多发性骨髓瘤患者血清中可溶性NKG2D配体表达及临床意义
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摘要
目的探讨多发性骨髓瘤(Multiple Myeloma,MM)患者血清中可溶性NKG2D配体的表达水平以及其临床意义。方法采用双抗体酶联免疫吸附实验法(Enzyme linked immunosorbent assay,ELISA)定量检测32例MM患者和30例健康人血清中可溶性NKG2D配体表达水平,并用流式细胞仪检测13例MM患者表面MICA/B的表达水平,并对检测结果进行统计学分析。结果 MM患者血清中可溶性NKG2D配体包括sMICA和sMICB的水平明显高于健康对照组,P<0.01。Ⅲ期的MM患者血清中sMICA和sMICB的水平明显高于Ⅰ期和Ⅱ期的患者,差异有统计学意义,P<0.05。在13例MM患者中血清中sMICA和sMICB的水平与患者表面MICA/B表达水平并没有直接相关性。结论 MM患者血清中可溶性NKG2D的配体水平增高与临床分期有关,可作为MM患者病情的进展和预后间接预测因子。
OBJECTIVE To explore the change and clinical significances of serum soluble NKG2 Dligands in patients with multiple myeloma.METHODS Detected the levels of soluble NKG2Dligands(MICA and MICB)in serum by enzyme-linked immunosorbent assay(ELISA);Checked the expression of NKG2Dligands(MICA/B)on MM cells by flow cytometry.RESULTS The MM patients had higher serum levels of sMICA and sMICB than the healthy individual groups(P<0.05).Serum levels of sMICA and sMICB were highest in MM patients with clinical stage Ⅲ,compared with stageⅠand Ⅱ.Serum levels of sMICA and sMICB were significantly decreased by chemotherapy.Soluble sMICA and sMICB have no correlation with member-type MICA/B expression on MM cells of patient's bone marrow in the 13 detected patients.CONCLUSION Serum levels of sMICA and sMICB were increased in patients with MM,and was related with MM clinical stage,can be taken as the predictive factors for progression and prognosis of patients with MM.
OBJECTIVE To explore the change and clinical significances of serum soluble NKG2 Dligands in patients with multiple myeloma.METHODS Detected the levels of soluble NKG2Dligands(MICA and MICB)in serum by enzyme-linked immunosorbent assay(ELISA);Checked the expression of NKG2Dligands(MICA/B)on MM cells by flow cytometry.RESULTS The MM patients had higher serum levels of sMICA and sMICB than the healthy individual groups(P<0.05).Serum levels of sMICA and sMICB were highest in MM patients with clinical stage Ⅲ,compared with stageⅠand Ⅱ.Serum levels of sMICA and sMICB were significantly decreased by chemotherapy.Soluble sMICA and sMICB have no correlation with member-type MICA/B expression on MM cells of patient's bone marrow in the 13 detected patients.CONCLUSION Serum levels of sMICA and sMICB were increased in patients with MM,and was related with MM clinical stage,can be taken as the predictive factors for progression and prognosis of patients with MM.
引文
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