百合地黄汤对失眠模型大鼠行为学及不同脑区单胺类神经递质的影响
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摘要
目的:研究百合地黄汤对失眠模型大鼠行为学及大鼠皮层、脑干和海马组织中多巴胺(DA)和5-羟色胺(5-HT)的影响,探讨百合地黄汤治疗失眠的作用机制。方法:采用腹腔注射对氯苯丙氨酸(PCPA,0.4 g·kg~(-1))复制失眠模型大鼠,将造模成功的大鼠随机分为模型组、地西泮组(0.92 mg·kg~(-1))、百合地黄汤高剂量组(28 g·kg~(-1))和百合地黄汤低剂量组(7 g·kg~(-1)),另设空白组,连续给药7天,采用旷场实验测定行为学评分,翻正反射实验评价百合地黄汤对大鼠睡眠的影响,高效液相色谱电化学检测法(HPLC-ECD)检测大鼠皮层、脑干和海马中DA和5-HT的含量。结果:百合地黄汤可以降低失眠模型大鼠的中央格停留时间、修饰次数和粪便颗粒数(P <0.05),缩短睡眠潜伏期并延长睡眠持续时间(P <0.05),大脑皮层、脑干和海马中5-HT含量明显升高(P <0.05),大鼠皮层中DA含量显著降低(P <0.05);与模型组相比,P <0.05。结论:百合地黄汤可改善失眠模型大鼠行为学和睡眠,对DA和5-HT的调节作用可能是其治疗失眠的作用机制。
Objective: To study the effects of Baihe Dihuang Decoction on the ethology of insomnia model rats and dopamine(DA) and serotonin(5-HT) in the cortex, brain stem and hippocampus of rats, so as to study the mechanism of Baihe Dihuang Decoction in the treatment of insomnia. Methods: Rats were given parachlorophenylalanine(PCPA, 0.4 g·kg~(-1))to replicate insomnia model, and were randomly divided into model group, diazepam group(0.92 mg·kg~(-1)), Baihe Dihuang Decoction high dose group(28 g·kg~(-1)) and Baihe Dihuang Decoction low dose group(7 g·kg~(-1)), and blank group.All groups were administrated continuously for 7 days, The behavior score was measured by open field test, and the sleeping of rats was evaluated by reversal reflex experiment. The contents of DA and 5-HT in the cortex, brain stem and hippocampus of rats were measured by high performance liquid chromatography with electrochemical detection method(HPLC-ECD). Results: Baihe Dihuang Decoction can reduce the time in center grid, the number of fecal particles and grooming(P < 0.05), shorten the sleep latency and prolong the duration of sleeping quality(P < 0.05), and the 5-HT content in the cortex, brain stem and hippocampus of insomnia model rats increased significantly(P < 0.05). The content of DA in rat cortex decreased significantly(P < 0.05), compared with the model group, P < 0.05. Conclusion: Baihe Dihuang Decoction can improve the ethology and sleeping quality of insomnia rats, and its regulating effect on DA and 5-HT may be the mechanism of insomnia treatment.
Objective: To study the effects of Baihe Dihuang Decoction on the ethology of insomnia model rats and dopamine(DA) and serotonin(5-HT) in the cortex, brain stem and hippocampus of rats, so as to study the mechanism of Baihe Dihuang Decoction in the treatment of insomnia. Methods: Rats were given parachlorophenylalanine(PCPA, 0.4 g·kg~(-1))to replicate insomnia model, and were randomly divided into model group, diazepam group(0.92 mg·kg~(-1)), Baihe Dihuang Decoction high dose group(28 g·kg~(-1)) and Baihe Dihuang Decoction low dose group(7 g·kg~(-1)), and blank group.All groups were administrated continuously for 7 days, The behavior score was measured by open field test, and the sleeping of rats was evaluated by reversal reflex experiment. The contents of DA and 5-HT in the cortex, brain stem and hippocampus of rats were measured by high performance liquid chromatography with electrochemical detection method(HPLC-ECD). Results: Baihe Dihuang Decoction can reduce the time in center grid, the number of fecal particles and grooming(P < 0.05), shorten the sleep latency and prolong the duration of sleeping quality(P < 0.05), and the 5-HT content in the cortex, brain stem and hippocampus of insomnia model rats increased significantly(P < 0.05). The content of DA in rat cortex decreased significantly(P < 0.05), compared with the model group, P < 0.05. Conclusion: Baihe Dihuang Decoction can improve the ethology and sleeping quality of insomnia rats, and its regulating effect on DA and 5-HT may be the mechanism of insomnia treatment.
