葛根素对大鼠下颌骨骨髓源巨噬细胞破骨细胞向分化的影响

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英文篇名：Effects of puerarin on osteoclast differentiation of marrow-derived macrophages from mandible 作者：马旭辉 ; 张鹏 ; 杨屹羚 ; 徐弘远 ; 龚心怡 ; 周巳入 ; 江凌勇 ; 代庆刚 英文作者：MA Xu-hui;ZHANG Peng;YANG Yi-ling;XU Hong-yuan;GONG Xin-yi;ZHOU Si-ru;JIANG Ling-yong;DAI Qing-gang;Department of Oromaxillofacial Head and Neck Oncology, Shanghai Jiao Tong University School of Medicine;The 2nd Dental Centre, Shanghai Jiao Tong University School of Medicine;Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology; 关键词：葛根素 ; 骨质疏松 ; 下颌骨骨髓源巨噬细胞 ; 破骨细胞 英文关键词：Puerarin;;Osteoporosis;;Mandible bone marrow-derived macrophages;;Osteoclast 中文刊名：ZGKQ 英文刊名：China Journal of Oral and Maxillofacial Surgery 机构：上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科;上海交通大学医学院附属第九人民医院·口腔医学院口腔第二门诊部;上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科正颌-正畸中心国家口腔疾病临床医学研究中心上海市口腔医学重点实验室上海市口腔医学研究所; 出版日期：2019-05-15 出版单位：中国口腔颌面外科杂志 年：2019 期：v.17 基金：国家自然科学基金(81570950,81870740,81800949);; SMC-晨星青年学者奖励计划优秀青年教师(B类计划);; 上海高校高峰高原学科建设项目;; 上海交通大学医学院附属第九人民医院“精才培育项目”(JC201809) 语种：中文; 页：ZGKQ201903002 页数：5 CN：03 ISSN：11-4980/R 分类号：9-13

摘要

目的:分析不同浓度的葛根素对于下颌骨骨髓源巨噬细胞(Bone marrow-derived macrophages,BMMs)破骨分化的影响。方法:体外分离培养4周龄雌性大鼠下颌骨BMMs。通过CCK-8分析1 nmol/L、10 nmol/L、100 nmol/L、1μmol/L、10μmol/L和100μmol/L葛根素对BMMs细胞活性的影响。使用RANKL诱导BMMs破骨分化的同时加入不同浓度葛根素,采用抗酒石酸酸性磷酸酶(TRAP)酶化学染色及q-PCR分析破骨细胞形成及破骨相关基因的改变。采用SPSS16.0软件包对数据进行统计学分析。结果:1 nmol/L、10 nmol/L、100 nmol/L、1μmol/L、10μmol/L葛根素组与对照组活细胞数无显著差异,100μmol/L组活细胞数较对照组降低(P<0.05)。RANKL可诱导BMMs形成多核破骨细胞。10 nmol/L、100 nmol/L及1μmol/L葛根素组破骨细胞数目及破骨细胞面积均较对照组降低(P<0.05)。q-PCR分析发现,10 nmol/L、100 nmol/L及1μmol/L葛根素组NFATc1、C-fos、TRAP及CTSK mRNA表达量均较对照组下降(P<0.05)。结论:葛根素可抑制下颌骨BMMs破骨分化及相关基因表达,为葛根素应用于下颌骨骨质疏松症的预防与治疗提供了依据。
        PURPOSE: To study the effect of puerarin on osteoclast differentiation of marrow-derived macrophages(BMMs) from mandible. METHODS: BMMs were obtained from 4-week-old female SD rats. Cell viability of BMMs with different concentration of puerarin was determined by CCK-8. Tartrate resistant acid phosphatase staining and q-PCR were used to analyse the changes of osteoclast formation and expression of osteoclast gene of BMMs due to different concentration of puerarin. Statistical analysis was performed using SPSS 16.0 software package. RESULTS: Cell viability of BMMs with puerarin at 1 nmol/L, 10 nmol/L, 100 nmol/L, 1 μmol/L and 10 μmol/L showed no difference from the control group, and 100 μmol/L group displayed decreased cell viability(P<0.05). Osteoclast number and relative osteoclast surface reduced in BMMs with 10 nmol/L, 100 nmol/L and 1 μmol/L puerarin in comparison with the control group.Furthermore, the expression of NFATc1, C-fos, TRAP and CTSK deceased in BMMs with 10 nmol/L, 100 nmol/L,and 1 μmol/L puerarin. CONCLUSIONS: Puerarin could impede osteoclast differentiation of mandibular BMMs, which may be used to prevent and treat osteoporosis in alveolar and jaw bone.

引文

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