Box-Behnken响应面法优化健脾祛湿丸水提工艺
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摘要
目的采用Box-Behnken响应面法,优化健脾祛湿丸的水提工艺。方法以丹酚酸B、多糖含量及浸膏得率的综合评分为指标,在单因素试验基础上,采用Box-Behnken响应面法考察浸泡时间、加水倍量和提取时间对水提工艺的影响。结果最佳提取工艺为:浸泡1 h,加11倍量水,回流提取3次,每次1.20 h。结论优选的工艺方便、稳定、可行,可为后续研究提供依据。
Objective To optimize the water extraction process of Jianpi Qushi Concentrated Pills by using box-behnken response surface method. Methods Salvianolic acid B, total polysaccharide content and extract yield were set as the evaluation indexes. On the basis of single factor experiment results, the Box-Behnken response surface method was used to investigate the effects of soak time, adding water amount times and extracting time on the water extraction process. Results The optimum extraction technology was soaking for 1 h, with 11 times the amount of water extracted for three times, each time 1.20 h. Conclusion The optimum process is convenient, stable and feasible,which can provide references for follow-up study.
Objective To optimize the water extraction process of Jianpi Qushi Concentrated Pills by using box-behnken response surface method. Methods Salvianolic acid B, total polysaccharide content and extract yield were set as the evaluation indexes. On the basis of single factor experiment results, the Box-Behnken response surface method was used to investigate the effects of soak time, adding water amount times and extracting time on the water extraction process. Results The optimum extraction technology was soaking for 1 h, with 11 times the amount of water extracted for three times, each time 1.20 h. Conclusion The optimum process is convenient, stable and feasible,which can provide references for follow-up study.
引文
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[18]丛媛媛,热米拉·米吉提,帕丽达·阿不力孜,等.胀果甘草多糖对RAW264.7巨噬细胞免疫功能的影响[J].中华中医药学刊,2018,36(5):1043-1047.
[2]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:899-900,76-77.
[3]GONG X C, HUANG S C, PAE J Y, et al. Modeling of degradation kinetics of Salvianolic acid B at different temperatures and pH values[J]. Chinese Journal of Chemical Engineering,2017,25(1):68-73.
[4]耿琳,王婕,李欣,等.丹参制剂治疗银屑病的系统评价[J].中国中西医结合皮肤性病学杂志,2013,12(1):1-4.
[5]王娟.中药丹参的药用来源及研究[J].实用妇科内分泌杂志,2017,4(34):27-29.
[6]ZHENG P,FAN W,WANG S,et al.Characterization of polysaccharides extracted from Platycodon granddiflorus(Jacq.) A.DC. affecting activation of chicken peritoneal macrophages[J].International Journal of Biological Macromolecules,2017,96:775-785.
[7]LIU X, XIE J, JIA S, et al. Immunomodulatory effects of an acetylated Cyclocarya paliurus polysaccharide on murine macrophages RAW264.7[J].International Journal of Biological Macromolecules,2017,98:576.
[8]杨家强,车万莉,彭红艳,等.Box-Behnken响应面法优化荜茇总生物碱的提取工艺研究[J].中国药房,2018,29(13):1802-1805.
[9]冯彬彬,张建海,牛小花,等.Box-Behnken设计优化庙宇党参中党参炔苷和党参总多糖提取工艺[J].中国实验方剂学杂志,2015,21(24):18-21.
[10]孟玲,陈良,张刚,等.星点设计-效应面法优化玄芪化瘀浓缩丸水提工艺[J].中成药,2017,39(1):204-208.
[11]张江,张丽,木卡代斯·斯依提,等.响应面法优选银槐解毒颗粒提取工艺[J].中国中医药信息杂志,2018,25(1):64-68.
[12]李东,宗梁,郝福,等.Box-Behnken响应面法优选七味双花柴胡胶囊的水提工艺[J].中国现代中药,2018,20(1):88-92.
[13]陈亚楠,李美锋,董艳敏,等.中药多糖免疫调节作用研究进展[J].国际中医中药杂志,2016,38(9):855-858.
[14]苗明三.薏苡仁多糖对环磷酸酰胺致免疫抑制小鼠免疫功能的影响[J].中国医药学报,2002,30(5):49-50.
[15]BAE M J, SEE H J, CHOI G, et al. Regulatory T cell induced by Poria cocos bark exert therapeutic effects in murine models of atopic dermatitis and food allergy[J]. Mediators Inflamm,2016,2016:3472608.
[16]CHOI Y H. Induction of apoptosis by an ethanol extract of Poria cocos Wolf. in human leukemia U937 cells[J]. Oncol Rep,2015,34(5):2533-2540.
[17]张湘东,许定舟,李金华,等.丹参多糖的免疫调节活性研究[J].中药材,2012,35(6):949-952.
[18]丛媛媛,热米拉·米吉提,帕丽达·阿不力孜,等.胀果甘草多糖对RAW264.7巨噬细胞免疫功能的影响[J].中华中医药学刊,2018,36(5):1043-1047.