PCR-RFLP鉴别胡氏苘麻和苘麻
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摘要
本研究对胡氏苘麻和苘麻的核糖体ITS进行PCR-RFLP分析,首次从分子水平上鉴定区分了这两种。根据这两个种的ITS测序结果,选取了限制性内切酶Mse I应用于PCR-RFLP分析。结果表明根据酶切图谱可将这两种的ITS扩增产物区分开。为建立PCR-RFLP分子鉴定方法有效区分胡氏苘麻和苘麻提供依据。
With PCR-RFLP analysis in Abutilon hulseanum and A. theophrasti rDNA ITS region,the molecular level was first identified based on the sequencing result in this study,the restriction enzyme Mse I was selected for PCR-RFLP analysis. Results showed that the amplified ITS products of the two species can be distinguished based on the restriction map. Therefore,this PCR-RFLP method established in this study can be used as the basis for identification between A. hulseanum and A. theophrasti.
With PCR-RFLP analysis in Abutilon hulseanum and A. theophrasti rDNA ITS region,the molecular level was first identified based on the sequencing result in this study,the restriction enzyme Mse I was selected for PCR-RFLP analysis. Results showed that the amplified ITS products of the two species can be distinguished based on the restriction map. Therefore,this PCR-RFLP method established in this study can be used as the basis for identification between A. hulseanum and A. theophrasti.
引文
[1]翟会锋,胡长松,朱林,等.中国口岸首次监测外来杂草-胡氏苘麻.植物检疫,2018,32(5):43-45.
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[3]高必达,程毅,朱水芳,等.基于ITS序列的菟丝子PCR鉴定.湖南农业大学学报(自然科学版),2006,32(4):368-370.
[4]郭琼霞,黄可辉,虞赟,等.基于rDNA ITS分析的假高粱鉴定方法.福建农业学报,2006,21(1):32-34.
[5]胡长松,陈瑞辉,翟会锋,等.隔离种植在杂草草检疫中的应用探讨.植物检疫,2017,31(6):23-25.
[6]伏建国,杨晓军,钱路,等.植物DNA条形码技术在出入境检验检疫领域的应用.植物检疫,2012,26(2):64-69.
[7]王师,包振民,张玲玲,等.应用PCR-RFLP技术鉴别区分中国沿海四种主要养殖扇贝.食品科学,2006,27(7):210-213.
[8]王柯,陈科力,刘震,等.锦葵科植物DNA条形码通用序列的筛选.植物学报,2011,46(3):276-284.
[9]郭琼霞,黄可辉,虞赟.PCR-RFLP鉴别假高粱和丝克高粱.植物检疫,2005,19(6):330-333.
[10]郭琼霞,黄可辉,虞赟,等.丝克高粱rDNA ITS区的RFLP分析.福建农林大学学报(自然科学版),2005,34(3):349-352.
[11]郑传进,赵树进,赵振华,等.基于rDNA ITS序列的何首乌PCR_RFLP分子鉴别.华南理工大学学报(自然科学版),2009,37(6):74-83.
[12]张姮,康林,高必达,等.毒麦及其近似种rDNA ITS-RFLP初步分析.植物检疫,2008,22(2):70-72.
[2]王建波,张文驹,陈家宽.核rDNA的ITS序列在被子植物系统与进化研究中的应用.植物分类学报,1999,37(4):407-416.
[3]高必达,程毅,朱水芳,等.基于ITS序列的菟丝子PCR鉴定.湖南农业大学学报(自然科学版),2006,32(4):368-370.
[4]郭琼霞,黄可辉,虞赟,等.基于rDNA ITS分析的假高粱鉴定方法.福建农业学报,2006,21(1):32-34.
[5]胡长松,陈瑞辉,翟会锋,等.隔离种植在杂草草检疫中的应用探讨.植物检疫,2017,31(6):23-25.
[6]伏建国,杨晓军,钱路,等.植物DNA条形码技术在出入境检验检疫领域的应用.植物检疫,2012,26(2):64-69.
[7]王师,包振民,张玲玲,等.应用PCR-RFLP技术鉴别区分中国沿海四种主要养殖扇贝.食品科学,2006,27(7):210-213.
[8]王柯,陈科力,刘震,等.锦葵科植物DNA条形码通用序列的筛选.植物学报,2011,46(3):276-284.
[9]郭琼霞,黄可辉,虞赟.PCR-RFLP鉴别假高粱和丝克高粱.植物检疫,2005,19(6):330-333.
[10]郭琼霞,黄可辉,虞赟,等.丝克高粱rDNA ITS区的RFLP分析.福建农林大学学报(自然科学版),2005,34(3):349-352.
[11]郑传进,赵树进,赵振华,等.基于rDNA ITS序列的何首乌PCR_RFLP分子鉴别.华南理工大学学报(自然科学版),2009,37(6):74-83.
[12]张姮,康林,高必达,等.毒麦及其近似种rDNA ITS-RFLP初步分析.植物检疫,2008,22(2):70-72.