通过外源MeJA抑制H_2O_2积累提高烟草的耐冷性
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摘要
茉莉酸甲酯(MeJA)是可参与多种生理生化过程的激发子,为探究外源MeJA对低温环境下烟草幼苗的影响,以烟草品种"豫烟10号"为材料,在其六叶一心时用4个不同浓度(1、10、100和1000μmol L~(–1) MeJA)进行喷施处理3d后,再进行低温处理,同时以正常温度和低温处理作为阳性和阴性对照。分析各处理的长势指标、相对电解质渗透率、光合色素含量、抗氧化酶活性以及激素含量。结果表明:在10μmol L~(–1)茉莉酸甲酯处理下能降低低温对烟草幼苗的损伤。然后在材料和时期不变的情况下,进行DPI、10μmol L~(–1) MeJA以及DPI+MeJA处理以低温处理为对照,测定了H_2O_2、O~(2–)、CAT、MDA以及ASA-GSH循环的含量。证明在外源茉莉酸甲酯协同低温的处理下,烟草植株中的H_2O_2主要作为毒害分子而非第二信使存在。
Methyl jasmonate(MeJA) is an elicitor evolving in a variety of physiological and biochemical processes. To explore the effects of exogenous MeJA on tobacco seedlings under low temperature conditions, we used the tobacco variety "Yuyan 10" and sprayed four concentrations(1, 10, 100, 1000 μmol L~(–1)) of MeJA for 3 days on plants. Under low temperature with the treatment under normal temperature as positive and that under low temperature on negative control. The growth indicators, relative electrolyte permeability, photosynthetic pigment content, antioxidant enzyme activity and hormone content of each treatment were measured, showing that the treatment with 10 μmol L~(–1) methyl jasmonate could reduce the damage of tobacco seedlings caused by low temperature. The effect of H_2O_2 was then verified by applications of DPI, 10 μmol L~(–1) MeJA, and DPI+MeJA with the same material and growth period, while low temperature treatment was used as a control. The contents of H_2O_2, O~(2–), CAT, MDA, and ASA-GSH cycle were determined. In conclusion, H_2O_2 in tobacco plants mainly exists as a poison molecule instead of a second messenger under the treatment of exogenous methyl jasmonate combined with low temperature.
Methyl jasmonate(MeJA) is an elicitor evolving in a variety of physiological and biochemical processes. To explore the effects of exogenous MeJA on tobacco seedlings under low temperature conditions, we used the tobacco variety "Yuyan 10" and sprayed four concentrations(1, 10, 100, 1000 μmol L~(–1)) of MeJA for 3 days on plants. Under low temperature with the treatment under normal temperature as positive and that under low temperature on negative control. The growth indicators, relative electrolyte permeability, photosynthetic pigment content, antioxidant enzyme activity and hormone content of each treatment were measured, showing that the treatment with 10 μmol L~(–1) methyl jasmonate could reduce the damage of tobacco seedlings caused by low temperature. The effect of H_2O_2 was then verified by applications of DPI, 10 μmol L~(–1) MeJA, and DPI+MeJA with the same material and growth period, while low temperature treatment was used as a control. The contents of H_2O_2, O~(2–), CAT, MDA, and ASA-GSH cycle were determined. In conclusion, H_2O_2 in tobacco plants mainly exists as a poison molecule instead of a second messenger under the treatment of exogenous methyl jasmonate combined with low temperature.
引文
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[10]Shin H,Min K,Arora R.Exogenous salicylic acid improves freezing tolerance of spinach(Spinacia oleracea L.)leaves.Cryobiology,2017,81:192-200.
[11]杨新良,陈也君,胡高云,李乾斌.NADPH氧化酶及其抑制剂的研究进展.药学学报,2016,51:499-506.Yang X L,Chen Y J,Hu G Y,Li Q B.Research progress of NADPH oxidases and their inhibitors.Acta Pharm Sin,2016,51:499-506(in Chinese with English abstract).
