EDTA抗凝管对布鲁杆菌病虎红平板凝集试验造成假阳性结果的分析
|
摘要
目的研究EDTA(乙二胺四乙酸)抗凝管对布鲁杆菌病虎红平板凝集试验结果的影响。方法选取本溪地区牛羊养殖户40例,分别用促凝管和EDTA抗凝管各采集全血1管,进行布鲁杆菌病虎红平板凝集试验(筛查试验)和试管凝集试验(确诊试验),比较2种采血管的布鲁杆菌病检测结果。结果 40例中1例为布鲁杆菌病患者,其余均为布鲁杆菌阴性结果。虎红平板凝集试验结果显示,促凝管组阳性1例(2.5%),EDTA抗凝管组阳性40例(100.0%),两者结果差异有统计学意义(P<0.05);试管凝集试验结果显示,促凝管组阳性1例(2.5%),EDTA抗凝管组阳性1例(2.5%),两者结果差异无统计学意义(P>0.05)。结论 EDTA抗凝管对布鲁杆菌病虎红平板凝集试验结果有显著影响,普遍造成假阳性,而对试管凝集试验结果无显著影响。
Objective To study the effect of Edta anticoagulation blood collection vessel on the test results of Rose-Bengal Plate agglutination in Brucellosis. Methods A total of 40 cases were selected from cattle and sheep households in Benxi area, 1 tube of blood was sampled respectively by coagulation-collecting vessel and Edta anticoagulation blood collection vessel, and the test results of Brucelosis were compared between the two blood sampling tubes by Rose-Bengal Plate agglutination test(screening test) and serum tube agglutination test(confirmed test). Results Brucellosis was observed in 1 of 40 cases, and negative results were observed in the other 39 cases. The results of the Rose-Bengal Plate agglutination test showed 1 positive case(2.5%) was observed in coagulation-collecting vessels group and 40 positive cases(100%) were observed in the Edta anticoagulation blood collection vessel group, with the difference of statistical significance(P<0.05); The results of serum tube agglutination test showed that 1 positive case(2.5%) was observed in coagulation-collecting vessels group and 1 positive case(2.5%) was observed in the Edta anticoagulation blood collection vessel group, with the difference not statistically significant(P>0.05). Conclusion Edta anticoagulation blood collection vessel has a significant effect on the results of the Rose-Bengal Plate agglutination test, which generally results in false positive, but has no significant effect on the test results of serum tube agglutination.
Objective To study the effect of Edta anticoagulation blood collection vessel on the test results of Rose-Bengal Plate agglutination in Brucellosis. Methods A total of 40 cases were selected from cattle and sheep households in Benxi area, 1 tube of blood was sampled respectively by coagulation-collecting vessel and Edta anticoagulation blood collection vessel, and the test results of Brucelosis were compared between the two blood sampling tubes by Rose-Bengal Plate agglutination test(screening test) and serum tube agglutination test(confirmed test). Results Brucellosis was observed in 1 of 40 cases, and negative results were observed in the other 39 cases. The results of the Rose-Bengal Plate agglutination test showed 1 positive case(2.5%) was observed in coagulation-collecting vessels group and 40 positive cases(100%) were observed in the Edta anticoagulation blood collection vessel group, with the difference of statistical significance(P<0.05); The results of serum tube agglutination test showed that 1 positive case(2.5%) was observed in coagulation-collecting vessels group and 1 positive case(2.5%) was observed in the Edta anticoagulation blood collection vessel group, with the difference not statistically significant(P>0.05). Conclusion Edta anticoagulation blood collection vessel has a significant effect on the results of the Rose-Bengal Plate agglutination test, which generally results in false positive, but has no significant effect on the test results of serum tube agglutination.
引文
[1] 卫生部疾病预防控制局. 布鲁氏菌病防治手册[M]. 北京: 人民卫生出版社, 2008: 3.
[2] 中华人民共和国卫生部. WS 269—2007 布鲁氏菌病诊断标准 [S]. 北京: 人民卫生出版社, 2008.
[3] 柯春荣, 李文娟, 马顺高, 等. 一起通过性传播的布病疫情调查[J]. 疾病监测与控制, 2015, 9(3): 170-171.
[4] Carrera AI, Lopez RMJ, Sapina AM, et al. Probable transmission of brucellosis by breast milk[J]. J Trop Pediatr, 2006, 52: 380-381.
[5] Palanduz A, Palanduz S, Gruel K, et al. Brucellosis in a mother and her young infant: probable transmission of breast milk[J]. International J Infect Dis, 2000, 4: 55-56.
