基于气相色谱-飞行时间质谱联用技术的嗜麦芽窄食单胞菌的同源性分析
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摘要
目的探索脂肪酸分型方法对嗜麦芽窄食单胞菌同源性分析的可能性。方法收集南京市某医院住院患者痰液样本分离的嗜麦芽窄食单胞菌,经化学方法提取菌体脂肪酸后用气相色谱与飞行时间质谱联用仪(GC-TOF-MS)分析,获得该菌脂肪酸成分的数据资料,并利用SPSS 22.0软件进行聚类统计分析。结果 28株嗜麦芽窄食假单胞菌可根据脂肪酸组成及其含量分为4个型,A型18株,包括A_1亚型5株,A_2亚型13株,B型6株,包括B_1亚型4株,B_2亚型2株,C型3株,包括C_1亚型2株,C_2亚型1株,D型1株。主要脂肪酸为C14:0,C15:0,C16:0,C18:0,其中含量最高的脂肪酸成分是C16:0。结论基于GC-TOF-MS的嗜麦芽窄食假单胞菌脂肪酸分析具有操作简单、省时、自动化程度高等特点,可为细菌同源性分析提供有效的数据信息,为预防控制嗜麦芽窄食假单胞菌的医院内感染,实施有效的管理措施提供指导性意见。
Objective To explore the possibility of the homologous analysis of Stenotrophomonas maltophilia with the fatty acid(FA) typing method. Methods Stenotrophomonas maltophilia was isolated from the sputum samples in hospitalized patients from a Nanjing hospital. Fatty acids were extracted by chemical methods, and then analyzed by GC-TOF-MS so as to obtain the FA profiles data, and SPSS22.0 statistical software was used for cluster analysis. Results All the 28 strains were divided to 4 clones according to fatty acid composition and its contents. Clone A included 18 strains while A_1, A_2 subtype included five strains and thirteen strains, respectively. Clone B included 6 strains while B_1 included four strains and B_2 included two strains. Clone C included 3 strains while C_1, C_2 included two strains and one, respectively. Clone D included one strain. The main fatty acids were C14:0, C15:0, C16:0 and C18:0. The highest content of fatty acids was C16:0. Conclusion Fatty acid analysis of Stenotrophomonas maltophilia based on GC-TOF-MS method has the characteristics of easy operation, time saving and high degree of repetition rate and automation. It can provide effective information for bacterial homology analysis, guidance for preventing infection in hospital and implementing effective management measures.
Objective To explore the possibility of the homologous analysis of Stenotrophomonas maltophilia with the fatty acid(FA) typing method. Methods Stenotrophomonas maltophilia was isolated from the sputum samples in hospitalized patients from a Nanjing hospital. Fatty acids were extracted by chemical methods, and then analyzed by GC-TOF-MS so as to obtain the FA profiles data, and SPSS22.0 statistical software was used for cluster analysis. Results All the 28 strains were divided to 4 clones according to fatty acid composition and its contents. Clone A included 18 strains while A_1, A_2 subtype included five strains and thirteen strains, respectively. Clone B included 6 strains while B_1 included four strains and B_2 included two strains. Clone C included 3 strains while C_1, C_2 included two strains and one, respectively. Clone D included one strain. The main fatty acids were C14:0, C15:0, C16:0 and C18:0. The highest content of fatty acids was C16:0. Conclusion Fatty acid analysis of Stenotrophomonas maltophilia based on GC-TOF-MS method has the characteristics of easy operation, time saving and high degree of repetition rate and automation. It can provide effective information for bacterial homology analysis, guidance for preventing infection in hospital and implementing effective management measures.
引文
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[14] 吴大盈,朱延焱.367株嗜麦芽窄食单胞菌耐药性分析[J].药物流行病学杂志,2014,23(11):664-666.
[15] Falagas ME,Kastoris AC,Vouloumanou EK,et al.Attributable mortality of Stenotrophomonas maltophilia infections:a systematic review of the literature[J].Future Microbiol,2009,4(9):1103-1109.
[16] Huang YW,Hu RM,Lin YT,et al.The contribution of class 1 integron to antimicrobial resistance in Stenotrophomonas maltophilia[J].Microb Drug Resist,2015,21(1):90-96.
[17] Brooke JS.Stenotrophomonas maltophilia:an emerging global opportunistic pathogen[J].Clin Microbiol Rev,2012,25(1):2-41.
[18] 储从家,吴惠玲.445株嗜麦芽窄食单胞菌的分布及耐药性分析[J].中国微生态学杂志,2014,26(8):928-930.
