miR-143、miR-145联合血清AFP检测对原发性肝癌的临床诊断价值
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摘要
目的探讨miR-143、miR-145联合血清甲胎蛋白(alpha-feto protein, AFP)对原发性肝癌的临床诊断价值。方法选取2016年7月—2017年9月我院收治的原发性肝癌60例作为肝癌组,取同期肝硬化60例作为肝硬化组、健康体检者60例作为健康对照组。比较3组miR-143、miR-145、AFP水平,计算不同检查方式诊断原发性肝癌的灵敏度及特异度。结果肝癌组miR-143、miR-145、血清AFP水平高于肝硬化组和健康对照组(P<0.05)。miR-143+miR-145+AFP诊断原发性肝癌的灵敏度及特异度高于AFP、miR-145+AFP、miR-143+AFP单一诊断(P<0.05)。结论 miR-143、miR-145在原发性肝癌患者血清中表达水平升高,联合AFP检测可提高诊断原发性肝癌的灵敏度及特异度。
Objective To investigate clinical value of miR-143 and miR-145 combined with serum alpha-fetoprotein(AFP) in diagnosis of patients with primary liver cancer. Methods A total of 60 patients with primary liver cancer admitted during July 2016 and September 2017 were selected as liver cancer group, and another 60 patients with liver cirrhosis at the same period were selected as liver cirrhosis group, and 60 healthy people taking physical examination were selected as control group. Among three groups, levels of miR-143, miR-145 and AFP were compared, and sensitivity and specificity values of different examination methods in diagnosis of patients with primary liver cancer were calculated respectively. Results Levels of miR-143, miR-145 and AFP in liver cancer group were significantly higher than those in liver cirrhosis and healthy control groups(P<0.05). Sensitivity and specificity values of miR-143 + miR-145 +AFP in diagnosis of patients with primary liver cancer were significantly higher than those by AFP, miR-145 +AFP and miR-143 +AFP alone(P<0.05). Conclusion Expressions of miR-143 and miR-145 in serum of patients with primary liver cancer are increased, and combined detection of AFP may improve sensitivity and specificity in diagnosis of patients with primary liver cancer.
Objective To investigate clinical value of miR-143 and miR-145 combined with serum alpha-fetoprotein(AFP) in diagnosis of patients with primary liver cancer. Methods A total of 60 patients with primary liver cancer admitted during July 2016 and September 2017 were selected as liver cancer group, and another 60 patients with liver cirrhosis at the same period were selected as liver cirrhosis group, and 60 healthy people taking physical examination were selected as control group. Among three groups, levels of miR-143, miR-145 and AFP were compared, and sensitivity and specificity values of different examination methods in diagnosis of patients with primary liver cancer were calculated respectively. Results Levels of miR-143, miR-145 and AFP in liver cancer group were significantly higher than those in liver cirrhosis and healthy control groups(P<0.05). Sensitivity and specificity values of miR-143 + miR-145 +AFP in diagnosis of patients with primary liver cancer were significantly higher than those by AFP, miR-145 +AFP and miR-143 +AFP alone(P<0.05). Conclusion Expressions of miR-143 and miR-145 in serum of patients with primary liver cancer are increased, and combined detection of AFP may improve sensitivity and specificity in diagnosis of patients with primary liver cancer.
引文
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[17] 王锋,秦叔逵,方维佳,等.抗PD-1单抗SHR-1210治疗原发性肝癌引发皮肤毛细血管增生症的临床病理报告[J].临床肿瘤学杂志,2017,22(12):1066-1072.
[18] 吴伟慎,赵莹,何海艳,等.天津市乙型和丙型病毒性肝炎相关肝硬化肝癌死亡率及流行特征研究[J].预防医学情报杂志,2016,32(9):941-944.
[19] 李京源,李涛,朱席琳,等.ADAR1通过RNA编辑上调ZNF655表达并促进人肝癌细胞系HepG2中HBV复制[J].基础医学与临床,2018,38(3):312-316.
[20] 潘佳倩,童双梅,汤静.La以及La相关蛋白在肝癌细胞中的表达和意义[J].医学分子生物学杂志,2018,41(1):8-12.
[21] 王建,陈小芳,钟训富,等.2010-2014年彭州市户籍人口恶性肿瘤发病与死亡分析[J].职业卫生与病伤,2017,31(6):345-350.
[22] 张引,余力信,张发鹏,等.趋化因子受体CXCR4及其配体CXCL12在肝癌侵袭转移中的作用及机制[J].天津医药,2018,46(1):20-26.
[23] 张志梅,何晓燕,施瑞华.miR-143在食管鳞癌和上皮内瘤变组织中的表达及其临床意义[J].胃肠病学,2017,22(6):364-366.
[24] 王欣怡,张海洋,李爽,等.miR-143抑制胃癌细胞SGC7901增殖与迁移的机制研究[J].中国肿瘤临床,2016,43(16):702-706.
