾急性肝胰腺坏死综合症研究进展.海洋湖沼通报,(2):90-93
Arasteh N,Aminirissehei A H,Yousif A N et al,2004.Passive immunization of rainbow trout(Oncorhynchus mykiss)with chicken egg yolk immunoglobulins(IgY).Aquaculture,231(1-4):23-36
Bailey J H,Moss M S,1992.Penaeid taxonomy,biology and zoogeography.In:Fast A W,Lester L J eds.Marine Shrimp Culture.The Netherlands:Elsevier Science Publishers,9-73
Eto S F,Fernandes D C,Moraes A C et al,2018.Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia(Oreochromis niloticus).Fish&Shellfish Immunology,76:153-160
Gao X J,Chen N,Zhang Y et al,2017.Non-O1 Vibrio cholerae pathogen from Cyprinus carpio and control with anti-non-O1 V.cholerae egg yolk powder(IgY).Aquaculture,479:69-74
Gutierrez M,Miyazaki A,Hatta T et al,1993.Protective properties of egg yolk IgY containing anti-Edwardsiella tarda antibody against paracolo disease in the Japanese eel,Anguilla japonica Temminck&Schlegel.Journal of Fish Diseases,16(2):113-122
Hatta H,Tsuda K,Akachi S et al,1993a.Productivity and some properties of egg yolk antibody(IgY)against human rotavirus compared with rabbit IgG.Bioscience Biotechnology and Biochemistry,53(3):450-454
Hatta H,Tsuda K,Akachi S et al,1993b.Oral passive immunization effect of anti-human rotavirus IgY and its behavior against proteolytic enzymes.Bioscience Biotechnology and Biochemistry,57(7):1077-1081
Hatta H,Mabe K,Kim M et al,1994.Prevention of fish disease using egg yolk antibody.In:Sim J S,Nakai S eds.Egg Uses and Processing Technologies.Wallingford:CABInternational
Kim D K,Jang I K,Seo H C et al,2004.Shrimp protected from WSSV disease by treatment with egg yolk antibodies(IgY)against a truncated fusion protein derived from WSSV.Aquaculture,237(1-4):21-30
Kondo H,Van P T,Dang L T et al,2015.Draft genome sequence of non-Vibrio parahaemolyticus acute hepatopancreatic necrosis disease strain KC13.17.5.isolated from diseased shrimp in Vietnam.Genome Announcements,3(5):e00978-15
Kumaran T,Thirumalaikumar E,Lelin C et al,2018.Physicochemical properties of anti Vibrio harveyi egg yolk antibody(IgY)and its immunological influence in Indian white shrimp Fenneropenaeus indicus.Fish&Shellfish Immunology,74:349-362
Lee E N,Sunwoo H H,Menninen K et al,2002.In vitro studies of chicken egg yolk antibody(IgY)against Salmonella enteritidis and Salmonella typhimurium.Poultry Science,81(5):632-641
Li C H,Lu X J,Li D F et al,2014.Passive protective effect of chicken egg yolk immunoglobulins against experimental Vibrio anguillarum infection in ayu(Plecoglossus altivelis).Fish&Shellfish Immunology,37(1):108-114
Qin Z D,Babu V S,Li N Q et al,2018.Protective effects of chicken egg yolk immunoglobulins(IgY)against experimental Aeromonas hydrophila infection in blunt snout bream(Megalobrama amblycephala).Fish&Shellfish Immunology,78:26-34
Shin J H,Nam S W,Kim J T et al,2013.Identification of immunodominant Helicobacter pylori proteins with reactivity to H.pylori-specific egg-yolk immunoglobulin.Journal of Medical Microbiology,52(3):217-222
Soto-Rodriguez S A,Gomez-Gil B,Lozano-Olvera R et al,2015.Field and experimental evidence of Vibrio parahaemolyticus as the causative agent of acute hepatopancreatic necrosis disease of cultured shrimp(Litopenaeus vannamei)in northwestern Mexico.Applied and Environmental Microbiology,81(5):1689-1699
Tran L,Nunan L,Redman R M et al,2013.Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp.Diseases of Aquatic Organisms,105(1):45-55
Tran L H,Fitzsimmons K,Lightner D V,2014.Ecological approaches in controlling the acute hepatopancreatic necrosis disease.In:The Ninth Symposium on Diseases in Asian Aquaculture.Vietnam:Ho Chi Minh City
Winkelbach A,Wuertz S,Schade R et al,2017.Effects of oral passive immunization against somatostatin-14 on growth performance,body composition and IgY delivery in rainbow trout(Oncorhynchus mykiss)and common carp(Cyprinus carpio).Aquaculture Nutrition,23(2):387-396
Xu L,Xu Y P,He L Y et al,2019.Immunomodulatory effects of chicken egg yolk antibodies(IgY)against experimental Shewanella marisflavi AP629 infections in sea cucumbers(Apostichopus japonicus).Fish&Shellfish Immunology,84:108-119
Yi L Z,Qin Z D,Lin H Z et al,2018.Features of chicken egg yolk immunoglobulin(IgY)against the infection of red-spotted grouper nervous necrosis virus.Fish&Shellfish Immunology,80:534-539
朱香萍,张再生,刘宗柱,2008.对虾鳗弧菌卵黄抗体(IgY)的制备及其对人工感染的保护研究.海洋科学,32(2):25-28
庄政,2008.嗜水气单胞菌亚单位成分卵黄抗体在鳗鲡养殖中应用.福州:福建农林大学硕士学位论文
陈强,周建武,饶平凡等,2001.卵黄抗体在防治中华鳖气单胞菌病中的应用.福州大学学报(自然科学版),29(5):115-118,126