[19] Zhang SK,Yin YL,Jones MB,et al.Salmonella serotype determination utilizing high-throughput genome sequencing data[J].J Clin Microbiol,2015,53(5):1685-1692.
[20] 陈经雕,邓小玲,柯昌文,等.广东省布鲁氏菌菌株脂肪酸成分分析[J].中国人兽共患病学报,2010,26(2):131-133.
[21] 狄慧玲,张耀祺,单潇潇,等.河北地区食源性单核细胞增生李斯特菌脂肪酸的分型研究[J].现代食品科技,2014,30(11):65-70.
[2] Amoli RI,Nowroozi J,Sabokbar A,et al.Isolation of Stenotrophomonas maltophilia,from clinical samples:an investigation of patterns motility and production of melanin pigment[J].Asian Pacific Journal of Tropical Biomedicine,2017(9):826-830.
[3] Xun M,Zhang Y,Li BL,et al.Clinical characteristics and risk factors of infections caused by Stenotrophomonas maltophilia in a hospital in northwest China[J].J Infect Dev Ctries,2014,8(8):1000-1005.
[4] Baranzelli A,Wallyn F,Nseir S.Infections bronchopulmonaires a Stenotrophomonas maltophilia et à Acinetobacter baumannii[J].Revue de Pneumologie Clinique,2013,69(5):250-259.
[5] George S,Karageorgopoulos DE,Sofia M,et al.Stenotrophomonas maltophilia Infections in a General Hospital:Patient Characteristics,Antimicrobial Susceptibility,and Treatment Outcome[J].PLoS ONE,2012,7(5):e37375.
[6] Phu VD,Wertheim HFL,Larsson M,et al.Burden of Hospital Acquired Infections and Antimicrobial Use in Vietnamese Adult Intensive Care Units[J].PLoS ONE,2016,11(1):e0147544.
[7] Abel K,Deschmertzing H,Peterson JI.Classification of microorganisms by analysis of chemical composition.I.feasibility of utilizing gas chromatography[J].J Bacteriol,1963,85(5):1039-1044.
[8] Okamura H,Tsutsui H,Komatsu T,et al.Cloning of a new cytokine that induces IFN-γ production by T cells[J].Nature,1995,378(6552):88.
[9] Gu Y,Kuida K,Tsutsui H,et al.Activation of interferon-gamma inducing factor mediated by interleukin-1beta converting enzyme[J].Science,1997,275(5297):206-209.
[10] William WC.Lipid analysis-isolation,separation,identification and structural analysis of lipids Ulberth F.Book Review[J].European J Lipid Sci Technol,2003,105(12):781-782.
[11] 郭金,董锁拽,万和军,等.亚麻纱线USTER测试结果的模糊聚类分析初探[J].现代纺织技术,2009,17(3):44-48.
[12] 胡付品,朱德妹,汪复,等.2015年CHINET细菌耐药性监测[J].中国感染与化疗杂志,2016,16(6):685-694.
[13] 胡付品,郭燕,朱德妹,等.2016年中国CHINET细菌耐药性监测[J].中国感染与化疗杂志,2017,17(5):481-491.
[14] 吴大盈,朱延焱.367株嗜麦芽窄食单胞菌耐药性分析[J].药物流行病学杂志,2014,23(11):664-666.
[15] Falagas ME,Kastoris AC,Vouloumanou EK,et al.Attributable mortality of Stenotrophomonas maltophilia infections:a systematic review of the literature[J].Future Microbiol,2009,4(9):1103-1109.
[16] Huang YW,Hu RM,Lin YT,et al.The contribution of class 1 integron to antimicrobial resistance in Stenotrophomonas maltophilia[J].Microb Drug Resist,2015,21(1):90-96.
[17] Brooke JS.Stenotrophomonas maltophilia:an emerging global opportunistic pathogen[J].Clin Microbiol Rev,2012,25(1):2-41.
[18] 储从家,吴惠玲.445株嗜麦芽窄食单胞菌的分布及耐药性分析[J].中国微生态学杂志,2014,26(8):928-930.
[19] Zhang SK,Yin YL,Jones MB,et al.Salmonella serotype determination utilizing high-throughput genome sequencing data[J].J Clin Microbiol,2015,53(5):1685-1692.
[20] 陈经雕,邓小玲,柯昌文,等.广东省布鲁氏菌菌株脂肪酸成分分析[J].中国人兽共患病学报,2010,26(2):131-133.
[21] 狄慧玲,张耀祺,单潇潇,等.河北地区食源性单核细胞增生李斯特菌脂肪酸的分型研究[J].现代食品科技,2014,30(11):65-70.