大亚湾夏季表层浮游细菌生物量分布与环境变量的关系
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摘要
根据2008年夏季(7月)在大亚湾进行的现场综合调查资料,分析表层海水浮游细菌生物量的水平分布特征,探讨细菌生物量与环境变量的关系。大亚湾夏季浮游细菌生物量变化范围为14.00～118.80μg·L~(-1),海区平均值为55.23±29.43μg·L~(-1)。大亚湾夏季细菌生物量水平分布大体上呈现南部高于北部的趋势,在北纬22°35′以北和以南的海区均各自表现为东南部高于西北部的趋势。水深、硝酸盐、铵盐、亚硝酸盐和总碱度等5个参数与浮游细菌生物量之间存在着中等水平的相关性(0.15 On the basis of the cruise in Daya Bay in summer(July) 2008, the distribution patterns of bacterioplankton biomass were studied in surface layer in the subtropical bay. The relationships between bacterial biomass and environmental variables were analyzed. The results showed that the bacterial biomass ranged from 14.00 to 118.80 μg·L~(-1); and the mean bacterial biomass was 55.23±29.43 μg·L~(-1). Spatially, the bacterial biomass of station surface layers in south of the bay was higher than that in the north of the bay. In each marine areas located in the south or north of north longitude 22°35′ in this bay, it was higher in southeast of the bay than in northwest of the bay. There existed moderate correlationships between five environmental parameters including depth, nitrate, ammonia, nitrite and total alkalinity(0.15
引文
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[19] 李旭光,齐占会,林琳,等.基于高通量测序分析的大鹏澳海域沉积物古菌群落结构初步研究[J].南方水产科学,2015,11(6):1-8.
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[29] 姜发军,胡章立,胡超群.大鹏湾浮游细菌时空分布与环境因子的关系[J].热带海洋学报,2011,30(1):96-100.
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[31] 王聪,林军,陈丕茂,等.大亚湾水交换的数值模拟研究[J].南方水产,2008,4(4):8-15.
[32] 唐森铭,严岩,陈彬.春夏季大亚湾核电厂温排水对海洋浮游植物群落结构的影响[J].应用海洋学学报,2013,32(3):373-382.
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[34] 牟德海,林展江,李建生,等.大亚湾核电站循环海水出水口水质及泡沫成分分析[C].中国核学会.全国放射性流出物和环境监测与评价研讨会论文汇编[G],2003:173-178.
[35] RIVKIN RB,ANDERSON MR.Inorganic nutrient limitation of oceanic bacterioplankton[J].Limonol Oceanogr,1997,42(4):730-740.
[36] JOINT I,HENRIKSEN P,FONNES GA,et al.Competition for inorganic nutrients between phytoplankton and bacterioplankton in nutrient manipulated mesocosms[J].Aquat Microb Ecol,2002,29 (2):145-159.
[37] ZUBKOV MV,TARRAN GA.High bacterivory by the smallest phytoplankton in the North Atlantic Ocean[J].Nature, 2008,455(7210):224-226.
[38] 郑天凌,王海黎,洪华生.微生物在碳的海洋生物地球化学循环中的作用[J].生态学杂志,1994,13(4):47-50.
[39] JENNIFER C,JAMES AAE,ELLEN TM.Utilization and turnover of labile dissolved organic matter by bacterial heterotrophs in eastern North Pacific surface waters[J].Mar Ecol Prog Ser,1996,139 (1-3):267-279.
[40] CHO B C,AZAM F.Major role of bacteria in biogeochemical fluxes in the ocean's interior[J].Nature,1988,332(6163):441-443.
[41] KIRCHMAN DL,KEIL RG,SIMON M.Biomass and production of heterotrophic bacterioplankton in the oceanic subarctic Pacific[J].Deep-Sea Res,1993,40(5):967-988.
[42] SHIAH F,DUKLOW HW.Temperature regulation of heterotrophic bacterioplankton abundance,production,and specific growth rate in Chesapeake Bay[J].Limnol Oceanogr,1994,39(6):1243-1258.
[43] POMEROY LR,WIEBE WJ.Energy sources for microbial food webs[J].Mar Microb Food Webs,1993,7(1):101-118.
[44] NAGANUMA T,MIURA S.Abundance,production and viability of bacterioplankton in the Seto Inland Sea,Japan [J].J Oceanogr,1997,53(5):435-442.
[45] COLE J J,FINDLAY S,PACE M L.Bacterial production in fresh and saltwater ecosystems:a cross-system overview[J].Mar Ecol Prog Ser,1998,43 (1-2):1 -10.
[46] NAGANUMA T.Abundance and production of bacterioplankton along a transect of Ise Bay,Japan[J].J Oceanogr,1997,53(6):579 -583.
