Diversity of ds RNA Viruses Infecting Rice Sheath Blight Fungus Rhizoctonia solani AG-1 IA
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摘要
Rice sheath blight,caused by Rhizoctonia solani(Kühn),is a notorious soil-borne disease prevalent in many rice-growing regions.Although several sporadic studies of mycoviruses in R.solani AG-1 IA have been reported for single strain of R.solani AG-1 IA,there have been no reports describing the distribution and diversity of mycoviruses in natural populations.In this study,43 R.solani AG-1 IA strains collected from different locations in China were examined for the presence of ds RNA elements to confirm the presence of viral infections.Electrophoretypes showed that 16 of the 43 fungal strains(37.2%)contained ds RNAs that can be characterized as viruses.Furthermore,the species-specific reverse transcription PCR(RT-PCR)showed ds RNA bands with similar sizes do not always contain the same virus but exist as mixed mycoviral infections.Thus,our findings indicate mycoviruses infecting R.solani AG-1 IA in China are diverse,widespread and universal.
Rice sheath blight,caused by Rhizoctonia solani(Kühn),is a notorious soil-borne disease prevalent in many rice-growing regions.Although several sporadic studies of mycoviruses in R.solani AG-1 IA have been reported for single strain of R.solani AG-1 IA,there have been no reports describing the distribution and diversity of mycoviruses in natural populations.In this study,43 R.solani AG-1 IA strains collected from different locations in China were examined for the presence of ds RNA elements to confirm the presence of viral infections.Electrophoretypes showed that 16 of the 43 fungal strains(37.2%)contained ds RNAs that can be characterized as viruses.Furthermore,the species-specific reverse transcription PCR(RT-PCR)showed ds RNA bands with similar sizes do not always contain the same virus but exist as mixed mycoviral infections.Thus,our findings indicate mycoviruses infecting R.solani AG-1 IA in China are diverse,widespread and universal.
Rice sheath blight,caused by Rhizoctonia solani(Kühn),is a notorious soil-borne disease prevalent in many rice-growing regions.Although several sporadic studies of mycoviruses in R.solani AG-1 IA have been reported for single strain of R.solani AG-1 IA,there have been no reports describing the distribution and diversity of mycoviruses in natural populations.In this study,43 R.solani AG-1 IA strains collected from different locations in China were examined for the presence of ds RNA elements to confirm the presence of viral infections.Electrophoretypes showed that 16 of the 43 fungal strains(37.2%)contained ds RNAs that can be characterized as viruses.Furthermore,the species-specific reverse transcription PCR(RT-PCR)showed ds RNA bands with similar sizes do not always contain the same virus but exist as mixed mycoviral infections.Thus,our findings indicate mycoviruses infecting R.solani AG-1 IA in China are diverse,widespread and universal.
引文
Bartholom?us A,Wibberg D,Winkler A,Pühler A,Schlüter A,Varrelmann M.2016.Deep sequencing analysis reveals the mycoviral diversity of the virome of an avirulent isolate of Rhizoctonia solani AG-2-2 IV.PLo S One,11(11):e0165965.
Bharathan N,Saso H,Gudipati L,Bharathan S,Whited K,Anthony K.2005.Double-stranded RNA distribution and analysis among isolates of Rhizoctonia solani AG-2 to-13.Plant Pathol,54(2):196-203.
Castanho B,Butler E E.1978.Rhizoctonia decline:A degenerative disease of Rhizoctonia solani.Phytopathology,68:1505-1510.
Das S,Falloon R E,Stewart A,Pitman A R.2016.Novel mitoviruses in Rhizoctonia solani AG-3PT infecting potato.Fungal Biol,120(3):338-350.
Ghabrial S A.1998.Origin,adapation and evolutionary pathways of fungal viruses.Virus Genes,16(1):119-131.
Ghabrial S A,Suzuki N.2008.Fungal viruses.In:Mahy B W J,van Regenmortel M H V.Encyclopedia of Virology.3rd edn.Oxford:Elsevier:284-291.
