不同杜仲单株光合特性的比较
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摘要
为筛选出光合作用强、生长旺盛的优良杜仲单株,对其不同生长期的光合特性进行了测定,并分析了各单株光合参数和叶绿素荧光参数的差异及动态变化规律。结果表明:不同时期的大部分杜仲单株的光合速率(Pn)、蒸腾速率(Tr)与气孔导度(Gs)呈现先升高后下降的单峰趋势,而PSⅡ原初光能转换效率(Fv/Fm)与细胞间CO2质量浓度(Ci)则呈现U型趋势;不同杜仲单株各光合荧光参数均存在极显著差异。通过主成分分析和综合评价结果,初步筛选出N24、N30、B12、B6、B5、N25、N22、B27、N23、B26等10株杜仲实生苗单株为优良单株,其光合特性指标优异,可以作为进一步选育的材料。
In order to screen out the excellent Eucommia ulmoides with strong photosynthesis and vigorous growth,the photosynthetic characteristics at different growth stages were measured.The differences and dynamic changes of photosynthetic parameters and chlorophyll fluorescence parameters of each individual plant were analyzed.The results indicate that the photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of most Eucommia ulmoides plants at different stages show a single peak trend of first increasing and then decreasing,while the primary light energy conversion efficiency(Fv/Fm)and intercellular CO2 concentration(Ci)of PSⅡshow a U-shaped trend.There are significant differences in photosynthetic fluorescence parameters among different Eucommia ulmoides plants.Through principal component analysis and comprehensive evaluation,10 seedlings of Eucommia ulmoides were preliminarily screened as excellent single plants,including the N24,N30,B12,B6,B5,N25,N22,B27,N23,and B26.Their photosynthetic characteristics are excellent and can be used for further breeding.
In order to screen out the excellent Eucommia ulmoides with strong photosynthesis and vigorous growth,the photosynthetic characteristics at different growth stages were measured.The differences and dynamic changes of photosynthetic parameters and chlorophyll fluorescence parameters of each individual plant were analyzed.The results indicate that the photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of most Eucommia ulmoides plants at different stages show a single peak trend of first increasing and then decreasing,while the primary light energy conversion efficiency(Fv/Fm)and intercellular CO2 concentration(Ci)of PSⅡshow a U-shaped trend.There are significant differences in photosynthetic fluorescence parameters among different Eucommia ulmoides plants.Through principal component analysis and comprehensive evaluation,10 seedlings of Eucommia ulmoides were preliminarily screened as excellent single plants,including the N24,N30,B12,B6,B5,N25,N22,B27,N23,and B26.Their photosynthetic characteristics are excellent and can be used for further breeding.
引文
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[5]张峰,上官铁梁.山西翅果油树群落种间关系的数量分析[J].植物生态学报,2000,24(3):351-355.ZHANG Feng,SHANGGUAN Tieliang.Numerical analysis of interspecific relationships in an Elaeagnus mollis community in Shanxi[J].Acta Phytoecologica Sinica,2000,24(3):351-355.(in Chinese)
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[12]POSADA J M,LECHOWICZ M J,KITAJIMA K.Optimal photosynthetic use of light by tropical tree crowns achieved by a djustment of individual leaf angles and nitrogen content[J].Annals of Botany,2009,103(5):795-805.
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[14]朱景乐,杨超伟,杜红岩,等.2个杜仲无性系光合能力比较[J].浙江农林大学学报,2014,31(5):704-709.ZHU Jingle,YANG Chaowei,DU Hongyan,et al.Photosynthetic capacity of two Eucommia ulmoides clones[J].Journal of Zhejiang A&F University,2014,31(5):704-709.(in Chinese)
