外周血中clusterin和septin-9及CA125在卵巢癌患者中的表达及其意义
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摘要
目的探讨卵巢癌患者外周血中clusterin和septin-9及CA125的表达水平及其临床意义。方法选择明确诊断为卵巢癌的患者62例为观察组,另选取健康体检者31例为对照组。分别抽取两组患者外周血,采用qRT-PCR方法检测两组患者clusterin和septin-9mRNA表达水平;采用ELISA法检测两组患者clusterin和septin-9蛋白表达水平;采用化学发光法检测两组患者糖类抗原-125(CA125)水平。比较观察组患者中不同病理分期、是否转移及不同分化程度患者间clusterin和septin-9及CA125等指标表达差异。结果与对照组相比,观察组患者外周血中clusterin和septin-9Mrna/蛋白表达量均显著升高,差异有统计学意义(P<0.05);与对照组相比,观察组患者外周血中CA125表达量显著升高,差异有统计学意义(P<0.05);Ⅰ～Ⅱ期卵巢癌患者与Ⅲ～Ⅳ期卵巢癌患者相比,clusterin基因/蛋白及CA125水平显著降低(P<0.05);与发生远处转移(包括淋巴结转移)者比较,未发生远处转移(包括淋巴结转移)者其septin-9基因/蛋白表达水平显著降低(P<0.05);不同分化程度患者间clusterin、septin-9及CA125表达水平均未见明显差异。结论卵巢癌患者外周血中clusterin、septin-9及CA125水平较正常人群均显著升高,其中clusterin和CA125表达增高者可能提示病理分期较高,septin-9表达增高考虑存在原发病的远处转移可能。
Objective To detect the expression of clusterin,septin-9and CA125 in peripheral blood and its significance in ovarian cancer patients.Methods 62 cases of ovarian cancer were selected as the observation group,and 31 of the healthy persons were selected as the control group.Peripheral blood was extracted from two groups of patients.The expression levels of clusterin and septin-9mRNA in two groups were detected by qRT-PCR method.The expression level of clusterin and septin-9protein in two groups was detected by ELISA method,and CA125 level in two groups was detected by chemiluminescence.The differences in the expression of clusterin,septin-9and CA125 among the patients with different pathological stages,metastasis and different degree of differentiation were also compared.Results Compared with the control group,the observation group in the peripheral blood of patients with clusterin septin-9and Mrna/protein expression were significantly increased,there were statistical significantly differences(P<0.05).Compared with the control group,the expression levels of CA125 in peripheral blood of patients with the observation group increased significantly,there were statistical significantly differences(P <0.05).Compared toⅠ～Ⅱovarian cancer patients with stageⅢ～Ⅳovarian cancer,clusterin gene/protein and CA125 level were significantly decreased(P <0.05).There were no distant metastasis(including lymph node metastasis)than distant metastasis(including lymph node metastasis),the septin-9gene/protein expression level decreased significantly(P<0.05).The different degree of differentiation between patients with clusterin,septin-9and CA125 expression and there were no statistical significantly differences.Conclusion The levels of clusterin,septin-9and CA125 in the peripheral blood of ovarian cancer patients were significantly higher than those in the normal population.The higher expression of clusterin and CA125 may indicate higher pathological stage,higher expression of septin-9,and the possibility of distant metastasis of primary disease.
Objective To detect the expression of clusterin,septin-9and CA125 in peripheral blood and its significance in ovarian cancer patients.Methods 62 cases of ovarian cancer were selected as the observation group,and 31 of the healthy persons were selected as the control group.Peripheral blood was extracted from two groups of patients.The expression levels of clusterin and septin-9mRNA in two groups were detected by qRT-PCR method.The expression level of clusterin and septin-9protein in two groups was detected by ELISA method,and CA125 level in two groups was detected by chemiluminescence.The differences in the expression of clusterin,septin-9and CA125 among the patients with different pathological stages,metastasis and different degree of differentiation were also compared.Results Compared with the control group,the observation group in the peripheral blood of patients with clusterin septin-9and Mrna/protein expression were significantly increased,there were statistical significantly differences(P<0.05).Compared with the control group,the expression levels of CA125 in peripheral blood of patients with the observation group increased significantly,there were statistical significantly differences(P <0.05).Compared toⅠ～Ⅱovarian cancer patients with stageⅢ～Ⅳovarian cancer,clusterin gene/protein and CA125 level were significantly decreased(P <0.05).There were no distant metastasis(including lymph node metastasis)than distant metastasis(including lymph node metastasis),the septin-9gene/protein expression level decreased significantly(P<0.05).The different degree of differentiation between patients with clusterin,septin-9and CA125 expression and there were no statistical significantly differences.Conclusion The levels of clusterin,septin-9and CA125 in the peripheral blood of ovarian cancer patients were significantly higher than those in the normal population.The higher expression of clusterin and CA125 may indicate higher pathological stage,higher expression of septin-9,and the possibility of distant metastasis of primary disease.
