夏枯草提取物对自发性高血压大鼠血压及血管活性物质的影响
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摘要
目的:观察夏枯草提取物对自发性高血压大鼠血压的控制情况及相关指标的变化。方法:对照组为10只WKY大鼠,并将筛选出的40只自发性高血压大鼠(SHR)随机分为:模型组、阳性药组、夏枯草提取物高剂量组、夏枯草提取物低剂量组,每组10只。从14周龄时起给药,1次/d,连续灌胃5周。每周测量大鼠体质量。同时收集大鼠收缩压和行为学指标,给药5周后取材。运用ELISA检测大鼠给药5周后血清ANGⅡ、NE、ET、CGRP、TXB2的水平。结果:与模型组相比,阳性药组、夏枯草提取物高剂量组、夏枯草提取物低剂量组对收缩压均有一定程度的降低(P<0.05),其中,夏枯草提取物高剂量组的降压效果优于夏枯草提取物低剂量组,阳性药组无显著变化(P>0.05);随给药时间的延长,旋转耐受时间、易激惹程度评分均有不同程度的改善(P<0.05),阳性药组无显著变化(P>0.05),进行夏枯草提取物干预5周后,与模型组相比,阳性药组、夏枯草提取物高剂量组血清ANGⅡ浓度低于模型组(P<0.05);各组血清NE、ET、TXB2浓度均低于模型组(P<0.05),CGRP浓度均高于模型组(P<0.05)。结论:夏枯草提取物降压的作用机理可能与降低血清中、NE、ET含量,增加血清中CGRP含量相关,且存在量效关系。
Objective:To observe the control of Prunella vulgaris L. extract on blood pressure and the changes of related indexes in spontaneously hypertensive rats. Methods: Ten WKY rats in the control group were randomly divided into 40 spontaneously hypertensive rats(SHR): model group, positive drug group, Prunella vulgaris extract high dose group, Prunella vulgaris extract low dose group, 10 in each group. The drug was administered from the age of 14 weeks, once a day, and continuously administered for 5 weeks. Rat body weight was measured weekly. At the same time, the systolic blood pressure and behavioral indexes of the rats were collected and taken out after 5 weeks administration. The levels of serum ANGII, NE, ET, CGRP and TXB2 were measured by ELISA for 5 weeks after administration. Results: Compared with the model group, the positive drug group, the high-dose and low-dose groups of Prunella vulgaris L. had a certain degree of reduction in systolic blood pressure(P<0.05), among which the high dose of Prunella vulgaris L. extract had better antihypertensive effect thanthe low dose group of Prunella vulgaris L. extract, and there was no significant change in the positive drug group(P>0.05). With the prolongation of the administration time, the rotation tolerance time and irritability scores were improved to different extents(P<0.05). There was no significant change in the positive drug group(P>0.05). After 5 weeks intervention with Prunella vulgaris L. extract, the serum ANGII concentration of the positive drug group and the high dose group of Prunella vulgaris L. extract was lower than that of the model group. The concentrations of NE, ET and TXB2 in each group were lower than that in the model group(P<0.05), and the concentration of CGRP was higher than that in the model group(P<0.05). Conclusion: The mechanism of antihypertensive effect of Prunella vulgaris L. extract may be related to reducing serum NE and ET contents and increasing serum CGRP content, and there is a dose-effect relationship.