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[16]朱景乐,杨超伟,杜红岩,等.2个杜仲无性系叶绿素荧光参数对比[J].河南农业大学学报,2014,48(5):575-578.ZHU Jingle,YANG Chaowei,DU Hongyan1,et al.Comparison of chlorophyll fluorescence parameters between two Eucommia ulmoides clones[J].Journal of Henan Agricultural University,2014,48(5):575-578.(in Chinese)
[17]郭连旺,沈允钢,武海,等.杜仲光合特性的研究[J].植物学报,1996,38(4):283-286.GUO Lianwang,SHEN Yungang,WU Hai,et al.Study on the photosynthetic characteristics of Eucommia ulmoides leaves[J].Acta Botanica Sinica,1996,38(4):283-286.(in Chinese)
[18]李蕾,张伟超,郭婧,等.杜仲四倍体与二倍体光合特性比较[J].北方园艺,2012(6):35-38.LI Lei,ZHANG Weichao,GUO Jing,et al.Photosynthetic characteristics of tetraploid and diploid Eucommia ulmoides[J].Northern Horticulture,2012(6):35-38.(in Chinese)
[19]王茹.杜仲光合生理和蒸腾特性的研究[D].合肥:安徽农业大学,2012.WANG Ru.Photosynthetic and transpiration characteristics of Eucommia ulmoides[D].Hefei:Anhui Agricultural University,2012.(in Chinese)
[20]刘振业,刘贞琦.作物光合作用的遗传与育种研究进展[J].贵州农学院学报,1991,19(2):20-38.LIU Zhenye,LIU Zhenqi.The prospect of genetics and breeding of photosynthesis in crops[J].Journal of Guizhou Agricultural College,1991,19(2):20-38.(in Chinese)
[21]林海明,杜子芳.主成分分析综合评价应该注意的问题[J].统计研究,2013,30(8):25-31.LIN Haiming,DU Zifang.Some problems in comprehensive evaluation in the principal component analysis[J].Statistical Research,2013,30(8):25-31.(in Chinese)
[22]黄安,杨联安,杜挺,等.基于主成分分析的土壤养分综合评价[J].干旱区研究,2014,31(5):819-825.HUANG An,YANG Lian’an,DU Ting,et al.Comprehensive assessment of soil nutrients based on PCA[J].Arid Zone Research,2014,31(5):819-825.(in Chinese)
[23]张云,夏国华,马凯,等.遮阴对堇叶紫金牛光合特性和叶绿素荧光参数的影响[J].应用生态学报,2014,25(7):1940-1948.ZHANG Yun,XIA Guohua,MA Kai.et al.Effects of shade on photosynthetic characteristics and chlorophyll fluorescence of Ardisia violacea[J].Chinese Journal of Applied Ecology,2014,25(7):1940-1948.(in Chinese)
[24]张敏,孙西欢,郭向红,等.蓄水坑灌下苹果树光合日变化与影响因子的分析[J].中国科技论文,2015,10(9):1095-1100.ZHANG Min,SUN Xihuan,GUO Xianghong,et al.The analysis of apple diurnal variation of photosynthesis and impact factors under water storage pits irrigation[J].China Sciencepaper,2015,10(9):1095-1100.(in Chinese)
[25]LONG S P,HUMPHRIES S.Photoinhibition of photosynthesis in nature[J].Aannual Review of Plant Biology,1994,45(1):633-662.
[26]刘茜,李勇.不同类型水稻品种之间光合作用的差异研究[J].中国科技论文,2017,12(6):680-685.LIU Xi,LI Yong.Study on the different of photosynthesis among different rice varieties[J].China Sciencepaper,2017,12(6):680-685.(in Chinese)
[27]翟金兰,董娟娥,张博勇,等.杜仲优系有效成分性状稳定性与增长率的研究[J].西北植物学报,2003,23(2):274-279.ZHAI Jinlan,DONG Juane,ZHANG Boyong,et al.Study of character stability and increase-rate of bio-active constituents in chosen clones of Eucommia ulmoides[J].Acta Botanica Boreali-Occidentalia Sinica,2003,23(2):274-279.(in Chinese)
[28]佘诚棋.青钱柳种子形态特征、苗期性状及叶片化学成分的研究[D].南京:南京林业大学,2008.SHE Chengqi.Studies on seed morphologic charaeteristics,seeding characters and chemical copositions of leaf in Cyclocaryapaliurus[D].Nanjing:Nanjing Forestry University,2008.(in Chinese)
[29]刘奇峰,梁宗锁,蔡靖,等.4种药用植物光合特性的研究[J].西北林学院学报,2007,22(6):10-13.LIU Qifeng,LIANG Zongsuo,CAI Jing,et al.Photosynthetic characteristics of four medical plants[J].Journal of Northwest Forestry University,2007,22(6):10-13.(in Chinese)
[30]王森,晏巢.南方鲜食枣2种类型枣吊光合产物积累能力的比较[J].林业科学,2014,50(6):90-97.WANG Sen,YAN Chao.Comparison of the accumulation ability of photosynthetic product between two types of bearing shoots of southern China fresh-eat jujube[J].Scientia Silvae Sinicae,2014,50(6):90-97.(in Chinese)
