TLC-HPLC法检测减肥产品中的西地那非和西布曲明

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英文篇名：Rapid Detection of Sildenafil and Sibutramine in Weight-loss Products by TLC-HPLC 作者：黄涛 ; 曾祥 ; 陈静 ; 陈瑾 ; 陈聪聪 ; 曹晓琴 英文作者：HUANG Tao;ZENG Xiang;CHEN Jing;CHEN Jin;CHEN Congcong;CAO Xiaoqin;Jianghan University; 关键词：西地那非 ; 西布曲明 ; 高效液相色谱 ; 减肥类保健品 英文关键词：sildena?l;;sibutramine;;HPLC;;weight-loss 中文刊名：GXHG 英文刊名：Technology & Development of Chemical Industry 机构：江汉大学; 出版日期：2019-02-15 出版单位：化工技术与开发 年：2019 期：v.48;No.297 基金：湖北省教育厅科学研究计划项目(B2016288);; 2017年大学生科研校级重点项目 语种：中文; 页：GXHG201902006 页数：3 CN：02 ISSN：45-1306/TQ 分类号：25-27

摘要

建立了薄层色谱法-高效液相色谱法快速测定减肥类保健品中西地那非和西布曲明等成分的方法。采用薄层色谱法(TLC)进行前处理,配合高效液相色谱法,C18色谱柱,流动相为甲醇/三乙胺(100/0.1),用冰醋酸调节pH=7,检测波长254 nm,流速1.0mL·min~(-1)进行检测。在选定的色谱条件下,2种非法添加的化学药物成分有很好的分离,西布曲明和西地那非均在5～100μg·mL~(-1)范围内线性关系良好,相关系数(R2)≥0.9997。不同样品中的西地那非和西布曲明的最低检测限分别为4.2μg·mL~(-1)和3.1μg·mL~(-1)。该方法灵敏准确,可用于减肥类保健品的质量检验监督。
        A HPLC method was developed for the rapid determination of sildena?l and sibutramine in weight-loss.TLC method was used for pretreatment,combined with high performance liquid chromatography and C18 column,mobile phase was methanol/three ethylamine(100/0.1),using glacial acetic acid to regulate pH=7.The detection wave length was 254 nm and the ?ow rate was 1.0 mL/min.Under selected chromatographic conditions,2 kinds of illegally added chemical ingredients were well separated.The method was sensitive and accurate,and could be used for the quality control of health care products.

引文

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