MTDH和Wnt5b在乙肝相关性肝癌组织中的表达和意义
摘要
目的观察异黏蛋白(metadherin,MTDH)与Wnt5b在乙肝相关性肝细胞癌患者中的表达及其意义,对乙肝相关性肝癌发病的作用机制进行初步探讨。方法采用免疫组织化学方法及逆转录PCR(RT-PCR)法检测54名肝癌患者及2名正常肝脏患者中MTDH和Wnt5b的表达情况,通过分析,探讨MTDH和Wnt5b表达水平与乙肝相关HCC的病理特征的相关性。结果 54例患者在乙肝相关性肝癌中MTDH表达阳性率为77.78%,Wnt5b的阳性表达率为68.51%,正常肝组织中几乎未见MTDH表达,而Wnt5b却在正常肝组织中高表达;并且MTDH和Wnt5b在乙肝相关性肝癌HCC组织中的表达与患者的年龄、性别无相关性(P>0.05),但与肿瘤直径有相关性(P<0.05)。结论 MTDH与Wnt5b共同参与HCC的进展,具有早期诊断评估HCC进展程度的价值,为乙肝相关性肝癌的诊治提供新的思路。
引文
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[4]叶胜龙.2014年肝细胞癌研究进展回顾[J].中华肝脏病杂志,2015,23(2):90-93.
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[6]陈万青,张思维,曾红梅,等.中国2010年恶性肿瘤发病与死亡[J].中国肿瘤,2014,23(1):1-9.
[7]魏矿荣,彭侠彪,梁智恒,等.全球肝癌流行概况[J].中国肿瘤,2015,24(8):621-630.
[8]沈福军.乙肝相关性肝癌抗病毒治疗临床研究[J].肝胆外科杂志,2016,24(2):135-138.
[9]朱瑜.AFP、CEA、CA199诊断乙型肝炎相关肝癌初步研究[J].实验与检验医学,2011,29(5):541-542.
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[11]Mehravib,Ahmadim,Amanloum,et al.Cellular uptake and imaging studies of glycosylated silica nanoprobe(GSN)in human colon adenocarcinoma(HT 29 cell line)[J].Int J Nanomedicine,2013,8(10):3209-3216.
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[13]Mcglynn KA,London WT.The global epidemiology of hepatocellular carcinoma:present and future[J].Clin Liver Dis,2011,15(2):223-243.
[14]沈福军.乙肝相关性肝癌抗病毒治疗临床研究[J].肝胆外科杂志,2016,24(2):135-138.
[15]Baldwin AS,Jr.The NF-kappa B and I kappa B proteins:new discoveries and insights[J].Annu Rev Immunol,1996,14(1):649-683.
[16]Yuan G,Regel I,Lian F,et al.WNT6 is a novel target gene of caveolin-1 promoting chemoresistance to epirubicin in human gastric cancer cells[J].Oncogene,2013,32(3):375-387.
[17]Cui L,Guan Y,Qu Z,et al.WNT signaling determines tumorigenicity and function of ESCderived retinal progenitors[J].Clin Invest,2013,123(4):1647-1661.
[18]Wands JR,Kim M.WNT/beta-catenin signaling and hepatocellular carcinoma[J].Hepatology,2014,60(2):452-454.
[19]Liu F,Millar SE.Wnt/beta-catenin signaling in oral tissue development and disease[J].Dental Res,2010,89(4):318-330.
[20]Gabrovska PN,Smith RA,Tiang T,et al.Development of an eight gene expression profile implicating human breast tumours of all grade[J].Mol Biol Rep,2012,39(4):3879-3892.