探究乌司他丁和多西他赛对人乳腺癌细胞MDA-MB-231增殖、侵袭的影响
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摘要
目的探究分析乌司他丁和多西他赛对体外培养的人乳腺癌细胞MDA-MB-231增殖、侵袭和迁移能力的影响。方法将人乳腺癌细胞MDA-MB-231随机分为乌司他丁组、多西他赛组、乌司他丁联合多西他赛组以及对照组四个组,分别予以800 U/ml乌司他丁、3. 7μg/ml多西他赛、800 U/ml乌司他丁+3. 7μg/ml多西他赛以及等量生理盐水干预。分别在干预后0 h、24 h、48 h、72 h时点采用CCK8法检测MDA-MB-231的增殖能力,在干预后48 h采用Transwell法检测MDA-MB-231的侵袭、迁移能力。结果与对照组相比,乌司他丁组、多西他赛组、乌司他丁联合多西他赛组24 h、48 h、72 h等各时点MDA-MB-231的增殖能力均明显降低(P <0. 05),且多西他赛组的增殖能力较同时点乌司他丁组较低(P <0. 05),乌司他丁联合多西他赛组各时点增殖能力则明显降低(P <0. 05),组间比较差异明显,具有统计学意义。与对照组相比,乌司他丁组、多西他赛组、乌司他丁联合多西他赛组各组MDA-MB-231细胞侵袭、迁移能力明显降低(P <0. 05),且乌司他丁联合多西他赛组细胞侵袭、迁移能力明显低于多西他赛组及乌司他丁组水平(P <0. 05),多西他赛组细胞侵袭、迁移能力明显低于乌司他丁组(P <0. 05),组间差异具有显著统计学意义。结论乌司他丁与多西他赛均可在一定程度上抑制人乳腺癌细胞MDA-MB-231的增殖、侵袭和迁移,且乌司他丁和多西他赛联合应用抑制效果优势明显。
Objective To investigate and analyze the effect of ulinastatin and docetaxel on proliferation,invasion and migration of MDA-MB-231 human breast cancer cells. Methods Human breast cancer cells MDA-MB-231 were randomly divided into four groups: ulinastatin group,docetaxel group,ulinastatin combined with docetaxel group and control group,with intervention of 800 U/ml ulinastatin,3. 7 μg/ml docetaxel,800 U/ml ulinastatin + 3. 7 μg/ml docetaxel and same amount of normal saline,respectively. Proliferation ability of MDA-MB-231 was detected by CCK8 method at 0 h,24 h,48 h and 72 h after intervention. Invasion and migration ability of MDA-MB-231 was detected by Transwell method 48 h after intervention. Results Compared with control group,the other three groups had lower proliferation at each timepoints( P < 0. 05); proliferation of docetaxel group was lower at same time points compared with ulinastatin group( P < 0. 05),and combination group had significantly lower proliferation ability( P < 0. 05). Compared with control group,the other three groups had significantly lower invasion and migration ability( P < 0. 05). Combination group had even lower invasion and migration ability( P < 0. 05),and docetaxel group had lower cell invasion and migration ability than ulinastatin group( P < 0. 05). Conclusion Both ulinastatin and docetaxel can inhibit proliferation,invasion and migration of MDA-MB-231 human breast cancer cells to some extent.
Objective To investigate and analyze the effect of ulinastatin and docetaxel on proliferation,invasion and migration of MDA-MB-231 human breast cancer cells. Methods Human breast cancer cells MDA-MB-231 were randomly divided into four groups: ulinastatin group,docetaxel group,ulinastatin combined with docetaxel group and control group,with intervention of 800 U/ml ulinastatin,3. 7 μg/ml docetaxel,800 U/ml ulinastatin + 3. 7 μg/ml docetaxel and same amount of normal saline,respectively. Proliferation ability of MDA-MB-231 was detected by CCK8 method at 0 h,24 h,48 h and 72 h after intervention. Invasion and migration ability of MDA-MB-231 was detected by Transwell method 48 h after intervention. Results Compared with control group,the other three groups had lower proliferation at each timepoints( P < 0. 05); proliferation of docetaxel group was lower at same time points compared with ulinastatin group( P < 0. 05),and combination group had significantly lower proliferation ability( P < 0. 05). Compared with control group,the other three groups had significantly lower invasion and migration ability( P < 0. 05). Combination group had even lower invasion and migration ability( P < 0. 05),and docetaxel group had lower cell invasion and migration ability than ulinastatin group( P < 0. 05). Conclusion Both ulinastatin and docetaxel can inhibit proliferation,invasion and migration of MDA-MB-231 human breast cancer cells to some extent.
引文
[1]陈健文,张仁伟,温炜杰,等.国产乌司他丁对丝氨酸蛋白酶抑制效果的体外研究[J].中国生化药物杂志,2015,35(8):15-18.