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[13]郑凤君,华南金秋,张立猛,李江舟,计思贵,林杉.长宽法测定幼苗期烟草叶面积的校正系数.中国烟草科学,2015,36(6):13-16.Zheng F J,Hua N J Q,Zhang L M,Li J Z,Ji S G,Lin S.Revision of the length-width method correction coefficient in measuring leaf area of tobacco plants at the seedling stage.Chin Tob Sci,2015,36(6):13-16(in Chinese with English abstract).
[14]K?hle H,Jeblick W,Poten F,Blaschek W,Kauss H.Chitosan-elicited callose synthesis in soybean cells as a ca-dependent process.Plant Physiol,1985,77:544-551.
[15]Lichtenthaler H K,Buschmann C.Chlorophylls and Carotenoids:Measurement and Characterization by UV-VIS Spectroscop.John Wiley&Sons,Inc,2001.p 1230.
[16]Veselov S Y,Kudoyarova G R,Egutkin N L,Gyuli-Zade V Z,Mustafina A R,Kof E M.Modified solvent partitioning scheme providing increased specificity and rapidity of immunoassay for indole-3-acetic acid.Physiol Plant,2010,86:93-96.
[17]Jabs T,Dietrich R A,Dangl J L.Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide.Science,1996,273:1853-1856.
[18]Dhindsa R S,Matowe W.Drought tolerance in two mosses:correlated with enzymatic defence against lipid peroxidation.JExp Bot,1981,32:79-91.
[19]Pinheiro H A,Damatta F M,Arm C,Fontes E P B,Loureiro M E.Drought tolerance in relation to protection against oxidative stress in clones of Coffea canephora subjected to long-term drought.Plant Sci,2004,167:1307-1314.
[20]Law M Y,Charles S A,Halliwell B.Glutathione and ascorbic acid in spinach(Spinacia oleracea)chloroplasts.The effect of hydrogen peroxide and of Paraquat.Biochem J,1983,210:899.
[21]Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.Plant Cell Physiol,1981,22:867-880.
[22]Foyer C H,Halliwell B.The presence of glutathione and glutathione reductase in chloroplasts:a proposed role in ascorbic acid metabolism.Planta,1976,133:21-25.
[23]Ghasemi P A,Sajjadi S E,Parang K.A review(research and patents)on jasmonic acid and its derivatives.Arch Pharm,2014,347:229-239.
[24]Battal P,Erez M E,Turker M,Berber I.Molecular and physiological changes in maize(Zea mays)induced by exogenous NAA,ABA and Me JA during cold stress.Ann Bot Fenn,2008,45:173-185.
[25]Fan L,Wang Q,Lyu J,Gao L,Zou J H,Shi J Y.Amelioration of postharvest chilling injury in cowpea(Vigna sinensis)by methyl jasmonate(MeJA)treatments.Sci Hortic(Amsterdam),2016,203:95-101.
[26]Cao S F,Zheng Y H,Wang K T,Rui H J,Tang S S.Effect of methyl jasmonate on cell wall modification of loquat fruit in relation to chilling injury after harvest.Food Chem,2010,118:641-647.
[27]Li D M,Guo Y K,Li Q,Zhang J,Wang X J,Bai J G.The pretreatment of cucumber with methyl jasmonate regulates antioxidant enzyme activities and protects chloroplast and mitochondrial ultrastructure in chilling-stressed leaves.Sci Hortic(Amsterdam),2012,143:135-143.
[28]Li X J,Yang M.Abscisic acid pretreatment enhances salt tolerance of rice seedlings:proteomic evidence.BBA-Proteins Proteom,2010,1804:929-940.
[29]De Smet I,Voss U,Lau S,Wilson M,Shao N,Timme RE,Swarup R,Kerr I,Hodqman C,Bock R,Bennett M,Jürqens G,Beeckman T.Unraveling the evolution of auxin signaling.Plant Physiol,2011,155:209-221.
[30]Mittler R.Oxidative stress,antioxidants and stress tolerance.Trends Plant Sci,2002,7:405-410.
[2]Augspurger C K.Reconstructing patterns of temperature,phenology,and frost damage over 124 years:spring damage risk is increasing.Ecology,2013,94:41-50.
[3]Menéndez A B,Rodriguez A A,Maiale S J,Rodriguez K M,Jimenez B J F,Ruiz O A.Polyamines Contribution to the Improvement of Crop Plants Tolerance to Abiotic Stress.Springer New York,2013,pp 113-136.
