恩诺沙星对卵形鲳鲹消化酶、免疫酶及抗氧化酶活力的影响
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摘要
为探究采用恩诺沙星药浴方式对卵形鲳鲹生理酶活的影响,设置2个浸泡质量浓度处理组(5、10 mg/L),并以0 mg/L质量浓度组为对照组,在浸泡处理24 h后,对卵形鲳鲹肠、胃、肝、肾的消化酶、免疫酶、抗氧化酶等的活性进行检测。结果显示:采用低质量浓度(5 mg/L)恩诺沙星药浴可提高卵形鲳鲹肾组织中碱性磷酸酶(AKP)的活力,但高质量浓度(10 mg/L)则对AKP的活力有抑制作用,5 mg/L处理组[(5.01±2.06) U/g prot]显著高于10 mg/L处理组[(0.40±0.39) U/g prot](P<0.05);肾组织中超氧化物歧化酶(SOD)的活力由对照组的(1.45±0.31) U/mg prot显著上升至10 mg/L处理组的(4.64±1.69) U/mg prot(P<0.05);肾组织中丙二醛(MDA)的活力由对照组的(0.53±0.15) nmol/mg prot显著上升至10 mg/L处理组的(3.70±1.21) nmol/mg prot(P<0.05);谷胱甘肽过氧化物酶(GST-Px)的活力在肾组织中对照组和5 mg/L处理组均显著低于10 mg/L处理组(P<0.05)。研究表明,采用恩诺沙星溶液药浴,质量浓度在10 mg/L以下时不会对卵形鲳鲹造成不可逆的生理损伤,是一种较为安全高效的病害防治方法。
引文
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[22]沈洪艳,王冰,赵月,等.氧氟沙星对锦鲤抗氧化系统和DNA损伤的影响[J].环境科学与技术,2015,38(12):59-66.
[23]张喆,李健,冯伟,等.不同浓度诺氟沙星对中国对虾非特异性免疫酶活的影响[J].渔业科学进展,2011,32(2):53-59.
[24]王荻,李绍戊,马涛,等.诺氟沙星对两种鲟体内SOD活力影响的比较研究[J].中国畜牧兽医,2011,38(7):34-37.
[25]李绍戊,王荻,马涛,等.恩诺沙星对小体鲟和史氏鲟体内SOD活力影响的比较研究[J].水产学杂志,2011,24(2):37-40.
[26]卢彤岩,陈琛,李绍戊,等.恩诺沙星对2种鲟细胞色素P450活性影响的比较研究[J].中国农学通报,2011,27(20):87-91.
[27]DENG J M,MAI K S,AI Q H,et al.Alternative protein sources in diets for Japanese flounder Paralichthys olivaceus(Temminck and Schlegel):II. Effects on nutrient digestibility and digestive enzyme activity[J].Aquaculture Research,2010,41(6):861-870.
[28]谢一荣,吴锐全.鱼类消化酶研究及其在水产养殖中的应用[J].广东饲料,2005,14(02):15-18.
[29]张丽敏,吴灶和,简纪常,等.恩诺沙星对红笛鲷血清中碱性磷酸酶活力、抗体IgM含量、溶菌酶含量及其抗菌能力的影响[J].水产养殖,2009,30(4):1-7.
[30]应翔宇,杨永胜.兽用新型抗菌药物——恩诺沙星[J].中国兽药杂志,1995,29(3):53-56.
[2]杨火盛.卵形鲳鲹人工养殖试验[J].福建水产,2006(1):39-41.
[3]MA Z H,HU J,LIU Y J,et al.Insulin-like Growth Factor (IGF) genes in golden pompano Trachinotus ovatus(Linnaeus 1758) larvae: molecular cloning and response to water temperature and nutrient manipulation[J].The Israeli Journal of Aquaculture-Bamidgeh,2016,68:1334-1341.
[4]GUO S,MO Z,WANG Z,et al.Isolation and pathogenicity of Streptococcus iniae in offshore cage-cultured Trachinotus ovatus in China[J].Aquaculture,2018,492:247-252.
[5]但学明,李安兴,林小涛,等.卵形鲳鲹对刺激隐核虫的免疫应答和免疫保护研究[J].水生生物学报,2008,32(1):13-18.
[6]DUGENCI S K,ARDA N,CANDAN A.Some medicinal plants as immunostimulant for fish[J].Journal of Ethnopharmacology,2003,88(1):99-106.
[7]AGNEW W,BARNES A C.Streptococcus iniae:An aquatic pathogen of global veterinary significance and a challenging candidate for reliable vaccination[J].Veterinary Microbiology,2007,122(1):1-15.
