胡萝卜黑斑病抗性的动态遗传分析
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摘要
采用加性-显性遗传模型,利用19份橘色胡萝卜试材配制63个杂交组合,研究3个发病时期下黑斑病抗性的动态遗传规律。结果表明,各时期的遗传效应以加性效应为主,在发病中期和晚期(V_A=0.656和0.551)达到显著水平,并且在发病中期显著增加,说明该时期是挖掘相关抗病基因的适宜时期;显性效应在发病晚期显著增加,达到最高值(V_D=0.311)。黑斑病抗性存在显著的狭义遗传率和广义遗传率,最高为0.574和0.827,说明通过选择抗性亲本可以提高后代的抗病性。获得5份可以显著增加后代黑斑病抗性的亲本材料P12、P14、P15、P16、P17。
Taking additive-dominance genetic model,and using 19 orange carrot lines as experiment material to prepare their 63 F_1 crosses,this paper studied the dynamic genetic law of Alternaria leaf blight resistance during 3 stages.The results indicated that the additive effect was the major genetic effect,which reached significant level during median and late stages(V_A=0.656 and 0.551)and increased significantly in median stage,indicating that this stage was the suitable time to study related resistant genes.The dominance effect was increased significantly and reached the highest value(V_D=0.311)in late stage.There were narrow heritability and broad heritability existed in Alternaria leaf blight resistence.The highest were 0.574 and 0.827,indicating that selecting resistant parent could improve disease resistance in offspring.Five parent materials,which could significantly improve Alternaria leaf blight resistence in offspring,were obtained.They were P12,P14,P15,P16,P17.
Taking additive-dominance genetic model,and using 19 orange carrot lines as experiment material to prepare their 63 F_1 crosses,this paper studied the dynamic genetic law of Alternaria leaf blight resistance during 3 stages.The results indicated that the additive effect was the major genetic effect,which reached significant level during median and late stages(V_A=0.656 and 0.551)and increased significantly in median stage,indicating that this stage was the suitable time to study related resistant genes.The dominance effect was increased significantly and reached the highest value(V_D=0.311)in late stage.There were narrow heritability and broad heritability existed in Alternaria leaf blight resistence.The highest were 0.574 and 0.827,indicating that selecting resistant parent could improve disease resistance in offspring.Five parent materials,which could significantly improve Alternaria leaf blight resistence in offspring,were obtained.They were P12,P14,P15,P16,P17.
引文
曹琼文,付玲,李成江,欧承刚,刘波,赵志伟,任怀富,庄飞云.2018.胡萝卜种质资源的农艺性状及黑斑病抗性研究.中国蔬菜,(11):53-59.
陈幼玉,祁建民,林荔辉,梁康迳,周瑞阳,吴建梅.2005.红麻株高与茎粗性状的动态发育遗传分析.应用生态学报,16(6):1011-1016.
方智远.2017.中国蔬菜育种学.北京:中国农业出版社.
欧承刚,庄飞云,赵志伟,黄建新.2009.胡萝卜主要病害及抗病育种研究进展.中国蔬菜,(4):1-6.
石春海,吴建国,樊龙江,朱军,吴平.2002.不同环境条件下稻米透明度的发育遗传分析.遗传学报,29(1):56-61.
朱军.1997.遗传模型分析方法.北京:中国农业出版社.
左清凡,朱军,刘宜柏,潘晓云,张建中.2000.非等试验设计植株农艺及产量性状的数量遗传分析方法.中国农业科学,33(2):30-33.
Boedo C,Berruyer R,Lecomte M,Bersihand S,Briard M,le Clerc V,Simoneau P,Poupard P.2010.Evaluation of different methods for the characterization of carrot resistance to the Alternaria leaf blight pathogen(Alternaria dauci)revealed two qualitatively different resistances.Plant Pathology,59:368-375.
Boedo C,Benichou S,Berruyer R,Bersihand S,Dongo A,Simoneau P,Lecomte M,Briard M,le Clerc V,Poupard P.2012.Evaluating aggressiveness and host range of Alternaria dauci in a controlled environment.Plant Pathology,61:63-75.
Boiteux L S,della Vecchia P T,Reifschneider J B.1993.Heritability estimate for resistance to Alternaria dauci in carrot.Plant Breeding,110:165-167.
Chen G B,Zhu Z X,Zhang F T,Zhu J.2012.Quantitative genetic analysis station for the genetic analysis of complex traits.Chinese Science Bulletin,57(21):2721-2726.
