MST1和MST2在胃癌中的表达及意义
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摘要
目的:探讨MST1、MST2在胃癌组织中的表达及意义。方法:采用SP免疫组化方法检测MST1、MST2在70例非贲门胃癌组织及70例远端切缘的表达情况,分析MST1、MST2的表达水平与胃癌的关系。结果:MST1在胃癌组织中的表达明显低于远端切缘的表达,其表达与胃癌的分化程度、淋巴结转移、肿瘤分期、肿瘤大小关联;MST2在胃癌组织中的表达水平明显低于远端切缘的表达,其表达与胃癌的分化程度、淋巴结转移、肿瘤分期关联。结论:MST1和MST2可能参与了胃癌的发生发展。
Objective:To investigate the expressions and significances of MST1 and MST2 in gastric cancer tissues. Methods:Using SP immunohistochemical method,the protein expression levels of MST1 and MST2 were detected in 70 tumor tissues and 70 normal tissues obtained from the same patients with non-cardia gastric cancer. The relationship between the expression level of MST1 or MST2 and the development of gastric cancer was analyzed. Results:The expression of MST1 in gastric cancer tissues was significantly lower than in the controls. The expression was related to the degree of differentiation of gastric cancer,lymph node metastasis,neoplasms staging and tumor size. The expression of MST2 in gastric cancer tissue was also significantly lower than in the controls. The expression was related to the degree of differentiation of gastric cancer,lymph node metastasis,and neoplasms staging. Conclusion:MST1 and MST2 may play an important role in the development of gastric cancer.
Objective:To investigate the expressions and significances of MST1 and MST2 in gastric cancer tissues. Methods:Using SP immunohistochemical method,the protein expression levels of MST1 and MST2 were detected in 70 tumor tissues and 70 normal tissues obtained from the same patients with non-cardia gastric cancer. The relationship between the expression level of MST1 or MST2 and the development of gastric cancer was analyzed. Results:The expression of MST1 in gastric cancer tissues was significantly lower than in the controls. The expression was related to the degree of differentiation of gastric cancer,lymph node metastasis,neoplasms staging and tumor size. The expression of MST2 in gastric cancer tissue was also significantly lower than in the controls. The expression was related to the degree of differentiation of gastric cancer,lymph node metastasis,and neoplasms staging. Conclusion:MST1 and MST2 may play an important role in the development of gastric cancer.
引文
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[21]Artemenko Y,Batsios P,Borleis J,et al.Tumor suppressor Hippo/MST1 ki-nase mediates chemotaxis by regulating spreading and adhesion[J].Proc Natl Acad Sci USA,2012,109:13632-13637.
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[23]Song H,Mak KK,Topol L,et al.Mammalian MST1 and MST2 kinases play essential roles in organ size control and tumor suppression[J].Proc Natl Acad Sci USA,2010,107(4):1431-1436.
[24]Barry ER,Camargo FD.The Hippo superhighway:Signaling crossroads converging on the Hippo/Yap pathway in stem cells and development[J].Curr Opin Cell Biol,2013,25(2):247-253.
[25]Zhou Zhou,Zhu Jinshui.Research advances on Hippo signal pathway and YAP gene[J].Modern Oncology,2011,19(06):1241-1244.[周洲,朱金水.Hippo通路及YAP基因研究的新进展[J].现代肿瘤医学,2011,19(06):1241-1244.]
[26]Xu ZP,Zhu JS,Zhang Q,et al.A breakdown of the Hippo pathway in gastric cancer[J].Hepatogastroenterology,2011,58(110-111):1611-1617.
[27]Wei WC.The role of Hippo signaling pathway in gastric mucosal carcinogenesis[D].Qingdao:Qingdao university,2013.[魏文超.Hippo信号通路在胃黏膜癌变过程中的作用[D].青岛:青岛大学,2013.]
[2]Zou XN,Sun X,Chen WQ,et al.Analysis of the incidence and death of gastric cancer in China from 2003 to 2007[J].Tumor,2012,32(2):109-114.[邹小农,孙喜,陈万青,等.2003-2007年中国胃癌发病与死亡情况分析[J].肿瘤,2012,32(2):109-114.]
[3]Lin Y,Ueda J,kikuchi S,et al.Comparative epidemiology of gastric cancer between Japan and China[J].Word J Gastroenterol,2011,17(39):4421-4428.
[4]Jarosz M,Sekula W,Rychlik E,et al.Impact of diet on long-term decline in gastric cancer incidence in Poland[J].World Journal of Gastroenterology,2011,17(01):89-97.
[5]Shi ZH,Liu H.The infection of Helicobacter pylori and gastric cancer[J].World Chinese Digest Magazine,2011,19(32):3327-3331.[时昭红,刘浩.幽门螺杆菌感染与胃癌[J].世界华人消化杂志,2011,19(32):3327-3331.]
