运输应激对山羊血清和空肠中HSP27、HSP70和HSP90含量的影响
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摘要
为探讨运输应激对山羊血清和空肠中HSP27、HSP70和HSP90 3种热休克蛋白含量的影响,试验选取12只赣西山羊,随机分为对照组、运输应激2 h组和运输应激6 h组,应用双抗体夹心ELISA法测定山羊血清和空肠中3种热休克蛋白浓度。结果表明:与对照组相比,运输2 h山羊血清中HSP70和HSP90极显著升高(P<0.01),空肠中HSP70和HSP90显著升高(P<0.05),血清和空肠中HSP27有升高趋势(P>0.05);与对照组相比,运输6 h后血清中HSP70和HSP90分别极显著和显著升高(P<0.05和P<0.01),血清中HSP27含量以及空肠中3种蛋白含量均无显著变化(P>0.05);与运输2 h相比,运输6 h后3种蛋白含量都极显著降低(P<0.01)。结果显示,山羊对运输应激在细胞水平的适应性调节与血清中HSP70和HSP90蛋白水平升高有密切联系,运输2 h山羊通过上调HSP70和HSP90对应激导致的空肠损伤发挥细胞保护作用。
In order to explore the effects of transport stress on the content of three heat shock proteins(HSP27,HSP70 and HSP90)in goat serum and jejunum,12 goats from west jiangxi were randomly divided into the control group,2 h transport stress group and 6 h transport stress group,two antibody sandwich ELISA method was used to determine the concentration of three heat shock proteins in goat serum and jejunum.Results showed that HSP70 and HSP90 in serum were significantly increased in 2 h transport goat compared with control group,HSP70 and HSP90 in the jejunum were increased in 2 h transport goat,HSP27 in jejunum and serum was increased but no significant difference was found.HSP70 and HSP90 in serum were significantly increased after 6 h transport,but HSP27 was no difference compared with control group,there were no significant changes in three heat shock proteins in the jejunum after 6 h transport compared with control group,three heat shock proteins content were significantly reduced after 6 h transport compared with 2 h transport.The results showed that the adaptive regulation of goat's transport stress on the cell level was closely related to the increase of HSP70 and HSP90 protein levels in serum,and goat had a cellular protective effect on the 2 h transport stress which induced jejunal injury by upregulating HSP70and HSP90.
In order to explore the effects of transport stress on the content of three heat shock proteins(HSP27,HSP70 and HSP90)in goat serum and jejunum,12 goats from west jiangxi were randomly divided into the control group,2 h transport stress group and 6 h transport stress group,two antibody sandwich ELISA method was used to determine the concentration of three heat shock proteins in goat serum and jejunum.Results showed that HSP70 and HSP90 in serum were significantly increased in 2 h transport goat compared with control group,HSP70 and HSP90 in the jejunum were increased in 2 h transport goat,HSP27 in jejunum and serum was increased but no significant difference was found.HSP70 and HSP90 in serum were significantly increased after 6 h transport,but HSP27 was no difference compared with control group,there were no significant changes in three heat shock proteins in the jejunum after 6 h transport compared with control group,three heat shock proteins content were significantly reduced after 6 h transport compared with 2 h transport.The results showed that the adaptive regulation of goat's transport stress on the cell level was closely related to the increase of HSP70 and HSP90 protein levels in serum,and goat had a cellular protective effect on the 2 h transport stress which induced jejunal injury by upregulating HSP70and HSP90.
引文
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[22]Arnal M E,Lallès J P.Gut epithelial inducible heat-shock proteins and their modulation by diet and the microbiota[J].Nutr Rev,2016,74(3):181.
[23]Zhong X,Wang T,Zhang X,et al.Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets[J].Cell Stress Chaperon,2010,15(3):335-342.
[24]Hao Y,Gu X H,Wang X L.Overexpression of heat shock protei n 70 and its relat ionsh ip to i ntest i ne u nder acute heat stress in broilers:1.Intestinal structure and digestive function[J].Poultry Sci,2012,91(4):781.
[25]Zhong X,Zhang X H,Li X M,et al.Intestinal growth and morphology is associated with the increase in heat shock protein70 expression in weaning piglets through supplementation with glutamine[J].J Anim Sci,2011,89(11):3634.
[2]Xing T,Xu X L,Zhou G H,et al.The effect of transportation of broilers during summer on the expression of heat shock protein 70,postmortem metabolism and meat quality[J].JAnim Sci,2015,93(1):62-70.
