丁香总黄酮抗氧化活性研究
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摘要
以2,6-二叔丁基-4-甲基苯酚(BHT)对照品、槲皮素对照品和丁香(Eugenia caryophyllata Thunb.)挥发油为对照,对丁香总黄酮还原力、清除1,1-二苯基-2-三硝基苯肼(DPPH·自由基)能力进行了评价。结果表明,丁香总黄酮有较好的抗氧化活性,其还原力略低于阳性对照BHT而高于丁香挥发油,清除DPPH·自由基能力则随浓度增大明显增强,浓度达到0.15、0.20 mg/mL时,其DPPH·自由基清除率分别超过丁香挥发油和阳性对照BHT。
The antioxidant activity of total flavonoids from Caryophylli flos was studied with 2,6-tert butyl-4-methyl phenol(BHT), quercetin, and essential oil from Caryophylli flos as reference, and the total reducing capacity and scavenging DPPH· free radical ability as evaluating indicator. The total flavonoids of Eugenia Cargophyllata Thunb. had better antioxidant activity,and its reducing power was slightly lower than that of positive control BHT and higher than that of essential oil from Caryophylli flos. The DPPH· free radical scavenging capacity increased obviously with the increase of concentration. When the concentration reached 0.15 and 0.2 mg/mL,DPPH· radical scavenging rate exceeded essential oil from Caryophylli flos and positive control BHT respectively.
The antioxidant activity of total flavonoids from Caryophylli flos was studied with 2,6-tert butyl-4-methyl phenol(BHT), quercetin, and essential oil from Caryophylli flos as reference, and the total reducing capacity and scavenging DPPH· free radical ability as evaluating indicator. The total flavonoids of Eugenia Cargophyllata Thunb. had better antioxidant activity,and its reducing power was slightly lower than that of positive control BHT and higher than that of essential oil from Caryophylli flos. The DPPH· free radical scavenging capacity increased obviously with the increase of concentration. When the concentration reached 0.15 and 0.2 mg/mL,DPPH· radical scavenging rate exceeded essential oil from Caryophylli flos and positive control BHT respectively.
引文
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[2]王巨媛,翟胜.植物精油应用进展及开发前景展望[J].江苏农业科学,2010(4):1-3.
[3]于君,姜子涛,李荣.天然调味香料丁香精油的研究进展[J].中国调味品,2014,39(8):109-114.
[4]谭业华,陈珍.丁香在海南扩种栽培的气候适应性分析[J].江西农业学报,2007(10):113-114.
[5]朱金段,袁德俊,林新颖.丁香的药理研究现状及临床应用[J].中国药物经济学,2013(1):32-35.
[6]苏筱渲,徐琳,吴娜.丁香挥发油的体外抗氧化作用研究[J].河南科技,2011(5):66-67.
[7]曹纬国,刘志勤,邵云,等.黄酮类化合物药理作用的研究进展[J].西北植物学报,2003,23(12):2241-2247.
[8]李英嘉,刘洪章.丁香挥发油的研究进展[J].黑龙江农业科学,2014(7):148-151.
[9]王焕芸,王玉华,贾彦胜,等.丁香药材中槲皮素的定性鉴别和含量测定[J].内蒙古医学院学报,2008,30(S1):534-537.
[10]王胡格吉乐图,奥·乌力吉.蒙药七味广枣丸研究进展[J].内蒙古民族大学学报(自然科学版),2013,28(1):91-93.
[11]李雨玲.银木挥发性成分及总黄酮抗氧化的研究[D].武汉:湖北中医药大学,2014.