脑心通胶囊肠吸收液清除DPPH自由基的有效成分辨识研究
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摘要
目的:以肠吸收液-体外活性相结合,并利用拆方重组法研究脑心通胶囊中发挥抗氧化作用的单味或多味中药。方法:应用外翻肠囊法制备肠吸收液,同时拆方或构建重组方,生化试剂盒结合酶标仪检测DPPH自由基的清除率,寻找脑心通胶囊组方中抗氧化作用的单味或多味中药。结果:丹参、黄芪和赤芍的抗氧化作用显著高于脑心通胶囊组,将此三味药组合成三味复方,同时从全方中去除上述三味药,重组成去三味复方,均按原方配比组合,制备肠吸收液,结果显示,三味复方组仍具有较强的抗氧化活性,而去三味组方的抗氧化活性显著降低。当单味药的质量浓度在单味药组、三味复方组及脑心通胶囊组中的质量浓度相同时,三味复方组活性显著低于脑心通胶囊组,与三味药单独作用比较,也明显下降。结论:黄芪、丹参和赤芍是脑心通胶囊抗氧化作用的主要活性成分,但脑心通胶囊抗氧化作用仍是全方的综合作用结果。
Objective:The study aims at finding components in Naoxintong capsule through separation and combination of the prescription with intestinal absorption liquid-activity in vitro as mode of administration.Methods:Naoxintong intestinal absorption liquids of single,combination and total prescription were prepared.DPPH scavenging activity was measured by biochemical tests and a microplate reader.One or more of traditional Chinese medicine in Naoxintong capsule which displayed antioxidant activity were screened through separation and combination of prescription.Results:Antioxidant activity of Radix Salviae Miltiorrhizae,Rstragali Radix and Radix Paeoniae Rubra were stronger than that of Naoxintong capsule.Combined these three kinds of Chinese medicines into one prescription,meanwhile,combined the remained Chinese medicine into the other prescription,the proportion of every ingredient was consistent with original prescription,and then intestinal absorption liquid in each group was prepared.Results showed that antioxidant activity of prescription with the three was the strongest,and antioxidant activity of prescription without the three was quite low.When the concentration of any kind of Chinese medicine in the above three was identical with the concentration in the prescription and single herb,Naoxintong capsule showed the best antioxidant activity,and single herb group took the second place.Conclusion:Radix Salviae Miltiorrhizae,Rstragali Radix and Radix Paeoniae Rubra are the main components exerting antioxidant activities in Naoxintong capsule,but antioxidant activity of Naoxintong capsule is the comprehensive effect of whole herb in the prescription.
Objective:The study aims at finding components in Naoxintong capsule through separation and combination of the prescription with intestinal absorption liquid-activity in vitro as mode of administration.Methods:Naoxintong intestinal absorption liquids of single,combination and total prescription were prepared.DPPH scavenging activity was measured by biochemical tests and a microplate reader.One or more of traditional Chinese medicine in Naoxintong capsule which displayed antioxidant activity were screened through separation and combination of prescription.Results:Antioxidant activity of Radix Salviae Miltiorrhizae,Rstragali Radix and Radix Paeoniae Rubra were stronger than that of Naoxintong capsule.Combined these three kinds of Chinese medicines into one prescription,meanwhile,combined the remained Chinese medicine into the other prescription,the proportion of every ingredient was consistent with original prescription,and then intestinal absorption liquid in each group was prepared.Results showed that antioxidant activity of prescription with the three was the strongest,and antioxidant activity of prescription without the three was quite low.When the concentration of any kind of Chinese medicine in the above three was identical with the concentration in the prescription and single herb,Naoxintong capsule showed the best antioxidant activity,and single herb group took the second place.Conclusion:Radix Salviae Miltiorrhizae,Rstragali Radix and Radix Paeoniae Rubra are the main components exerting antioxidant activities in Naoxintong capsule,but antioxidant activity of Naoxintong capsule is the comprehensive effect of whole herb in the prescription.
引文
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[14] 黄斌,陈晓萌,张迎春,等.元胡止痛方肠吸收液对大鼠离体胸主动脉环张力的影响[J].中国实验方剂学杂志,2012,18(5):117-120.
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[20] GAWRON-SKARBEK A,CHRZCZANOWICZ J,KOSTKA J,et al.Physical Activity,Aerobic Capacity,and Total Antioxidant Capacity in Healthy Men and in Men with Coronary Heart Disease[J/OL].Oxid Med Cell Longev,2015.http://dx.doi.org/10.1155/2015/197307.doi:10.1155/2015/197307.
[21] GAWRON-SKARBEK A,CHRZCZANOWICZ J,KOSTKA J,et al.Cardiovascular risk factors and total serum antioxidant capacity in healthy men and in men with coronary heart disease[J/OL].Biomed Res Int,2014.https://www.hindawi.com/journals/bmri/2014/216964/.doi:10.1155/2014/216964.
[22] 马丙祥,董宠凯.丹参的药理作用研究新进展[J].中国药房,2014,25(7):663-665.