Objective:To observe the control of Prunella vulgaris L. extract on blood pressure and the changes of related indexes in spontaneously hypertensive rats. Methods: Ten WKY rats in the control group were randomly divided into 40 spontaneously hypertensive rats(SHR): model group, positive drug group, Prunella vulgaris extract high dose group, Prunella vulgaris extract low dose group, 10 in each group. The drug was administered from the age of 14 weeks, once a day, and continuously administered for 5 weeks. Rat body weight was measured weekly. At the same time, the systolic blood pressure and behavioral indexes of the rats were collected and taken out after 5 weeks administration. The levels of serum ANGII, NE, ET, CGRP and TXB2 were measured by ELISA for 5 weeks after administration. Results: Compared with the model group, the positive drug group, the high-dose and low-dose groups of Prunella vulgaris L. had a certain degree of reduction in systolic blood pressure(P<0.05), among which the high dose of Prunella vulgaris L. extract had better antihypertensive effect thanthe low dose group of Prunella vulgaris L. extract, and there was no significant change in the positive drug group(P>0.05). With the prolongation of the administration time, the rotation tolerance time and irritability scores were improved to different extents(P<0.05). There was no significant change in the positive drug group(P>0.05). After 5 weeks intervention with Prunella vulgaris L. extract, the serum ANGII concentration of the positive drug group and the high dose group of Prunella vulgaris L. extract was lower than that of the model group. The concentrations of NE, ET and TXB2 in each group were lower than that in the model group(P<0.05), and the concentration of CGRP was higher than that in the model group(P<0.05). Conclusion: The mechanism of antihypertensive effect of Prunella vulgaris L. extract may be related to reducing serum NE and ET contents and increasing serum CGRP content, and there is a dose-effect relationship.
引文
[1] 国家卫生计生委合理用药专家委员会,中国医师协会高血压专业委员会.高血压合理用药指南[J].中国医学前沿杂志(电子版),2015,7(6):22-64.
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[6] 马雪玲,李玉波,陈建新,等.自发性高血压大鼠中医证候及其理化指标相关性研究[J].世界中医药,2013,8(2):134-137.
[7] 薛晓兴,李玉波,廉洪建,等.高血压肝火亢盛证动物模型相关指标的研究[J].中国实验方剂学杂志,2015,21(8):97-101.
[8] 梁健钦,熊万娜,罗远,等.夏枯草提取物对大鼠自发性高血压降血压作用研究[J].中药材,2011,34(1):99-100.
[9] 王磊,高兴州,姜厚望,等.夏枯草配伍钩藤对自发性高血压大鼠血压及心脏的影响[J].中医药导报,2017,23(19):19-22,33.
[10] SaNCHEZ D,KASSAN M,CONTRFRAS MDELM,et al.Long-term intake of a milk casein hydrolysate attenuates the develoPment of hyPertension and involves cardiovascular benets[J].Pharmacological Research,2011,63(5):398-404.
[11] 程治源,叶挺,凌秋洋,等.血管紧张素Ⅱ通过ERK1/2通路促进血管内皮细胞钙化[J].安徽医科大学学报,2018,53(4):542-546.
[12] 许爱梅,庄向华,董砚虎.内皮素-1的研究进展[J].国外医学(内科学分册),2004(5):198-200,230.
[13] 刘倍吟,魏程科,李应东.当归挥发油对高血压模型大鼠血压及血管炎症反应的影响[J].中国中医药信息杂志,2016,23(11):71-74.
[14] 陈华,陈芝芸,严茂祥,等.理气化痰祛瘀法对非酒精性脂肪性肝炎大鼠血浆前列环素及血栓素的影响[J].中华中医药学刊,2007,25(1):87-89.
[15] 方利,刘健,朱福兵,等.基于细胞因子/NF-κB信号通路探讨强直性脊柱炎患者血瘀状态形成的机制[J].中华中医药学刊,2016,34(12):2913-2917.
[16] 李金海,龚云,张洪彬,等.慢性阻塞性肺疾病并肺动脉高压患者内皮素-1、血栓素B2检测的临床意义[J].实用医药杂志,2016,33(9):802-803.
[17] 杨波,张道亮,周琍珍,等.保心合剂对慢性心力衰竭患者ET CGRP及心功能的影响[J].中华中医药学刊,2008,26(6):1232-1233.
[18] 谢文霞,杨建荣,陈勇,等.腹针对颈性眩晕患者血浆ET-1 CGRP水平影响的研究[J].中华中医药学刊,2010,28(4):871-873.
[19] DO JY,BACK SY,LlM SD,et al.Effects of casein hydrolysate on the systolic blood Pressure and serum liPid Profiles in sPontaneously hyPertensive rats[J].Korean Journal for Food Science of Animal Resources,2010,30(4):545-550.