[47] 赵三军,肖天,岳海东.秋季东、黄海异养细菌(Heterotrophic Bacteria)的分布特点[J].海洋与湖沼,2003,34(3):295-305
[48] 周伟华,王汉奎,董俊德,等.海南岛三亚湾浮游植物和细菌生物量时空分布特征及环境制约因素研究[J].海洋学报,2007,29(6):110-118.
[49] CHO B C,CHOI J K,CHUNG C S,et al.Uncoupling of bacteria and phytoplankton during a spring diatom bloom in the mouth of the Yellow Sea[J].Mar Ecol Prog Ser,1994,115(1-2):181-190.
[2] SONG X,HUANG L,ZHANG J,et al.Variation of phytoplankton biomass and primary production in Daya Bay during spring and summer[J].Mar Pollut Bull,2004,49(11-12):1036-1044.
[3] WANG Y,LOU Z,SUN C,et al.Ecological environment changes in Daya Bay,China,from 1982 to 2004[J].Mar Pollut Bullet,2008,56(11):1871-1879.
[4] AZAM F,FENCHEL T,FIELD JG,et al.The ecological role of water column microbes in the sea[J].Mar Ecol Prog Ser,1983,10(3):257-263.
[5] DELONG EF,KARL DM.Genomic perspectives in microbial oceanography[J].Nature,2005,437(7057):336-342.
[6] PAERL HW,DYBLE J,TWOMEY L,et al.Characterizing man-made and natural modifications of microbial diversity and activity in coastal ecosystems[J].Antonie van Leeuwenhoek,2002,81 (1):487-507.
[7] 彭安国,黄奕普,刘广山,等.大亚湾细菌生产力研究[J].海洋学报,2003,25(4):83-90.
[8] 吴建平,蔡创华,周毅频,等.大亚湾网箱养殖水体弧菌种类组成及变化[J].湛江海洋大学学报,2006,26(4):42-48.
[9] 古小莉,李纯厚.大亚湾海洋异养细菌的初步研究[J].南方水产,2009,5(4):64-68.
[10] 江晓,任春华,胡超群,等.分子鉴定方法研究大亚湾水体弧菌种类变化[J].热带海洋学报,2010,29 (4): 154-159.
[11] 江晓亮,王朝晖,王剑,等.大亚湾海域可培养细菌鉴定及系统发育分析[J].生态科学,2013,32 (3):290-297.
[12] 王朝晖,乔倩,江晓亮,等.大亚湾浮游细菌的变性梯度凝胶电泳分析[J].环境科学学报,2016,36(4):1212-1218.
[13] 严瑶仙,梁柱源,朱泉剑,等.大亚湾南澳海域浮游细菌丰度对营养盐添加的响应[J].浙江万里学院学报,2014,27(1):1-9.
[14] 徐恭昭,等.大亚湾环境资源[M].合肥:安徽科学技术出版社,1989:144-161
[15] 吴建平,蔡创华,周毅频,等.大亚湾网箱养殖区异养细菌和弧菌的数量动态[J].湛江海洋大学学报,2006,26(3):21-25.
[16] 朱志红,林钦,黄洪辉.大鹏澳网箱养殖区春季细菌的时空分布特征[J].海洋环境科学,2011,30(1):68-71.
[17] FRY J.Bacterial diversity and unculturables[J].Microbiol Today,2000,27(1):186-188.
[18] 姚蜜蜜,周进,李庆赟,等.浮游细菌群落变化对锥状斯氏藻藻华的影响[J].现代生物医学进展,2015,15(2):201-207.
[19] 李旭光,齐占会,林琳,等.基于高通量测序分析的大鹏澳海域沉积物古菌群落结构初步研究[J].南方水产科学,2015,11(6):1-8.
[20] BOETIUS A,LOCHTE K.Effect of organic enrichments on hydrolytic potentials and growth of bacteria in deep-sea sediments[J].Mar Ecol Prog Ser,1996,140(1-3):239-250.
[21] 郑天凌,王斐,徐美珠,等.台湾海峡海域细菌产量、生物量及其在微食物环中的作用[J].海洋与湖沼,2002,33 (4):415-423.
[22] SEO E-Y,AHN T-S,ZO Y-G.Agreement,precision and accuracy of epifluorescence microscopy methods for enumeration of total bacterial number[J].Appl Environ Microbiol,2010,76(6):1981-1991.
[23] LEE S,FUHRMAN JA.Relationships between biovolume and biomass of naturally derived marine bacterioplankton[J].Appl Environ Microbiol,1987,53(6):1298-1303.
[24] KREMPIN DW,SULLIVAN CW.The seasonal abundance,vertical distribution,and relative microbial biomass of chroococcoid cyanobacteria at a station in southern California coastal waters[J].Can J Microbiol,1981,27(12):1341-1344.