Ghabrial S A,Suzuki N.2009.Viruses of plant pathogenic fungi.Annu Rev Phytopathol,47:353-384.
Lin Y H,Chiba S,Tani A,Kondo H,Sasaki A,Kanematsu S,Suzuki N.2012.A novel quadripartite ds RNA virus isolated from a phytopathogenic filamentous fungus,Rosellinia necatrix.Virology,426(1):42-50.
Liu L J,Xie J T,Cheng J S,Fu Y P,Li G Q,Yi X H,Jiang D H.2014.Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses.Proc Natl Acad Sci USA,111(33):12205-12210.
Morris T J,Dodds J A.1979.Isolation and analysis of double stranded RNA from virus infected plant and fungal tissue.Phytopathology,69(8):854-858.
Nuss D L.2005.Hypovirulence:Mycoviruses at the fungal-plant interface.Nat Rev Microbiol,3:632-642.
Nuss D L.2010.Mycoviruses.In:Borkovich K A.Cellular and Molecular Biology of Filamentous Fungi.Washington,USA:American Society of Microbiology:145-152.
Ogoshi A.1987.Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kühn.Annu Rev Phytopathol,25:125-143.
Okada R,Kiyota E,Sabanadzovic S,Moriyama H,Fukuhara T,Saha P,Roossinck M J,Severin A,Valverde R A.2011.Bell pepper endornavirus:Molecular and biological properties,and occurrence in the genus Capsicum.J Gen Virol,92:2664-2673.
Pearson M N,Beever R E,Boine B,Arthur K.2009.Mycoviruses of filamentous fungi and their relevance to plant pathology.Mol Plant Pathol,10(1):115-128.
Vainio E J,Hyder R,Aday G,Hansen E,Piri T,Dogmus-Lehtijarvi T,Lehtijarvi A,Korhonen K,Hantula J.2012.Population structure of a novel putative mycovirus infecting the conifer root-rot fungus Heterobasidium annosum sensu lato.Virology,422(2):366-376.
Yu X,Li B,Fu Y P,Jiang D H,Ghabrial S A,Li G Q,Peng Y L,Xie J T,Cheng J S,Huang J,Yi X H.2010.A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus.Proc Natl Acad Sci USA,107(18):8387-8392.
Yu X,Li B,Fu Y P,Xie J T,Cheng J S,Ghabrial S A,Li G Q,Yi XH,Jiang D H.2013.Extracellular transmission of a DNAmycovirus and its use as a nature fungicide.Proc Natl Acad Sci USA,110(4):1452-1457.
Zhang D T,Peng Z K,Cao Q Q,Yang M,Zheng L,Zhou E X.2012.Colonization of three antagonistic strains on rice plant and their biocontrol effects on rice sheath blight.J Northwest Agric Forest Univ,40:97-102.(in Chinese with English abstract)
Zheng L,Liu H Q,Zhang M L,Cao X,Zhou E X.2013.The complete genomic sequence of a novel mycovirus from Rhizoctonia solani AG-1 IA strain B275.Arch Virol,158(7):1609-1612.
Zheng L,Zhang M L,Chen Q G,Zhu M H,Zhou E X.2014.Anovel mycovirus closely related to viruses in the genus Alphapartitivirus confers hypovirulence in the phytopathogenic fungus Rhizoctonia solani.Virology,456/457(4):220-226.
Zhong J,Chen C Y,Gao B D.2015.Genome sequence of a novel mycovirus of Rhizoctonia solani,a plant pathogenic fungus.Virus Genes,51(1):167-170.
Bharathan N,Saso H,Gudipati L,Bharathan S,Whited K,Anthony K.2005.Double-stranded RNA distribution and analysis among isolates of Rhizoctonia solani AG-2 to-13.Plant Pathol,54(2):196-203.
Castanho B,Butler E E.1978.Rhizoctonia decline:A degenerative disease of Rhizoctonia solani.Phytopathology,68:1505-1510.