[3]Schwartzkopf-Genswein K S,Faucitano L,Dadgar S,et al.Road transport of cattle,swine and poultry in North America and its impact on animal welfare,carcass and meat quality:Areview[J].Meat Sci,2012,92(3):227-243.
[4]Wan C,Yin P,Xu X,et al.Effect of simulated transpor t stress on the rat small intestine:A morphological and gene expression study[J].Res Vet Sci,2014,96(2):355-364.
[5]Ph i l l ips C J C,Sa nt u r t u n E.T he wel fa re of l ive st o ck transported by ship[J].Vet J,2013,196(3):309-314.
[6]Buckham Sporer K R,Weber P S,Burton J L,et al.Transportation of young beef bulls alters circulating physiological parameters that may be effective biomarkers of stress[J].JAnim Sci,2008,86(6):1325-1334.
[7]Zhong R Z,Liu H W,Zhou D W,et al.The effects of road transportation on physiological responses and meat quality in sheep differing in age[J].J Anim Sci,2011,89(11):3742-3751.
[8]李玉保,鲍恩东,王志亮,等.荧光定量RT-PCR法检测运输应激猪热休克蛋白mRNA转录水平[J].中国农业科学,2006,39(1):187-192.
[9]王雍,杨伟月,马晓晓,等.运输应激对肉牛血清中多种应激指标的影响[J].黑龙江畜牧兽医,2018(7):1-3.
[10]刘延鑫,王义翠,孙宇,等.运输应激牛不同组织HSPs mRNA和蛋白表达研究[J].黑龙江畜牧兽医,2018(13):75-78.
[11]Kannan G,Kouakou B,Terrill T H,et al.Endocrine,blood metabolite,and meat quality changes in goats as inf luenced by short-term,preslaughter stress[J].J Anim Sci,2003,81(6):1499-1507.
[12]Alcalde M J,Suárez M D,Rodero E,et al.Effects of farm management practices and transport duration on stress response and meat quality traits of suckling goat kids[J].Animal,2017,11(9):1626-1635.
[13]Soh n S H,Subraman i V K,Moon Y S,et al.Telomer ic DNA quantity,DNA damage,and heat shock protein gene expression as physiological stress markers in chickens[J].Poultry Sci,2012,91(4):829.
[14]Zhang M,Xin L,Bao E,et al.Variation in the expression of Hsp27,αB-crystallin mRNA and protein in heart and liver of pigs exposed to different transport times[J].Res Vet Sci 2011,90(3):432-438.
[15]鲍恩东,Sultan K R.运输应激猪肝脏中热应激蛋白的定位与表达[J].中国农业科学,2002,35(9):1130-1133.
[16]Deb R,Sajjanar B,Singh U,et al.Promoter variants at AP2box region of Hsp70.1 affect thermal stress response and milk production traits in Frieswal cross bred cattle[J].Gene,2013,532(2):230-235.
[17]Collier R J,Collier J L,Rhoads R P,et al.Invited Review:Genes Involved in the Bovine Heat Stress Response[J].JDairy Sci,2008,91(2):445-454.
[18]Khan I,Lee K L,Xu L,et al.Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition[J].Reproduction,2017,153(4):421-431.
[19]Nakamoto H,Fujita K,Ohtaki A,et al.Physical interaction between bacterial heat shock protein(Hsp)90 and Hsp70 chaperones mediates their cooperative action to refold denatured proteins[J].JBiol Chem,2014,289(9):6110-6119.
[20]Nabuurs M J,Van Essen G J,Nabuurs P,et al.Thirty minutes transport causes small intestinal acidosis in pigs[J].Res Vet Sci,2001,70(2):123-127.
[21]Rokutan K.Role of heat shock proteins in gastric mucosal protection[J].J Gastroen Hepatol,2000,15(Supplement s1):12-19.
[22]Arnal M E,Lallès J P.Gut epithelial inducible heat-shock proteins and their modulation by diet and the microbiota[J].Nutr Rev,2016,74(3):181.
[23]Zhong X,Wang T,Zhang X,et al.Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets[J].Cell Stress Chaperon,2010,15(3):335-342.
[24]Hao Y,Gu X H,Wang X L.Overexpression of heat shock protei n 70 and its relat ionsh ip to i ntest i ne u nder acute heat stress in broilers:1.Intestinal structure and digestive function[J].Poultry Sci,2012,91(4):781.
[25]Zhong X,Zhang X H,Li X M,et al.Intestinal growth and morphology is associated with the increase in heat shock protein70 expression in weaning piglets through supplementation with glutamine[J].J Anim Sci,2011,89(11):3634.
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