副溶血性弧菌分泌系统在致病力中作用的研究进展
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摘要
致病菌借助分泌系统将特异蛋白直接注入宿主细胞内,破坏宿主细胞内的多种信号通路,是导致细菌定殖和感染的有效途径。作为一种重要的食源性致病菌,副溶血性弧菌(Vibrio parahaemolyticus)的Ⅲ型分泌系统(Type Ⅲ secretion system,T3SS)和Ⅵ型分泌系统(Type Ⅵ secretion system,T6SS)是其对宿主细胞产生致病性的重要因素。本文综述了副溶血性弧菌T3SS和T6SS效应物在致病力中的具体作用,以及相关调控机理,为进一步了解由副溶血性弧菌导致的病症,研究其致病机理以及寻找致病性靶标提供参考。
Specialized substrates are injected into a host cell through secretion system, to destroy various signal pathways, which is an effective way for bacterial colonization and infection. Vibrio parahaemolyticus being an important foodborne pathogen, its Type Ⅲ Secretion System(T3SS) and Type VI Secretion System(T6SS) are important virulence factors to the hosts. In this review, we summarize the specific roles of T3SS and T6SS effectors in pathogenicity and the related regulatory mechanisms. This paper provides a reference to further understand the diseases caused by Vibrio parahaemolyticus, to investigate its pathogenesis and find the pathogenic targets.
Specialized substrates are injected into a host cell through secretion system, to destroy various signal pathways, which is an effective way for bacterial colonization and infection. Vibrio parahaemolyticus being an important foodborne pathogen, its Type Ⅲ Secretion System(T3SS) and Type VI Secretion System(T6SS) are important virulence factors to the hosts. In this review, we summarize the specific roles of T3SS and T6SS effectors in pathogenicity and the related regulatory mechanisms. This paper provides a reference to further understand the diseases caused by Vibrio parahaemolyticus, to investigate its pathogenesis and find the pathogenic targets.
引文
[1]Mao XD,Hu JF,Liu XM.Study on incidence of foodborne disease caused by Vibrio parahaemolyticus by literature review method.Chinese Journal of Disease Control&Prevention,2013,17(3):265-267.(in Chinese)毛雪丹,胡俊峰,刘秀梅.用文献综述法估计我国食源性副溶血性弧菌病发病率.中华疾病控制杂志,2013,17(3):265-267.
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[33]Kodama T,Hiyoshi H,Gotoh K,Akeda Y,Matsuda S,Park KS,Cantarelli VV,Iida T,Honda T.Identification of two translocon proteins of Vibrio parahaemolyticus type IIIsecretion system 2.Infection&Immunity,2008,76(9):4282-4289.
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[36]Osei-Adjei G,Gao H,Zhang Y,Zhang LY,Yang WH,Yang HY,Yin Z,Huang XX,Zhang YQ,Zhou DS.Regulatory actions of ToxR and CalR on their own genes and type IIIsecretion system 1 in Vibrio parahaemolyticus.Oncotarget,2017,8:65809-65822.
[37]Li P,Rivera-Cancel G,Kinch LN,Salomon D,Tomchick DR,Grishin NV,Orth K.Bile salt receptor complex activates a pathogenic type III secretion system.eLife,2016,5:e15718.
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[40]Pukatzki S,Ma A T,Sturtevant D,Krastins B,Sarracino D,Nelson WC,Heidelberg JF,Mekalanos JJ.Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system.Proceedings of the National Academy of Sciences of the United States of America,2006,103(5):1528-1533.
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[2]Liu HQ,Liu BX,Lv LQ,Pan YJ,Xie J,Zhao Y.Risk analysis of Vibrio parahaemolyticus in edible raw salmon marketed in Shanghai.Food Science,2015,36(24):195-199.(in Chinese)刘海泉,刘冰宣,吕利群,潘迎捷,谢晶,赵勇.上海市生食三文鱼中副溶血性弧菌污染的风险分析.食品科学,2015,36(24):195-199.
[3]Wang JJ,Sun WS,Jin MT,Liu H,Zhang WJ,Sun XH,Pan YJ,Zhao Y.Fate of Vibrio parahaemolyticus on shrimp after acidic electrolyzed water treatment.International Journal of Food Microbiology,2014,179:50-56.
[4]Ceccarelli D,Hasan NA,Huq A,Colwell RR.Distribution and dynamics of epidemic and pandemic Vibrio parahaemolyticus virulence factors.Frontiers in Cellular&Infection Microbiology,2013,3:97.
[5]Park KS,Ono T,Rokuda M,Jang MH,Iida T,Honda T.Cytotoxicity and enterotoxicity of the thermostable direct hemolysin-deletion mutants of Vibrio parahaemolyticus.Microbiology&Immunology,2004,48(4):313-318.