Das S,Falloon R E,Stewart A,Pitman A R.2016.Novel mitoviruses in Rhizoctonia solani AG-3PT infecting potato.Fungal Biol,120(3):338-350.
Ghabrial S A.1998.Origin,adapation and evolutionary pathways of fungal viruses.Virus Genes,16(1):119-131.
Ghabrial S A,Suzuki N.2008.Fungal viruses.In:Mahy B W J,van Regenmortel M H V.Encyclopedia of Virology.3rd edn.Oxford:Elsevier:284-291.
Ghabrial S A,Suzuki N.2009.Viruses of plant pathogenic fungi.Annu Rev Phytopathol,47:353-384.
Lin Y H,Chiba S,Tani A,Kondo H,Sasaki A,Kanematsu S,Suzuki N.2012.A novel quadripartite ds RNA virus isolated from a phytopathogenic filamentous fungus,Rosellinia necatrix.Virology,426(1):42-50.
Liu L J,Xie J T,Cheng J S,Fu Y P,Li G Q,Yi X H,Jiang D H.2014.Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses.Proc Natl Acad Sci USA,111(33):12205-12210.
Morris T J,Dodds J A.1979.Isolation and analysis of double stranded RNA from virus infected plant and fungal tissue.Phytopathology,69(8):854-858.
Nuss D L.2005.Hypovirulence:Mycoviruses at the fungal-plant interface.Nat Rev Microbiol,3:632-642.
Nuss D L.2010.Mycoviruses.In:Borkovich K A.Cellular and Molecular Biology of Filamentous Fungi.Washington,USA:American Society of Microbiology:145-152.
Ogoshi A.1987.Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kühn.Annu Rev Phytopathol,25:125-143.
Okada R,Kiyota E,Sabanadzovic S,Moriyama H,Fukuhara T,Saha P,Roossinck M J,Severin A,Valverde R A.2011.Bell pepper endornavirus:Molecular and biological properties,and occurrence in the genus Capsicum.J Gen Virol,92:2664-2673.
Pearson M N,Beever R E,Boine B,Arthur K.2009.Mycoviruses of filamentous fungi and their relevance to plant pathology.Mol Plant Pathol,10(1):115-128.
Vainio E J,Hyder R,Aday G,Hansen E,Piri T,Dogmus-Lehtijarvi T,Lehtijarvi A,Korhonen K,Hantula J.2012.Population structure of a novel putative mycovirus infecting the conifer root-rot fungus Heterobasidium annosum sensu lato.Virology,422(2):366-376.
Yu X,Li B,Fu Y P,Jiang D H,Ghabrial S A,Li G Q,Peng Y L,Xie J T,Cheng J S,Huang J,Yi X H.2010.A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus.Proc Natl Acad Sci USA,107(18):8387-8392.
Yu X,Li B,Fu Y P,Xie J T,Cheng J S,Ghabrial S A,Li G Q,Yi XH,Jiang D H.2013.Extracellular transmission of a DNAmycovirus and its use as a nature fungicide.Proc Natl Acad Sci USA,110(4):1452-1457.
Zhang D T,Peng Z K,Cao Q Q,Yang M,Zheng L,Zhou E X.2012.Colonization of three antagonistic strains on rice plant and their biocontrol effects on rice sheath blight.J Northwest Agric Forest Univ,40:97-102.(in Chinese with English abstract)
Zheng L,Liu H Q,Zhang M L,Cao X,Zhou E X.2013.The complete genomic sequence of a novel mycovirus from Rhizoctonia solani AG-1 IA strain B275.Arch Virol,158(7):1609-1612.
Zheng L,Zhang M L,Chen Q G,Zhu M H,Zhou E X.2014.Anovel mycovirus closely related to viruses in the genus Alphapartitivirus confers hypovirulence in the phytopathogenic fungus Rhizoctonia solani.Virology,456/457(4):220-226.
Zhong J,Chen C Y,Gao B D.2015.Genome sequence of a novel mycovirus of Rhizoctonia solani,a plant pathogenic fungus.Virus Genes,51(1):167-170.