Corbaz R,Perko J.1995.Observations sur le degréde sensibilitéàl’alternariose du feuillage(Alternaria dauci)des variétés de carottes de garde.Revue Suisse Viticulture Arboriculture Horticulture,27:311-312.
le Clerc V,Pawelec A,Birolleau-Touchard C,Suel A,Briard M.2009.Genetic architecture of factors underlying partial resistance to Alternaria leaf blight in carrot.Theoretical and Applied Genetics,118(7):1251-1259.
le Clerc V,Marques S,Suel A,Huet S,Hamama L,Voisine L,Auperpin E,Jourdan M,Barrot L,Prieur R,Briard M.2015.QTL mapping of carrot resistance to leaf blight with connected populations:stability across years and consequences for breeding.Theoretical and Applied Genetics,128(11):2177-2187.
Li T G,Zhang D D,Zhou L,Kong Z Q,Hussaini A S,Wang D,Li J J,Short D,Dhar N,Klosterman S J,Wang B L,Yin C M,Subbarao K V,Chen J Y,Dai X F.2018.Genomewide identification and functional analyses of the CRK gene family in cotton reveals GbCRK18 confers Verticillium wilt resistance in Gossypium barbadense.Front Plant Sci,9:1266.doi:10.3389/fpls.2018.01266.
Neik T X,Barbetti M J,Batley J.2017.Current status and challenges in identifying disease resistance genes in Brassica napus.Front Plant Sci,8:1788.doi:10.3389/fpls.2017.01788.
Pawelec A,Dubourg C,Briard M.2006.Evaluation of carrot resistance to Alternaria leaf blight in controlled environments.Plant Pathology,55:68-72.
Pryor B M,Strandberg J O,Davis R M,Nunez J J,Gilbertson R L.2002.Survival and persistence of Alternaria dauci in carrot cropping systems.Plant Disease,86:1115-1122.
Simon P W,Strandberg J O.1998.Diallel analysis of resistance in carrot to Alternaria leaf blight.Journal of the American Society for Horticultural Science,123:412-415.
Strandberg J O,Bassett M J,Peterson C E,Berger R D.1972.Source of resistance to Alternaria dauci.Hort Science,7:345.
Vieira V J,Dias Casali V W,Milagres J C,Cardoso A A,Regazzi A J.1991.Heritability and genetic gain for resistance to leaf blight in carrot(Daucus carota L.)populations evaluated at different times after sowing.Revista Brazilian de Genetica,14:501-508.
Vintal H,Ben-Noon E,Shlevin E,Yermiyahu U,Shtienberg D,Dinoor A.1999.Influence of rate of soil fertilization on Alternaria leaf blight(Alternaria dauci)in carrots.Phytoparasitica,7:1-8.
Wang L,Hu X H,Lin G,Zhao D M,Shi J,Zhao Z X,Zeng R,Li H J,Li D Q,Fan J,Li Y,Huang F,Wang W M.2017.Expression-based genotyping of the rice blast resistance genes in the elite maintainer line Yixiang1B.Eur J Plant Pathol,148:955-965.
Xu L,Henke M,Zhu J,Kurth W,Buck-Sorlin G.2011.Afunctional-structural model of rice linking quantitative genetic information with morphological development and physiological processes.Annals of Botany,107(5):817-828.
Zhu J.1995.Analysis of conditional genetic effects and variance components in developmental genetics.Genetics,141:1633-1639.
陈幼玉,祁建民,林荔辉,梁康迳,周瑞阳,吴建梅.2005.红麻株高与茎粗性状的动态发育遗传分析.应用生态学报,16(6):1011-1016.
方智远.2017.中国蔬菜育种学.北京:中国农业出版社.
欧承刚,庄飞云,赵志伟,黄建新.2009.胡萝卜主要病害及抗病育种研究进展.中国蔬菜,(4):1-6.
石春海,吴建国,樊龙江,朱军,吴平.2002.不同环境条件下稻米透明度的发育遗传分析.遗传学报,29(1):56-61.
朱军.1997.遗传模型分析方法.北京:中国农业出版社.
左清凡,朱军,刘宜柏,潘晓云,张建中.2000.非等试验设计植株农艺及产量性状的数量遗传分析方法.中国农业科学,33(2):30-33.