[6]de Nisco NJ,Kanchwala M,Li P,Fernandez J,Xing C,Orth K.The cytotoxic type 3 secretion system 1 of Vibrio rewires host gene expression to subvert cell death and activate cell survival pathways.Science Signaling,2017,10(479):eaal4501.
[7]Ono T,Park KS,Ueta M,Iida T,Honda T.Identification of proteins secreted via Vibrio parahaemolyticus type IIIsecretion system 1.Infection and Immunity,2006,74(2):1032-1042.
[8]Sreelatha A,Bennett TL,Zheng H,Jiang QX,Orth K,Starai VJ.Vibrio effector protein,VopQ,forms a lysosomal gated channel that disrupts host ion homeostasis and autophagic flux.Proceedings of the National Academy of Sciences of the United States of America,2013,110(28):11559-11564.
[9]Burdette DL,Seemann J,Orth K.Vibrio VopQ induces PI3-kinase-independent autophagy and antagonizes phagocytosis.Molecular Microbiology,2009,73(4):639-649.
[10]Salomon D,Guo YR,Kinch LN,Grishin NV,Gardner KH,Orth K.Effectors of animal and plant pathogens use a common domain to bind host phosphoinositides.Nature Communications,2013,4:2973.
[11]Bhattacharjee RN,Park KS,Kumagai Y,Okada K,Yamamoto M,Uematsu S,Matsui K,Kumar H,Kawai T,Iida,T,Honda T,Takeuchi O,Akira S.VP1686,a Vibrio type III secretion protein,induces toll-like receptor-independent apoptosis in macrophage through NF-κB inhibition.Journal of Biological Chemistry,2006,281(48):36897-36904.
[12]Creech C,Kanauja M,Causey CP.Synthesis and evaluation of2-ethynyl-adenosine-5′-triphosphate as a chemical reporter for protein AMPylation.Organic&Biomolecular Chemistry,2015,13(31):8550-8555.
[13]Yarbrough ML,Li Y,Kinch LN,Grishin NV,Ball HL,Orth K.AMPylation of Rho GTPases by Vibrio VopS disrupts effector binding and downstream signaling.Science,2009,323(5911):269-272.
[14]Broberg CA,Zhang L,Gonzalez H,Laskowski-Arce MA,Orth K.A Vibrio effector protein is an inositol phosphatase and disrupts host cell membrane integrity.Science,2010,329(5999):1660-1662.
[15]Zhou XH,Konkel ME,Call DR.Vp1659 is a Vibrio parahaemolyticus type III secretion system 1 protein that contributes to translocation of effector proteins needed to induce cytolysis,autophagy,and disruption of actin structure in HeLa cells.Journal of Bacteriology,2010,192(13):3491-3502.
[16]Shimohata T,Mawatari K,Iba H,Hamano M,Negoro S,Asada S,Aihara M,Hirata A,Su ZH,Takahashi A.VopB1 and VopD1 are essential for translocation of type III secretion system 1 effectors of Vibrio parahaemolyticus.Canadian Journal of Microbiology,2012,58(8):1002-1007.
[17]Hiyoshi H,Kodama T,Saito K,Gotoh K,Matsuda S,Akeda Y,Honda T,Iida T.VopV,an F-actin-binding type III secretion effector,is required for Vibrio parahaemolyticus-induced enterotoxicity.Cell Host&Microbe,2011,10(4):401-409.
[18]Yu BK,Cheng HC,Brautigam CA,Tomchick DR,Rosen MK.Mechanism of actin filament nucleation by the bacterial effector VopL.Nature Structural&Molecular Biology,2011,18(9):1068-1074.
[19]de Souza Santos M,Salomon D,Orth K.T3SS effector VopLinhibits the host ROS response,promoting the intracellular survival of Vibrio parahaemolyticus.PLoS Pathogens,2017,13(6):e1006438.
[20]Ray K,Marteyn B,Sansonetti PJ,Tang CM.Life on the inside:the intracellular lifestyle of cytosolic bacteria.Nature Reviews Microbiology,2009,7(5):333-340.
[21]de Souza Santos M,Orth K.Intracellular Vibrio parahaemolyticus escapes the vacuole and establishes a replicative niche in the cytosol of epithelial cells.mBio,2014,5(5):e01506-14.
[22]Okada R,Zhou XH,Hiyoshi H,Matsuda S,Chen X,Akeda Y,Kashimoto T,Davis BM,Iida T,Waldor MK,Kodama T.The Vibrio parahaemolyticus effector VopC mediates Cdc42-dependent invasion of cultured cells but is not required for pathogenicity in an animal model of infection.Cellular Microbiology,2014,16(6):938-947.
[23]Hiyoshi H,Okada R,Matsuda S,Gotoh K,Akeda Y,Iida T,Kodama T.Interaction between the type III effector VopO and GEF-H1 activates the RhoA-ROCK pathway.PLoSPathogens,2015,11(3):e1004694.
