In vivo hypoglycemic investigation, antihyperglycemic and antihyperlipidemic potentials of Pereskia bleo Kunth. in normal and streptozotocin-induced diabetic rats
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摘要
Objective: To elucidate the in vivo hypoglycemic capability, antihyperglycemic and antihyperlipidemic activities of Pereskia bleo(Kunth) leaves extracts and bioactive fraction. Methods: The various solvent extracts of Pereskia bleo were investigated for the hypoglycemic and antihyperglycemic activities using a relevant in vivo normal rat model and streptozotocininduced diabetic rat model with glibenclamide and metformin utilized as positive controls. The effects of the most potent extract and its bioactive fraction on the insulin level, lipid profile and body weight of the diabetic rats were also analyzed. Results: All the extracts showed no hypoglycemic effect while petroleum ether, chloroform and aqueous extracts demonstrated significant(P<0.05) reduction in blood sugar level in the intraperitoneal glucose tolerance test. Aqueous extract and aqueous fraction significantly(P<0.05) reduced the blood glucose level in streptozotocin-induced diabetic rats as early as day 6 compared to the diabetic control as well as significantly restored the serum insulin of diabetic rats. Moreover, the aqueous extract and aqueous fraction disclosed a significant(P<0.05) reduction in total cholesterol, triglycerides, and low-density lipoprotein levels. An elevation in high-density lipoprotein as well as improved body weight loss of the diabetic rats were also observed. Conclusions: In summary, Pereskia bleo appears effective in the management of diabetes and correlated impairments arising from high blood sugar level. Further studies will possibly bring about the discovery of effective and secure plant derived antidiabetic drugs.
Objective: To elucidate the in vivo hypoglycemic capability, antihyperglycemic and antihyperlipidemic activities of Pereskia bleo(Kunth) leaves extracts and bioactive fraction. Methods: The various solvent extracts of Pereskia bleo were investigated for the hypoglycemic and antihyperglycemic activities using a relevant in vivo normal rat model and streptozotocininduced diabetic rat model with glibenclamide and metformin utilized as positive controls. The effects of the most potent extract and its bioactive fraction on the insulin level, lipid profile and body weight of the diabetic rats were also analyzed. Results: All the extracts showed no hypoglycemic effect while petroleum ether, chloroform and aqueous extracts demonstrated significant(P<0.05) reduction in blood sugar level in the intraperitoneal glucose tolerance test. Aqueous extract and aqueous fraction significantly(P<0.05) reduced the blood glucose level in streptozotocin-induced diabetic rats as early as day 6 compared to the diabetic control as well as significantly restored the serum insulin of diabetic rats. Moreover, the aqueous extract and aqueous fraction disclosed a significant(P<0.05) reduction in total cholesterol, triglycerides, and low-density lipoprotein levels. An elevation in high-density lipoprotein as well as improved body weight loss of the diabetic rats were also observed. Conclusions: In summary, Pereskia bleo appears effective in the management of diabetes and correlated impairments arising from high blood sugar level. Further studies will possibly bring about the discovery of effective and secure plant derived antidiabetic drugs.
Objective: To elucidate the in vivo hypoglycemic capability, antihyperglycemic and antihyperlipidemic activities of Pereskia bleo(Kunth) leaves extracts and bioactive fraction. Methods: The various solvent extracts of Pereskia bleo were investigated for the hypoglycemic and antihyperglycemic activities using a relevant in vivo normal rat model and streptozotocininduced diabetic rat model with glibenclamide and metformin utilized as positive controls. The effects of the most potent extract and its bioactive fraction on the insulin level, lipid profile and body weight of the diabetic rats were also analyzed. Results: All the extracts showed no hypoglycemic effect while petroleum ether, chloroform and aqueous extracts demonstrated significant(P<0.05) reduction in blood sugar level in the intraperitoneal glucose tolerance test. Aqueous extract and aqueous fraction significantly(P<0.05) reduced the blood glucose level in streptozotocin-induced diabetic rats as early as day 6 compared to the diabetic control as well as significantly restored the serum insulin of diabetic rats. Moreover, the aqueous extract and aqueous fraction disclosed a significant(P<0.05) reduction in total cholesterol, triglycerides, and low-density lipoprotein levels. An elevation in high-density lipoprotein as well as improved body weight loss of the diabetic rats were also observed. Conclusions: In summary, Pereskia bleo appears effective in the management of diabetes and correlated impairments arising from high blood sugar level. Further studies will possibly bring about the discovery of effective and secure plant derived antidiabetic drugs.
