CYP11A1基因rsrs900798位点多态性与老年性骨质疏松性骨折骨密度、骨代谢标志物的相关性研究
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摘要
目的探讨CYP11A1基因rsrs900798位点多态性与老年性骨质疏松性骨折患者骨密度、骨代谢标志物的关联。方法前瞻性选取2016年4月至2018年4月骨科收治的骨质疏松症及骨质疏松性骨折患者共420例,其中220例老年骨质疏松症患者作为对照组,200例老年骨质疏松性骨折患者作为观察组。采用SNa Pshot技术单核苷酸多态性(SNP)分型,测定患者骨密度、骨代谢标志物[血清总骨Ⅰ型前胶原氨基端前肽(Total-PINP)、Ⅰ型胶原羧基端肽β特殊序列(β-CTX)]。观察该基因多态性与患者骨密度、骨代谢标志物的关联。结果观察组与对照组等位基因频率、基因型频率差异均无显著性(P>0.05)。观察组Total-PINP、β-CTX均高于对照组(P<0.05)。观察组GG、GT、TT三种基因型的Total-PINP、β-CTX检测含量差异均无统计学意义(P>0.05)。总髋部、腰椎、股骨大转子、股骨颈、Ward's三角5个部位GG、GT、TT三种基因型骨密度差异均无统计学意义(P>0.05)。结论骨代谢标志物PINP和β-CTX可用来预测骨折危险性,但与CYPll Al基因rs900798位点多态性和其无明显关联,且该位点与骨折患者骨密度无明显关联。
Objective To explore the correlation of Rsrs900798 CYP11A1 gene loci polymorphism and senile osteoporosis fracture in patients with bone mineral density and bone metabolism markers. Methods From April 2016 to April 2018,a total of 420 osteoporosis and osteoporotic fractures patients admitted in our hospital were enrolled in this study,of which 220 cases of senile osteoporosis patients were selected as con-trol group,200 cases of elderly patients with osteoporotic fracture as observation group. SNa Pshot technology single nucleotide polymorphisms( SNPS) classification was used to determine bone mineral density and bone metabolism in patients with markers( Total-PINP、β-CTX). The correlation of gene polymorphisms and bone mineral density,bone metabolic markers was analyzed. Results The allele frequency and genotype frequency in the two groups had no significant difference( P > 0. 05). Total-PINP,beta CTX in observation group were higher than those of control group( P < 0. 05). Total-PINP,beta CTX of GG,GT,TT three genotypes in observation group had no statistically significant difference( P > 0. 05). GG,GT,TT of total hip,lumbar spine,femoral greater trochanter,femoral neck,Ward's triangle had no statistically significant difference in the bone mineral density( P > 0. 05). Conclusion Bone metabolic markers PINP and beta CTX can be used to predict fracture risk,but its correlation with rs900798 gene loci polymorphism of CYPll Al is not obvious,and the loci is not obviously correlated with bone mineral density of fracture patients.
Objective To explore the correlation of Rsrs900798 CYP11A1 gene loci polymorphism and senile osteoporosis fracture in patients with bone mineral density and bone metabolism markers. Methods From April 2016 to April 2018,a total of 420 osteoporosis and osteoporotic fractures patients admitted in our hospital were enrolled in this study,of which 220 cases of senile osteoporosis patients were selected as con-trol group,200 cases of elderly patients with osteoporotic fracture as observation group. SNa Pshot technology single nucleotide polymorphisms( SNPS) classification was used to determine bone mineral density and bone metabolism in patients with markers( Total-PINP、β-CTX). The correlation of gene polymorphisms and bone mineral density,bone metabolic markers was analyzed. Results The allele frequency and genotype frequency in the two groups had no significant difference( P > 0. 05). Total-PINP,beta CTX in observation group were higher than those of control group( P < 0. 05). Total-PINP,beta CTX of GG,GT,TT three genotypes in observation group had no statistically significant difference( P > 0. 05). GG,GT,TT of total hip,lumbar spine,femoral greater trochanter,femoral neck,Ward's triangle had no statistically significant difference in the bone mineral density( P > 0. 05). Conclusion Bone metabolic markers PINP and beta CTX can be used to predict fracture risk,but its correlation with rs900798 gene loci polymorphism of CYPll Al is not obvious,and the loci is not obviously correlated with bone mineral density of fracture patients.
引文
[1]陈晓虹,沈慧,夏杰琼.中老年人骨质疏松性骨折流行病学特点及相关因素[J].中国老年学杂志,2016,36(13):3299-3300,3301.
[2]高贵花.CYP11A1基因多态性与多囊卵巢综合征关联研究[D].合肥:安徽医科大学,2009.
[3]中华医学会骨质疏松和骨矿盐疾病分会.原发性骨质疏松症诊治指南(2011年)[J].中华骨质疏松和骨矿盐疾病杂志,2011,4(1):2-17.
[4]张巧慧,柳洁.同型半胱氨酸水平及MTHFR C677T基因多态性与中老年男性骨密度相关性分析[J].中国药物与临床,2018,18(2):195-198.