引文
1李丽娜.近10年中医药治疗失眠症的研究进展.中医药信息, 2018,35(3):119-123.
2 武润梅,王芝平,梁晶晶,等.失眠综述及临证举例.光明中医, 2017,32(7):939-941.
3 瞿萍,陈贵海.失眠的药物治疗.临床药物治疗杂志,2012,10(2):50-54
4 汉·张仲景.金匮要略.北京:人民卫生出版社, 1963:8.
5 王东旭,王鹏琴.眼针疗法联合百合地黄汤加减治疗阴虚火旺型不寐30例临床观察.湖南中医杂志, 2018, 34(3):89-90.
6 利顺欣.百合地黄汤合酸枣仁汤加味治疗老年失眠.中国中医药现代远程教育, 2014, 12(20):48-49.
7 王亚渭.针刺联合百合地黄汤加味治疗失眠临床研究.陕西中医学院学报, 2015, 38(4):50-51.
8 孟彦,贾怡,武嫣斐,等.百合地黄汤在神经精神系统中的应用研究进展.中草药, 2018, 49(1):251-255.
9 管家齐,孙燕,陈文东.百合地黄汤对抑郁模型小鼠脑内单胺类神经递质的影响.中国实验方剂学杂志, 2010, 16(9):131-133.
10 Man J, Ming-Xian C, Kai-Sheng Y. Beneficial effects of jiawei Baihe Dihuang Tang, a Chinese herbal decoction, in mouse hepatocellular carcinoma. Int J Clin Exp Med, 2016, 9(7):13148-13153.
11 王月洪,阿古拉,斯楞格,等.蒙医温针对大鼠睡眠时间和自主活动的影响.上海针灸杂志, 2014, 33(5):472-475.
12 胡金凤.“针刺五脏俞调五脏神”针法干预失眠机制的实验研究.黑龙江:黑龙江中医药大学博士研究生学位论文, 2007.
13 宋媛,赵仓焕,任莉.不同穴位处方麦粒灸对失眠大鼠下丘脑细胞因子IL-1β、TNF-α、IL-6含量的影响.中国中医基础医学杂志, 2009,15(4):304-305.
14 陈汉裕,陈凤丽,林赞檬,等.黄连阿胶汤对戊巴比妥钠致小鼠催眠作用及神经递质的影响.广东医学, 2016, 37(21):3165-3168.
15 Borbely A A, Neuhaus H U, Tobler I. Effect of p-chlorophenylalanine and tryptophan on sleep, EEG and motor activity in the rat. Behav Brain Res, 1981, 2(1):1-22.
16 徐福平,黄鹂,原嘉民,等.舒心安神膏对睡眠剥夺大鼠脑组织中单胺类神经递质含量的影响.广东医学, 2015, 36(6):828-831.
17 姚秋菊,韩旭. 5-羟色胺与失眠的相关性及中医辨证治疗的研究进展.中医药导报, 2013, 19(2):108-110.
18 石皓月,鲁艺,李钰昕,等.中药治疗对氯苯丙氨酸失眠模型大鼠影响的基础研究进展.中国医药导报, 2018, 15(11):33-36.
19 吴雪芬,郑雪娜,郭鑫,等.针刺对失眠大鼠5-HT及其受体、HPA轴相关激素影响的研究进展.中华中医药学刊, 2018, 36(1):127-129.
20 王亚茹,于世家.于世家教授自拟安眠方对PCPA诱导失眠大鼠脑内递质及GABA的影响.时珍国医国药, 2018, 29(4):852-855.
21 郭鑫,岳增辉,谢菊英,等.针刺对失眠大鼠血清去甲肾上腺素、多巴胺及5-羟色胺含量的影响.中国中医药信息杂志, 2018, 25(4):46-50.