引文
[1]World Health Organization(WHO).Global report on diabetes.Geneva:WHO Press;2016.[Online].Available from:http://apps.who.int/iris/bitstr eam/10665/204871/1/9789241565257_eng.pdf?ua=1
[2]Davies MJ,Fradkin J,Kernan WN,Mathieu C,Mingrone G,Rossing P,et al.Management of hyperglycaemia in type 2 diabetes,2018.A consensus report by the American Diabetes Association(ADA)and the European Association for the Study of Diabetes(EASD).Diabetologia 2018;61(12):2461-2498.
[3]Chaudhury A,Duvoor C,Dendi VSR,Kraleti S,Chada A,Ravilla R,et al.Clinical review of antidiabetic drugs:Implications for type 2 diabetes mellitus management.Front Endocrinol 2017;8:6.
[4]Aroda VR,Knowler WC,Crandall JP,Perreault L,Edelstein SL,Jeffries SL.Metformin for diabetes prevention:Insights gained from the Diabetes Prevention Program/Diabetes Prevention Program Outcomes Study.Diabetologia 2017;60(9):1601-1611.
[5]Palmer SC,Mavridis D,Nicolucci A,Johnson DW,Tonelli M,Craig JC.Comparison of clinical outcomes and adverse events associated with glucose-lowering drugs in patients with type 2 diabetes:A meta-analysis.JAMA 2016;316(3):313-324.
[6]Giovannini P,Howes MR,Edwards SE.Data on medicinal plants used in Central America to manage diabetes and its sequelae(skin conditions,cardiovascular disease,kidney disease,urinary problem and vision loss).Data Brief 2016;7:1217-1220.
[7]Boo CM,Ou-Yang CL,Omar-Hor K.1001 garden plants in Singapore.2nd ed.Singapore:National Parks Board;2006.
[8]Wahab SIA,Abdul AB,Mohan SM,Al-Zubairi AS,Elhassan MM,Ibrahim MY.Biological activities of Pereskia bleo extracts.Int JPharmacol 2009;5(1):71-75.
[9]Zareisedehizadeh S,Tan CH,Koh HL.A review of botanical characteristics,traditional usage,chemical components,pharmacological activities,and safety of Pereskia bleo(Kunth)DC.Evid Based Complement Alternat Med 2014;2014:326107.
[10]Sharf KM,Rahman MM,Zaidul ISM,Jannatul A,Akanda MJH,Mohamed A,et al.Pharmacological relevence of primitive leafy cactuses Pereskia.Res J Biotechnol 2013;8(12):134-142.
[11]Yen KP,Abdullah MS,Syafri S,Raju SK,Che Yahya CAH.Apreliminary survey on the medical uses and effectiveness of Pereskia bleo used by people of three villages in the State of Kelantan,Malaysia.Int JHerb Med 2013;1(3):1-4.
[12]Mat Darus NA,Mohamad J.Antidiabetic activity of Pereskia bleo aqueous extracts in alloxan induced diabetic rats.J Pharm Res 2017;1(7):000137.
[13]Nolte MS,Karam JH.Pancreatic hormones and antidiabetic drugs.In:Katzung BG(ed.)Basic and clinical pharmacology.8th ed.New York:Mc Graw-Hill;2001,p.721-723.
[14]Rena G,Hardie DG,Pearson ER.The mechanisms of action of metformin.Diabetologia 2017;60(9):1577-1585.
[15]Gilbert C,Valois M,Koren G.Pregnancy outcome after first-trimester exposure to metformin:A meta-analysis.Fertil Steril 2016;86(3):658-663.
[16]Bouchoucha M,Uzzan B,Cohen R.Metformin and digestive disorders.Diabetes Metab 2011;37(2):90-96.
[17]Duan B,Zhao Z,Lin L,Jin J,Zhang L,Xiong H,et al.Antidiabetic effect of Tibetan medicine Tang-Kang-Fu-San on high-fat diet and streptozotocin-induced type 2 diabetic rats.Evid Based Complement Alternat Med 2017;2017:7302965.