[31]贺安娜,王李雪,伍贤进,等.显脉旋覆花不同月份光合特性及药用成分含量比较[J].中药材,2015,38(10):2026-2030.HE Anna,WANG Lixue,WU Xianjin,et al.Comparative study on photosynthetic characteristics and medicinal ingredients in different months of Inulanervosa[J].Journal of Chinese Medicinal Materials,2015,38(10):2026-2030.(in Chinese)
[32]谷荣辉,洪利亚,龙春林.植物细胞培养生产次生代谢物的途径[J].植物生理学报,2013,49(9):869-881.GU Ronghui,HONG Liya,LONG Chunlin.The ways of producing secondary metabolites via plant cell culture[J].Plant Physiology Journal,2013,49(9):869-881.(in Chinese)
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[2]杜红岩,刘攀峰,孙志强,等.我国杜仲产业发展布局探讨[J].经济林研究,2012,30(3):130-133,144.DU Hongyan,LIU Panfeng,SUN Zhiqiang,et al.Layout of Eucommia ulmoides industry development in China[J].Nonwood Forest Research,2012,30(3):130-133,144.(in Chinese)
[3]康向阳.杜仲良种选育研究现状及展望[J].北京林业大学学报,2017,39(3):1-6.KANG Xiangyang.Status and prospect of improved variety selection in Eucommia ulmoides[J].Journal of Beijing Forestry University,2017,39(3)1-6.(in Chinese)
[4]何景峰.陕西杜仲研究、开发现状及对策[J].西北林学院学报,2000,15(1):64-68.HE Jingfeng.Current status and prospects of research and exploitation on Eucommia ulmoides in Shaanxi[J].Journal of Northwest Forestry University,2000,15(1):64-68.(in Chinese)
[5]张峰,上官铁梁.山西翅果油树群落种间关系的数量分析[J].植物生态学报,2000,24(3):351-355.ZHANG Feng,SHANGGUAN Tieliang.Numerical analysis of interspecific relationships in an Elaeagnus mollis community in Shanxi[J].Acta Phytoecologica Sinica,2000,24(3):351-355.(in Chinese)
[6]周先叶,王伯荪,李鸣光,等.广东黑石顶自然保护区森林次生演替过程中群落的种间联结性分析[J].植物生态学报,2000,24(3):332-339.ZHOU Xianye,WANG Bosun,LI Mingguang,et al.An analysis of interspecific associations in secondary succession forest communities in Heishiding natural reserve,Guangdong province[J].Acta Phytoecologica Sinica,2000,24(3):332-339.(in Chinese)
[7]NAVARRO-SAMPEDRO L,YANOFSKY C,COR-ROCHANO L M.A genetic selection for Neurospora crassa mutants altered in their light regulation of transcription[J].Genetics,2008,178(1):171-183.
[8]李鹏民,高辉远,STRASSER R J.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566.LI Pengmin,GAO Huiyuan,STRASSER R J.Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study[J].Journal of Plant Physiology and Molecular Biology,2005,31(6):559-566.(in Chinese)
[9]GENARD M,BARET F,SIMON D.A 3Dpeach canopy model used to evaluate the effect of tree architecture and density on photosynthesis at a range of scales[J].Ecological Modelling,2000,128(2/3):197-209.
[10]张国显,范永怀,赵凤艳,等.化肥减量配施有机物料对设施番茄生长、光合特性、产量及品质的影响[J].中国科技论文,2018,13(6):698-703.ZHANG Guoxian,FAN Yonghuai,ZHAO Fengyan,et al.Effects of organic materials combined application with mineral fertilizer on the growth,photosynthesis,yield and quality of tomato in greenhouse[J].China Sciencepaper,2018,13(6):698-703.(in Chinese)
[11]IZIOMON M G,MAYER H.Performance of solar radiation model-a case study[J].Agricultural and Forest Meteorology,2001,110(1):1-11.
[12]POSADA J M,LECHOWICZ M J,KITAJIMA K.Optimal photosynthetic use of light by tropical tree crowns achieved by a djustment of individual leaf angles and nitrogen content[J].Annals of Botany,2009,103(5):795-805.
[13]RASZEWSKI G,SAENGER W,RENGER T.Theory of optical spectra of photosystemⅡreaction centers:location of the triplet state and the identity of the primary electron donor[J].Biophysical Journal,2005,88(2):986-998.