[20] 英俊岐,种宝贵.降钙素基因相关肽与心血管病关系研究进展[J].中国心血管杂志,2001(2):118-120.
[21] 梅全喜.鼻咽癌的中医药治疗[M].北京:中国中医药出版社,2010:114.
[2] 刘播,丁秀婷.高血压药物治疗方式及常用药物研究进展[J].中国药业,2015(23):253-255.
[3] 彭晓飞,边育红.药物治疗高血压研究进展[J].慢性病学杂志,2013(8):609-611.
[4] RLDWOOD H.HyPertension socity and Public Policy[J].Eur Heart J,2007,9(suppl B):B13-B18
[5] 梁君昭,董秋芬,苏建,等.高血压病中医证素分布研究[J].河南中医,2018,38(2):241-243.
[6] 马雪玲,李玉波,陈建新,等.自发性高血压大鼠中医证候及其理化指标相关性研究[J].世界中医药,2013,8(2):134-137.
[7] 薛晓兴,李玉波,廉洪建,等.高血压肝火亢盛证动物模型相关指标的研究[J].中国实验方剂学杂志,2015,21(8):97-101.
[8] 梁健钦,熊万娜,罗远,等.夏枯草提取物对大鼠自发性高血压降血压作用研究[J].中药材,2011,34(1):99-100.
[9] 王磊,高兴州,姜厚望,等.夏枯草配伍钩藤对自发性高血压大鼠血压及心脏的影响[J].中医药导报,2017,23(19):19-22,33.
[10] SaNCHEZ D,KASSAN M,CONTRFRAS MDELM,et al.Long-term intake of a milk casein hydrolysate attenuates the develoPment of hyPertension and involves cardiovascular benets[J].Pharmacological Research,2011,63(5):398-404.
[11] 程治源,叶挺,凌秋洋,等.血管紧张素Ⅱ通过ERK1/2通路促进血管内皮细胞钙化[J].安徽医科大学学报,2018,53(4):542-546.
[12] 许爱梅,庄向华,董砚虎.内皮素-1的研究进展[J].国外医学(内科学分册),2004(5):198-200,230.
[13] 刘倍吟,魏程科,李应东.当归挥发油对高血压模型大鼠血压及血管炎症反应的影响[J].中国中医药信息杂志,2016,23(11):71-74.
[14] 陈华,陈芝芸,严茂祥,等.理气化痰祛瘀法对非酒精性脂肪性肝炎大鼠血浆前列环素及血栓素的影响[J].中华中医药学刊,2007,25(1):87-89.
[15] 方利,刘健,朱福兵,等.基于细胞因子/NF-κB信号通路探讨强直性脊柱炎患者血瘀状态形成的机制[J].中华中医药学刊,2016,34(12):2913-2917.
[16] 李金海,龚云,张洪彬,等.慢性阻塞性肺疾病并肺动脉高压患者内皮素-1、血栓素B2检测的临床意义[J].实用医药杂志,2016,33(9):802-803.
[17] 杨波,张道亮,周琍珍,等.保心合剂对慢性心力衰竭患者ET CGRP及心功能的影响[J].中华中医药学刊,2008,26(6):1232-1233.
[18] 谢文霞,杨建荣,陈勇,等.腹针对颈性眩晕患者血浆ET-1 CGRP水平影响的研究[J].中华中医药学刊,2010,28(4):871-873.
[19] DO JY,BACK SY,LlM SD,et al.Effects of casein hydrolysate on the systolic blood Pressure and serum liPid Profiles in sPontaneously hyPertensive rats[J].Korean Journal for Food Science of Animal Resources,2010,30(4):545-550.
[20] 英俊岐,种宝贵.降钙素基因相关肽与心血管病关系研究进展[J].中国心血管杂志,2001(2):118-120.
[21] 梅全喜.鼻咽癌的中医药治疗[M].北京:中国中医药出版社,2010:114.