大花紫薇及小叶紫薇花气味化学成分分析
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摘要
【目的】明确大花紫薇及小叶紫薇的花香气化学成分。【方法】应用动态顶空吸附法收集大花紫薇(Lagerstroemia speciosa)及小叶紫薇(Lagerstroemia indica)花气味,结合应用气相色谱-质谱(GC-MS)技术分离鉴定二者化学成分,采用离子流峰面积归一化法计算花气味中各挥发物组分的成分。【结果】大花紫薇及小叶紫薇花香分析分别鉴定出28种及39种物质。比较发现16种相同化合物,二者花气味共分析鉴定出47种化合物,其中烷类化合物(32%)、苯环类芳香族化合物(26%)及单萜和倍半萜类化合物(23%)为二者主要成分,占总成分的81%,二者含量最高的物质分别为10-甲基十九烷(64.9%),β-蒎烯(21.6%)。【结论】该文为进一步探究大花紫薇和小叶紫薇及它们与传粉昆虫之间互利共生关系的化学生态学方面的研究提供理论基础。
【Objective】The present paper aimed to find out the chemical composition of the floral scent of Lagerstroemia grandiflora and Lagerstroemia crassifolia. 【Method】The dynamic headspace adsorption technique was employed to collect the floral scent emitted by Lagerstroemia speciose and Lagerstroemia indica,using the gas chromatography coupled with mass spectrometry(GC-MS) for the detection and identification of volatile chemical components in Lagerstroemia speciose and Lagerstroemia indica, and the peak area normalization was used to determine the relative contents of each odour component. 【Result】28 species in Lagerstroemia speciose and 39 species in Lagerstroemia indica were found. After comparison, 16 kinds of identical compounds were found, both of the flowers were found total 47 compounds, of which, Alkane Compounds(32 %), benzene ring aromatic compounds(26 %), monoterpenes and sesquiterpenes(23 %) were the main components of two flowers, accounting for 81 % of the total components, and the highest contents of the two flowers were 10-methylnonadecane(64.9 %) and β-pinene(21.6 %). 【Conclusion】The study provided the basic data for further investigations of the symbiotic relationships between Lagerstroemia speciose and Lagerstroemia indica and its pollinating insects.
【Objective】The present paper aimed to find out the chemical composition of the floral scent of Lagerstroemia grandiflora and Lagerstroemia crassifolia. 【Method】The dynamic headspace adsorption technique was employed to collect the floral scent emitted by Lagerstroemia speciose and Lagerstroemia indica,using the gas chromatography coupled with mass spectrometry(GC-MS) for the detection and identification of volatile chemical components in Lagerstroemia speciose and Lagerstroemia indica, and the peak area normalization was used to determine the relative contents of each odour component. 【Result】28 species in Lagerstroemia speciose and 39 species in Lagerstroemia indica were found. After comparison, 16 kinds of identical compounds were found, both of the flowers were found total 47 compounds, of which, Alkane Compounds(32 %), benzene ring aromatic compounds(26 %), monoterpenes and sesquiterpenes(23 %) were the main components of two flowers, accounting for 81 % of the total components, and the highest contents of the two flowers were 10-methylnonadecane(64.9 %) and β-pinene(21.6 %). 【Conclusion】The study provided the basic data for further investigations of the symbiotic relationships between Lagerstroemia speciose and Lagerstroemia indica and its pollinating insects.
引文
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[12]陈旭鹏,杨美红,张金桐,等. 用于植物挥发物分析的PoraPakTM Q吸附管快速活化方法[J]. 亚热带植物学,2015,44(3):209-212.
[13]权春梅,周光姣,朱勇,等. 水蒸气蒸馏法提取芍花精油研究[J]. 长江大学学报(自科版),2017,14(8):8-11.
[14]付国需,李为争,刘坷,等. 不同科芳香植物特征挥发物的生源及其对昆虫行为的影响[J]. 华中昆虫研究,2008(5):14-27.
[15]罗长维. 麻疯树传粉昆虫组成及主要传粉者行为生态学研究[J]. 北京:中国林业科学研究院,2012.
[16]汪佳晴,穆丹,刘正奎,等. 18 种植物挥发物对柳黑毛蚜行为的调控效应[J]. 热带作物学报,2013,34(8):1539-1543.
