葛根芩连汤对2型糖尿病大鼠胰岛素抵抗及炎性因子的影响
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摘要
目的通过观察葛根芩连汤对2型糖尿病(T2DM)大鼠胰岛素抵抗及炎性因子的影响,探讨其改善胰岛素抵抗的作用机制。方法从40只大鼠中随机选取10只为空白组,余采用高脂高糖饮食饲养方法加注射小剂量链脲佐菌素(STZ)制备T2DM模型。将模型制备成功的大鼠随机分为模型组、罗格列酮组和葛根芩连汤组。按10 mL/kg给予空白组和模型组大鼠0.9%氯化钠溶液,同时罗格列酮组和葛根芩连汤组分别被给予罗格列酮溶液3 mg/kg和葛根芩连汤18.2 g/kg灌胃,1次/d,共4周。运用血糖仪检测各组大鼠FBG,以及放射免疫法检测FINS水平,并计算HOMA-IR;采用ELISA检测各组大鼠血清炎性因子TNF-α、IL-6、IL-1β以及MCP-1的水平。结果与模型组比较,罗格列酮组大鼠的FBG显著下降(P <0.01);与模型组比较,罗格列酮组和葛根芩连汤组大鼠的FINS、HOMA-IR及血清TNF-α、IL-6、IL-1β、MCP-1水平明显降低(P <0.01或P <0.05)。结论葛根芩连汤可改善T2DM大鼠胰岛素抵抗,该作用可能与抑制炎性因子释放有关。
Objective To observe the effect of Gegen Qinlian Decoction on insulin resistance and inflammatory factors in type 2 diabetes mellitus(T2 DM) rats and explore the mechanism of ef fect. Methods Ten rats were randomly selected from 40 T2 DM rats as blank group, and other 30 rats were fed with high fat and sugar diet and injected lowdose streptozotocin(STZ) to prepare the model of T2 DM. The successful T2 DM model rats were randomly divided into model group, rosiglitazone group and Gegen Qinlian Decoction group. The rats of blank group and model group were given 0.9% sodium chloride solution(10 mL/kg), moreover the rats of rosiglitazone group and Gegen Qinlian Decoction group were given rosiglitazone solution 3 mg/kg and Gegen Qinlian Decoction 18.2 g/kg respectively, once a day for 4 weeks. FBG was measured by blood glucose meter, and FINS was measured by radioimmunoassay and then HOMA-IR was calculated. The levels of serum inflammatory factors, including TNF-α, IL-6, IL-1β and MCP1,were detected by ELISA. Results Compared with the model group, FBG in rosiglitazone group was significantly decreased(P < 0.01), but there were no significant difference between Gegen Qinlian Decoction group and model group rosiglitazone group. Compared with model group, the levels of FINS, HOMA-IR and serum TNF-α, IL-6,IL1β and MCP-1 in rosiglitazone group and Gegen Qinlian Decoction group were significantly decreased(P < 0.01 or P < 0.05). Conclusion Gegen Qinlian Decoction could improve insulin resistance in T2 DM rats, which might be related to inhibit the release of inflammatory factors.
Objective To observe the effect of Gegen Qinlian Decoction on insulin resistance and inflammatory factors in type 2 diabetes mellitus(T2 DM) rats and explore the mechanism of ef fect. Methods Ten rats were randomly selected from 40 T2 DM rats as blank group, and other 30 rats were fed with high fat and sugar diet and injected lowdose streptozotocin(STZ) to prepare the model of T2 DM. The successful T2 DM model rats were randomly divided into model group, rosiglitazone group and Gegen Qinlian Decoction group. The rats of blank group and model group were given 0.9% sodium chloride solution(10 mL/kg), moreover the rats of rosiglitazone group and Gegen Qinlian Decoction group were given rosiglitazone solution 3 mg/kg and Gegen Qinlian Decoction 18.2 g/kg respectively, once a day for 4 weeks. FBG was measured by blood glucose meter, and FINS was measured by radioimmunoassay and then HOMA-IR was calculated. The levels of serum inflammatory factors, including TNF-α, IL-6, IL-1β and MCP1,were detected by ELISA. Results Compared with the model group, FBG in rosiglitazone group was significantly decreased(P < 0.01), but there were no significant difference between Gegen Qinlian Decoction group and model group rosiglitazone group. Compared with model group, the levels of FINS, HOMA-IR and serum TNF-α, IL-6,IL1β and MCP-1 in rosiglitazone group and Gegen Qinlian Decoction group were significantly decreased(P < 0.01 or P < 0.05). Conclusion Gegen Qinlian Decoction could improve insulin resistance in T2 DM rats, which might be related to inhibit the release of inflammatory factors.
引文
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[11]王萌,张会存,刘欣,等.参苓白术散对非酒精性脂肪性肝病模型大鼠瘦素及胰岛素抵抗的影响[J].中国中医药信息杂志,2018,25(10):35-39.
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[14]LONTCHIYIMAGOU E,SOBNGWI E,MATSHA T E,et al.Diabetes mellitus and inflammation[J].Current Diabetes Reports,2013,13(3):435-444.
[15]李永荣,程涛,王永胜,等.富硒黄芪提取物对链脲霉素诱导的糖尿病大鼠胰岛素抵抗的影响[J].中国药科大学学报,2018,49(6):739-745.