[23] 张鑫,杨英杰,吕庆章.黄芪异黄酮类化合物抗氧化活性的密度泛函理论研究[J].化学研究与应用,2012,24(11):1662-1669.
[24] 黄海兰,王国明,徐波.赤芍抗氧化活性及其成分研究[J].食品科学,2007,28(7):76-77.
[2] 王均宁,龙子江,王钦茂.真武汤及其拆方强心利尿作用的实验研究[J].中成药,1997,19(3):27-29.
[3] 赵涛,赵步长,伍海勤,等.脑心通胶囊在心脑血管病中的作用研究[J].中医杂志,2012,53(24):2150-2152.
[4] 孔德梅.脑心通胶囊对冠心病患者血液流变的影响[J].药物与临床,2009,17(9):788-789.
[5] 邓常青,葛金文,胡俊.益气活血法抗脑缺血再灌注损伤机理的实验研究Ⅰ—益气活血药物对氧自由基所致大鼠脑组织损伤的影响[J].湖南中医学院院报,1994,14(2):38-41.
[6] 马静,龙铟,王宗仁,等.益气活血复方对心肌线粒体氧自由基损伤的保护作用[J].心脏杂志,2006,18(2):178-181.
[7] 童荣生.自由基与心血管疾病[J].现代临床医学,2007,33(增刊2):188-190.
[8] AZIZOVA O A,SERGIENKO V I,SYRKIN A L,et al.Clinical and prognostic significance of free radical processes in patients with coronary heart disease[J].Vestn Ross Akad Med Nauk,2009,10:32-40.
[9] HANASSOULIS G,BROPHY J M,RICHARD H,et al.Gout,allopurinol use and heart failure outcomes[J].Arch Intern Med,2010,170:1358-1364.
[10] ZHANG F B,HUANG B,ZHAO Y,et al.BNC Protects H9c2 Cardiomyoblasts from H2O2-Induced Oxidative Injury through ERK1/2 Signaling Pathway[J/OL].Evid Based Complement Alternat Med,2013.http://dx.doi.org/10.1155/2013/802784.doi:10.1155/2013/802784.
[11] 宋怀恩,闻韧.抗氧化剂筛选方法的研究进展[J].中国药物化学杂志,2003,13(2):119-122.
[12] 谭琴,朱晶晶,王维皓,等.肠外翻技术快速发现车前子吸收成分群的研究[J].中国中药杂志,2011,36(2):150-153.
[13] 陈晓鹏,李昂,褚扬,等.养血清脑颗粒中有效成分的肠外翻吸收研究[J].中国实验方剂学杂志,2013,19(4):159-162.
[14] 黄斌,陈晓萌,张迎春,等.元胡止痛方肠吸收液对大鼠离体胸主动脉环张力的影响[J].中国实验方剂学杂志,2012,18(5):117-120.
[15] ZHANG Y C,XU H Y,CHEN X M,et al.Study on the application of intestinal absorption in vitro coupled with bioactivity assessment in Yuanhu Zhitong preparation[J].J Med Plants Res,2012,6(10):1941-1947.
[16] 何子龙,方文娟,张方博,等.疏风解毒胶囊肠吸收液对LPS诱导巨噬细胞释放细胞因子的影响[J],中国现代中药,2015,17(4):345-348.
[17] DORN II G W.Apoptotic and non-apoptotic programmed cardiomyocyte death in ventricular remodeling[J].Cardiovasc Res,2009,81(3):465-473.
[18] BUTLER T L,AU C G,YANG B,et al.Cardiac aquaporin expression in humans,rats,and mice[J].Am J Physiol Heart Circ Physiol,2006,291(2):H705-H713.
[19] MORALES D,PIRIS A J,RUIZ-RODRIGUEZ A,et al.Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method[J].Biotechnol Prog,2018,34(3):744-765.
[20] GAWRON-SKARBEK A,CHRZCZANOWICZ J,KOSTKA J,et al.Physical Activity,Aerobic Capacity,and Total Antioxidant Capacity in Healthy Men and in Men with Coronary Heart Disease[J/OL].Oxid Med Cell Longev,2015.http://dx.doi.org/10.1155/2015/197307.doi:10.1155/2015/197307.
[21] GAWRON-SKARBEK A,CHRZCZANOWICZ J,KOSTKA J,et al.Cardiovascular risk factors and total serum antioxidant capacity in healthy men and in men with coronary heart disease[J/OL].Biomed Res Int,2014.https://www.hindawi.com/journals/bmri/2014/216964/.doi:10.1155/2014/216964.
[22] 马丙祥,董宠凯.丹参的药理作用研究新进展[J].中国药房,2014,25(7):663-665.
[23] 张鑫,杨英杰,吕庆章.黄芪异黄酮类化合物抗氧化活性的密度泛函理论研究[J].化学研究与应用,2012,24(11):1662-1669.
[24] 黄海兰,王国明,徐波.赤芍抗氧化活性及其成分研究[J].食品科学,2007,28(7):76-77.