[5]方志峰,陈玉柱.骨质疏松相关VDR、ER基因多态性研究进展[J].应用预防医学,2014,16(4):254-256.
[6]张奎,刘洋,马煜,等.骨质疏松性骨折与相关基因多态性研究进展[J].中国骨质疏松杂志,2017,23(7):974-980.
[7]李昕,田京.雌激素受体α基因多态性与骨质疏松关系的研究进展[J].中国骨质疏松杂志,2013,19(3):297-301.
[8]马秋华,周晓辉.骨质疏松相关基因研究进展[J].中国老年学杂志,2014,(20):5929-5931.
[9]中华医学会骨科学分会骨质疏松学组.骨质疏松性骨折诊疗指南[J].中华骨科杂志,2017,37(1):1-10.
[10]骨质疏松流行病学现状[J].中华医学杂志,2009,89(42):2953-2955.
[11]游利.骨质疏松症的现状、筛查和预防[J].中国全科医学,2016,19(14):1616-1619.
[12]Harvey N,Dennison E,Cooper C.Osteoporosis:impact on health and economics[J].Nat Rev Rheumatol,2010,6(2):99-105.
[13]Rodríguez-Sanz M,García-Giralt N,Prieto-Alhambra D,et al.CYP11A1 expression in bone is associated with aromatase inhibitor-related bone loss[J].J Mol Endocrinol,2015,55(1):69-79.
[14]孔德策,杨铁毅,邵进.绝经后骨质疏松骨代谢标志物研究进展[J].国际骨科学杂志,2016,37(1):36-41.
[15]唐颂军,宋力轶,朱文峰,等.骨转换标志物PINP和β-CTX的测定在预测骨质疏松性骨折中的价值[J].实用临床医药杂志,2015,19(21):17-19.
[16]公爱凤.骨代谢标志物25(OH)D3、β-CTX和Total-PⅠNP在老年骨质疏松症患者髋部脆性骨折诊断中的检测价值[J].临床和实验医学杂志,2017,16(6):555-558.
[17]王其飞,刘正,刘家帮,等.CYP11A1基因多态性与老年性骨质疏松性骨折相关性研究[J].中国骨质疏松杂志,2017,23(3):303-306,367.
[2]高贵花.CYP11A1基因多态性与多囊卵巢综合征关联研究[D].合肥:安徽医科大学,2009.
[3]中华医学会骨质疏松和骨矿盐疾病分会.原发性骨质疏松症诊治指南(2011年)[J].中华骨质疏松和骨矿盐疾病杂志,2011,4(1):2-17.
[4]张巧慧,柳洁.同型半胱氨酸水平及MTHFR C677T基因多态性与中老年男性骨密度相关性分析[J].中国药物与临床,2018,18(2):195-198.
[5]方志峰,陈玉柱.骨质疏松相关VDR、ER基因多态性研究进展[J].应用预防医学,2014,16(4):254-256.
[6]张奎,刘洋,马煜,等.骨质疏松性骨折与相关基因多态性研究进展[J].中国骨质疏松杂志,2017,23(7):974-980.
[7]李昕,田京.雌激素受体α基因多态性与骨质疏松关系的研究进展[J].中国骨质疏松杂志,2013,19(3):297-301.
[8]马秋华,周晓辉.骨质疏松相关基因研究进展[J].中国老年学杂志,2014,(20):5929-5931.
[9]中华医学会骨科学分会骨质疏松学组.骨质疏松性骨折诊疗指南[J].中华骨科杂志,2017,37(1):1-10.
[10]骨质疏松流行病学现状[J].中华医学杂志,2009,89(42):2953-2955.
[11]游利.骨质疏松症的现状、筛查和预防[J].中国全科医学,2016,19(14):1616-1619.
[12]Harvey N,Dennison E,Cooper C.Osteoporosis:impact on health and economics[J].Nat Rev Rheumatol,2010,6(2):99-105.
[13]Rodríguez-Sanz M,García-Giralt N,Prieto-Alhambra D,et al.CYP11A1 expression in bone is associated with aromatase inhibitor-related bone loss[J].J Mol Endocrinol,2015,55(1):69-79.
[14]孔德策,杨铁毅,邵进.绝经后骨质疏松骨代谢标志物研究进展[J].国际骨科学杂志,2016,37(1):36-41.
[15]唐颂军,宋力轶,朱文峰,等.骨转换标志物PINP和β-CTX的测定在预测骨质疏松性骨折中的价值[J].实用临床医药杂志,2015,19(21):17-19.
[16]公爱凤.骨代谢标志物25(OH)D3、β-CTX和Total-PⅠNP在老年骨质疏松症患者髋部脆性骨折诊断中的检测价值[J].临床和实验医学杂志,2017,16(6):555-558.
[17]王其飞,刘正,刘家帮,等.CYP11A1基因多态性与老年性骨质疏松性骨折相关性研究[J].中国骨质疏松杂志,2017,23(3):303-306,367.
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