22 罗林明,裴刚,覃丽,等.中药百合化学成分及药理作用研究进展.中国新药与临床药理, 2017, 06(28):824-837.
23 刘卫欣,卢兖伟,杜海涛,等.地黄及其活性成分药理作用研究进展.国际药学研究杂志, 2009, 4(38):277-279.
2 武润梅,王芝平,梁晶晶,等.失眠综述及临证举例.光明中医, 2017,32(7):939-941.
3 瞿萍,陈贵海.失眠的药物治疗.临床药物治疗杂志,2012,10(2):50-54
4 汉·张仲景.金匮要略.北京:人民卫生出版社, 1963:8.
5 王东旭,王鹏琴.眼针疗法联合百合地黄汤加减治疗阴虚火旺型不寐30例临床观察.湖南中医杂志, 2018, 34(3):89-90.
6 利顺欣.百合地黄汤合酸枣仁汤加味治疗老年失眠.中国中医药现代远程教育, 2014, 12(20):48-49.
7 王亚渭.针刺联合百合地黄汤加味治疗失眠临床研究.陕西中医学院学报, 2015, 38(4):50-51.
8 孟彦,贾怡,武嫣斐,等.百合地黄汤在神经精神系统中的应用研究进展.中草药, 2018, 49(1):251-255.
9 管家齐,孙燕,陈文东.百合地黄汤对抑郁模型小鼠脑内单胺类神经递质的影响.中国实验方剂学杂志, 2010, 16(9):131-133.
10 Man J, Ming-Xian C, Kai-Sheng Y. Beneficial effects of jiawei Baihe Dihuang Tang, a Chinese herbal decoction, in mouse hepatocellular carcinoma. Int J Clin Exp Med, 2016, 9(7):13148-13153.
11 王月洪,阿古拉,斯楞格,等.蒙医温针对大鼠睡眠时间和自主活动的影响.上海针灸杂志, 2014, 33(5):472-475.
12 胡金凤.“针刺五脏俞调五脏神”针法干预失眠机制的实验研究.黑龙江:黑龙江中医药大学博士研究生学位论文, 2007.
13 宋媛,赵仓焕,任莉.不同穴位处方麦粒灸对失眠大鼠下丘脑细胞因子IL-1β、TNF-α、IL-6含量的影响.中国中医基础医学杂志, 2009,15(4):304-305.
14 陈汉裕,陈凤丽,林赞檬,等.黄连阿胶汤对戊巴比妥钠致小鼠催眠作用及神经递质的影响.广东医学, 2016, 37(21):3165-3168.
15 Borbely A A, Neuhaus H U, Tobler I. Effect of p-chlorophenylalanine and tryptophan on sleep, EEG and motor activity in the rat. Behav Brain Res, 1981, 2(1):1-22.
16 徐福平,黄鹂,原嘉民,等.舒心安神膏对睡眠剥夺大鼠脑组织中单胺类神经递质含量的影响.广东医学, 2015, 36(6):828-831.
17 姚秋菊,韩旭. 5-羟色胺与失眠的相关性及中医辨证治疗的研究进展.中医药导报, 2013, 19(2):108-110.
18 石皓月,鲁艺,李钰昕,等.中药治疗对氯苯丙氨酸失眠模型大鼠影响的基础研究进展.中国医药导报, 2018, 15(11):33-36.
19 吴雪芬,郑雪娜,郭鑫,等.针刺对失眠大鼠5-HT及其受体、HPA轴相关激素影响的研究进展.中华中医药学刊, 2018, 36(1):127-129.
20 王亚茹,于世家.于世家教授自拟安眠方对PCPA诱导失眠大鼠脑内递质及GABA的影响.时珍国医国药, 2018, 29(4):852-855.
21 郭鑫,岳增辉,谢菊英,等.针刺对失眠大鼠血清去甲肾上腺素、多巴胺及5-羟色胺含量的影响.中国中医药信息杂志, 2018, 25(4):46-50.
22 罗林明,裴刚,覃丽,等.中药百合化学成分及药理作用研究进展.中国新药与临床药理, 2017, 06(28):824-837.
23 刘卫欣,卢兖伟,杜海涛,等.地黄及其活性成分药理作用研究进展.国际药学研究杂志, 2009, 4(38):277-279.