[18]Otah AA,Augustine O,Obiora IO,Maxwell E.Anthyperglycaemic effects of the methanol leaf extract of Diaphananthe bidens in normoglycemic and streptozotocin-induced hyperglycaemic rats.Asian Pac J Trop Med 2012;5(3):192-196.
[19]Ramachandran B,Ravi K,Narayanan V,Kandaswamy M,Subramanian S.Protective effect of macrocyclic binuclear oxovanadium complex on oxidative stress in pancreas of streptozotocin induced diabetic rats.Chem-Biol Interact 2004;149(1):9-21.
[20]Arunachalam K,Parimelazhagan.Antidiabetic activity of Ficus amplissima Smith.bark extract in streptozotocin induced diabetic rats.JEthnopharmacol 2013;147(2):302-310.
[21]Florence NT,Benoit MZ,Jonas K,Alexandra T,Desire DD,Pierre K,et al.Antidiabetic and antioxidant effects of Annona muricate(Annonaceae),aqueous extract on streptozotocin-induced diabetic rats.JEthnopharmacol 2014;151(2):784-790.
[22]Ali RB,Atangwho IJ,Kaur N,Mohamed EAH,Mohamed AJ,Asmawi MZ,et al.Hypoglycemic and anti-hyperglycemic study of Phaleria macrocarpa fruits pericarp.J Med Plants Res 2012;6(10):1982-1990.
[23]Shen Y,Fukushima M,Ito Y,Murak E,Hosono T,Seki T,et al.Verification of the antidiabetic effects of cinnamon(Cinnamomum zeylanicum)using insulin uncontrolled type 1 diabetic rats and cultured adipocytes.Biosci Biotechnol Biochem 2010;74:2418-2425.
[24]Juárez-Rojop IE,Díaz-Zagoya JC,Ble-Castillo JL,Miranda-Osorio PH,Castell-Rodríguez AE,Tovilla-Zárate CA,et al.Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats.BMCComplement Altern Med 2012;12:236-247.
[25]Al-Shamaony L,Al-Khazraji SM,Twaiji IIA.Hypoglycemic effect of Artemisia herba alba ll.Effect of a valuable extract on some blood parameters in diabetic animals.J Ethnopharmacol 1994;43(3):167-171.
[26]Adiga S,Bairy KL,Meharban A,Punita ISR.Hypoglycemic effect of aqueous extract of Trichosanthes dioica in normal and diabetic rats.Int JDiabetes Dev Ctries 2010;30(1):38-42.
[2]Davies MJ,Fradkin J,Kernan WN,Mathieu C,Mingrone G,Rossing P,et al.Management of hyperglycaemia in type 2 diabetes,2018.A consensus report by the American Diabetes Association(ADA)and the European Association for the Study of Diabetes(EASD).Diabetologia 2018;61(12):2461-2498.
[3]Chaudhury A,Duvoor C,Dendi VSR,Kraleti S,Chada A,Ravilla R,et al.Clinical review of antidiabetic drugs:Implications for type 2 diabetes mellitus management.Front Endocrinol 2017;8:6.
[4]Aroda VR,Knowler WC,Crandall JP,Perreault L,Edelstein SL,Jeffries SL.Metformin for diabetes prevention:Insights gained from the Diabetes Prevention Program/Diabetes Prevention Program Outcomes Study.Diabetologia 2017;60(9):1601-1611.
[5]Palmer SC,Mavridis D,Nicolucci A,Johnson DW,Tonelli M,Craig JC.Comparison of clinical outcomes and adverse events associated with glucose-lowering drugs in patients with type 2 diabetes:A meta-analysis.JAMA 2016;316(3):313-324.
[6]Giovannini P,Howes MR,Edwards SE.Data on medicinal plants used in Central America to manage diabetes and its sequelae(skin conditions,cardiovascular disease,kidney disease,urinary problem and vision loss).Data Brief 2016;7:1217-1220.
[7]Boo CM,Ou-Yang CL,Omar-Hor K.1001 garden plants in Singapore.2nd ed.Singapore:National Parks Board;2006.
[8]Wahab SIA,Abdul AB,Mohan SM,Al-Zubairi AS,Elhassan MM,Ibrahim MY.Biological activities of Pereskia bleo extracts.Int JPharmacol 2009;5(1):71-75.
[9]Zareisedehizadeh S,Tan CH,Koh HL.A review of botanical characteristics,traditional usage,chemical components,pharmacological activities,and safety of Pereskia bleo(Kunth)DC.Evid Based Complement Alternat Med 2014;2014:326107.