[16]徐麟皓,张晓明,王征.雷帕霉素抑制糖尿病大鼠肾Akt/NF-κB信号通路介导的炎症反应的机制研究[J].中国细胞生物学学报,2015,37(9):1263-1270.
[17]王烨,周琦,朱向东,等.葛根芩连汤对自发肥胖型2型糖尿病ZDF大鼠FFA及NF-κB/IRS2通路的影响[J].上海中医药大学学报,2017,31(6):65-69.
[18]赵金龙,汤顺莉,陈国铭,等.基于系统药理学的葛根芩连汤治疗2型糖尿病作用机制探讨[J].中国实验方剂学杂志,2018,24(12):199-209.
[19]王新,张巍巍,杨婧,等.葛根芩连汤对感染大肠杆菌小鼠血常规、血液内毒素及炎性细胞因子含量的影响[J].中国兽药杂志,2019,53(4):52-58.
[20]高东,邵喜风.葛根芩连汤治疗溃疡性结肠炎的疗效及对患者炎性反应和免疫功能的影响[J].解放军医药杂志,2018,30(10):89-92.
[2]吴青华,李冰涛,涂珺.复方中药治疗糖尿病的研究进展[J].中国中药杂志,2019,44(6):1104-1109.
[3]范尧夫,曹雯,胡咏新,等.葛根芩连汤对新发2型糖尿病胰岛素抵抗的影响研究[J].现代中西医结合杂志,2017,26(2):115-117,121.
[4]王雷,成华.基于代谢组学方法探索葛根芩连汤方剂治疗2型糖尿病大鼠的效果研究[J].环球中医药,2019,12(1)19-23.
[5]张昌林,吴荣焕,盛泓沁,等.基于网络药理学的葛根芩连汤治疗2型糖尿病的效应机制[J].中国实验方剂学杂志,2018,24(19):179-188.
[6]王烨,朱向东.葛根芩连汤对2型糖尿病ZDF大鼠CRP,TNF-α,IL-6的影响[J].中国实验方剂学杂志,2017,23(21):130-134.
[7]孙晓芳,赵长勇,陈晖,等.高糖高脂饮食加链尿佐菌素建立实验性大鼠2型糖尿病模型[J].南京医科大学学报(自然科学版),2009,29(6):797-800,806.
[8]OZBARER C,KURT H,KEBAPCI M N,et al.Effects of geneticvariations in the genes encoding NOD1 and NOD2 on type 2 diabetes mellitus and insulin resistance[J].Journal of clinical Pharmacy and Therapeutics,2017,42(1):98-102.
[9]ROSE D P,GRACHECK P J,VONADAVIS L.The interactions of obesity,inflammation and insulin resistance in breast cancer[J].Cancers,2015,7(4):2147-2168.
[10]KHODABANDEHOLL H,GORGANIFIRUZJAEE S,PANAHIS G,et al.Molecular and cellular mechanisms linking inflammation to insulin resistance andβ-cell dysfunction[J].Translational Reseatch,2015,167(1):228-256.
[11]王萌,张会存,刘欣,等.参苓白术散对非酒精性脂肪性肝病模型大鼠瘦素及胰岛素抵抗的影响[J].中国中医药信息杂志,2018,25(10):35-39.
[12]AKASH M S,REHMAN K,CHEN S.Role of inflammatory mechanismsin pathogenesis of type 2 diabetes mellitus[J].Journal of Cellular Biochemistry,2013,114(3):525-531.
[13]KANDA H,TATEYA S,TAMORI Y,et al.MCP-1contributes to macrophage infiltration into adipose tissue,insulin resistance,and hepatic steatosis in obesity[J].Journal of Central South University,2010,17(3):472-479.
[14]LONTCHIYIMAGOU E,SOBNGWI E,MATSHA T E,et al.Diabetes mellitus and inflammation[J].Current Diabetes Reports,2013,13(3):435-444.
[15]李永荣,程涛,王永胜,等.富硒黄芪提取物对链脲霉素诱导的糖尿病大鼠胰岛素抵抗的影响[J].中国药科大学学报,2018,49(6):739-745.
[16]徐麟皓,张晓明,王征.雷帕霉素抑制糖尿病大鼠肾Akt/NF-κB信号通路介导的炎症反应的机制研究[J].中国细胞生物学学报,2015,37(9):1263-1270.
[17]王烨,周琦,朱向东,等.葛根芩连汤对自发肥胖型2型糖尿病ZDF大鼠FFA及NF-κB/IRS2通路的影响[J].上海中医药大学学报,2017,31(6):65-69.
[18]赵金龙,汤顺莉,陈国铭,等.基于系统药理学的葛根芩连汤治疗2型糖尿病作用机制探讨[J].中国实验方剂学杂志,2018,24(12):199-209.
[19]王新,张巍巍,杨婧,等.葛根芩连汤对感染大肠杆菌小鼠血常规、血液内毒素及炎性细胞因子含量的影响[J].中国兽药杂志,2019,53(4):52-58.
[20]高东,邵喜风.葛根芩连汤治疗溃疡性结肠炎的疗效及对患者炎性反应和免疫功能的影响[J].解放军医药杂志,2018,30(10):89-92.