[17]李耀发,高占林,康云凤,等. 寄主植物花器挥发性物质分析及其对绿盲蝽成虫的引诱作用[J]. 河北农业大学学报,2014,37(4):96-100.
[18]张雯雯. 蝴蝶蜜源及寄主植物挥发物成分的研究[D]. 北京:中国林业科学研究院,2011.
[19]穆丹,赵傲,刘娜,等. 植物挥发物对夹竹桃蚜的引诱效应[J]. 安徽农学通报,2015,21(24):37-38.
[20]蔡晓明,李兆群,潘洪生,等. 植食性害虫食诱剂的研究与应用[J]. 中国生物防治学报,2018,34(1):28-35.
[2]王伟. 小菊花部特征及花冠精油组分与访花昆虫的相关性[D]. 南京:南京农业大学,2008:1-15.
[3]BAO X X,LIAN Y, BAO XP. Comparatively research progress on extraction methods of plant flavoring compositions[J]. Inner Monggolia Agricultural Science and Technology, 2016, 44(5): 126-130.
[4]HUANG D H, ZHANG Z G, CHEN G P, et al. Analysis of the components of floral scent inn Glochidion puberum using gas chromatography-mass spectrometry with dynamic headspave adsorption[J]. Chinese Journal of Chromatography, 2015, 33(3): 318-322.
[5]王燕,詹勤,席忠新,等. 紫薇属植物的化学成分和药理作用研究进展[J]. 药学实践杂志, 2010,28(2):88-91.
[6]周理华,周金琳,潘水年. 紫薇生物学特性及其在庆元城区公园绿地中的应用[J]. 现代农业科技,2011(5):203-204.
[7]王燕,孙连娜,楼永明. 大花紫薇化学成分与药理作用研究进展[J]. 福建分析测试,2014,23(5):20-24.
[8]游振城. 小叶紫薇裸根大苗移栽试验[J]. 内蒙古林业调查设计,2012,35(1):33-34.
[9]ZHANG Z G, TENG K J, LI H H. Biological characteristics of Epicephala ancylopa (Lepidoptera: Gracillariidae) on host Glochidion sp.(Phyllanthaceae) and the compositional analysis of its floral scent[J].Acta Entomologica Sinica, 2016, 59(6): 669-681.
[10]Svensson G P, Okamoto T, Kawakita A, et al. Chemical ecology of obligate pollination mutualisms: testing the‘private channel'hypothesis in the Breynia-Epicephala association[J]. New Phytol, 2010, 186: 995-1004.
[11]孔杜林,陈衍成,范超军,等. 大花紫薇叶挥发油化学成分研究[J]. 海南师范大学学报(自然科学版),2013,26(1):37-39.
[12]陈旭鹏,杨美红,张金桐,等. 用于植物挥发物分析的PoraPakTM Q吸附管快速活化方法[J]. 亚热带植物学,2015,44(3):209-212.
[13]权春梅,周光姣,朱勇,等. 水蒸气蒸馏法提取芍花精油研究[J]. 长江大学学报(自科版),2017,14(8):8-11.
[14]付国需,李为争,刘坷,等. 不同科芳香植物特征挥发物的生源及其对昆虫行为的影响[J]. 华中昆虫研究,2008(5):14-27.
[15]罗长维. 麻疯树传粉昆虫组成及主要传粉者行为生态学研究[J]. 北京:中国林业科学研究院,2012.
[16]汪佳晴,穆丹,刘正奎,等. 18 种植物挥发物对柳黑毛蚜行为的调控效应[J]. 热带作物学报,2013,34(8):1539-1543.
[17]李耀发,高占林,康云凤,等. 寄主植物花器挥发性物质分析及其对绿盲蝽成虫的引诱作用[J]. 河北农业大学学报,2014,37(4):96-100.
[18]张雯雯. 蝴蝶蜜源及寄主植物挥发物成分的研究[D]. 北京:中国林业科学研究院,2011.
[19]穆丹,赵傲,刘娜,等. 植物挥发物对夹竹桃蚜的引诱效应[J]. 安徽农学通报,2015,21(24):37-38.
[20]蔡晓明,李兆群,潘洪生,等. 植食性害虫食诱剂的研究与应用[J]. 中国生物防治学报,2018,34(1):28-35.