[10]Sharf KM,Rahman MM,Zaidul ISM,Jannatul A,Akanda MJH,Mohamed A,et al.Pharmacological relevence of primitive leafy cactuses Pereskia.Res J Biotechnol 2013;8(12):134-142.
[11]Yen KP,Abdullah MS,Syafri S,Raju SK,Che Yahya CAH.Apreliminary survey on the medical uses and effectiveness of Pereskia bleo used by people of three villages in the State of Kelantan,Malaysia.Int JHerb Med 2013;1(3):1-4.
[12]Mat Darus NA,Mohamad J.Antidiabetic activity of Pereskia bleo aqueous extracts in alloxan induced diabetic rats.J Pharm Res 2017;1(7):000137.
[13]Nolte MS,Karam JH.Pancreatic hormones and antidiabetic drugs.In:Katzung BG(ed.)Basic and clinical pharmacology.8th ed.New York:Mc Graw-Hill;2001,p.721-723.
[14]Rena G,Hardie DG,Pearson ER.The mechanisms of action of metformin.Diabetologia 2017;60(9):1577-1585.
[15]Gilbert C,Valois M,Koren G.Pregnancy outcome after first-trimester exposure to metformin:A meta-analysis.Fertil Steril 2016;86(3):658-663.
[16]Bouchoucha M,Uzzan B,Cohen R.Metformin and digestive disorders.Diabetes Metab 2011;37(2):90-96.
[17]Duan B,Zhao Z,Lin L,Jin J,Zhang L,Xiong H,et al.Antidiabetic effect of Tibetan medicine Tang-Kang-Fu-San on high-fat diet and streptozotocin-induced type 2 diabetic rats.Evid Based Complement Alternat Med 2017;2017:7302965.
[18]Otah AA,Augustine O,Obiora IO,Maxwell E.Anthyperglycaemic effects of the methanol leaf extract of Diaphananthe bidens in normoglycemic and streptozotocin-induced hyperglycaemic rats.Asian Pac J Trop Med 2012;5(3):192-196.
[19]Ramachandran B,Ravi K,Narayanan V,Kandaswamy M,Subramanian S.Protective effect of macrocyclic binuclear oxovanadium complex on oxidative stress in pancreas of streptozotocin induced diabetic rats.Chem-Biol Interact 2004;149(1):9-21.
[20]Arunachalam K,Parimelazhagan.Antidiabetic activity of Ficus amplissima Smith.bark extract in streptozotocin induced diabetic rats.JEthnopharmacol 2013;147(2):302-310.
[21]Florence NT,Benoit MZ,Jonas K,Alexandra T,Desire DD,Pierre K,et al.Antidiabetic and antioxidant effects of Annona muricate(Annonaceae),aqueous extract on streptozotocin-induced diabetic rats.JEthnopharmacol 2014;151(2):784-790.
[22]Ali RB,Atangwho IJ,Kaur N,Mohamed EAH,Mohamed AJ,Asmawi MZ,et al.Hypoglycemic and anti-hyperglycemic study of Phaleria macrocarpa fruits pericarp.J Med Plants Res 2012;6(10):1982-1990.
[23]Shen Y,Fukushima M,Ito Y,Murak E,Hosono T,Seki T,et al.Verification of the antidiabetic effects of cinnamon(Cinnamomum zeylanicum)using insulin uncontrolled type 1 diabetic rats and cultured adipocytes.Biosci Biotechnol Biochem 2010;74:2418-2425.
[24]Juárez-Rojop IE,Díaz-Zagoya JC,Ble-Castillo JL,Miranda-Osorio PH,Castell-Rodríguez AE,Tovilla-Zárate CA,et al.Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats.BMCComplement Altern Med 2012;12:236-247.
[25]Al-Shamaony L,Al-Khazraji SM,Twaiji IIA.Hypoglycemic effect of Artemisia herba alba ll.Effect of a valuable extract on some blood parameters in diabetic animals.J Ethnopharmacol 1994;43(3):167-171.
[26]Adiga S,Bairy KL,Meharban A,Punita ISR.Hypoglycemic effect of aqueous extract of Trichosanthes dioica in normal and diabetic rats.Int JDiabetes Dev Ctries 2010;30(1):38-42.