江西野生毛花猕猴桃群体SSR遗传多样性研究
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摘要
为分析江西野生毛花猕猴桃群体遗传多样性,以江西省境内的5个野生毛花猕猴桃雄性群体(72个个体)为试验材料,采用SSR分子标记技术,选取15对多态性引物,利用聚丙烯酰胺电泳对PCR产物进行检测。结果表明,15对SSR引物共检测到86个位点,多态性位点百分率为100. 0%;观察到的平均等位基因数为5. 733,有效等位基因数为3. 002,Shannon信息指数为1. 046。表观杂合度介于0. 111～0. 819之间,预期杂合度介于0. 041～0. 876之间,遗传分化系数为2. 01,野生毛花猕猴桃雄性群体间存在较大的遗传分化。5个毛花猕猴桃雄性群体遗传距离范围为0. 102～0. 409,遗传相似度范围为0. 665～0. 903,群体间遗传距离与地理距离无相关性。群体的遗传多样性丰富度依次为庐山>井冈山>南源山>武功山>麻姑山,江西地区供试的野生毛花猕猴桃雄性群体在分子水平上具有丰富的多态性。本研究结果为毛花猕猴桃雄性品种选育、种质创新与利用提供了一定的理论基础。
In order to analyse genetic divertsities of 72 individuals from five wild male populations of Actinidia eriantha in Jiangxi province of China,we selected 15 pairs of primers with high polymorphism and used polyacrylamide gel electrophoresis to detect PCR products based on SSR technique. A total of 86 polymorphic loci were identified using 15 pairs of primers,and 100% of the loci identified were polymorphic. The mean number of observed number of alleles,effect alleles per locus and Shannon' s information index was 5. 733,3. 002 and 1. 046,respectively. Observed heterozygosity,expected heterozygosity and the genetic differentiation coefficient was ranged about 0.042 ～ 0.819,0.041～ 0.876 and 2.01,respectively,which meant that there were large genetic differences among the wild male A. eriantha populations. The genetic distance and the genetic identity of the five populations were 0.102 ～ 0.409 and 0.665 ～ 0.903 respectively,and no relationship was observed between genetic distance and geographical distance. The relative richness of genetic diversity among the five wild male A. eriantha populations was as following: Lushanshan > Jingangshan >Nanyuanshan>Wugongshan>Magushan. The results of this study showed that a high level of genetic diversity was existed in male A. eriantha of Jiangxi province,and the results can also be useful for breeding new varieties of A. eriantha and germplasm innovation and utilization.
In order to analyse genetic divertsities of 72 individuals from five wild male populations of Actinidia eriantha in Jiangxi province of China,we selected 15 pairs of primers with high polymorphism and used polyacrylamide gel electrophoresis to detect PCR products based on SSR technique. A total of 86 polymorphic loci were identified using 15 pairs of primers,and 100% of the loci identified were polymorphic. The mean number of observed number of alleles,effect alleles per locus and Shannon' s information index was 5. 733,3. 002 and 1. 046,respectively. Observed heterozygosity,expected heterozygosity and the genetic differentiation coefficient was ranged about 0.042 ～ 0.819,0.041～ 0.876 and 2.01,respectively,which meant that there were large genetic differences among the wild male A. eriantha populations. The genetic distance and the genetic identity of the five populations were 0.102 ～ 0.409 and 0.665 ～ 0.903 respectively,and no relationship was observed between genetic distance and geographical distance. The relative richness of genetic diversity among the five wild male A. eriantha populations was as following: Lushanshan > Jingangshan >Nanyuanshan>Wugongshan>Magushan. The results of this study showed that a high level of genetic diversity was existed in male A. eriantha of Jiangxi province,and the results can also be useful for breeding new varieties of A. eriantha and germplasm innovation and utilization.
