高氧气调包装抑制菜心木质化关键酶苯丙氨酸酶的研究
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摘要
探究高氧气调包装对影响鲜切菜心木质化的苯丙氨酸酶(phenylalaninammo-nialyase,PAL)的影响。采用高氧(氧含量大于90%)气调包装对鲜切菜心进行处理,以空气包装鲜切菜心作对照,置于25℃下贮藏,然后检测其木质素含量、PAL活性,并通过反转录PCR(reverse transcription-PCR,RT-PCR)技术分析菜心PAL基因转录表达差异。鲜切菜心木质素含量随着贮藏时间增长呈上升趋势,在贮藏5 d后与对照组相比降低;PAL活性及其基因转录表达均呈现先下降后升高的趋势,在3 d时高氧组要低于对照组,5 d后差异不大。高氧气调包装能一定程度上抑制PAL基因的转录表达,降低PAL的活性,减少木质素的含量。
Explore the effect of high oxygen modified packaging(HOMP) on fresh cut cabbage lignified Phenylalaninammo-nialyase(PAL). HOMP(oxygen>90 %)was used to pack the fresh-cut Chinese cabbage with air packaged as control. The treated cabbage was stored at 25 ℃,then the lignin content and PAL activity of the cabbage were detected every day during five days of storage,and the transcriptional expression of PAL gene by reverse transcription-polymerase chain reaction(RT-PCR)was also analyzed in chinese cabbage. The experimental results showd that,fresh-cut cabbage lignin content increased with storage time,with the HOMP lower than the control at the end of storage. The activity of PAL and its gene transcriptional expression showed that a tendency was found to decrease first and then increase. At 3 days,the HOMP was lower than the control,but there was not much difference at the end of storage. Therefore,the HOMP could inhibit the transcriptional expression of PAL gene,and reduce the activity of PAL and the lignin content of fresh-cut cabbage.
Explore the effect of high oxygen modified packaging(HOMP) on fresh cut cabbage lignified Phenylalaninammo-nialyase(PAL). HOMP(oxygen>90 %)was used to pack the fresh-cut Chinese cabbage with air packaged as control. The treated cabbage was stored at 25 ℃,then the lignin content and PAL activity of the cabbage were detected every day during five days of storage,and the transcriptional expression of PAL gene by reverse transcription-polymerase chain reaction(RT-PCR)was also analyzed in chinese cabbage. The experimental results showd that,fresh-cut cabbage lignin content increased with storage time,with the HOMP lower than the control at the end of storage. The activity of PAL and its gene transcriptional expression showed that a tendency was found to decrease first and then increase. At 3 days,the HOMP was lower than the control,but there was not much difference at the end of storage. Therefore,the HOMP could inhibit the transcriptional expression of PAL gene,and reduce the activity of PAL and the lignin content of fresh-cut cabbage.
引文
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[14]陈学红,秦卫东,马利华,等,高氧气调包装对绿芦笋木质化的影响[J].食品科学,2009,30(22):350-353
[15]杜欣谊.苯丙氨酸解氨酶的研究进展[J].现代化农业,2016(7):24-26
[16]DIXON R A,PAIVA N L.Stress-Induced Phenylpropanoid Metabolism[J].The Plant cell,1995,7(7):1085-1097
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[24]An Jianshen,Zhang Min,Lu Qirui.Changes in some quality indexes in fresh~cut green asparagus pretreated with aqueous ozone and subsequent modified atmosphere packaging[J].Journal of Food Engineering,2007,78(2):340-344
[25]孙涵,王相友,李玲.高氧动态气调对双孢蘑菇细胞壁代谢及木质化进程的影响[J].食品科学,2018,39(11):255-262
[26]RAN Xiu-zhi,LIANG Ying,LI Jia-na.Analysis of the Lignin Contents and Related Enzymes Activities in Seed Coat Between BlackSeeded and Yellow-Seeded Rapes(Brassica napus L.)[J].中国农业科学:英文版,2005,4(12):890-897
[27]朱海英,李人圭,王隆华,等.丝瓜果实发育中木质素代谢及有关导管分化的生理生化研究[J].华东师范大学学报(自然科学版),1997(1):87-94
[28]吴晓丽,顾小平,苏梦云,等.离体毛竹笋纤维素和木质素含量及POD和PAL活性研究[J].林业科学研究,2008,21(5):697-701
[29]吴锦程,梁杰,陈建琴,等.采收成熟度与冷藏枇杷果实木质化关系研究[J].食品科学,2010,31(2):255-259
[30]周涛,许时婴.MAP对轻度加工茭白贮藏过程中生理变化的影响[J].食品科学,2006,27(10):564-567
[31]李伟丽,李喜宏,张培培,等.高氧处理对鲜切雪莲果货架寿命的影响[J].中国农学通报,2009,25(20):72-75
[32]吴绪敏,周颖越,欧阳杰.高氧气调包装鲜切洋葱的研究[J].食品科学,2007,28(9):564-567
[2]李凤玲,徐培洲,肖祎,等.植物凝集素及其在抗虫基因工程中的应用[J].安徽农业科学,2006,34(3):430-432,439