唐小千,徐洪森,战文斌,2016.对虾急性肝胰腺坏死综合症研究进展.海洋湖沼通报,(2):90-93
Arasteh N,Aminirissehei A H,Yousif A N et al,2004.Passive immunization of rainbow trout(Oncorhynchus mykiss)with chicken egg yolk immunoglobulins(IgY).Aquaculture,231(1-4):23-36
Bailey J H,Moss M S,1992.Penaeid taxonomy,biology and zoogeography.In:Fast A W,Lester L J eds.Marine Shrimp Culture.The Netherlands:Elsevier Science Publishers,9-73
Eto S F,Fernandes D C,Moraes A C et al,2018.Validation of IgY for the diagnosis of Streptococcus agalactiae-caused endocarditis and bacterial meningitis in Nile tilapia(Oreochromis niloticus).Fish&Shellfish Immunology,76:153-160
Gao X J,Chen N,Zhang Y et al,2017.Non-O1 Vibrio cholerae pathogen from Cyprinus carpio and control with anti-non-O1 V.cholerae egg yolk powder(IgY).Aquaculture,479:69-74
Gutierrez M,Miyazaki A,Hatta T et al,1993.Protective properties of egg yolk IgY containing anti-Edwardsiella tarda antibody against paracolo disease in the Japanese eel,Anguilla japonica Temminck&Schlegel.Journal of Fish Diseases,16(2):113-122
Hatta H,Tsuda K,Akachi S et al,1993a.Productivity and some properties of egg yolk antibody(IgY)against human rotavirus compared with rabbit IgG.Bioscience Biotechnology and Biochemistry,53(3):450-454
Hatta H,Tsuda K,Akachi S et al,1993b.Oral passive immunization effect of anti-human rotavirus IgY and its behavior against proteolytic enzymes.Bioscience Biotechnology and Biochemistry,57(7):1077-1081
Hatta H,Mabe K,Kim M et al,1994.Prevention of fish disease using egg yolk antibody.In:Sim J S,Nakai S eds.Egg Uses and Processing Technologies.Wallingford:CABInternational
Kim D K,Jang I K,Seo H C et al,2004.Shrimp protected from WSSV disease by treatment with egg yolk antibodies(IgY)against a truncated fusion protein derived from WSSV.Aquaculture,237(1-4):21-30
Kondo H,Van P T,Dang L T et al,2015.Draft genome sequence of non-Vibrio parahaemolyticus acute hepatopancreatic necrosis disease strain KC13.17.5.isolated from diseased shrimp in Vietnam.Genome Announcements,3(5):e00978-15
Kumaran T,Thirumalaikumar E,Lelin C et al,2018.Physicochemical properties of anti Vibrio harveyi egg yolk antibody(IgY)and its immunological influence in Indian white shrimp Fenneropenaeus indicus.Fish&Shellfish Immunology,74:349-362
Lee E N,Sunwoo H H,Menninen K et al,2002.In vitro studies of chicken egg yolk antibody(IgY)against Salmonella enteritidis and Salmonella typhimurium.Poultry Science,81(5):632-641
Li C H,Lu X J,Li D F et al,2014.Passive protective effect of chicken egg yolk immunoglobulins against experimental Vibrio anguillarum infection in ayu(Plecoglossus altivelis).Fish&Shellfish Immunology,37(1):108-114
Qin Z D,Babu V S,Li N Q et al,2018.Protective effects of chicken egg yolk immunoglobulins(IgY)against experimental Aeromonas hydrophila infection in blunt snout bream(Megalobrama amblycephala).Fish&Shellfish Immunology,78:26-34
Shin J H,Nam S W,Kim J T et al,2013.Identification of immunodominant Helicobacter pylori proteins with reactivity to H.pylori-specific egg-yolk immunoglobulin.Journal of Medical Microbiology,52(3):217-222
Soto-Rodriguez S A,Gomez-Gil B,Lozano-Olvera R et al,2015.Field and experimental evidence of Vibrio parahaemolyticus as the causative agent of acute hepatopancreatic necrosis disease of cultured shrimp(Litopenaeus vannamei)in northwestern Mexico.Applied and Environmental Microbiology,81(5):1689-1699
Tran L,Nunan L,Redman R M et al,2013.Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp.Diseases of Aquatic Organisms,105(1):45-55
Tran L H,Fitzsimmons K,Lightner D V,2014.Ecological approaches in controlling the acute hepatopancreatic necrosis disease.In:The Ninth Symposium on Diseases in Asian Aquaculture.Vietnam:Ho Chi Minh City
Winkelbach A,Wuertz S,Schade R et al,2017.Effects of oral passive immunization against somatostatin-14 on growth performance,body composition and IgY delivery in rainbow trout(Oncorhynchus mykiss)and common carp(Cyprinus carpio).Aquaculture Nutrition,23(2):387-396
Xu L,Xu Y P,He L Y et al,2019.Immunomodulatory effects of chicken egg yolk antibodies(IgY)against experimental Shewanella marisflavi AP629 infections in sea cucumbers(Apostichopus japonicus).Fish&Shellfish Immunology,84:108-119
Yi L Z,Qin Z D,Lin H Z et al,2018.Features of chicken egg yolk immunoglobulin(IgY)against the infection of red-spotted grouper nervous necrosis virus.Fish&Shellfish Immunology,80:534-539
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