烤烟EMS诱变后代高香气突变体筛选

详细信息    查看全文 | 推荐本文 |

英文篇名：Screening of High Aroma Mutants from Progenies of EMS Mutagenized Flue-Cured Tobacco 作者：杨欣玲 ; 姚倩 ; 平文丽 ; 马一琼 ; 王宝林 ; 贾国涛 ; 杨永锋 ; 崔红 英文作者：Yang Xinling;Yao Qian;Ping Wenli;Ma Yiqiong;Wang Baolin;Jia Guotao;Yang Yongfeng;Cui Hong;Technology Centre, China Tobacco Henan Industrial Co., Ltd.;Institute ofTobacco Research, HAAS/Key Laboratory for Green Preservation & Control of Tobacco Disease & Pests inHuanghuai Tobacco Growing Area;College of Tobacco Science,Henan Agricultural University; 关键词：烤烟 ; EMS诱变技术 ; 腺毛密度 ; 化学成分 ; 香气物质 ; 突变体筛选 英文关键词：Flue-cured tobacco;;EMS induced mutation;;Glandular trichomes density;;Chemical components;;Aromasubstance;;Mutant screening 中文刊名：ZWZZ 英文刊名：Crops 机构：河南中烟工业有限责任公司技术中心;河南省农业科学院烟草研究所/黄淮烟区烟草病虫害绿色防控重点实验室;河南农业大学烟草学院; 出版日期：2019-02-01 16:50 出版单位：作物杂志 年：2019 期：No.188 基金：河南中烟工业有限责任公司项目(ZW2014004、ZW2014005) 语种：中文; 页：ZWZZ201901012 页数：7 CN：01 ISSN：11-1808/S 分类号：74-80

摘要

为筛选高香气且品质较优的烤烟后代,以中烟100为材料,采用EMS诱变技术对中烟100种子进行处理,筛选出2个叶面腺毛密度大的突变体材料,通过田间试验,对其叶面腺毛密度、叶面化学成分、烤后化学成分、香气物质含量和感官质量评吸等指标进行评估,并与正常中烟100后代和NC89进行对照。研究结果表明,EMS-2诱变株系腺毛密度最大,为65.55个/mm~2,分别是中烟100(37.19个/mm~2)和NC89(56.06个/mm~2)的1.8和1.2倍;叶面化学成分分析结果表明,EMS-1诱变株系西柏烷类和烷烃类含量最多,分别为57.18、5.16mg/m~2,EMS-2突变株系蔗糖酯类物质分泌较旺盛,含量最多,为0.33mg/m~2;烤后样化学成分分析结果表明,EMS-2突变株系化学成分含量较协调,且香气成分含量最多,达到890.32μg/g,分别比中烟100和NC89增加34.5%和29.0%;结合对诱变后代的感官评价,得出结论:诱变株系综合品质较好,可提高烟叶质量,EMS-2综合品质最好,可以作为待选株系进行下一步品种选育。
        In order to screen the high-aroma and high-quality flue-cured tobacco offspring, Zhongyan 100 was usedas the experimental material, and EMS mutagenesis technology was employed to treat the Zhongyan 100 seed andscreen out two mutations with high density of leaf glandular trichomes. The body material was evaluated by field testfor its glandular trichomes density, chemical composition of the leaf surface, chemical composition after baking, aromasubstance content and sensory quality evaluation, and compared with the normal tobacco Zhongyan 100 offspring andNC89. The results showed that the density of glandular trichomes of EMS-2 mutagenized strains was 1.8 times and 1.2 times that of Zhongyan 100 and NC89, respectively. The chemical composition analysis of leaf surface showed thatEMS-1 mutagenized strains the contents of cembranes and alkanes were the highest. The content of the sucrose estersin the EMS-2 mutant strain was the most abundant. The chemical composition analysis of the EMS-2 mutant strainshowed that the chemical components of the EMS-2 mutant strain were more harmonious, and the aroma componentswere the highest. Compared with Zhongyan 100 and NC89, it increased by 34.5% and 29.0%. Combined with thesensory evaluation of mutagenized offspring, it was concluded that the comprehensive quality of mutagenic strains wasbetter, and the quality of tobacco leaves could be improved. The comprehensive quality of EMS-2 was the best andcould be used as selected strains proceed to the next breeding.

