米氏凯伦藻和东海原甲藻生长的光照强度生态幅研究

详细信息    查看全文 | 推荐本文 |

英文篇名：ECOLOGICAL AMPLITUDE FOR LIGHT INTENSITY OF KARENIA MIKIMOTOI AND PROROCENTRUM DONGHAIENSE AT DIFFERENT TEMPERATURES IN LABORATORY CULTURES 作者：文世勇 ; 林雨霏 ; 王紫竹 ; 孙田力 ; 李飞 ; 石晓勇 ; 刘永健 ; 赵建华 英文作者：WEN Shi-Yong;LIN Yu-Fei;WANG Zi-Zhu;SUN Tian-Li;LI Fei;SHI Xiao-Yong;LIU Yong-Jian;ZHAO Jian-Hua;National Marine Environmental Monitoring Center;Key Laboratory for Ecological Environment in Coastal Areas,State Oceanic Administration;National marine hazard mitigation service; 关键词：米氏凯伦藻 ; 东海原甲藻 ; 生态幅 ; 最适光强 ; 光照强度适宜生长范围 ; 光照强度耐受限度 英文关键词：Karenia mikimotoi;;Prorocentrum donghaiense;;ecological amplitude;;the optimum light intensity for algal growth;;suitable light intensity ranges for algal growth;;tolerance light intensity ranges for algal growth 中文刊名：HYFZ 英文刊名：Oceanologia et Limnologia Sinica 机构：国家海洋环境监测中心;国家海洋局近岸海域生态环境重点实验室;自然资源部海洋减灾中心; 出版日期：2019-05-15 出版单位：海洋与湖沼 年：2019 期：v.50 基金：国家重点研发计划“海洋环境安全保障”重点专项,2017YFC1404304号;; 国家自然科学基金项目,41371494号;; 浙江省海洋与海岛管理资金项目;; 浙江省海洋灾害防灾减灾资金项目 语种：中文; 页：HYFZ201903020 页数：8 CN：03 ISSN：37-1149/P 分类号：196-203