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[7]薛芳沁,杨国华,林孟波,等. PS-341对人乳腺癌MDA-MB231细胞uPA、NF-κB表达的影响及其与增殖迁移的关系[J].福建医药杂志,2015,37(4):5-8.
[8]胡祖健,徐海滨,任兴昌,等.乳腺癌转移抑制基因、尿激酶型纤溶酶原激活剂在乳腺癌组织中的表达[J].全科医学临床与教育,2015,13(2):132-134.
[9]刘志聪,王斌,华锋,等.乌司他丁对急性肺损伤患者血清纤溶酶原激活物抑制剂-1、组织型纤溶酶原激活剂的影响[J].中国医药导报,2016,13(24):148-151.
[10]张利鹏,赵焱,刘国娟,等.乌司他丁通过抑制ERK信号通路减轻大鼠脓毒症性肺损伤[J].临床和实验医学杂志,2017,16(1):8-11.
[11]周东民,贾启明,黄静,等.乌司他丁预先给药对大鼠呼吸机相关性肺损伤时p-ERK表达的影响[J].中华麻醉学杂志,2016,36(8):1015-1018.
[12]张利鹏,赵焱,刘国娟,等.乌司他丁通过干预p38MAPK/ERK信号通路减轻脓毒症性肺损伤[J].中国药理学通报,2016,32(9):1311-1316.
[13]王焰斌,崔刚,王小雷,等. Fas/Fas L信号通路在乌司他丁后处理减轻CPB下心脏瓣膜置换术患者心肌细胞凋亡中的作用[J].中华麻醉学杂志,2014,34(8):940-943.
[14]孟婷婷,李井泉,齐宪荣.可共载基因与敏化剂用以增强多西他赛抗肿瘤效果的脂质纳米载药系统的制备与评价[J].中国药学(英文版),2014,23(3):145-152.
[15]钟彪,孙治君,付裕,等.乌司他丁和泰索帝对人原代乳腺癌细胞增殖、侵袭、凋亡的影响及其机制[J].中国生物制品学杂志,2011,24(10):1197-1202.
[2]尚春迎.乳腺癌术前TEC辅助化疗的疗效及其影响因素[J].实用癌症杂志,2015,30(11):1684-1686.
[3]白玉盘,杨小利,欧周罗. m TOR信号通路介导产生XCL1可促进乳腺癌耐药细胞株的增殖[J].中国癌症杂志,2014,24(10):770-776.
[4]李明,田甜,吴凤霞,等.三维培养下脂肪细胞对乳腺癌MCF-7细胞迁移影响及其机制的初步研究[J].中华肿瘤防治杂志,2016,23(10):631-635.
[5]石建伟,唐智柳,蔡美玉,等. 2008~2012年我国女性乳腺癌流行状况的系统性综述[J].中国妇幼保健,2014,29(10):1622-1626.
[6]崔玉洁,张凯,苏同义,等.乳腺癌分子靶向治疗研究进展[J].解放军医药杂志,2014,26(1):95-98.
[7]薛芳沁,杨国华,林孟波,等. PS-341对人乳腺癌MDA-MB231细胞uPA、NF-κB表达的影响及其与增殖迁移的关系[J].福建医药杂志,2015,37(4):5-8.
[8]胡祖健,徐海滨,任兴昌,等.乳腺癌转移抑制基因、尿激酶型纤溶酶原激活剂在乳腺癌组织中的表达[J].全科医学临床与教育,2015,13(2):132-134.
[9]刘志聪,王斌,华锋,等.乌司他丁对急性肺损伤患者血清纤溶酶原激活物抑制剂-1、组织型纤溶酶原激活剂的影响[J].中国医药导报,2016,13(24):148-151.
[10]张利鹏,赵焱,刘国娟,等.乌司他丁通过抑制ERK信号通路减轻大鼠脓毒症性肺损伤[J].临床和实验医学杂志,2017,16(1):8-11.
[11]周东民,贾启明,黄静,等.乌司他丁预先给药对大鼠呼吸机相关性肺损伤时p-ERK表达的影响[J].中华麻醉学杂志,2016,36(8):1015-1018.
[12]张利鹏,赵焱,刘国娟,等.乌司他丁通过干预p38MAPK/ERK信号通路减轻脓毒症性肺损伤[J].中国药理学通报,2016,32(9):1311-1316.
[13]王焰斌,崔刚,王小雷,等. Fas/Fas L信号通路在乌司他丁后处理减轻CPB下心脏瓣膜置换术患者心肌细胞凋亡中的作用[J].中华麻醉学杂志,2014,34(8):940-943.
[14]孟婷婷,李井泉,齐宪荣.可共载基因与敏化剂用以增强多西他赛抗肿瘤效果的脂质纳米载药系统的制备与评价[J].中国药学(英文版),2014,23(3):145-152.
[15]钟彪,孙治君,付裕,等.乌司他丁和泰索帝对人原代乳腺癌细胞增殖、侵袭、凋亡的影响及其机制[J].中国生物制品学杂志,2011,24(10):1197-1202.
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