Boedo C,Berruyer R,Lecomte M,Bersihand S,Briard M,le Clerc V,Simoneau P,Poupard P.2010.Evaluation of different methods for the characterization of carrot resistance to the Alternaria leaf blight pathogen(Alternaria dauci)revealed two qualitatively different resistances.Plant Pathology,59:368-375.
Boedo C,Benichou S,Berruyer R,Bersihand S,Dongo A,Simoneau P,Lecomte M,Briard M,le Clerc V,Poupard P.2012.Evaluating aggressiveness and host range of Alternaria dauci in a controlled environment.Plant Pathology,61:63-75.
Boiteux L S,della Vecchia P T,Reifschneider J B.1993.Heritability estimate for resistance to Alternaria dauci in carrot.Plant Breeding,110:165-167.
Chen G B,Zhu Z X,Zhang F T,Zhu J.2012.Quantitative genetic analysis station for the genetic analysis of complex traits.Chinese Science Bulletin,57(21):2721-2726.
Corbaz R,Perko J.1995.Observations sur le degréde sensibilitéàl’alternariose du feuillage(Alternaria dauci)des variétés de carottes de garde.Revue Suisse Viticulture Arboriculture Horticulture,27:311-312.
le Clerc V,Pawelec A,Birolleau-Touchard C,Suel A,Briard M.2009.Genetic architecture of factors underlying partial resistance to Alternaria leaf blight in carrot.Theoretical and Applied Genetics,118(7):1251-1259.
le Clerc V,Marques S,Suel A,Huet S,Hamama L,Voisine L,Auperpin E,Jourdan M,Barrot L,Prieur R,Briard M.2015.QTL mapping of carrot resistance to leaf blight with connected populations:stability across years and consequences for breeding.Theoretical and Applied Genetics,128(11):2177-2187.
Li T G,Zhang D D,Zhou L,Kong Z Q,Hussaini A S,Wang D,Li J J,Short D,Dhar N,Klosterman S J,Wang B L,Yin C M,Subbarao K V,Chen J Y,Dai X F.2018.Genomewide identification and functional analyses of the CRK gene family in cotton reveals GbCRK18 confers Verticillium wilt resistance in Gossypium barbadense.Front Plant Sci,9:1266.doi:10.3389/fpls.2018.01266.
Neik T X,Barbetti M J,Batley J.2017.Current status and challenges in identifying disease resistance genes in Brassica napus.Front Plant Sci,8:1788.doi:10.3389/fpls.2017.01788.
Pawelec A,Dubourg C,Briard M.2006.Evaluation of carrot resistance to Alternaria leaf blight in controlled environments.Plant Pathology,55:68-72.
Pryor B M,Strandberg J O,Davis R M,Nunez J J,Gilbertson R L.2002.Survival and persistence of Alternaria dauci in carrot cropping systems.Plant Disease,86:1115-1122.
Simon P W,Strandberg J O.1998.Diallel analysis of resistance in carrot to Alternaria leaf blight.Journal of the American Society for Horticultural Science,123:412-415.
Strandberg J O,Bassett M J,Peterson C E,Berger R D.1972.Source of resistance to Alternaria dauci.Hort Science,7:345.
Vieira V J,Dias Casali V W,Milagres J C,Cardoso A A,Regazzi A J.1991.Heritability and genetic gain for resistance to leaf blight in carrot(Daucus carota L.)populations evaluated at different times after sowing.Revista Brazilian de Genetica,14:501-508.
Vintal H,Ben-Noon E,Shlevin E,Yermiyahu U,Shtienberg D,Dinoor A.1999.Influence of rate of soil fertilization on Alternaria leaf blight(Alternaria dauci)in carrots.Phytoparasitica,7:1-8.
Wang L,Hu X H,Lin G,Zhao D M,Shi J,Zhao Z X,Zeng R,Li H J,Li D Q,Fan J,Li Y,Huang F,Wang W M.2017.Expression-based genotyping of the rice blast resistance genes in the elite maintainer line Yixiang1B.Eur J Plant Pathol,148:955-965.
Xu L,Henke M,Zhu J,Kurth W,Buck-Sorlin G.2011.Afunctional-structural model of rice linking quantitative genetic information with morphological development and physiological processes.Annals of Botany,107(5):817-828.
Zhu J.1995.Analysis of conditional genetic effects and variance components in developmental genetics.Genetics,141:1633-1639.
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