溢油对小新月菱形藻氨基酸相对含量变化的影响
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摘要
以小新月菱形藻(Nitzschia closterium)为受试生物,在96 h急性毒性实验条件下,设置不同浓度的180#燃料油分散液(WAF),分析小新月菱形藻细胞内氨基酸相对含量的变化.结果表明,随着培养时间增加,对照组(Ctrl)和低浓度WAF(1%和3%)中丙氨酸和组氨酸相对含量呈上升趋势,而在高浓度WAF(5%、7%和10%)组中丙氨酸无明显变化趋势,组氨酸则呈先降低后升高的趋势.在对照组和低浓度WAF组(1%和3%)下,半胱氨酸相对含量随培养时间增加而减少,而高浓度WAF(5%、7%和10%)下,变化趋势与低浓度相反.在各WAF浓度下,脯氨酸相对含量均随着培养时间的增加而增加,而天冬氨酸的变化正好与脯氨酸相反.因此,本研究发现小新月菱形藻内的主要氨基酸对180#燃料油分散液具有敏感的生化反应,可作为海洋污染检测的一种新途径.
Nitzschia closterium( N. closterium) was selected as the test organism to study the toxicity effects of 180 # fuel oil water accommodated fractions( WAF) during the exposure time of 96 h in the present study. The relative abundance of amino acids in the cells of N. closterium were measured.The results showed that the relative abundance of alanine( Ala) and histidine( His) increased with increasing culture time in the control group and low WAF concentrations( 1% and 3%). However,the change of Ala was not obvious in the high WAF concentrations( 5%,7% and 10%),and the abundance of His first decreased and then increased. The relative abundance of cysteine( Cys) in the control group and low WAF concentrations( 1% and 3%) decreased with the increase of culture time,while variation trend was opposite in the high WAF concentrations( 5%,7% and 10%).The relative abundance of proline( Pro) increased with increasing culture time in all the WAF concentrations,and the change of aspartic acid( Asp) was just opposite to the trend of Pro. The study shows that the main amino acids in N. closterium are sensitive to the 180 # fuel oil WAF,which can serve as the new approach to monitor the marine pollution caused by the oil spill.
Nitzschia closterium( N. closterium) was selected as the test organism to study the toxicity effects of 180 # fuel oil water accommodated fractions( WAF) during the exposure time of 96 h in the present study. The relative abundance of amino acids in the cells of N. closterium were measured.The results showed that the relative abundance of alanine( Ala) and histidine( His) increased with increasing culture time in the control group and low WAF concentrations( 1% and 3%). However,the change of Ala was not obvious in the high WAF concentrations( 5%,7% and 10%),and the abundance of His first decreased and then increased. The relative abundance of cysteine( Cys) in the control group and low WAF concentrations( 1% and 3%) decreased with the increase of culture time,while variation trend was opposite in the high WAF concentrations( 5%,7% and 10%).The relative abundance of proline( Pro) increased with increasing culture time in all the WAF concentrations,and the change of aspartic acid( Asp) was just opposite to the trend of Pro. The study shows that the main amino acids in N. closterium are sensitive to the 180 # fuel oil WAF,which can serve as the new approach to monitor the marine pollution caused by the oil spill.
引文
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[13]王爱英,许鹏,崔喜艳.微拟球藻蛋白质含量和氨基酸组成分析[J].山东师范大学学报(自然科学版),2014,29(1):137-140.WANG A Y,XU P,CUI X Y. Analysis of protein and amino acid composition of Nannochlorpsis Oculata[J]. Journal of Shandong NormalUniversity(Natural Science),2014,29(1):137-140(in Chinese).
[14] BROMKE M A. Amino acid biosynthesis pathways in diatoms[J]. Metabolites,2013,3(2):294-311.
[15] WULANDARI A P,MIYAZAKI J,KOBASHI N,et al. Characterization of bacterial homocitrate synthase involved in lysine biosynthesis[J]. Febs Letters,2002,522(1-3):35-40.
[16] JOSHI V,JOUNG J G,FEI Z,et al. Interdependence of threonine,methionine and isoleucine metabolism in plants:Accumulation andtranscriptional regulation under abiotic stress[J]. Amino Acids,2010,39(4):933-947.
[17] STEPANSKY A,LEUSTEK T. Histidine biosynthesis in plants[J]. Amino Acids,2006,30(2):127-142.
[2]高祥,石晓勇,韩秀荣,等.石油烃类污染物对中肋骨条藻和微型原甲藻的毒性效应研究[J].海洋环境科学,2017,36(6):918-923.GAO X,SHI X Y,HAN R X,et al. Toxicity of petroleum hydrocarbon pollutants on Skeletonema Costatum and Prorocentrum Minimum[J]. Marine Environmental Science,2017,36(6):918-923(in Chinese).
