超高压液相色谱-四级杆-飞行时间质谱仪的管理、维护与故障处理
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摘要
超高压液相色谱-四级杆-飞行时间串联质谱仪是分离鉴定复杂有机物组分的重要工具,如何使仪器保持良好的运行状态,同时延长其使用寿命、节约耗材、降低运行成本是至关重要的.对仪器的日常管理、维护与常见故障处理进行了总结介绍.
Ultra-high performance liquid chromatograph tandem quadrupole time of flight mass spectrometer(UHPLC-Q-TOF MS) is an important tool for the separation and identification of complex organic compounds. How to keep the instrument in good working condition and prolong its service life, save material and reduce operation cost is very important. The daily management, maintenance and common troubleshooting of the instrument are summarized and introduced.
Ultra-high performance liquid chromatograph tandem quadrupole time of flight mass spectrometer(UHPLC-Q-TOF MS) is an important tool for the separation and identification of complex organic compounds. How to keep the instrument in good working condition and prolong its service life, save material and reduce operation cost is very important. The daily management, maintenance and common troubleshooting of the instrument are summarized and introduced.
引文
[1] Jungfer E, Zimmermann B F, Ruttkat A, et al. Comparing procyanidins in selected vaccinium species by UHPLC-MS with regard to authenticity and health effects[J]. J Agr Food Chem, 2012, 60(38): 9688-9696.
[2] 闫清华. 超高效液相色谱-串联质谱法测定水产品中孔雀石绿前处理方法的改进[J]. 分析测试技术与仪器, 2018, 24(3): 179-182. [YAN Qing-hua. Improvement of malachite green pretreatment in aquatic products by UPLC-MS/MS[J]. Analysis and Testing Technology and Instruments, 2018, 24(3): 179-182.]
[3] 颜晓丽. 超高效液相色谱-串联质谱法测定食品中4种罂粟壳生物碱[J]. 分析测试技术与仪器, 2017, 23(1): 11-17. [YAN Xiao-li. Simultaneous determination of four opium poppy husk alkaloids in residues of foods using ultra performance liquid chromatography-tandem mass spectrometry[J]. Analysis and Testing Technology and Instruments, 2017, 23(1): 11-17.]
[4] 姚慧, 刘小花, 陈心悦, 等. UPLC-ESI-MS法测定贞芪扶正胶囊中毛蕊异黄酮-7-O-β-D-葡萄糖苷、红景天苷和黄芪甲苷的含量[J]. 分析测试技术与仪器, 2016, 22(4): 204-208. [YAO Hui, LIU Xiao-hua, CHEN Xin-yue, et al. UPLC-ESI-MS method for rapid quantification of rhodioside, calycosin-7-O-β-D-glucosideand astragaloside IV in Zhenqi Fuzheng capsule[J]. Analysis and Tesing Technology and Instruments, 2016, 22(4): 204-208.]
[5] 孙琪, 陈帆. 一种测定植物甾醇的高灵敏度衍生化HPLC-MS/MS方法[J]. 分析测试技术与仪器, 2016, 22(3): 169-178. [SUN Qi, CHEN Fan. A highly sensitive method for quantitative analysis of phytosterols by HPLC-MS/MS with pre-column derivatization[J]. Analysis and Testing Technology and Instruments, 2016, 22(3): 169-178.]
[2] 闫清华. 超高效液相色谱-串联质谱法测定水产品中孔雀石绿前处理方法的改进[J]. 分析测试技术与仪器, 2018, 24(3): 179-182. [YAN Qing-hua. Improvement of malachite green pretreatment in aquatic products by UPLC-MS/MS[J]. Analysis and Testing Technology and Instruments, 2018, 24(3): 179-182.]
[3] 颜晓丽. 超高效液相色谱-串联质谱法测定食品中4种罂粟壳生物碱[J]. 分析测试技术与仪器, 2017, 23(1): 11-17. [YAN Xiao-li. Simultaneous determination of four opium poppy husk alkaloids in residues of foods using ultra performance liquid chromatography-tandem mass spectrometry[J]. Analysis and Testing Technology and Instruments, 2017, 23(1): 11-17.]
[4] 姚慧, 刘小花, 陈心悦, 等. UPLC-ESI-MS法测定贞芪扶正胶囊中毛蕊异黄酮-7-O-β-D-葡萄糖苷、红景天苷和黄芪甲苷的含量[J]. 分析测试技术与仪器, 2016, 22(4): 204-208. [YAO Hui, LIU Xiao-hua, CHEN Xin-yue, et al. UPLC-ESI-MS method for rapid quantification of rhodioside, calycosin-7-O-β-D-glucosideand astragaloside IV in Zhenqi Fuzheng capsule[J]. Analysis and Tesing Technology and Instruments, 2016, 22(4): 204-208.]
[5] 孙琪, 陈帆. 一种测定植物甾醇的高灵敏度衍生化HPLC-MS/MS方法[J]. 分析测试技术与仪器, 2016, 22(3): 169-178. [SUN Qi, CHEN Fan. A highly sensitive method for quantitative analysis of phytosterols by HPLC-MS/MS with pre-column derivatization[J]. Analysis and Testing Technology and Instruments, 2016, 22(3): 169-178.]