外源性十溴联苯醚暴露对小鼠早期胚胎发育的影响
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摘要
目的:探讨外源性十溴联苯醚(BDE-209)暴露对小鼠早期胚胎发育的影响。方法:选择健康昆明雌性小鼠40只、雄性小鼠10只。40只雌小鼠随机分成4组:BDE-209低剂量组(100 mg/kg)、BDE-209中剂量组(300 mg/kg)、BDE-209高剂量组(500mg/kg)和对照组,每组10只。所有雌小鼠采用灌胃方式进行BDE-209染毒,对照组给予等量生理盐水。观察比较各组体外受精(IVF)情况及卵泡液中肿瘤坏死因子-α(TNF-α)和干扰素-γ(IFN-γ)浓度。结果:BDE-209中剂量组及高剂量组受精率明显低于对照组(P<0.05),但BDE-209低剂量组与对照组比较差异无统计学意义(P>0.05)。与对照组相比,BDE-209低、中、高剂量组4-细胞、8-细胞、桑椹胚及囊胚形成率均明显下降,卵泡液中TNF-α及IFN-γ浓度均明显升高(P<0.05),且随着BDE-209暴露剂量的增加呈降低/升高趋势,不同剂量组间比较差异有统计学意义(P<0.05)。结论:外源性BDE-209暴露会减少小鼠IVF的获卵率,降低受精率,对小鼠早期胚胎发育产生负性影响,且呈剂量依赖性。
Objective:To investigate the effect of exogenous BDE-209 exposure on early embryonic development in mice.Methods:40 female mice were randomly divided into 4 groups:low-dose(100 mg/kg),middle-dose(300 mg/kg),and high-dose(500 mg/kg)BDE-209 groups and control group,with 10 mice in each group.Mice were administered different doses of BDE-209 by gavage except for the controls(received equal volume of saline).The in vivo fertilization(IVF)status,and the levels of tumor necrosis factor(TNF)-αand interferon(IFN)-γin follicular fluid were observed and compared.Results:The fertilization rate in highdose and middle-dose BDE-209 groups was significantly lower than that in the control group(P<0.05),while no significant difference was found between the low-dose group and the control group(P >0.05).Compared with the control group,the 4-to 8-cell embryos and blastocyst formation rate were reduced,and while the levels of TNF-αand IFN-γin follicular fluid were elevated in the BDE-209-treated groups(P<0.05).Conclusion:BDE-209 exposure could result in decreased fertility rate and blastocyst formation rate in mice,and was dose-dependent.
Objective:To investigate the effect of exogenous BDE-209 exposure on early embryonic development in mice.Methods:40 female mice were randomly divided into 4 groups:low-dose(100 mg/kg),middle-dose(300 mg/kg),and high-dose(500 mg/kg)BDE-209 groups and control group,with 10 mice in each group.Mice were administered different doses of BDE-209 by gavage except for the controls(received equal volume of saline).The in vivo fertilization(IVF)status,and the levels of tumor necrosis factor(TNF)-αand interferon(IFN)-γin follicular fluid were observed and compared.Results:The fertilization rate in highdose and middle-dose BDE-209 groups was significantly lower than that in the control group(P<0.05),while no significant difference was found between the low-dose group and the control group(P >0.05).Compared with the control group,the 4-to 8-cell embryos and blastocyst formation rate were reduced,and while the levels of TNF-αand IFN-γin follicular fluid were elevated in the BDE-209-treated groups(P<0.05).Conclusion:BDE-209 exposure could result in decreased fertility rate and blastocyst formation rate in mice,and was dose-dependent.
引文
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[10]李秋华,祝晓丽,魏思达,等.PM2.5暴露对小鼠体外受精及胚胎发育的影响[J].实用医学杂志,2017,33(20):3372-3375.
[2]杜丽丽,匡丽云,陈敦金.BDE-209暴露对人胚胎干细胞印记基因表达的影响[J].环境与健康杂志,2015,32(7):571-574.
[3]刘畅,朱春艳,钱波,等.十溴联苯醚(BDE-209)孕哺期及出生后暴露对子代小鼠血液系统影响初探[J].生态毒理学报,2016,11(2):315-324.
[4]BARON E,DISSANAYAKE A,VILA-CANO J,et al.Evaluation of thegenotoxic and physiological effects of decabromodiphenyl ether(BDE-209)and dechlorane plus(DP)flame retardants in marine mussels(Mytilus galloprovincialis)[J].Environmental Science&Technology,2016,50(5):2700.
[5]王志新,段华英,王玲,等.十溴联苯醚对小鼠受精卵发育的影响[J].中山大学学报,2011,32(1):52-55.
[6]COSTA L G,DE LAAT R,TAGLIAFERRI S,et al.A mechanistic view of polybrominated diphenyl ether(PBDE)developmental neurotoxicity[J].Toxicol Lett,2014,230(2):282-294.
[7]刘婷.内质网应激通路在十溴联苯醚致小鼠海马神经元细胞系HT-22细胞凋亡中作用[D].安徽医科大学,2014.
[8]王秀霞,王天任,易晓芳,等.卵泡液中TNF-α和IFN-γ与卵丘颗粒细胞凋亡关系的探讨[J].生殖与避孕,2010,30(8):519-522.
[9]QUIRK S M,PORTER D A,HUBER S C,et al.Potentiation of Fasmediated apoptosis of murine granulosa cells by interferon,tumor necrosis factor and cycloheximide[J].Endocrinology,1998,139(12):4860-4869.
[10]李秋华,祝晓丽,魏思达,等.PM2.5暴露对小鼠体外受精及胚胎发育的影响[J].实用医学杂志,2017,33(20):3372-3375.