引文
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[15]翟金良.我国猕猴桃产业存在的问题及发展对策[J].科技促进发展,2015(4):521-529
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[18]陆敏佳,蒋玉蓉,陆国权,陈国林,毛前.利用SSR标记分析藜麦品种的遗传多样性[J].核农学报,2015,29(2):260-269
[19]赵雅楠,王颖,张东杰,王丽侠,佐兆杭.中国东北地区绿豆种质资源遗传多样性的SSR分析[J].核农学报,2017,31(12):2340-2349
[20]董研,张军,任亚超,韩志校.中国新疆野苹果天然群体遗传多样性SSR分析[J].植物遗传资源学报,2013,14(5):771-777
[21]杨军,曹玉芬,吴俊,田路明,董星光,高源.杜梨实生繁殖群体遗传多样性的SSR分析[J].西北植物学报,2011,31(11):2172-2177
[22]艾呈祥,秦志华,王洁,王长君,安淼.山东牛心柿群体遗传多样性和亲缘关系的SSR分析[J].果树学报,2013,30(4):558-562
[23]宛甜,蔡宇良,冯瑛,张雪,何恒流.野生毛樱桃SSR遗传多样性和遗传结构分析[J].西北植物学报,2013,33(8):1544-1550
[24]刘亚令.猕猴桃属植物自然居群的遗传结构与种间基因渐渗研究[D].武汉:华中农业大学,2006
[25]汤佳乐,吴寒,郎彬彬,曲雪艳,黄春辉,徐小彪.野生毛花猕猴桃叶片和果实As A含量的SSR标记关联分析[J].园艺学报,2014,41(5):833-840
[26]王斯妤,钟敏,黄春辉,陶俊杰,曲雪艳,吴寒,徐小彪.猕猴桃属33份核心雄性种质SSR指纹图谱构建[J].分子植物育种,2018,16(5):1598-1606
[27]钟敏,黄春辉,朱博,陶俊杰,曲雪艳,吴寒,徐小彪.毛花猕猴桃观赏授粉兼用型优株MG-15[J].中国果树,2017(2):71-101
[28]芦娟,柴春山,吴文俊,戚健莉.文冠果干燥叶片高质量DNA提取方法研究[J].湖南农业科学,2013(5):1-4
[29]孟蒙,唐维,刘嘉,黄胜雄,余进德,刘方方,刘永胜.基于中华猕猴桃“红阳”转录组序列开发EST-SSR分子标记[J].应用与环境生物学报,2014,20(4):564-570
[30]汤佳乐,黄春辉,吴寒,郎彬彬,曲雪艳,徐小彪.野生毛花猕猴桃果实表型性状及SSR遗传多样性分析[J].园艺学报,2014,41(6):1198-1206
[31]徐小彪.江西猕猴桃资源的分布与评价[C]//中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集.上海:中国园艺学会,2008:232-233
[32]郝蕾,张磊,张国盛,王颖,韩胜利,白玉荣.北沙柳群体遗传多样性和遗传结构分析[J].西北植物学报,2017,37(8):1507-1516
[33]胡菀,罗意,阳亿,张志勇,范邓妹.野生桂花的遗传多样性和遗传结构研究[J].园艺学报,2014,41(7):1427-1435
[34] Zhe W,Ming K,Liu H,Jiao G,Zhang Z D,Li Y Y,P X M. High-level Genetic diversity and complex population structure of siberianapricot(Prunus sibirica L.)in China as revealed by nuclear SSRmarkers[J]. PLoS One,2014,9(2):873-881
[35] Frankham R,Ballou J D,Briscoe D A. Introduction to ConservationGenetics[M]. Cambrige:Cambrige University Press,2002:309-336
[36]刘亚令,李作洲,姜正旺,刘义飞,黄宏文.中华猕猴桃和美味猕猴桃自然居群遗传结构及其种间杂交渐渗[J].植物生态学报,2008,16(3):704-718
[2]栗琪,李作洲,黄宏文.猕猴桃野生居群的SSR分析初报[J].武汉植物学研究,2004,22(2):175-178
[3] Seal,A G. Plant breeding challenges to making kiwifruit a worldwidemainstream fresh fruit[J]. Acta Horticulturae,2003,610:75-80
[4]余敷霖,王慧明.毛花猕猴桃-沙农18号[J].福建果树,1981(2):25-26
[5]谢鸣,吴延军,蒋桂华,张庆朝,张慧琴,彭尚进,刘康猛.大果毛花猕猴桃新品种‘华特’[J].园艺学报,2008,35(10):1555-1561
[6]徐小彪,黄春辉,曲雪艳,陈明,钟敏,郎彬彬,张文标.毛花猕猴桃新品种‘赣猕6号’[J].园艺学报,2015,42(12):2539-2540
[7]张慧琴,谢鸣,肖金平,周利秋,宋根华.毛花猕猴桃‘华特’果实发育特性研究[J].果树学报,2015,32(2):238-246
[8]曲雪艳,郎彬彬,钟敏,朱博,陶俊杰,黄春辉,徐小彪.野生毛花猕猴桃果实品质主成分分析及综合评价[J].中国农学通报,2016,32(1):92-96