[24]Liverman ADB,Cheng HC,Trosky JE,Leung DW,Yarbrough ML,Burdette DL,Rosen MK,Orth K.Arp2/3-independent assembly of actin by Vibrio type III effector VopL.Proceedings of the National Academy of Sciences of the United States of America,2007,104(43):17117-17122.
[25]Trosky JE,Li Y,Mukherjee S,Keitany G,Ball H,Orth K.VopA inhibits ATP binding by acetylating the catalytic loop of MAPK kinases.Journal of Biological Chemistry,2007,282(47):34299-34305.
[26]Caburlotto G,LleòMM,Hilton T,Huq A,Colwell RR,Kaper JB.Effect on human cells of environmental Vibrio parahaemolyticus strains carrying type III secretion system 2.Infection&Immunity,2010,78(7):3280-3287.
[27]Kodama T,Rokuda M,Park KS,Cantarelli VV,Matsuda S,Iida T,Honda T.Identification and characterization of VopT,a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2.Cellular Microbiology,2007,9(11):2598-2609.
[28]Casselli T,Lynch T,Southward CM,Jones BW,de Vinney R.Vibrio parahaemolyticus inhibition of Rho family GTPase activation requires a functional chromosome I type IIIsecretion system.Infection&Immunity,2008,76(5):2202-2211.
[29]Zhou XH,Gewurz BE,Ritchie JM,Takasaki K,Greenfeld H,Kieff E,Davis BM,Waldor MK.A Vibrio parahaemolyticus T3SS effector mediates pathogenesis by independently enabling intestinal colonization and inhibiting TAK1activation.Cell Reports,2013,3(5):1690-1702.
[30]Tsai SE,Jong KJ,Tey YH,Yu WT,Chiou CS,Lee YS,Wong HC.Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Taiwan.International Journal of Food Microbiology,2013,165(1):18-26.
[31]Calder T,Kinch LN,Fernandez J,Salomon D,Grishin NV,Orth K.Vibrio type III effector VPA1380 is related to the cysteine protease domain of large bacterial toxins.PLoS ONE,2014,9(8):e104387.
[32]李楚楚.副溶血弧菌T3SS2新效应蛋白的鉴定及其功能的初步研究.浙江理工大学硕士学位论文,2016.
[33]Kodama T,Hiyoshi H,Gotoh K,Akeda Y,Matsuda S,Park KS,Cantarelli VV,Iida T,Honda T.Identification of two translocon proteins of Vibrio parahaemolyticus type IIIsecretion system 2.Infection&Immunity,2008,76(9):4282-4289.
[34]Zhou XH,Konkel ME,Call DR.Regulation of type IIIsecretion system 1 gene expression in Vibrio parahaemolyticus is dependent on interactions between ExsA,ExsC,and ExsD.Virulence,2010,1(4):260-272.
[35]Liu AC,Thomas NA.Transcriptional profiling of Vibrio parahaemolyticus exsA reveals a complex activation network for type III secretion.Frontiers in Microbiology,2015,6:1089.
[36]Osei-Adjei G,Gao H,Zhang Y,Zhang LY,Yang WH,Yang HY,Yin Z,Huang XX,Zhang YQ,Zhou DS.Regulatory actions of ToxR and CalR on their own genes and type IIIsecretion system 1 in Vibrio parahaemolyticus.Oncotarget,2017,8:65809-65822.
[37]Li P,Rivera-Cancel G,Kinch LN,Salomon D,Tomchick DR,Grishin NV,Orth K.Bile salt receptor complex activates a pathogenic type III secretion system.eLife,2016,5:e15718.
[38]Pukatzki S,Ma AT,Revel AT,Sturtevant D,Mekalanos JJ.Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin.Proceedings of the National Academy of Sciences of the United States of America,2007,104(39):15508-15513.
[39]Mougous JD,Cuff ME,Raunser S,Shen A,Zhou M,Gifford CA,Goodman AL,Joachimiak G,Ordo?ez CL,Lory S,Walz T,Joachimiak A,Mekalanos JJ.A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.Science,2006,312(5779):1526-1530.
[40]Pukatzki S,Ma A T,Sturtevant D,Krastins B,Sarracino D,Nelson WC,Heidelberg JF,Mekalanos JJ.Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system.Proceedings of the National Academy of Sciences of the United States of America,2006,103(5):1528-1533.
[41]Salomon D,Gonzalez H,Updegraff BL,Orth K.Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria,and is differentially regulated from system 2.PLoS One,2013,8(4):e61086.
[42]Yu Y,Yang H,Li J,Zhang PP,Wu BB,Zhu BL,Zhang Y,Fang WH.Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers.Archives of Microbiology,2012,194(10):827-835.
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