[8]LYU K,ZHU X,WANG Q,et al.Copper/zinc superoxide dismutase from the Cladoceran Daphnia magna:molecular cloning and expression in response to different acute environmental stressors.[J].Environmental Science & Technology,2013,47(15):8887-8893.
[9]GE J,LI J,ZHANG J,et al.Time-dependent oxidative stress responses of submerged macrophyte Vallisneria natans seedlings exposed to ammonia in combination with microcystin under laboratory conditions[J].Bulletin of Environmental Contamination & To-xicology,2012,89(1):67-72.
[10]LI M,YU N,QIN J G,et al.Effects of ammonia stress,dietary linseed oil and Edwardsiella ictaluri challenge on juvenile darkbarbel catfish Pelteobagrus vachelli[J].Fish & Shellfish Immunology,2014,38(1):158.
[11]BITTERLICH G.Digestive enzyme pattern of two stomachless filter feeders,silver carp,Hypophthalmichthys molitrix Val.,and bighead carp,Aristichthys nobilis Rich[J].Journal of Fish Biology,1985,27(2):103-112.
[12]方星星.恩诺沙星在异育银鲫体内的药代动力学及免疫调节作用[Z].南京农业大学,2013.
[13]FANG X,LIU X,LIU W,et al.Pharmacokinetics of enrofloxacin in allogynogenetic silver crucian carp,Carassius auratus gibelio[J].Journal of Veterinary Pharmacology & Therapeutics,2012,35(4):397.
[14]LIU Y,HU J,ZHOU S,et al.Effect of acute ammonia stress on antioxidant enzymes and digestive enzymes in barramundi Lates calcarifer Larvae[J].The Israeli Journal of Aquaculture Bamidgeh,2018,15(8):1-11.
[15]HEGAZI M M,ATTIA Z I,ASHOUR O A.Oxidative stress and antioxidant enzymes in liver and white muscle of Nile tilapia juveniles in chronic ammonia exposure[J].Aquatic Toxicology,2010,99(2):118-125.
[16]丁美丽,林林,李光友,等.有机污染对中国对虾体内外环境影响的研究[J].海洋与湖沼,1997,28(1):7-12.
[17]NICHOLS T L,WHITEHOUSE C A,AUSTIN F E.Transcriptional analysis of a superoxide dismutase gene of Borrelia burgdorferi[J].Fems Microbiology Letters,2000,183(1):37.
[18]FAROMBI E O,ADELOWO O A,AJIMOKO Y R.Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African cat fish(Clarias gariepinus) from Nigeria Ogun River[J].International Journal of Environmental Research and Public Health,2007,4(2):158-165.
[19]朱钦士.我们的机体是如何解毒的[J].生物学通报,2013,48(7):13-19.
[20]杨帆,孙翰昌.恩诺沙星对鲫鱼过氧化氢酶活性的影响[J].湖北农业科学,2010(1):156-158.
[21]朱建勇,陈德碧.恩诺沙星对鲫鱼碱性磷酸酶活性的影响[J].安徽农业科学,2008,36(14):5904-5905.
[22]沈洪艳,王冰,赵月,等.氧氟沙星对锦鲤抗氧化系统和DNA损伤的影响[J].环境科学与技术,2015,38(12):59-66.
[23]张喆,李健,冯伟,等.不同浓度诺氟沙星对中国对虾非特异性免疫酶活的影响[J].渔业科学进展,2011,32(2):53-59.
[24]王荻,李绍戊,马涛,等.诺氟沙星对两种鲟体内SOD活力影响的比较研究[J].中国畜牧兽医,2011,38(7):34-37.
[25]李绍戊,王荻,马涛,等.恩诺沙星对小体鲟和史氏鲟体内SOD活力影响的比较研究[J].水产学杂志,2011,24(2):37-40.
[26]卢彤岩,陈琛,李绍戊,等.恩诺沙星对2种鲟细胞色素P450活性影响的比较研究[J].中国农学通报,2011,27(20):87-91.
[27]DENG J M,MAI K S,AI Q H,et al.Alternative protein sources in diets for Japanese flounder Paralichthys olivaceus(Temminck and Schlegel):II. Effects on nutrient digestibility and digestive enzyme activity[J].Aquaculture Research,2010,41(6):861-870.
[28]谢一荣,吴锐全.鱼类消化酶研究及其在水产养殖中的应用[J].广东饲料,2005,14(02):15-18.
[29]张丽敏,吴灶和,简纪常,等.恩诺沙星对红笛鲷血清中碱性磷酸酶活力、抗体IgM含量、溶菌酶含量及其抗菌能力的影响[J].水产养殖,2009,30(4):1-7.
[30]应翔宇,杨永胜.兽用新型抗菌药物——恩诺沙星[J].中国兽药杂志,1995,29(3):53-56.