摘要

藻类生长的光照生态辐是指在一定光照强度范围内藻类能生长和繁殖的水平范围,由藻类生长的最适光照强度、光照强度适宜生长范围和光照强度耐受限度构成。为了定量获取藻类生长的光照生态幅,在室内培养条件下,分别研究了三个温度(18、22、25°C)条件下六个不同光照强度[28.32、55.15、75.06、96.59、111.66和135.75μmol/(m~2·s)]对米氏凯伦藻和东海原甲藻细胞数和最大比生长率的影响,依据Shelford耐受性定律建立了米氏凯伦藻和东海原甲藻的光照耐受性模型,并得到了藻类生长的最适光强、光强适宜生长范围和光强耐受限度的定量表达。结果表明:无论是米氏凯伦藻还是东海原甲藻,在同一温度条件下,在实验设定的光照强度水平范围内,均分别存在一个适宜藻类生长的最适光强Iopt,且当光强I≤Iopt时,藻类细胞密度和比生长率均随着光强的升高而显著增大;而当I≥Iopt时,藻类细胞密度和比生长率随着光强的升高而显著减小。此外,随着培养温度的升高,藻类细胞密度和比生长率均呈现"先升后降"的变化趋势。建立的藻类生长光照耐受性模型与Shelford耐受定律较为吻合,并定量得到了米氏凯伦藻在18、22、25°C下的最适生长光强分别为81.48、80.15、79.27μmol/(m~2·s);光强适宜生长范围分别为33.11—162.96、32.57—160.3、32.03—158.54μmol/(m~2·s);东海原甲藻在18、22、25°C下的最适生长光强分别为79.39、78.19、76.69μmol/(m~2·s);光强适宜生长范围分别为31.89—158.78、31.77—156.38、31.18—153.38μmol/(m~2·s)。
        The ecological amplitude for light intensity of algal growth refers to the level of growth and reproduction of algae within a certain light intensity range, which usually consists of the optimum, suitable, and tolerance light intensity ranges for algal growth. To obtain the ecological amplitude for light intensity of Karenia mikimotoi and Prorocentrum donghaiense, laboratory culture experiments comprising six levels of light intensity gradients [28.32, 55.15, 75.06, 96.59,111.66, and 135.75μmol/(m~2·s)] were carried out. The effects of these six levels on algal cell densities and specific growth rate of K. mikimotoi and P. donghaiense at three temperature gradients(18, 22, and 25°C) were determined. Moreover, a tolerance model between light intensity and specific growth rate of algae was established based on the experimental results and Shelford's law of tolerance. Finally, the optimum, suitable, and tolerance light intensity ranges for algal growth were obtained through the tolerance model. Results showed(1) an optimum light intensity for algal growth(Iopt) in the given light intensity gradient level under the same temperature conditions, regardless of K. mikimotoi or P. donghaiense. When I≤ Iopt, the algal cell densities and specific growth rate of K. mikimotoi and P. donghaiense increased significantly as light intensity increased(ANOVA, P<0.05); when I ≥ Iopt, the algal cell densities and specific growth rate decreased significantly as light intensity increased(ANOVA, P<0.01).(2) With the increase in temperature, algal cell densities and the specific growth rate of K. mikimotoi and P. donghaiense initially increased and decreased thereafter.(3) The light intensity tolerance model was consistent with Shelford's law of tolerance.(4) For K. mikimotoi cultures grown at the three temperatures(18, 22, and 25°C), the optimum light intensity values were 81.48, 80.15, and 79.27μmol/(m~2·s), respectively,and the suitable light intensity ranges were 33.11—162.96, 32.57—160.3, and 32.03—158.54μmol/(m~2·s), respectively.(5)For P. donghaiense cultures grown at the three temperatures(18, 22, and 25°C), the optimum light intensity values were79.39, 78.19 and 76.69μmol/(m~2·s), respectively, and the suitable light intensity ranges were 31.89—158.78,31.77—156.38 and 31.18—153.38μmol/(m~2·s), respectively.

