不同产地凌霄花中齐墩果酸和熊果酸含量比较研究
|
摘要
目的:建立HPLC法同时测定凌霄花中齐墩果酸和熊果酸,比较不同产地的凌霄花中两种成分的含量。方法:选用Shim-pack VP-ODS C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸为流动相进行梯度洗脱,流速为1.0 mL/min,检测波长为215 nm,柱温为30℃。结果:齐墩果酸和熊果酸进样量分别在0.233 2～5.830 0μg和0.274 8～6.870 0μg范围内呈线性,回归方程分别为Y=6.337×10~5X+1.538×10~3(r=0.999 6)和Y=8.158×10~5X+2.977×10~3(r=0.999 7),平均加样回收率依次为98.9%和98.7%。采自连云港、南京的凌霄花中齐墩果酸和熊果酸的含量略高于其他4个产地。结论:所建立的方法可以准确的对凌霄花中齐墩果酸和熊果酸进行含量测定,不同产地的凌霄花中两种成分的含量存在差异。
Objective: To establish the HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Chinese trumpet vine, and compare the content of two components in Chinese trumpet vine from different habitats.Methods: Selected Shim-pack VP-ODS C_(18)(250 mm × 4.6 mm, 5 μm) chromatographic column, and conducted a gradient elution with acetonitrile-0.1% phosphoric acid as mobile phase,with flow rate being 1.0 mL/min, detection wavelength 215 nm, and column temperature 30 ℃. Results: The values of oleanolic acid and ursolic acid were linear in the range of 0.233 2～5.830 0 μg and 0.274 8～6.870 0 μg,respectively. The regression equations were Y=6.337 × 10~5 X+ 1.538×10~3(r=0.999 6) and Y=8.158×10~5 X+2.977×10~3(r=0.999 7),and the average sample recovery was 98.9% and 98.7% respectively. The content of oleanolic acid and ursolic acid in Chinese trumpet vine from Lianyungang and Nanjing was slightly higher than that in the other four habitats. Conclusion: The established method can be used to determine the content of oleanolic acid and ursolic acid in Chinese trumpet vine,and there were differences in the content of two components in different habitats of Chinese trumpet vine.
Objective: To establish the HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Chinese trumpet vine, and compare the content of two components in Chinese trumpet vine from different habitats.Methods: Selected Shim-pack VP-ODS C_(18)(250 mm × 4.6 mm, 5 μm) chromatographic column, and conducted a gradient elution with acetonitrile-0.1% phosphoric acid as mobile phase,with flow rate being 1.0 mL/min, detection wavelength 215 nm, and column temperature 30 ℃. Results: The values of oleanolic acid and ursolic acid were linear in the range of 0.233 2～5.830 0 μg and 0.274 8～6.870 0 μg,respectively. The regression equations were Y=6.337 × 10~5 X+ 1.538×10~3(r=0.999 6) and Y=8.158×10~5 X+2.977×10~3(r=0.999 7),and the average sample recovery was 98.9% and 98.7% respectively. The content of oleanolic acid and ursolic acid in Chinese trumpet vine from Lianyungang and Nanjing was slightly higher than that in the other four habitats. Conclusion: The established method can be used to determine the content of oleanolic acid and ursolic acid in Chinese trumpet vine,and there were differences in the content of two components in different habitats of Chinese trumpet vine.
引文
[1]宋学华,高似奇.凌霄花的本草考证[J].江苏药学与临床研究,2003,11(2):30-32.
[2]国家药典委员会.中华人民共和国药典[M].一部.北京:中国医药科技出版社,2015:287.
[3]江灵礼,苗明三.凌霄花化学、药理及临床应用特点探讨[J].中医学报,2014,29(7):1016-1018.
[4]刘丽娟,马英丽,王秀坤.凌霄花中齐墩果酸的分离鉴定(简报)[J].中国中药杂志,1993,18(7):429.
[5]闫庆梓,柏玉冰,唐洁,等.夏枯草中齐墩果酸和熊果酸的含量测定方法研究[J].中南药学,2017,15(8):1028-1031.
[6]刘慧妍,黄馨慧,张艳海.HPLC法快速测定8种中药中齐墩果酸和熊果酸[J].中草药,2017,48(10):1998-2001.
[7]古力齐曼·阿布力孜,阿吾提·艾买尔,毛居代·亚尔买买提,等.HPLC法测定野蔷薇中齐墩果酸和熊果酸的含量[J].食品工业科技,2016(6):49-51,57.
[8]周巧霞,顾振纶.山楂中熊果酸和齐墩果酸的高效液相色谱分析[J].中成药,2010,32(5):862-864.
[9]张俊清,陈文芝,蒋德锡,等.RP-HPLC法测定角花胡颓子中齐墩果酸与熊果酸的含量[J].药物分析杂志,2010,30(7):1305-1307.
[2]国家药典委员会.中华人民共和国药典[M].一部.北京:中国医药科技出版社,2015:287.
[3]江灵礼,苗明三.凌霄花化学、药理及临床应用特点探讨[J].中医学报,2014,29(7):1016-1018.
[4]刘丽娟,马英丽,王秀坤.凌霄花中齐墩果酸的分离鉴定(简报)[J].中国中药杂志,1993,18(7):429.
[5]闫庆梓,柏玉冰,唐洁,等.夏枯草中齐墩果酸和熊果酸的含量测定方法研究[J].中南药学,2017,15(8):1028-1031.
[6]刘慧妍,黄馨慧,张艳海.HPLC法快速测定8种中药中齐墩果酸和熊果酸[J].中草药,2017,48(10):1998-2001.
[7]古力齐曼·阿布力孜,阿吾提·艾买尔,毛居代·亚尔买买提,等.HPLC法测定野蔷薇中齐墩果酸和熊果酸的含量[J].食品工业科技,2016(6):49-51,57.
[8]周巧霞,顾振纶.山楂中熊果酸和齐墩果酸的高效液相色谱分析[J].中成药,2010,32(5):862-864.
[9]张俊清,陈文芝,蒋德锡,等.RP-HPLC法测定角花胡颓子中齐墩果酸与熊果酸的含量[J].药物分析杂志,2010,30(7):1305-1307.