[14]朱景乐,杨超伟,杜红岩,等.2个杜仲无性系光合能力比较[J].浙江农林大学学报,2014,31(5):704-709.ZHU Jingle,YANG Chaowei,DU Hongyan,et al.Photosynthetic capacity of two Eucommia ulmoides clones[J].Journal of Zhejiang A&F University,2014,31(5):704-709.(in Chinese)
[15]吕宁.几个杜仲无性系光合特性比较研究[J].陕西林业科技,2016(5):1-6.LNing.Photosynthesis characteristics of clones of Eucommia ulmoides[J].Shaanxi Forest Science and Technology,2016(5):1-6.(in Chinese)
[16]朱景乐,杨超伟,杜红岩,等.2个杜仲无性系叶绿素荧光参数对比[J].河南农业大学学报,2014,48(5):575-578.ZHU Jingle,YANG Chaowei,DU Hongyan1,et al.Comparison of chlorophyll fluorescence parameters between two Eucommia ulmoides clones[J].Journal of Henan Agricultural University,2014,48(5):575-578.(in Chinese)
[17]郭连旺,沈允钢,武海,等.杜仲光合特性的研究[J].植物学报,1996,38(4):283-286.GUO Lianwang,SHEN Yungang,WU Hai,et al.Study on the photosynthetic characteristics of Eucommia ulmoides leaves[J].Acta Botanica Sinica,1996,38(4):283-286.(in Chinese)
[18]李蕾,张伟超,郭婧,等.杜仲四倍体与二倍体光合特性比较[J].北方园艺,2012(6):35-38.LI Lei,ZHANG Weichao,GUO Jing,et al.Photosynthetic characteristics of tetraploid and diploid Eucommia ulmoides[J].Northern Horticulture,2012(6):35-38.(in Chinese)
[19]王茹.杜仲光合生理和蒸腾特性的研究[D].合肥:安徽农业大学,2012.WANG Ru.Photosynthetic and transpiration characteristics of Eucommia ulmoides[D].Hefei:Anhui Agricultural University,2012.(in Chinese)
[20]刘振业,刘贞琦.作物光合作用的遗传与育种研究进展[J].贵州农学院学报,1991,19(2):20-38.LIU Zhenye,LIU Zhenqi.The prospect of genetics and breeding of photosynthesis in crops[J].Journal of Guizhou Agricultural College,1991,19(2):20-38.(in Chinese)
[21]林海明,杜子芳.主成分分析综合评价应该注意的问题[J].统计研究,2013,30(8):25-31.LIN Haiming,DU Zifang.Some problems in comprehensive evaluation in the principal component analysis[J].Statistical Research,2013,30(8):25-31.(in Chinese)
[22]黄安,杨联安,杜挺,等.基于主成分分析的土壤养分综合评价[J].干旱区研究,2014,31(5):819-825.HUANG An,YANG Lian’an,DU Ting,et al.Comprehensive assessment of soil nutrients based on PCA[J].Arid Zone Research,2014,31(5):819-825.(in Chinese)
[23]张云,夏国华,马凯,等.遮阴对堇叶紫金牛光合特性和叶绿素荧光参数的影响[J].应用生态学报,2014,25(7):1940-1948.ZHANG Yun,XIA Guohua,MA Kai.et al.Effects of shade on photosynthetic characteristics and chlorophyll fluorescence of Ardisia violacea[J].Chinese Journal of Applied Ecology,2014,25(7):1940-1948.(in Chinese)
[24]张敏,孙西欢,郭向红,等.蓄水坑灌下苹果树光合日变化与影响因子的分析[J].中国科技论文,2015,10(9):1095-1100.ZHANG Min,SUN Xihuan,GUO Xianghong,et al.The analysis of apple diurnal variation of photosynthesis and impact factors under water storage pits irrigation[J].China Sciencepaper,2015,10(9):1095-1100.(in Chinese)
[25]LONG S P,HUMPHRIES S.Photoinhibition of photosynthesis in nature[J].Aannual Review of Plant Biology,1994,45(1):633-662.
[26]刘茜,李勇.不同类型水稻品种之间光合作用的差异研究[J].中国科技论文,2017,12(6):680-685.LIU Xi,LI Yong.Study on the different of photosynthesis among different rice varieties[J].China Sciencepaper,2017,12(6):680-685.(in Chinese)
[27]翟金兰,董娟娥,张博勇,等.杜仲优系有效成分性状稳定性与增长率的研究[J].西北植物学报,2003,23(2):274-279.ZHAI Jinlan,DONG Juane,ZHANG Boyong,et al.Study of character stability and increase-rate of bio-active constituents in chosen clones of Eucommia ulmoides[J].Acta Botanica Boreali-Occidentalia Sinica,2003,23(2):274-279.(in Chinese)
[28]佘诚棋.青钱柳种子形态特征、苗期性状及叶片化学成分的研究[D].南京:南京林业大学,2008.SHE Chengqi.Studies on seed morphologic charaeteristics,seeding characters and chemical copositions of leaf in Cyclocaryapaliurus[D].Nanjing:Nanjing Forestry University,2008.(in Chinese)
[29]刘奇峰,梁宗锁,蔡靖,等.4种药用植物光合特性的研究[J].西北林学院学报,2007,22(6):10-13.LIU Qifeng,LIANG Zongsuo,CAI Jing,et al.Photosynthetic characteristics of four medical plants[J].Journal of Northwest Forestry University,2007,22(6):10-13.(in Chinese)
[30]王森,晏巢.南方鲜食枣2种类型枣吊光合产物积累能力的比较[J].林业科学,2014,50(6):90-97.WANG Sen,YAN Chao.Comparison of the accumulation ability of photosynthetic product between two types of bearing shoots of southern China fresh-eat jujube[J].Scientia Silvae Sinicae,2014,50(6):90-97.(in Chinese)
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