Study on Extraction Method of Selenium-enriched Rice
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摘要
[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS( atomic fluorescence spectrometry) respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.
[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS( atomic fluorescence spectrometry) respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.
[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS( atomic fluorescence spectrometry) respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production.
引文
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[10]ZHU JQ,LI SF,YU L.Optimization of alkaline extraction joint microwave and enzymatic modification of rice bran protein[J].Cereals and Oils,2017,30(7):60-64.
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[15]ZHANG S,ZHANG PY,FENG CP.Extraction of Chinese chestnut protein and preparation of the polypeptides[J].Journal of Shanxi Agricultural University(Natural Science Edition),2011,52(1):73-76.
[16]JIANG AZ,WANG Y.Research advance on extraction methods of rice bran protein[J].Journal of Cereals and Oils,2009(10):11-12.
[17]DENG GC,HOU SM,TIAN DM,et al.Separation and determination of selenium polysaccharide in selenium-enriched Cordyceps militaris[J].Spectroscopy and Spectral Analysis,2006,26(3):522-525.
[18]FANG Y,ZHANG YF,BRITTANY C,et al.Identification of selenium compounds using HPLC-ICPMS and nano-ESI-MS in selenium-enriched rice via folia application[J].Journal of Analytical Atomic Spectrometry,2009,24(12):1657-1664.
[19]CAO B.Study on extraction and separation,preliminary purification and antioxidant activity of selenium-containing protein from Cardamine Enshiensis[D].Guizhou University,2016:35-50.
[20]WANG L,YANG T,JIANG T,et al.Effects of different extraction methods on functional properties of rice protein[J].The Food Industry,2014,35(10):60-63.
[21]WANG F,LIU H,DONG MH.Functional properties of proteins from mulberry leaves[J].Food Science,2010,31(11):81-86.
[22]JIANG ZW,SHEN BY.Protein processing technology[M].Shanghai:Chemical Industry Press,2003:192-193.
[23]LV J.Extraction,functional properties and application of Chinese chestnut protein[D].Beijing:Beijing Forestry University,2015:15-18.
[24]LI XH,DAI HL,WEI AC.Extracting proteins from defatted rice bran by enzymatic method[J].Machinery for Cereals and Food Processing,2005(6):62-64.
[2]ZHANG T.The selenium speciation analysis in the selenium-rich rice and selenized yeast[D].Baotou:Inner Mongolia University of Science and Technology,2007:28-34.
[3]FANG JJ,ZHU HM,FANG F,et al.Analysis of selenium forms in selenium-enriched rice[J].Food Research and Development,2012,33(9):146-150.
[4]LI T.Distribution of selenium,preparation and antioxidant activity of Selenium-enriched protein from Xinchun No.36 triticale[D].Master's thesis in Shihezi University,2016:11-14.
[5]WANG J,DONG WB,YANG FL,et al.Investigation on selenium-protein from selenium-enriched tea by repeated freezing and thawing alkali extraction[J].Food and Machinery,2015(2):211-215.
[6]ZOU XR,ZHU JH,CAO SM.Study on rice bran protein by ultrasonicassisted extraction[J].Science and Technology of Food Industry,2013(16):280-282.
[7]GERGELY V,KUBACHKA KM,MOUNICOU S,et al.Selenium speciation in Agaricus bisporus and Lentinula edodes mushroom proteins using multi-dimensional chromatography coupled to inductively coupled plasma mass spectrometry[J].Journal of Chromatography A,2006,1101(1-2):94-102.
[8]GB/T 5009.93-2003.Determination of selenium in foods[S].
[9]PENG W.Extraction of selenoproteins from Selenium-enriched Pleurotus ostreatus and development of special dietary foods[D].Guizhou University,2016:16-19.
[10]ZHU JQ,LI SF,YU L.Optimization of alkaline extraction joint microwave and enzymatic modification of rice bran protein[J].Cereals and Oils,2017,30(7):60-64.
[11]HAN SW,CHEE KM,CHO SJ.Nutritional quality of rice bran protein in comparison to animal and vegetable protein[J].Food Chemistry,2015,172:766-769.
[12]ZHENG YY,ZENG J,LI J,et al.Composition and functional properties of rice bran proteins[J].Food Science,2012,33(23):143-149.
[13]FANG Y,CARTRON B,ZHANG Y,et al.Distribution and in vitro availability of selenium in selenium-containing storage protein from selenium-enriched rice utilizing optimized extraction[J].Journal of Agricultural and Food Chemistry,2010,58(17):9731-9738.
[14]HU QH,CHEN X,FANG Y,et al.Study of optimization of preparation of Selenium-enriched rice bran protein and its nutritive compound of mixed proteins[J].Scientia Agricultura Sinica,2014,47(2):374-382.
[15]ZHANG S,ZHANG PY,FENG CP.Extraction of Chinese chestnut protein and preparation of the polypeptides[J].Journal of Shanxi Agricultural University(Natural Science Edition),2011,52(1):73-76.
[16]JIANG AZ,WANG Y.Research advance on extraction methods of rice bran protein[J].Journal of Cereals and Oils,2009(10):11-12.
[17]DENG GC,HOU SM,TIAN DM,et al.Separation and determination of selenium polysaccharide in selenium-enriched Cordyceps militaris[J].Spectroscopy and Spectral Analysis,2006,26(3):522-525.
[18]FANG Y,ZHANG YF,BRITTANY C,et al.Identification of selenium compounds using HPLC-ICPMS and nano-ESI-MS in selenium-enriched rice via folia application[J].Journal of Analytical Atomic Spectrometry,2009,24(12):1657-1664.
[19]CAO B.Study on extraction and separation,preliminary purification and antioxidant activity of selenium-containing protein from Cardamine Enshiensis[D].Guizhou University,2016:35-50.
[20]WANG L,YANG T,JIANG T,et al.Effects of different extraction methods on functional properties of rice protein[J].The Food Industry,2014,35(10):60-63.
[21]WANG F,LIU H,DONG MH.Functional properties of proteins from mulberry leaves[J].Food Science,2010,31(11):81-86.
[22]JIANG ZW,SHEN BY.Protein processing technology[M].Shanghai:Chemical Industry Press,2003:192-193.
[23]LV J.Extraction,functional properties and application of Chinese chestnut protein[D].Beijing:Beijing Forestry University,2015:15-18.
[24]LI XH,DAI HL,WEI AC.Extracting proteins from defatted rice bran by enzymatic method[J].Machinery for Cereals and Food Processing,2005(6):62-64.