广西莪术水提物对卵形鲳鲹细胞免疫力的影响
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摘要
卵形鲳鲹(Trachinotus ovatus)是我国重要的海水养殖鱼类,经济价值极高,但近年来各种鱼病频繁爆发造成了巨大损失。为探究广西莪术(Curcuma kwangsiensis S.G.Lee et C.F.Liang)水提物对卵形鲳鲹免疫力的影响,本文在细胞水平上,利用卵形鲳鲹肾脏组织细胞系研究广西莪术水提物的细胞毒性,以确定其细胞安全浓度。然后利用卵形鲳鲹细胞系研究广西莪术水提物对总一氧化氮合酶(T-NOS)、酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)和溶菌酶等卵形鲳鲹细胞免疫相关酶的影响。结果表明,广西莪术水提物的细胞安全浓度为≤5mg/mL,广西莪术水提物对卵形鲳鲹细胞的免疫相关酶活力(总一氧化氮合酶、酸性磷酸酶、超氧化物歧化酶、溶菌酶)有明显促进作用。广西莪术有潜力发展成为一种高效的水产免疫增强剂,并且基于传统壮药积极开展绿色无药物残留的新型抗水产病害中药制剂的研究,对于促进水产养殖业持续健康发展,确保水产品质量和食品安全意义重大。
Trachinotus ovatus is an important marine fish with high economic value in China.However,the frequent outbreak of various fish diseases has caused great losses in recent years.In order to investigate the effects of Curcuma kwangsiensis S.G.Lee et C.F.Liang on the immunity of To.vatus,at the cellular level,the cytotoxicity of water extracts of Curcuma kwangsiensis S.G.Lee et C.F.Liang in Guangxi was studied using the ovate-shaped kidney tissue cell line to determine the safe concentration of cells.Then,the effect of water extracts of Curcuma kwangsiensis S.G.Lee et C.F.Liang on the immune-related enzymes of ovate-shaped cells which included total nitrogen monoxide synthase(T-NOS),acid phosphatase(ACP),superoxide dismutase(SOD)and lysozyme was studied by using the ovate cell line.The safe concentration of Curcuma kwangsiensis S.G.Lee et C.F.Liang water extracts was≤5 mg/mL.Curcuma kwangsiensis S.G.Lee et C.F.Liang could obviously promote the activity of immune-related enzymes(T-NOS,ACP,SOD and lysozyme)in TOK cells.CurcumakwangsiensisS.G.Lee et C.F.Liang has potential to develop into a highly effective aquaculture immune enhancer.Based on the traditional Chinese Zhuang medicine,it is of great significance to carry out the research on new Chinese herbal preparations against aquatic diseases,which could promote the sustainable and healthy development of aquaculture and ensure the quality of aquatic products and food safety.
Trachinotus ovatus is an important marine fish with high economic value in China.However,the frequent outbreak of various fish diseases has caused great losses in recent years.In order to investigate the effects of Curcuma kwangsiensis S.G.Lee et C.F.Liang on the immunity of To.vatus,at the cellular level,the cytotoxicity of water extracts of Curcuma kwangsiensis S.G.Lee et C.F.Liang in Guangxi was studied using the ovate-shaped kidney tissue cell line to determine the safe concentration of cells.Then,the effect of water extracts of Curcuma kwangsiensis S.G.Lee et C.F.Liang on the immune-related enzymes of ovate-shaped cells which included total nitrogen monoxide synthase(T-NOS),acid phosphatase(ACP),superoxide dismutase(SOD)and lysozyme was studied by using the ovate cell line.The safe concentration of Curcuma kwangsiensis S.G.Lee et C.F.Liang water extracts was≤5 mg/mL.Curcuma kwangsiensis S.G.Lee et C.F.Liang could obviously promote the activity of immune-related enzymes(T-NOS,ACP,SOD and lysozyme)in TOK cells.CurcumakwangsiensisS.G.Lee et C.F.Liang has potential to develop into a highly effective aquaculture immune enhancer.Based on the traditional Chinese Zhuang medicine,it is of great significance to carry out the research on new Chinese herbal preparations against aquatic diseases,which could promote the sustainable and healthy development of aquaculture and ensure the quality of aquatic products and food safety.
引文
[1]李坚明.“十三五”广西水产养殖业发展战略研究[J].中国渔业经济,2016,34(4):25-31.