[3]Freudenberg K.Constitution and Biosynthesis of Lignin[J].Quarterly Review of Biology,1968,46(2):171-175
[4]Boerjan W,Ralph J,Baucher M.Lignin Biosynthesis[J].Annual review of plant biology,2003,54(1):519-546
[5]Douglas C J.Phenylpropanoid metabolism and lignin biosynthesis:From weeds to trees[J].Trends in Plant Science,1996,1(6):171-178
[6]Humphreys J M,Chapple C.Rewriting the lignin roadmap[J].Curr Opin Plant Biol,2002,5(3):224-229
[7]王贵禧,李鹏霞,梁丽松,等.高氧处理对冬枣货架期间膜脂过氧化和保护酶活性的影响[J].园艺学报,2006,33(3):609-612
[8]郑永华,苏新国,李欠盛,等.高氧对枇杷果实贮藏期间呼吸速率和多酚氧化酶活性及品质的影响(简报)[J].植物生理学通讯,2000,36(4):318-320
[9]Day B P F.High oxygen modified atmosphere packaging for fresh prepared produce[J].Postharv News info,1996,7(3):31-34
[10]Koukol J,Conn E E.The Metabolism of Aromatic Compounds in Higher Plants IV.PURIFICATION AND PROPERTIES OF THEPHENYLALANINE DEAMINASE OF HORDEUM VULGARE[J].Journal of Biological Chemistry,1961,236(10):12692
[11]Kirk T K,Obst J R.Lignin determination[J].Methods in enzymology,1988,161C:87-101
[12]Zhong R.Essential Role of Caffeoyl Coenzyme A O-Methyltransferase in Lignin Biosynthesis in Woody Poplar Plants[J].PLANT PHYSI-OLOGY,2000,124(2):563-578
[13]Bate N J,Orr J,Ni W,et al.Quantitative relationship between phenylalanine ammonia-lyase levels and phenylpropanoid accumulation in transgenic tobacco identifies a rate-determining step in natural product synthesis[J].Proceedings of the National Academy of Sciences,1994,91(16):7608-7612
[14]陈学红,秦卫东,马利华,等,高氧气调包装对绿芦笋木质化的影响[J].食品科学,2009,30(22):350-353
[15]杜欣谊.苯丙氨酸解氨酶的研究进展[J].现代化农业,2016(7):24-26
[16]DIXON R A,PAIVA N L.Stress-Induced Phenylpropanoid Metabolism[J].The Plant cell,1995,7(7):1085-1097
[17]Northcote K D H.Regulation of Induction of Phenylalanine Ammonia-Lyase in Suspension Cultures of Phaseolus vulgaris[J].Planta,1979,146(4):433-440
[18]Bolwell G P,Cramer C L,Lamb C J,et al.L-Phenylalanine ammonia-lyase fromPhaseolus vulgaris:Modulation of the levels of active enzyme bytrans-cinnamic acid[J].Planta,1986,169(1):97-107
[19]LEYVA A.Low temperature induces the accumulation of Phenylalanine ammonia lyase and chalcone synthase mRNA of Arabidopsis thaliana in a light dependent manner[J].Plant Physiology,1995,108(1):39-46
[20]刘卫红,程水源.光照及机械损伤对银杏叶苯丙氨酸解氨酶活性的影响[J].湖北农业科学,2003(3):73-74
[21]Tucker M L,Laties G G.The dual role of oxygen in avocado fruit respiration:Kinetic analysis and computer modeling of diffusion-affected respiratory oxygen isotherms[J].Plant Cell Environ,1995(8):117-127
[22]Limbo S,Piergiovanni L.Minimally processed potatoes:part2.Effects of high oxygen partial pressures in combination with ascorbic and citric acid on loss of some quality traits[J].Posthavest Biology and Technology,2007,43(2):221-229
[23]陈学红,秦卫东,马利华,等.高氧气调包装对鲜切莴苣呼吸和酶活性的影响[J].食品与发酵工业,2011,37(12):208-212
[24]An Jianshen,Zhang Min,Lu Qirui.Changes in some quality indexes in fresh~cut green asparagus pretreated with aqueous ozone and subsequent modified atmosphere packaging[J].Journal of Food Engineering,2007,78(2):340-344
[25]孙涵,王相友,李玲.高氧动态气调对双孢蘑菇细胞壁代谢及木质化进程的影响[J].食品科学,2018,39(11):255-262
[26]RAN Xiu-zhi,LIANG Ying,LI Jia-na.Analysis of the Lignin Contents and Related Enzymes Activities in Seed Coat Between BlackSeeded and Yellow-Seeded Rapes(Brassica napus L.)[J].中国农业科学:英文版,2005,4(12):890-897
[27]朱海英,李人圭,王隆华,等.丝瓜果实发育中木质素代谢及有关导管分化的生理生化研究[J].华东师范大学学报(自然科学版),1997(1):87-94
[28]吴晓丽,顾小平,苏梦云,等.离体毛竹笋纤维素和木质素含量及POD和PAL活性研究[J].林业科学研究,2008,21(5):697-701
[29]吴锦程,梁杰,陈建琴,等.采收成熟度与冷藏枇杷果实木质化关系研究[J].食品科学,2010,31(2):255-259
[30]周涛,许时婴.MAP对轻度加工茭白贮藏过程中生理变化的影响[J].食品科学,2006,27(10):564-567
[31]李伟丽,李喜宏,张培培,等.高氧处理对鲜切雪莲果货架寿命的影响[J].中国农学通报,2009,25(20):72-75
[32]吴绪敏,周颖越,欧阳杰.高氧气调包装鲜切洋葱的研究[J].食品科学,2007,28(9):564-567
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