正交试验法优选蒲葵子多糖的提取工艺
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摘要
目的建立水提醇沉法提取蒲葵子多糖的最佳提取工艺。方法采用正交试验,以多糖含量为指标,优选提取时间(A)、提取次数(B)、粒度(C)。结果影响蒲葵子多糖含量的因素主次关系C>A>B,最佳提取工艺为A2B3C2。结论药材粒度对结果有显著影响,从经济、省时的角度考虑,生产上应把药材的粒度作为关键性因素考虑。
Objective To set up the optimum extraction technology of polysaccharide from Livistonae chinensis fructus by water extraction and alcohol precipitation. Methods By using orthogonal test method, with the content of polysaccharide as the index, the extraction time(A), extraction times(B), and the particle size of the medicinal material(C) were optimized. Results The order of the influencing factors on the content of polysaccharide from Livistonae chinensis fructus was as follows: C>A>B. The optimum extraction technology was A2B3C2. Conclusion The particle size shows remarkable influence on the extraction results. From the perspective of cost reduction and time efficiency, the particle size should be considered as the sticking point on production.
Objective To set up the optimum extraction technology of polysaccharide from Livistonae chinensis fructus by water extraction and alcohol precipitation. Methods By using orthogonal test method, with the content of polysaccharide as the index, the extraction time(A), extraction times(B), and the particle size of the medicinal material(C) were optimized. Results The order of the influencing factors on the content of polysaccharide from Livistonae chinensis fructus was as follows: C>A>B. The optimum extraction technology was A2B3C2. Conclusion The particle size shows remarkable influence on the extraction results. From the perspective of cost reduction and time efficiency, the particle size should be considered as the sticking point on production.
引文
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[2]杨炳友,孟永海,宋佳欣,等.吴茱萸多糖水提工艺优化研究[J].中医药学报,2012,40(2):66-69.
[3]佟茵,吴红娟.植物多糖的提取方法研究进展[J].中医药信息,2012,29(5):108-110.
[4]夏泉,谢永忠,黄赵刚,等.正交设计优化黄精多糖的水提醇沉工艺[J].中国药师,2006,(10):905-906.
[5]张惟杰.复合多糖生化研究技术[M].上海:上海科学技术出版社,1987:7.
[6]林颖,吴毓敏,吴雯,等.天然产物中的糖含量测定方法正确性的研究[J].天然产物研究与开发,1996,8(3):6-9.
[7]高续春.多糖提取纯化方法研究进展[J].榆林学院学报,2009,(4):65-67.