[3]袁萍,吕振波,周革非.石油烃胁迫下3种微藻的生长动力学研究[J].海洋科学,2014,38(10):46-51.YUAN P,LZ B,ZHOU G F. Growth kinetics of 3 species of microalgae treated with petroleum hydrocarbon[J]. Marine Sciences,2014,38(10):46-51(in Chinese).
[4]马帅,曲良,李晓红,等.四种溢油分散剂对青岛大扁藻和小新月菱形藻细胞密度和叶绿素含量的影响[J].生态科学,2015,34(6):9-15.MA S,QU L,LI X H,et al. Effects of four dispersants on cell density and chlorophyll contents of Platymonas helgolandica var.tsingtaoensis and Nitzschia closterium f. minutissima[J]. Ecologlcal Science,2015,34(6):9-15(in Chinese).
[5]陈刚,肖慧,唐学玺. 3种海洋赤潮微藻蛋白质和核酸合成动态对芘胁迫的响应[J].海洋环境科学,2008,27(4):320-322.CHEN G,XIAO H,TANG Y X. Responses of three species of marine red-tide microalgae to pyrene Stress in protein and nucleic acidsynthesis[J]. Marine Environmental Science,2008,27(4):320-322(in Chinese).
[6]肖凯丽,张琳,邓百川,等.氨基酸对哺乳动物脂质代谢的调控及其作用机理[J].中国畜牧兽医,2017,44(12):3473-3481.XIAO K L,ZHANG L,DENG B C,et al. Regulation effects and mchanism of amino acids on lipid metabolism in mammals[J]. ChinaAnimal Husbandry and Veterinary Medicine,2017,44(12):3473-3481(in Chinese).
[7]曹淑莉,向葆卿. 8种海洋饵料微藻蛋白质含量及氨基酸组成比例的比较研究[J].海洋学报(中文版),1993,15(4):98-103.Cao S L,Xiang B Q. Comparative study on protein content and amino acid composition ratio of microalgae in eight marine foods[J].Haiyang Xuebao,1993,15(4):98-103(in Chinese).
[8] AKSMANN A,TUKAJ Z. Intact anthracene inhibits photosynthesis in algal cells:A fluorescence induction study on Chlamydomonasreinhardtii cw92 strain[J]. Chemosphere,2008,74(1):26-32.
[9] FAKSNESS L G,BRANDVIK P J,SYDNES L K. Composition of the water accommodated fractions as a function of exposure times andtemperatures[J]. Marine Pollution Bulletin,2008,56(10):1746-1754.
[10] MORALES-LOO M R,GOUTX M. Effects of water-soluble fraction of the mexican crude oil“Isthmus Cactus”on growth,cellular contentof chlorophyll a,and lipid composition of planktonic microalgae[J]. Marine Biology,1990,104(3):503-509.
[11]刘瑀,李颖,王晓琦,等.原油分散液对小新月菱形藻C、N稳定同位素组成的影响[J].海洋环境科学,2015,34(4):503-507.LIU Y,LI Y,WANG X Q,et al. Effect of crude oil dispersion on carbon and nitrogen stable isotopic composition of nitzschia closterium[J]. Marine Environmental Science,2015,34(4):503-507(in Chinese).
[12]王大志,朱友芳,李少菁,等.七种微藻蛋白质含量和氨基酸组成的比较[J].台湾海峡,1999,18(3):297-302.WANG D Z,ZHU Y F,LI S Q,et al. Studies on protein content and amino acid composition of seven microalgae[J]. Journal ofOceanography in Taiwan Strait,1999,18(3):297-302(in Chinese).
[13]王爱英,许鹏,崔喜艳.微拟球藻蛋白质含量和氨基酸组成分析[J].山东师范大学学报(自然科学版),2014,29(1):137-140.WANG A Y,XU P,CUI X Y. Analysis of protein and amino acid composition of Nannochlorpsis Oculata[J]. Journal of Shandong NormalUniversity(Natural Science),2014,29(1):137-140(in Chinese).
[14] BROMKE M A. Amino acid biosynthesis pathways in diatoms[J]. Metabolites,2013,3(2):294-311.
[15] WULANDARI A P,MIYAZAKI J,KOBASHI N,et al. Characterization of bacterial homocitrate synthase involved in lysine biosynthesis[J]. Febs Letters,2002,522(1-3):35-40.
[16] JOSHI V,JOUNG J G,FEI Z,et al. Interdependence of threonine,methionine and isoleucine metabolism in plants:Accumulation andtranscriptional regulation under abiotic stress[J]. Amino Acids,2010,39(4):933-947.
[17] STEPANSKY A,LEUSTEK T. Histidine biosynthesis in plants[J]. Amino Acids,2006,30(2):127-142.
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