[2]余庆,李菲,王一兵,等.广西北部湾大宗海水养殖鱼类卵形鲳鲹感染溶藻弧菌及其致病性的研究[J].广西科学,2018,25(1):68-73.
[3]余庆,李菲,覃仙玲,等.广西卵形鲳鲹小脑来源细胞系的建立及特征分析[J].广西科学,2018,25(1):74-79.
[4]李鹏飞,余庆,覃仙玲,等.广西北部湾海水养殖业现状与病害防控技术体系研究展望[J].广西科学,2018,25(1):15-25.
[5]WANG X,RYU D,HOUTKOOPER R H,et al.Antibiotic use and abuse:A threat to mitochondria and chloroplasts with impact on research,health,and environment[J].Bioessays,2015,37(10):1045-1053.DOI:10.1002/bies.201500071.
[6]SMALDONE G,MARRONE R,CAPPIELLO S,et al.Occurrence of antibiotic resistance in bacteria isolated from seawater organisms caught in Campania Region:Preliminary study[J].BMC Veterinary Research,2014,10:161.
[7]陈昌福.我国水产养殖动物病害防治研究的主要成就与当前存在的问题[J].饲料工业,2007,28(10):1-3.
[8]张贵杰,黄克斌.广西莪术化学成分和药理作用研究进展[J].广州化工,2015,11:24-26.
[9]ZHOU L L,LI P F,LIU J X,et al.Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus,a new cell model for virus pathogenesis and toxicology studies[J].In Vitro Cellular&Developmental Biology-Animal,2017,53(4):320-327.
[10]朱凯,李军,罗桓,等.广西莪术化学成分的分离与鉴定[J].沈阳药科大学学报,2009,26(1):27-29.
[11]ZENG J,DAI P,REN L,et al.Apoptosis-induced antitumor effect of Curcuma kwangsiensis polysaccharides against human nasopharyngeal carcinoma cells[J].Carbohydr Polym,2012,89(4):1067-1072.DOI:10.1016/j.carbpol.2012.03.064.
[12]彭岳,吴光,韦燕飞,等.桂郁金提取物对人肝星状细胞增殖、细胞周期及凋亡影响的研究[J].时珍国医国药,2012,23(5):1076-1078.
[13]LI P F,ZHOU L L,NI S W,et al.Establishment and characterization of a novel cell line from the brain of golden pompano(Trachinotus ovatus)[J].In Vitro Cellular&Developmental Biology-Animal,2016,52(4):410-418.
[14]LI P F,ZHOU L L,WEI S N,et al.Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility[J].Journal of Fish Biology,2017,90(5):1944-1959.DOI:10.1111/jfb.13277.
[15]LI P F,ZHOU L L,WEI J G,et al.Development and characterization of aptamer-based enzyme-linked aptasorbent assay for the detection of Singapore grouper iridovirus infection[J].Journal of Applied Microbiology,2016,121(3):634-643.DOI:10.1111/jam.13161.
[16]ZHOU L L,LI P F,NI S W,et al.Rapid and sensitive detection of redspotted grouper nervous necrosis virus(RGNNV)infection by aptamer-coat protein-aptamer sandwich enzyme-linked apta-sorbent assay(ELASA)[J].Journal of Fish Diseases,2017,40(12):1831-1838.DOI:10.1111/jfd.12656.
[17]LI P F,ZHOU L L,YU Y P,et al.Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects[J].BMC Veterinary Research,2015,11(1):245.DOI:10.1186/s12917-015-0559-6.
[18]LI P F,WEI S N,ZHOU L L,et al.Selection and characterization of novel specific DNA aptamers recognized by fish cells infected with Singapore grouper iridovirus[J].The Journal of General Virology,2015,96:3348-3359.
[19]YU Q,LIU M Z,WEI S N,et al.Isolation and characterization of nervous necrosis virus in cultured hybrid grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂)in Guangxi,China[J].Fish Pathology,2019,54(1):16-19.
[20]YU Q,LIU M Z,XIAO H H,et al.The inhibitory activities and antiviral mechanism of Viola philippica aqueous extracts against grouper iridovirus infection in vitro and in vivo[J].Journal of Fish Diseases,2019.DOI:10.1111/jfd.12987.[Epub ahead of print]
[2]余庆,李菲,王一兵,等.广西北部湾大宗海水养殖鱼类卵形鲳鲹感染溶藻弧菌及其致病性的研究[J].广西科学,2018,25(1):68-73.