引文

[1]孙计平,吴照辉,李雪君,等.烤烟品种中烟100与NC89及其F1主要特性研究.河南农业科学,2014,43(9):46-51,55.
    [2]张杨,裴军,王方峰,等.不同施肥水平对中烟100产质量的影响.中国烟草科学,2007(6):33-35.
    [3]王正旭,向德恩,孟贵星,等.施氮量和留叶数对中烟100产质量的影响.中国烟草科学,2011(S1):45-49.
    [4]李章海,潘文杰,朱晓兰,等.不同烘烤方式烘烤过程中烟叶表面腺毛分泌物变化的研究.中国烟草学报,2011(6):81-85.
    [5]王伟,孔光辉,李佛琳,等.烤烟烟叶腺毛及其分泌物研究进展.中国农学通报,2007(2):251-254.
    [6]平文丽,金立锋,张小全,等.快中子辐射诱变K326突变体库的创建及初步分析.烟草科技,2018,51(1):21-27.
    [7]朱晓宇,姚珊珊,赵宸楠,等. DIS过程中烤烟叶片腺毛形态结构及分泌物变化.江西农业学报,2010(5):9-11.
    [8]崔清志,刘晓虹,陈惠明. EMS诱变技术研究进展.湖南农业科学,2013(5):7-9.
    [9]杨攀,雷振生,吴政卿,等.航天诱变小麦新品种郑麦3596的选育.作物杂志,2014(5):163-167.
    [10]李小六,张运峰,张淑红,等.原生质体EMS诱变选育杏鲍菇高产菌丝多糖菌株.核农学报,2017(8):1463-1468.
    [11]佟星,赵波,金文林,等.小豆EMS诱变M3代叶形突变体的筛选及农艺性状分析.安徽农业科学,2010(9):4472-4474.
    [12]侯睿,岳福良,张小红,等. EMS诱变对花生油酸、蛋白质含量的影响.西南农业学报,2016(6):1245-1249.
    [13]姜颖,孙宇峰,潘冬梅,等.工业大麻甲基磺酸乙酯(EMS)诱变体的筛选及RAPD分析.作物杂志,2017(6):50-54.
    [14]高志勇,谢恒星,王志平,等.植物突变体库的作用及构建研究进展.作物杂志,2016(6):16-19.
    [15]刘威,杨峰,任旭东,等. EMS对种子萌发影响的进展.分子植物育种,2017,15(11):4585-4589.
    [16]韩锦峰,张志勇,刘华山,等.烟草腺毛及其分泌物西柏三烯醇类物质的研究进展.中国烟草学报,2013(5):118-124.
    [17]王瑞新.烟草化学.北京:中国农业出版社,2003:1305-1312.
    [18]李艳华,张洪映,魏跃伟,等.典型烤烟品种腺毛形态及分泌特性比较分析.中国烟草学报,2017,23(2):84-91.
    [19]杜咏梅,郭承芳,张怀宝,等.水溶性糖、烟碱、总氮含量与烤烟吃味品质的关系研究.中国烟草科学,2000(1):9-12.
    [20]何承刚,曾旭波.烤烟香气物质的影响因素及其代谢研究进展.中国烟草科学,2005(2):40-43.
    [21]陈顺辉,李文卿,江荣风,等.施氮量对烤烟产量和品质的影响.中国烟草学报,2003(S):36-40.
    [22]刘贯山.烟草突变体的筛选与鉴定.中国烟草科学,2012(1):102-103.
    [23]叶敬忠,李华.关于转基因技术的综述与思考.农业技术经济,2014(1):11-21.
    [24]过伟民,张艳玲,蔡宪杰,等.光质对烤烟品质及光合色素含量的影响.烟草科技,2011(9):65-70.
    [25]史宏志,官春云.烟草腺毛分泌物的化学成分及遗传.作物研究,1995,9(3):46-49.
    [26]付玉兰,杨焕文,邓建华,等.津巴布韦引进与云南主栽烤烟品种腺毛密度及分泌物的差异.烟草科技,2012(9):82-86.
    [27]薛晓明.烤烟腺毛的形态学观察和密度研究.中国农学通报,2012(4):98-103.
    [28]杨铁钊,李伟,李钦奎,等.烤烟叶面腺毛密度及其分泌物变化动态的相关分析.中国烟草科学,2005(1):43-46.
    [29]王宝华,吴帼英,黄静勲.烟叶植物学特性的观察Ⅰ、烤烟烟叶腺毛的密度及其与烟叶品质和化学成分的关系.中国烟草科学,1983(2):1-6.
    [30]周昆,周清明,胡晓兰.烤烟香气物质研究进展.中国烟草科学,2008,29(2):58-61.
    [31]薛晓明,张晶,于丽杰.烤烟腺毛分泌细胞的超微结构观察.贵州农业科学,2011(8):45-48.
    [32]龚丝雨,钟思荣,张世川,等.增施生物炭对烤烟生长及产量、质量的影响.作物杂志,2018(2):154-160.
    [33]陆永恒.生态条件对烟叶品质影响的研究进展.中国烟草科学.2007(3):43-46.
    [34]高华军,林北森.生物钾肥对烤烟产质量影响的研究进展.中国烟草科学,2009(3):73-76.
    [35]刘燕,孙焕良,李辉,等.烟草腺毛密度和分泌物与烟叶品质的关系.作物研究,2012,26(6):737-739.