[25] HARRIS GP.Picoplankton ecology:structure,function and fluctuations[M].London:Chapman and Hall Press,1986:221-235.
[26] 黄自强,张克,许昆灿,等.中华人民共和国海水水质标准(GB 3097-1997)[S],北京:中国环境科学出版社,1997.
[27] 周凯,章洁香,张瑜斌,等.深圳湾浮游细菌生物量的时空分布及其影响因素[J].热带海洋学报,2013,32(3):65-71.
[28] 林燕顺,曾活水,倪纯治,等.大亚湾水体中微生物量的分布特征[C]//国家海洋局第三海洋研究所.大亚湾海洋生态文集(Ⅱ).北京:海洋出版社,1990:261-265.
[29] 姜发军,胡章立,胡超群.大鹏湾浮游细菌时空分布与环境因子的关系[J].热带海洋学报,2011,30(1):96-100.
[30] WU M,WANG Y,DONG J,et al.Investigation of spatial and temporal trends in water quality in Daya Bay,South China Sea[J].Inter J Env Res Pub Heal,2011,8(6):2352-2365.
[31] 王聪,林军,陈丕茂,等.大亚湾水交换的数值模拟研究[J].南方水产,2008,4(4):8-15.
[32] 唐森铭,严岩,陈彬.春夏季大亚湾核电厂温排水对海洋浮游植物群落结构的影响[J].应用海洋学学报,2013,32(3):373-382.
[33] 周巧菊.大亚湾热污染研究[D].上海:华东师范大学硕士学位论文,2007:7-8.
[34] 牟德海,林展江,李建生,等.大亚湾核电站循环海水出水口水质及泡沫成分分析[C].中国核学会.全国放射性流出物和环境监测与评价研讨会论文汇编[G],2003:173-178.
[35] RIVKIN RB,ANDERSON MR.Inorganic nutrient limitation of oceanic bacterioplankton[J].Limonol Oceanogr,1997,42(4):730-740.
[36] JOINT I,HENRIKSEN P,FONNES GA,et al.Competition for inorganic nutrients between phytoplankton and bacterioplankton in nutrient manipulated mesocosms[J].Aquat Microb Ecol,2002,29 (2):145-159.
[37] ZUBKOV MV,TARRAN GA.High bacterivory by the smallest phytoplankton in the North Atlantic Ocean[J].Nature, 2008,455(7210):224-226.
[38] 郑天凌,王海黎,洪华生.微生物在碳的海洋生物地球化学循环中的作用[J].生态学杂志,1994,13(4):47-50.
[39] JENNIFER C,JAMES AAE,ELLEN TM.Utilization and turnover of labile dissolved organic matter by bacterial heterotrophs in eastern North Pacific surface waters[J].Mar Ecol Prog Ser,1996,139 (1-3):267-279.
[40] CHO B C,AZAM F.Major role of bacteria in biogeochemical fluxes in the ocean's interior[J].Nature,1988,332(6163):441-443.
[41] KIRCHMAN DL,KEIL RG,SIMON M.Biomass and production of heterotrophic bacterioplankton in the oceanic subarctic Pacific[J].Deep-Sea Res,1993,40(5):967-988.
[42] SHIAH F,DUKLOW HW.Temperature regulation of heterotrophic bacterioplankton abundance,production,and specific growth rate in Chesapeake Bay[J].Limnol Oceanogr,1994,39(6):1243-1258.
[43] POMEROY LR,WIEBE WJ.Energy sources for microbial food webs[J].Mar Microb Food Webs,1993,7(1):101-118.
[44] NAGANUMA T,MIURA S.Abundance,production and viability of bacterioplankton in the Seto Inland Sea,Japan [J].J Oceanogr,1997,53(5):435-442.
[45] COLE J J,FINDLAY S,PACE M L.Bacterial production in fresh and saltwater ecosystems:a cross-system overview[J].Mar Ecol Prog Ser,1998,43 (1-2):1 -10.
[46] NAGANUMA T.Abundance and production of bacterioplankton along a transect of Ise Bay,Japan[J].J Oceanogr,1997,53(6):579 -583.
[47] 赵三军,肖天,岳海东.秋季东、黄海异养细菌(Heterotrophic Bacteria)的分布特点[J].海洋与湖沼,2003,34(3):295-305
[48] 周伟华,王汉奎,董俊德,等.海南岛三亚湾浮游植物和细菌生物量时空分布特征及环境制约因素研究[J].海洋学报,2007,29(6):110-118.
[49] CHO B C,CHOI J K,CHUNG C S,et al.Uncoupling of bacteria and phytoplankton during a spring diatom bloom in the mouth of the Yellow Sea[J].Mar Ecol Prog Ser,1994,115(1-2):181-190.