[6]Hilman D,Gat U.The evolutionary history of YAP and The Hippo/YAP pathway[J].Mol Biol Evol,2011,28(8):2403-2417.
[7]Zeng Q,Hong W.The emerging role of the hippo pathway in cell contact inhibition,organ size control,and cancer development in mammals[J].Cancer Cell,2008,13(3):188-192.
[8]Halder G,Johnson RL.Hippo signaling:Growth control and beyond[J].Development,2011,138(1):9-22.
[9]Yu FX,Guan KL.The Hippo pathway:Regulators and regulation[J].Genes Dev,2013,27(4):355-371.
[10]Hu Liqiao,Zhou Zhaocai,Tian Wei.Research progresses on the molecular structure of the Hippo signaling pathway components[J].Heredity,2017,39(07):659-674.[胡立桥,周兆才,田伟.Hippo信号通路结构生物学研究进展[J].遗传,2017,39(07):659-674.]
[11]Mo JS,Park HW,Guan KL.The Hippo signaling pathway in stem cell biology and cancer[J].EMBO Rep,2014,15(6):642-656.
[12]Yin MX,Zhang L.Hippo signaling in epithelial stem cells[J].Acta Biochim Biophys Sin(Shanghai),2015,47(1):39-45.
[13]Ramos A,Camargo FD.The Hippo signaling pathway and stem cell biology[J].Trends Cell Biol,2012,22(7):339-346.
[14]Wei Kang,Alfred SL Cheng,Jun Yu,et al.Emerging role of Hippo pathway in gastric and other gastrointestinal cancers[J].World Journal of Gastroenterology,2016,22(03):1279-1288.
[15]Harvey KF,Zhang X,Thomas DM.The Hippo pathway and human cancer[J].Nat Rev Cancer,2013,13(4):246-257.
[16]Yin M,Zhang L.Hippo signaling:A hub of growth control,tumor suppression and pluripotency maintenance[J].J Genet Genomics,2011,38(10):471-481.
[17]Assouline S,Culjkovic B,Cocolakis E,et al.Molecular targeting of the oncogene e IF4E in acute myeloid leukemia(AML):A proof-of-principle clinical trial with ribavirin[J].Blood,2009,114(2):257-260.
[18]Zheng ZH,Zhang ZG,Wu HJ,et al.Hippo signaling pathway in kidney diseases[J].Clinical and Pathology,2017,37(07):1518-1523.[郑志煌,张志刚,吴慧娟,等.Hippo信号通路与肾病[J].临床与病理杂志,2017,37(07):1518-1523.]
[19]Liu YC,Xing Y,Cai L.Role of hippo signaling pathway in lung cancer.[J].China Lung Cancer,2017,20(09):629-634.[刘悦超,邢影,蔡莉.Hippo信号通路在肺癌中的研究进展[J].中国肺癌杂志,2017,20(09):629-634.]
[20]Meng Z,Moroishi T,Mottier Pavie V,et al.MAP4K family kinases act in parallel to MST1/2 to activate LATS1/2 in the Hippo pathway[J].Nat Commun,2015,6:8357-8357.
[21]Artemenko Y,Batsios P,Borleis J,et al.Tumor suppressor Hippo/MST1 ki-nase mediates chemotaxis by regulating spreading and adhesion[J].Proc Natl Acad Sci USA,2012,109:13632-13637.
[22]Zhou JC,Tang SS.Research progress in biological function and signal regulation of MST1[J].Clinical and Pathology,2016,36(02):209-214.[周杰灿,唐圣松.MST1生物学作用及信号调控研究进展[J].临床与病理杂志,2016,36(02):209-214.]
[23]Song H,Mak KK,Topol L,et al.Mammalian MST1 and MST2 kinases play essential roles in organ size control and tumor suppression[J].Proc Natl Acad Sci USA,2010,107(4):1431-1436.
[24]Barry ER,Camargo FD.The Hippo superhighway:Signaling crossroads converging on the Hippo/Yap pathway in stem cells and development[J].Curr Opin Cell Biol,2013,25(2):247-253.
[25]Zhou Zhou,Zhu Jinshui.Research advances on Hippo signal pathway and YAP gene[J].Modern Oncology,2011,19(06):1241-1244.[周洲,朱金水.Hippo通路及YAP基因研究的新进展[J].现代肿瘤医学,2011,19(06):1241-1244.]
[26]Xu ZP,Zhu JS,Zhang Q,et al.A breakdown of the Hippo pathway in gastric cancer[J].Hepatogastroenterology,2011,58(110-111):1611-1617.
[27]Wei WC.The role of Hippo signaling pathway in gastric mucosal carcinogenesis[D].Qingdao:Qingdao university,2013.[魏文超.Hippo信号通路在胃黏膜癌变过程中的作用[D].青岛:青岛大学,2013.]