[25] 梁亮,张寅斌,昝瑛,等.肝细胞癌组织miR-139水平及其靶基因功能的生物信息学分析[J].实用肝脏病杂志,2017,20(5):554-558.
[2] 蔡洁新,杨立杰,赵振杰.原发性肝癌病人AFP-L3、AFU、GP73及TSGF等血清标志物的水平分析及临床意义[J].标记免疫分析与临床,2016,23(12):1409-1412.
[3] 王宇洲,许建华,常伟华,等.长链非编码RNALOC100288637在原发性肝癌中差异性表达的生物学意义及相关机制研究[J].重庆医学,2016,45(16):2198-2201.
[4] 宋巍.联合检测血清AFP、AFU、GP73及IL-8在原发性肝癌诊断中的临床意义[J].中华临床医师杂志:电子版,2016,10(9):1266-1270.
[5] 宋冉,周磊.S-腺苷蛋氨酸对原发性肝癌患者术后肝损伤保护作用及机制研究[J].中国临床药理学杂志,2018,34(6):1292-1294.
[6] Slaby O,Svoboda M,Fabian P,et al.Altered expression of miR-21,miR-31,miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer[J].Oncology,2007,72(5-6):397-402.
[7] 谭式辉.血清miR-143和miR-145水平诊断肝癌的临床价值研究[D].南宁:广西医科大学,2017.
[8] 王晓燕.联合检测血清GP73、AFP及AFP-L3在原发性肝癌中的诊断价值[J].标记免疫分析与临床,2017,24(1):64-66.
[9] 罗汝琼,唐亮,谢萍,等.宫颈癌患者HPV DNA病毒含量及循环血、组织miR指标的变化研究[J].海南医学院学报,2016,22(16):1841-1843.
[10] 魏巍,熊诗诗,郭志华,等.Notch通路介导的microRNAs对肝癌干细胞的调节作用[J].医学分子生物学杂志,2017,14(5):295-300.
[11] 何华,黄毓,杨定华,等.miR-126/miR-126在肝细胞癌中的表达及其与临床预后的相关性[J].南方医科大学学报,2014,34(10):1493-1497.
[12] 李明,李庆军,周进学,等.miR-103通过抑制LATS2促进肝细胞肝癌侵袭转移的机制[J].肝胆外科杂志,2017,25(4):312-316.
[13] 张晓云,王立峰.miR-143在结肠癌组织中的差异表达及其对结肠癌细胞凋亡和迁移的影响[J].上海交通大学学报:医学版,2017,37(3):325-329.
[14] Johannessen C,Moi L,Kiselev Y,et al.Expression and function of the miR-143/145 cluster in vitro and in vivo in human breast cancer[J].PLoS One,2017,12(10):e0186658.
[15] 谢云,田洲,渠亚超,等.血清microRNA-143在乙型肝炎病毒相关性肝病中的表达和临床意义[J].中华临床医师杂志:电子版,2013,7(24):11370-11375.
[16] 董慧.优质护理在行TAE治疗的原发性肝癌患者护理中的应用效果[J].保健医学研究与实践,2017,14(4):77-78,90.
[17] 王锋,秦叔逵,方维佳,等.抗PD-1单抗SHR-1210治疗原发性肝癌引发皮肤毛细血管增生症的临床病理报告[J].临床肿瘤学杂志,2017,22(12):1066-1072.
[18] 吴伟慎,赵莹,何海艳,等.天津市乙型和丙型病毒性肝炎相关肝硬化肝癌死亡率及流行特征研究[J].预防医学情报杂志,2016,32(9):941-944.
[19] 李京源,李涛,朱席琳,等.ADAR1通过RNA编辑上调ZNF655表达并促进人肝癌细胞系HepG2中HBV复制[J].基础医学与临床,2018,38(3):312-316.
[20] 潘佳倩,童双梅,汤静.La以及La相关蛋白在肝癌细胞中的表达和意义[J].医学分子生物学杂志,2018,41(1):8-12.
[21] 王建,陈小芳,钟训富,等.2010-2014年彭州市户籍人口恶性肿瘤发病与死亡分析[J].职业卫生与病伤,2017,31(6):345-350.
[22] 张引,余力信,张发鹏,等.趋化因子受体CXCR4及其配体CXCL12在肝癌侵袭转移中的作用及机制[J].天津医药,2018,46(1):20-26.
[23] 张志梅,何晓燕,施瑞华.miR-143在食管鳞癌和上皮内瘤变组织中的表达及其临床意义[J].胃肠病学,2017,22(6):364-366.
[24] 王欣怡,张海洋,李爽,等.miR-143抑制胃癌细胞SGC7901增殖与迁移的机制研究[J].中国肿瘤临床,2016,43(16):702-706.
[25] 梁亮,张寅斌,昝瑛,等.肝细胞癌组织miR-139水平及其靶基因功能的生物信息学分析[J].实用肝脏病杂志,2017,20(5):554-558.
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