[6] 葛丽敏, 王光旭. 食源性布鲁氏菌病[J]. 中国地方病杂志, 2007, 22(3): 227-228.
[7] 吴俊彪. 非疫区布鲁菌病12例误诊分析[J]. 中国冶金工业医学杂志, 2015, 32(6): 724-725.
[8] 李宏旭, 李克玲, 魏学娟, 等. 布鲁氏菌病误诊基本情况及原因初探[J]. 宁夏医学杂志, 2014, 36(2): 180-181.
[9] 李玲, 王勤, 曹培义. 常规试管凝集试验与虎红平板试验判断布氏杆菌病的比较[J]. 中国卫生检验杂志, 2013, 23(7): 1749-1750.
[10] 胡永勤, 莫国华, 唐爱奇. 虎红平板凝集实验在布鲁菌病哨点医院监测中的作用与效果[J].中国卫生检验杂志, 2016, 26(21): 143-144.
[11] 石庆芬, 刘学真, 谭磊. 试管凝集试验与虎红平板凝集试验在布鲁氏杆菌病诊断中应用价值比较[J]. 中外医疗, 2017, 2(3): 105-106.
[12] 苏良, 欧新华, 张如胜, 等. 虎红平板凝集试验检测人血浆布氏菌抗体干扰来源分析及处理对策[J]. 现代预防医学, 2014, 41(24): 4486-4488.
[13] 吕迎辉. 新形势下布鲁氏菌病的预防和控制[J]. 中国卫生产业, 2015, 35(12): 97-99.
[14] 唐志坚. 布鲁氏菌病的流行病学调查及分析[J]. 中国卫生产业, 2015, 35(3): 7-8.
[15] 薛会珍, 刘爱平, 李海平. 用两种血清学方法检测布鲁氏菌病结果比较[J]. 医学动物防制, 2016, 32(4): 465-466.
[2] 中华人民共和国卫生部. WS 269—2007 布鲁氏菌病诊断标准 [S]. 北京: 人民卫生出版社, 2008.
[3] 柯春荣, 李文娟, 马顺高, 等. 一起通过性传播的布病疫情调查[J]. 疾病监测与控制, 2015, 9(3): 170-171.
[4] Carrera AI, Lopez RMJ, Sapina AM, et al. Probable transmission of brucellosis by breast milk[J]. J Trop Pediatr, 2006, 52: 380-381.
[5] Palanduz A, Palanduz S, Gruel K, et al. Brucellosis in a mother and her young infant: probable transmission of breast milk[J]. International J Infect Dis, 2000, 4: 55-56.
[6] 葛丽敏, 王光旭. 食源性布鲁氏菌病[J]. 中国地方病杂志, 2007, 22(3): 227-228.
[7] 吴俊彪. 非疫区布鲁菌病12例误诊分析[J]. 中国冶金工业医学杂志, 2015, 32(6): 724-725.
[8] 李宏旭, 李克玲, 魏学娟, 等. 布鲁氏菌病误诊基本情况及原因初探[J]. 宁夏医学杂志, 2014, 36(2): 180-181.
[9] 李玲, 王勤, 曹培义. 常规试管凝集试验与虎红平板试验判断布氏杆菌病的比较[J]. 中国卫生检验杂志, 2013, 23(7): 1749-1750.
[10] 胡永勤, 莫国华, 唐爱奇. 虎红平板凝集实验在布鲁菌病哨点医院监测中的作用与效果[J].中国卫生检验杂志, 2016, 26(21): 143-144.
[11] 石庆芬, 刘学真, 谭磊. 试管凝集试验与虎红平板凝集试验在布鲁氏杆菌病诊断中应用价值比较[J]. 中外医疗, 2017, 2(3): 105-106.
[12] 苏良, 欧新华, 张如胜, 等. 虎红平板凝集试验检测人血浆布氏菌抗体干扰来源分析及处理对策[J]. 现代预防医学, 2014, 41(24): 4486-4488.
[13] 吕迎辉. 新形势下布鲁氏菌病的预防和控制[J]. 中国卫生产业, 2015, 35(12): 97-99.
[14] 唐志坚. 布鲁氏菌病的流行病学调查及分析[J]. 中国卫生产业, 2015, 35(3): 7-8.
[15] 薛会珍, 刘爱平, 李海平. 用两种血清学方法检测布鲁氏菌病结果比较[J]. 医学动物防制, 2016, 32(4): 465-466.