引文
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[12] Gomaa W,Al-Ahwal M,Al-Maghrabi H,et al.Expression of clusterin in colorectal carcinoma in relation to clinicopathological criteria[J].Malays J Pathol,2017,39(3):243-250.
[13] Gregory JM,Whiten DR,Brown RA,et al.Clusterin protects neurons against intracellular proteotoxicity[J].ActaNeuropathol Commun,2017,5(1):81.
[14] Tsiolaki PL,Nastou KC,Louros NN,et al.Exploring amyloidogenicity of clusterin:a structural and bioinformatics analysis[J].Adv Exp Med Biol,2017,989:93-107.
[15] Jin R,Chen X,Han D,et al.Clusterin modulates transdifferentiation of non-small-cell lung cancer[J].BMC Cancer,2017,17(1):661.
[16]臧晔.细胞减灭术联合腹腔灌注热化疗对卵巢癌患者免疫功能及血浆clusterin、septin-9蛋白表达的影响[J].中国妇幼保健,2018,33(03):674-677.
[17] Zheng W,Yao M,Qian Q,et al.Oncogenic secretory clusterin in hepatocellular carcinoma:Expression at early staging and emerging molecular target[J].Oncotarget,2017,8(32):52321-52332.
[18] Mustafi S,Sant DW,Liu ZJ,et al.Ascorbate induces apoptosis in melanoma cells by suppressing Clusterin expression[J].Sci Rep,2017,7(1):3671.
[2]刘娟,冀静,邢兰英.Clusterin、E-cadherin在宫颈鳞癌组织中的表达及其相关性分析[J].西安交通大学学报:医学版,2017,81(06):904-907.
[3] Golan M,Mabjeesh NJ.Imaging of hypoxia-inducible factor 1alpha and septin 9interaction by bimolecular fluorescence complementation in live cancer cells[J].Oncotarget,2017,8(19):31830-31841.
[4] Walankiewicz M,Grywalska E,Polak G,et al.Myeloidderived suppressor cells in ovarian cancer:friend or foe?[J].Cent Eur J Immunol,2017,42(4):383-389.
[5] Lee CL,Kusunoki S,Huang CY,et al.Surgical and survival outcomes of laparoscopic staging surgery for patients with stage I ovarian cancer[J].Taiwan J Obstet Gynecol,2018,57(1):7-12.
[6] Bharti SK,Wildes F,Hung CF,et al.Metabolomic characterization of experimental ovarian cancer ascitic fluid[J].Metabolomics,2017,13(5):336-341.
[7]司晓辉,孙攀兴.Septin-9在子宫内膜癌组织中的表达及意义[J].中国妇幼健康研究,2017,28(02):140-141.
[8] Akil A,Peng J,Omrane M,et al.Septin 9induces lipid droplets growth by aphosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV[J].Nat Commun,2016,7:12203.
[9] Molnar B,Toth K,Bartak BK,et al.Plasma methylated septin 9:a colorectal cancer screening marker[J].Expert Rev Mol Diagn,2015,15(2):171-184.
[10]申华峰,耿嘉男,李景贺,等.Clusterin在肺癌中的表达及意义[J].中国老年学杂志,2014,34(12):3322-3323.
[11]黄秀英,耿嘉男,刘凯,等.Clusterin在UUO大鼠模型中的表达及诊断意义[J].中国实验诊断学,2014,18(10):1601-1602.
[12] Gomaa W,Al-Ahwal M,Al-Maghrabi H,et al.Expression of clusterin in colorectal carcinoma in relation to clinicopathological criteria[J].Malays J Pathol,2017,39(3):243-250.
[13] Gregory JM,Whiten DR,Brown RA,et al.Clusterin protects neurons against intracellular proteotoxicity[J].ActaNeuropathol Commun,2017,5(1):81.
[14] Tsiolaki PL,Nastou KC,Louros NN,et al.Exploring amyloidogenicity of clusterin:a structural and bioinformatics analysis[J].Adv Exp Med Biol,2017,989:93-107.
[15] Jin R,Chen X,Han D,et al.Clusterin modulates transdifferentiation of non-small-cell lung cancer[J].BMC Cancer,2017,17(1):661.
[16]臧晔.细胞减灭术联合腹腔灌注热化疗对卵巢癌患者免疫功能及血浆clusterin、septin-9蛋白表达的影响[J].中国妇幼保健,2018,33(03):674-677.
[17] Zheng W,Yao M,Qian Q,et al.Oncogenic secretory clusterin in hepatocellular carcinoma:Expression at early staging and emerging molecular target[J].Oncotarget,2017,8(32):52321-52332.
[18] Mustafi S,Sant DW,Liu ZJ,et al.Ascorbate induces apoptosis in melanoma cells by suppressing Clusterin expression[J].Sci Rep,2017,7(1):3671.