龙首参葛丸对血管性痴呆大鼠海马CA1区组织结构变化的影响
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摘要
目的:观察龙首参葛丸对血管性痴呆大鼠海马CA1区组织结构变化的影响。方法:采用改良2-VO法复制血管性痴呆模型大鼠60只,分为正常组、阳性对照组(NS,10 mL/kg),脑复康组(0.8 g/kg),龙首参葛丸低剂量(1.5g/kg)组、中剂量(3g/kg)组、高剂量(6g/kg)组,每组10只。经口灌胃等体积给药,10mL/kg动物体质量,1次/日。在大鼠造模前及造模后第38天、39天、40天进行跳台试验,对大鼠进行学习记忆能力的评估。大鼠于第41天给予10%水合氯醛麻醉迅速断头取脑,取视交叉后4 mm与小脑前之间的部分,经4%多聚甲醛浸置后,制作成厚约5μm的冠状切片,HE染色后镜下观察海马CA1区组织结构变化。结果:跳台试验显示:龙首参葛丸低、中、高剂量组及脑复康组与阳性对照组比较,差异均有统计学意义(P<0.05),说明龙首参葛丸和脑复康都有改善大鼠学习记忆功能的作用,但龙首参葛丸高剂量组相较脑复康组作用更加明显(P<0.05);镜下染色观察:龙首葛根组与脑复康组、阳性对照组比较,大鼠海马区CA1区尼氏体(虎斑样结构)体积大、数量多,且分布较均匀,发生尼氏体溶解的细胞数量也较少。结论:龙首参葛丸可促进神经元内蛋白质合成,减少神经元损伤,促进神经元修复,对血管性痴呆具有治疗和保护作用。
Objective: To observe the effects of LongShou ShenGe pills on the structural changes of the tissue in hippocampal CAI region in the rats with vascular dementia(VD). Methods: sixtyVD rat models were duplicated using modified 2-VO method, and they were randomized into the normal group, positive control group(NS, 10 m L/mg),the group of NaoFuKang(0.8 g/kg), low(1.5 g/kg), moderate(3 g/kg) and high(6 g/kg) doses groups of LongShou ShenGe pills, oral gavage in equal volume, 10 m L/kg, body mass, once each day. Step down test was performed before modeling, at the 38 d, 39 d and 40 d after modeling to assess learning and memory ability. The rats were sacrificed at the 41 st day after giving 10% chloral hydrate, the brain was drawn and the part 4 mm behind chiasma opticum and before the epencephalon were taken, after managed with 4% paraformaldehyde, it was prepared into coronal section, around 5 μm thick, the slices were observed after HE staining. Results: Step down test showed that:the difference showed significant meaning when low, moderate and high doses groups of LongShou ShenGe pills,and NaoFuKang group were compared with positive control group(P<0.05), which demonstrated that LongShou ShenGe pills and NaoFuKang could improve learning and memory ability of the rats, while the effects of high dose group of LongShou ShenGe pills were more notable compared with NaoFuKang group(P<0.05). Observation under microscope after staining: When LongShou GeGen pill group was compared with NaoFuKang group and positive control group, the tigroid body in hippocampal CAI region was in large size, numerous and well-distributed, and the numbers of the cells appearing chromatolysis were fewer. Conclusion: LongShou ShenGe pill could promote protein synthesis in nerve cells, reduce neuron injury and improve the neuron repair, and it shows therapeutic effects and protective effects on VD.
Objective: To observe the effects of LongShou ShenGe pills on the structural changes of the tissue in hippocampal CAI region in the rats with vascular dementia(VD). Methods: sixtyVD rat models were duplicated using modified 2-VO method, and they were randomized into the normal group, positive control group(NS, 10 m L/mg),the group of NaoFuKang(0.8 g/kg), low(1.5 g/kg), moderate(3 g/kg) and high(6 g/kg) doses groups of LongShou ShenGe pills, oral gavage in equal volume, 10 m L/kg, body mass, once each day. Step down test was performed before modeling, at the 38 d, 39 d and 40 d after modeling to assess learning and memory ability. The rats were sacrificed at the 41 st day after giving 10% chloral hydrate, the brain was drawn and the part 4 mm behind chiasma opticum and before the epencephalon were taken, after managed with 4% paraformaldehyde, it was prepared into coronal section, around 5 μm thick, the slices were observed after HE staining. Results: Step down test showed that:the difference showed significant meaning when low, moderate and high doses groups of LongShou ShenGe pills,and NaoFuKang group were compared with positive control group(P<0.05), which demonstrated that LongShou ShenGe pills and NaoFuKang could improve learning and memory ability of the rats, while the effects of high dose group of LongShou ShenGe pills were more notable compared with NaoFuKang group(P<0.05). Observation under microscope after staining: When LongShou GeGen pill group was compared with NaoFuKang group and positive control group, the tigroid body in hippocampal CAI region was in large size, numerous and well-distributed, and the numbers of the cells appearing chromatolysis were fewer. Conclusion: LongShou ShenGe pill could promote protein synthesis in nerve cells, reduce neuron injury and improve the neuron repair, and it shows therapeutic effects and protective effects on VD.