引文

王爱军,王修林,王江涛等,2006.光照对东海赤潮高发区春季硅藻生长的影响.中国海洋大学学报,36(S1):173-178,162
    王爱军,王修林,韩秀荣等,2008.光照对东海赤潮高发区春季赤潮藻种生长和演替的影响.海洋环境科学,27(2):144-148
    邓华健,陶建华,2004.浮游植物增长光响应动力学实验研究.河北建筑科技学院学报,21(1):28-30,34
    孙百晔,2008.长江口及邻近海域浮游植物生长的光照效应研究[D].青岛:中国海洋大学博士学位论文
    孙百晔,王修林,李雁宾等,2008a.光照在东海近海东海原甲藻赤潮发生中的作用.环境科学,29(2):362-367
    孙百晔,梁生康,王长友等,2008b.光照与东海近海中肋骨条藻(Skeletonema costatum)赤潮发生季节的关系.环境科学,29(7):1849-1854
    杨东方,高振会,王培刚等,2002.光照时间和水温对浮游植物生长影响的初步剖析--以胶州湾为例.海洋科学,26(12):18-22
    沈国英,施并章,2002.海洋生态学.2版.北京:科学出版社
    陈艳拢,赵冬至,杨建洪等,2009.赤潮藻类温度生态幅的定量表达模型研究.海洋学报,31(5):156-161
    黄邦钦,洪华生,1994.几种藻类吸收磷酸盐动力学的初步研究.厦门大学学报(自然科学版),33(S1):7-11
    黄邦钦,洪华生,戴民汉,1993.环境因子对海洋浮游植物吸收磷酸盐速率的影响.海洋学报,15(4):64-67
    颜天,周名江,钱培元,2002.环境因子对塔玛亚历山大藻生长的综合影响.海洋学报,24(2):114-120
    Andersson A,Hajdu S,Haecky P et al,1996.Succession and growth limitation of phytoplankton in the Gulf of Bothnia(Baltic Sea).Marine Biology,126(4):791-801
    Baly E C C,1935.The kinetics of photosynthesis.Proceedings of the Royal Society B:Biological Sciences,117(804):218-239
    Blackman F F,1905.Optima and limiting factors.Annals of Botany,19(2):281-296
    Cloern J E,1978.Empirical model of Skeletonema costatum photosynthetic rate,with applications in the San Francisco bay estuary.Advances in Water Resources,1(5):267-274
    Dubinsky Z,Falkowski P G,Wyman K,1986.Light harvesting and utilization by phytoplankton.Plant and Cell Physiology,27(7):1335-1349
    Eppley R W,1972.Temperature and phytoplankton growth in the sea.Fishery Bulletin,70(4):1063-1085
    Geider R J,MacIntyre H L,Kana T M,1996.A dynamic model of photoadaptation in phytoplankton.Limnology and Oceanography,41(1):1-15
    Jassby A D,Platt T,1976.Mathematical formulation of the relationship between photosynthesis and light for phytoplankton.Limnology and Oceanography,21(4):540-547
    Kim D I,Matsuyama Y,Nagasoe S et al,2004.Effects of temperature,salinity and irradiance on the growth of the harmful red tide dinoflagellate Cochlodinium polykrikoides Margalef(Dinophyceae).Journal of Plankton Research,26(1):61-66
    Litchman E,Klausmeier C A,Bossard P,2004.Phytoplankton nutrient competition under dynamic light regimes.Limnology and Oceanography,49(4):1457-1462
    Longhi M L,Schloss I R,Wiencke C,2005.Effect of irradiance and temperature on photosynthesis and growth of two Antarctic benthic diatoms,Gyrosigma subsalinum and Odontella litigiosa.Botanica Marina,46(3):276-284
    Moisan J R,Moisan T A,Abbott M R,2002.Modelling the effect of temperature on the maximum growth rates of phytoplankton populations.Ecological Modelling,153(3):197-215
    Morton S L,Norris D R,Bomber J W,1992.Effect of temperature,salinity and light intensity on the growth and seasonality of toxic dinoflagellates associated with ciguatera.Journal of Experimental Marine Biology and Ecology,157(1):79-90
    Nalewajko C,Garside C,1983.Methodological problems in the simultaneous assessment of photosynthesis and nutrient uptake in phytoplankton as functions of light intensity and cell size.Limnology and Oceanography,28(3):591-597
    Raven J A,Geider R J,1988.Temperature and algal growth.New Phytologist,110(4):441-461
    Shelford V E,1911.Physiological animal geography.Journal of Morphology,22(3):551-618
    Shelford V E,1913.Animal Communities in Temperate America.Chicago,Illinois:University of Chicago Press
    Smith E L,1936.Photosynthesis in relation to light and carbon dioxide.Proceedings of the National Academy of Sciences of the United States of America,22(8):504-511
    Steele J H,1962.Environmental control of photosynthesis in the sea.Limnology and Oceanography,7(2):137-150
    Wynne D,Rhee G Y,1986.Effects of light intensity and quality on the relative N and P requirement(the optimum N:P ratio)of marine planktonic algae.Journal of Plankton Research,8(1):91-103
    Yamaguchi M,Honjo T,1989.Effects of temperature,salinity and irradiance on the growth of the noxious red tide flagellate Gymnodinium nagasakiense(Dinophyceae).Nippon Suisan Gakkaishi,55(11):2029-2036(in Japanese)
    Yamaguchi M,Shigeru I,Nagasaki K et al,1997.Effects of temperature and salinity on the growth of the red tide flagellates Heterocapsa circularisquama(Dinophyceae)and Chattonella verruculosa(Raphidophyceae).Journal of Plankton Research,19(8):1167-1174