[9]戚雯烨,周晨卉,宋丽君,钟雨,郑小林.毛花猕猴桃‘华特’果实采后糖代谢研究[J].果树学报,2016,33(6):744-751
[10]杨鲁琼.花粉直感对‘布鲁诺’、‘华特’猕猴桃果实品质影响的研究[D].金华:浙江师范大学,2015
[11]杨鲁琼,常路伟,张慧琴,谢鸣.猕猴桃花粉直感现象的研究进展[J].安徽农业科学,2015,43(9):26-27
[12]吴寒,张晓慧,朱博,曲雪艳,黄春辉,徐小彪.野生毛花猕猴桃雄株花粉活性比较[J].中国南方果树,2014,43(3):118-123
[13]王斯妤,钟敏,廖光联,陈璐,徐小彪.不同猕猴桃雄株花粉量及花粉活力差异研究[J].江西农业大学学报,2017,39(3):460-467
[14]钟敏,谢敏,张文标,陶俊杰,黄春辉,朱博,徐小彪.野生毛花猕猴桃雄株居群花粉形态观察[J].果树学报,2016,33(10):1251-1258
[15]翟金良.我国猕猴桃产业存在的问题及发展对策[J].科技促进发展,2015(4):521-529
[16]杨妙贤,潘伟明,周玲艳,刘文,叶婵娟,刘胜洪,梁红. 3个野生毛花猕猴桃居群的SSR多样性分析[J].华南师范大学学报(自然科学版),2014,46(4):87-92
[17] Powell W,Morgante M,Andre C,Hanafey M,Vogel J,Tingey,SL. The comparison of RFLP, RAPD, AFLP and SSR(microsatellite)markers for germplasm analysis[J]. MolecularBreeding,1996,2(3):225-238
[18]陆敏佳,蒋玉蓉,陆国权,陈国林,毛前.利用SSR标记分析藜麦品种的遗传多样性[J].核农学报,2015,29(2):260-269
[19]赵雅楠,王颖,张东杰,王丽侠,佐兆杭.中国东北地区绿豆种质资源遗传多样性的SSR分析[J].核农学报,2017,31(12):2340-2349
[20]董研,张军,任亚超,韩志校.中国新疆野苹果天然群体遗传多样性SSR分析[J].植物遗传资源学报,2013,14(5):771-777
[21]杨军,曹玉芬,吴俊,田路明,董星光,高源.杜梨实生繁殖群体遗传多样性的SSR分析[J].西北植物学报,2011,31(11):2172-2177
[22]艾呈祥,秦志华,王洁,王长君,安淼.山东牛心柿群体遗传多样性和亲缘关系的SSR分析[J].果树学报,2013,30(4):558-562
[23]宛甜,蔡宇良,冯瑛,张雪,何恒流.野生毛樱桃SSR遗传多样性和遗传结构分析[J].西北植物学报,2013,33(8):1544-1550
[24]刘亚令.猕猴桃属植物自然居群的遗传结构与种间基因渐渗研究[D].武汉:华中农业大学,2006
[25]汤佳乐,吴寒,郎彬彬,曲雪艳,黄春辉,徐小彪.野生毛花猕猴桃叶片和果实As A含量的SSR标记关联分析[J].园艺学报,2014,41(5):833-840
[26]王斯妤,钟敏,黄春辉,陶俊杰,曲雪艳,吴寒,徐小彪.猕猴桃属33份核心雄性种质SSR指纹图谱构建[J].分子植物育种,2018,16(5):1598-1606
[27]钟敏,黄春辉,朱博,陶俊杰,曲雪艳,吴寒,徐小彪.毛花猕猴桃观赏授粉兼用型优株MG-15[J].中国果树,2017(2):71-101
[28]芦娟,柴春山,吴文俊,戚健莉.文冠果干燥叶片高质量DNA提取方法研究[J].湖南农业科学,2013(5):1-4
[29]孟蒙,唐维,刘嘉,黄胜雄,余进德,刘方方,刘永胜.基于中华猕猴桃“红阳”转录组序列开发EST-SSR分子标记[J].应用与环境生物学报,2014,20(4):564-570
[30]汤佳乐,黄春辉,吴寒,郎彬彬,曲雪艳,徐小彪.野生毛花猕猴桃果实表型性状及SSR遗传多样性分析[J].园艺学报,2014,41(6):1198-1206
[31]徐小彪.江西猕猴桃资源的分布与评价[C]//中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集.上海:中国园艺学会,2008:232-233
[32]郝蕾,张磊,张国盛,王颖,韩胜利,白玉荣.北沙柳群体遗传多样性和遗传结构分析[J].西北植物学报,2017,37(8):1507-1516
[33]胡菀,罗意,阳亿,张志勇,范邓妹.野生桂花的遗传多样性和遗传结构研究[J].园艺学报,2014,41(7):1427-1435
[34] Zhe W,Ming K,Liu H,Jiao G,Zhang Z D,Li Y Y,P X M. High-level Genetic diversity and complex population structure of siberianapricot(Prunus sibirica L.)in China as revealed by nuclear SSRmarkers[J]. PLoS One,2014,9(2):873-881
[35] Frankham R,Ballou J D,Briscoe D A. Introduction to ConservationGenetics[M]. Cambrige:Cambrige University Press,2002:309-336
[36]刘亚令,李作洲,姜正旺,刘义飞,黄宏文.中华猕猴桃和美味猕猴桃自然居群遗传结构及其种间杂交渐渗[J].植物生态学报,2008,16(3):704-718