IGF2R基因多态性与胃癌易感性的相关性
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摘要
目的探讨IGF2R基因多态性与胃癌易感性的关系。方法提取华北地区105例胃癌患者及235例健康查体者外周血基因组DNA,用聚合酶链反应-限制性片段长度多态性(polymerase chain reation-restriction fragment length polymorphism,PCR-RFLP)方法检测IGF2R codon 901和codon 2020位点的基因型分布及差异。结果病例组codon 2020基因型AG、GG的分布频率显著低于对照组,且携基因型AG、GG者胃癌发病风险分别是携AA者的0.65倍(95%CI:0.38~0.89,P<0.05)和0.72倍(95%CI:0.45~0.91,P<0.05)。将AG、GG基因型合并计算,则其胃癌发病风险是AA基因型的0.68倍(95%CI:0.45~0.91,P<0.05)。Codon 901多态性在两组间分布差异无统计学意义(P>0.05)。结论 IGF2R基因codon 2020位点多态性可能对中国人群罹患胃癌具有保护作用。
Objective:To evaluate the correlations of polymorphisms of IGF2R gene with individal susceptibility to gastric cancer.Methods:Genomic DNA were extracted from peripheral blood leukocytes of 105 gastric cancer patients and 235healthy controls.Genotypes at codon 901 and codon 2020 polymorphic sites were identified by polymerase chain reactionrestriction fragment length polymorphism( PCR-RFLP).Results:At codon 2020,the frequency of AG or GG genotype was higher in the gastric cancer patients than in the healthy controls.As compared with AA genotype,AG,GG had significantly decreased risks of gastric cancer,with the decrease being 0.65-fold(( 95% CI:0.38 ~ 0.89,P < 0.05),0.72-fold( 95%CI:0.45 ~ 0.91,P < 0.05),respectively.The AG,GG genotypes combined,their risk of gastric cancer is 0.68-fold compared with AA genotype( 95% CI:0.45 ~ 0.91,P < 0.05).There was no significant difference of codon 901 polymorphism between the patients and the controls( P > 0.05).Conclusion:The polymorphism of IGF2R at codon 2020 might be potentially one of the protective factors against gastric cancer in Chinese population.
Objective:To evaluate the correlations of polymorphisms of IGF2R gene with individal susceptibility to gastric cancer.Methods:Genomic DNA were extracted from peripheral blood leukocytes of 105 gastric cancer patients and 235healthy controls.Genotypes at codon 901 and codon 2020 polymorphic sites were identified by polymerase chain reactionrestriction fragment length polymorphism( PCR-RFLP).Results:At codon 2020,the frequency of AG or GG genotype was higher in the gastric cancer patients than in the healthy controls.As compared with AA genotype,AG,GG had significantly decreased risks of gastric cancer,with the decrease being 0.65-fold(( 95% CI:0.38 ~ 0.89,P < 0.05),0.72-fold( 95%CI:0.45 ~ 0.91,P < 0.05),respectively.The AG,GG genotypes combined,their risk of gastric cancer is 0.68-fold compared with AA genotype( 95% CI:0.45 ~ 0.91,P < 0.05).There was no significant difference of codon 901 polymorphism between the patients and the controls( P > 0.05).Conclusion:The polymorphism of IGF2R at codon 2020 might be potentially one of the protective factors against gastric cancer in Chinese population.
引文
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[4]Feng-Ming Kong,Mitchell S Anscher,Mary Washington,et al.M6P/IGF2R is mutated in squamous cell carcinoma of the lung[J].Oncogene,2000,19(12):1572-1578.
[5]朱忠政,丛文铭,Hou Li-fang,等.IGF2R基因多态性与结直肠癌和肝癌遗传易感性的相关性研究[J].2008,29(11):1289-1292.