引文
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[12]代建峰,张宾辉.补肾祛瘀开窍方对血管性痴呆大鼠脑组织海马CA1区锥体细胞的影响[J],中国中医药科技2004,11(2):109-110.
[13]王玉良,周永翠,王益光,等.血管性痴呆大鼠海马CA1区神经元超微结构的长时程变化[J].潍坊医学院学报,2008,30(5):389-391.
[14]Liu R,Lei JX,Luo C,et al.Increased EID1 nuclear translocation impairs synaptic plasticity and memory function associated with patho-genesis of Alzheimer′s disease[J].Neurobiol Dis,2012,45(3):902-912.
[15]Pol LVD,Gertz HJ,Scheltens P,et al.Hippocampal atrophy in subcortical vascular dementia[J].Neurodegener Dis,2011,8(6):465-469.
[16]孔令军,房敏,詹红生,等.大鼠腰椎亚脱位模型刚度及其脊髓前角尼氏小体变化研究[J].中华中医药杂志,2012,27(12):3234-3237.
[2]马婧怡,张万鑫,陈虹,等.改良方法制备的血管性痴呆模型大鼠的学习记忆能力表现[J].中国药理学与毒理学杂志,2014,28(3):421-425.
[3]赵建新,田元祥,李国明,等.脑缺血再灌注拟血管性痴呆小鼠皮层及海马细胞病理形态学动态观察[J].中风与神经疾病杂志,2000,17(4):200-202.
[4]Romn GC.Cholinergic dysfunction in vascular dementia[J].Curr Psychiatry Rep,2005,7(1):18-26.
[5]陈燕.神经元的突触可塑性与学习和记忆[J].生物化学与生物物理进展,2008,35(6):610-613.
[6]Wang JY,Xia Q,Chu KT,et al.Severe global cerebral ischemia induced programmed necrosis of hippocampal CAl neurous in rat is prevented by 3-methyladenine:a widely used inhibitor of autophagy[J].Neur Exp Neurol,2011,70(7):314-322.
[7]马献中,薛积才,王保平,等.龙首参葛丸治疗血管性痴呆临床观察[J].新中医,2012,44(12):32-33.
[8]马献中,陈怡名,王保平,等.龙首参葛丸对血管性痴呆大鼠认知功能的改善作用观察[J].中药材,2017,40(1):204-207.
[10]马婧怡,张万鑫,陈虹,等.改良方法制备的血管性痴呆模型大鼠的学习记忆能力表现[J].中国药理学与毒理学杂志,2014,28(3):421-425.
[12]代建峰,张宾辉.补肾祛瘀开窍方对血管性痴呆大鼠脑组织海马CA1区锥体细胞的影响[J],中国中医药科技2004,11(2):109-110.
[13]王玉良,周永翠,王益光,等.血管性痴呆大鼠海马CA1区神经元超微结构的长时程变化[J].潍坊医学院学报,2008,30(5):389-391.
[14]Liu R,Lei JX,Luo C,et al.Increased EID1 nuclear translocation impairs synaptic plasticity and memory function associated with patho-genesis of Alzheimer′s disease[J].Neurobiol Dis,2012,45(3):902-912.
[15]Pol LVD,Gertz HJ,Scheltens P,et al.Hippocampal atrophy in subcortical vascular dementia[J].Neurodegener Dis,2011,8(6):465-469.
[16]孔令军,房敏,詹红生,等.大鼠腰椎亚脱位模型刚度及其脊髓前角尼氏小体变化研究[J].中华中医药杂志,2012,27(12):3234-3237.
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