[3]余庆,李菲,覃仙玲,等.广西卵形鲳鲹小脑来源细胞系的建立及特征分析[J].广西科学,2018,25(1):74-79.
[4]李鹏飞,余庆,覃仙玲,等.广西北部湾海水养殖业现状与病害防控技术体系研究展望[J].广西科学,2018,25(1):15-25.
[5]WANG X,RYU D,HOUTKOOPER R H,et al.Antibiotic use and abuse:A threat to mitochondria and chloroplasts with impact on research,health,and environment[J].Bioessays,2015,37(10):1045-1053.DOI:10.1002/bies.201500071.
[6]SMALDONE G,MARRONE R,CAPPIELLO S,et al.Occurrence of antibiotic resistance in bacteria isolated from seawater organisms caught in Campania Region:Preliminary study[J].BMC Veterinary Research,2014,10:161.
[7]陈昌福.我国水产养殖动物病害防治研究的主要成就与当前存在的问题[J].饲料工业,2007,28(10):1-3.
[8]张贵杰,黄克斌.广西莪术化学成分和药理作用研究进展[J].广州化工,2015,11:24-26.
[9]ZHOU L L,LI P F,LIU J X,et al.Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus,a new cell model for virus pathogenesis and toxicology studies[J].In Vitro Cellular&Developmental Biology-Animal,2017,53(4):320-327.
[10]朱凯,李军,罗桓,等.广西莪术化学成分的分离与鉴定[J].沈阳药科大学学报,2009,26(1):27-29.
[11]ZENG J,DAI P,REN L,et al.Apoptosis-induced antitumor effect of Curcuma kwangsiensis polysaccharides against human nasopharyngeal carcinoma cells[J].Carbohydr Polym,2012,89(4):1067-1072.DOI:10.1016/j.carbpol.2012.03.064.
[12]彭岳,吴光,韦燕飞,等.桂郁金提取物对人肝星状细胞增殖、细胞周期及凋亡影响的研究[J].时珍国医国药,2012,23(5):1076-1078.
[13]LI P F,ZHOU L L,NI S W,et al.Establishment and characterization of a novel cell line from the brain of golden pompano(Trachinotus ovatus)[J].In Vitro Cellular&Developmental Biology-Animal,2016,52(4):410-418.
[14]LI P F,ZHOU L L,WEI S N,et al.Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility[J].Journal of Fish Biology,2017,90(5):1944-1959.DOI:10.1111/jfb.13277.
[15]LI P F,ZHOU L L,WEI J G,et al.Development and characterization of aptamer-based enzyme-linked aptasorbent assay for the detection of Singapore grouper iridovirus infection[J].Journal of Applied Microbiology,2016,121(3):634-643.DOI:10.1111/jam.13161.
[16]ZHOU L L,LI P F,NI S W,et al.Rapid and sensitive detection of redspotted grouper nervous necrosis virus(RGNNV)infection by aptamer-coat protein-aptamer sandwich enzyme-linked apta-sorbent assay(ELASA)[J].Journal of Fish Diseases,2017,40(12):1831-1838.DOI:10.1111/jfd.12656.
[17]LI P F,ZHOU L L,YU Y P,et al.Characterization of DNA aptamers generated against the soft-shelled turtle iridovirus with antiviral effects[J].BMC Veterinary Research,2015,11(1):245.DOI:10.1186/s12917-015-0559-6.
[18]LI P F,WEI S N,ZHOU L L,et al.Selection and characterization of novel specific DNA aptamers recognized by fish cells infected with Singapore grouper iridovirus[J].The Journal of General Virology,2015,96:3348-3359.
[19]YU Q,LIU M Z,WEI S N,et al.Isolation and characterization of nervous necrosis virus in cultured hybrid grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂)in Guangxi,China[J].Fish Pathology,2019,54(1):16-19.
[20]YU Q,LIU M Z,XIAO H H,et al.The inhibitory activities and antiviral mechanism of Viola philippica aqueous extracts against grouper iridovirus infection in vitro and in vivo[J].Journal of Fish Diseases,2019.DOI:10.1111/jfd.12987.[Epub ahead of print]
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