[4]Ma X,Chen C,Yang M,Dong X C,Lyu W,Meng Q W.Cold-regulated protein(Sl COR413IM1)confers chilling stress tolerance in tomato plants.Plant Physiol Biochem,2018,124:29-39.
[5]Per T S,Khan M I R,Anjum N A,Masood A,Hussain S J,Khan NA.Jasmonates in plants under abiotic stresses:crosstalk with other phytohormones matters.Environ Exp Bot,2018,145:104-120.
[6]Cai Y,Cao S,Yang Z,Zheng Y.MeJA regulates enzymes involved in ascorbic acid and glutathione metabolism and improves chilling tolerance in loquat fruit.Postharvest Biol Tech,2011,59:324-326.
[7]Qi X N,Xiao Y Y,Fan Z Q,Chen J Y,Lu W J,Kuang J F.A banana fruit transcriptional repressor MaERF10 interacts with Ma JAZ3 to strengthen the repression of JA biosynthetic genes involved in MeJA-mediated cold tolerance.Postharvest Biol Tech,2016,120:222-231.
[8]Wani S H,Kumar V,Shriram V,Sah S K.Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants.Crop J,2016,4:162-176.
[9]娄亚楠,王召军,杨欣玲,张洪映,牛德新,杨永锋,崔红.茉莉酸甲酯对烟草分泌型和非分泌型腺毛形态发生的影响.中国烟草学报,2018,24(2):24-29.Lou Y N,Wang Z J,Yang X L,Zhang H Y,Niu D X,Yang Y F,Cui H.Effects of methyl jasmonic acid on morphogenesis of tobacco glandular and non-glandular trichomes.Acta Tab Sin,2018,24(2):24-29(in Chinese with English abstract).
[10]Shin H,Min K,Arora R.Exogenous salicylic acid improves freezing tolerance of spinach(Spinacia oleracea L.)leaves.Cryobiology,2017,81:192-200.
[11]杨新良,陈也君,胡高云,李乾斌.NADPH氧化酶及其抑制剂的研究进展.药学学报,2016,51:499-506.Yang X L,Chen Y J,Hu G Y,Li Q B.Research progress of NADPH oxidases and their inhibitors.Acta Pharm Sin,2016,51:499-506(in Chinese with English abstract).
[12]韩锦峰,岳彩鹏,刘华山,苗红梅,王德勤,岳红波.烤烟生长发育的低温诱导研究:I.苗期低温诱导对烤烟顶芽发育及激素含量的影响.中国烟草学报,2002,8(1):27-31.Han J F,Yue C P,Liu H S,Miao H M,Wang D Q,Yue H B.Low temperature induction of growth and development of flue-cured tobacco:I.Effects of low temperature induction at seedling stage on shoot bud development and hormone content in flue-cured tobacco.Acta Tab Sin,2002,8(1):27-31(in Chinese with English abstract).
[13]郑凤君,华南金秋,张立猛,李江舟,计思贵,林杉.长宽法测定幼苗期烟草叶面积的校正系数.中国烟草科学,2015,36(6):13-16.Zheng F J,Hua N J Q,Zhang L M,Li J Z,Ji S G,Lin S.Revision of the length-width method correction coefficient in measuring leaf area of tobacco plants at the seedling stage.Chin Tob Sci,2015,36(6):13-16(in Chinese with English abstract).
[14]K?hle H,Jeblick W,Poten F,Blaschek W,Kauss H.Chitosan-elicited callose synthesis in soybean cells as a ca-dependent process.Plant Physiol,1985,77:544-551.
[15]Lichtenthaler H K,Buschmann C.Chlorophylls and Carotenoids:Measurement and Characterization by UV-VIS Spectroscop.John Wiley&Sons,Inc,2001.p 1230.
[16]Veselov S Y,Kudoyarova G R,Egutkin N L,Gyuli-Zade V Z,Mustafina A R,Kof E M.Modified solvent partitioning scheme providing increased specificity and rapidity of immunoassay for indole-3-acetic acid.Physiol Plant,2010,86:93-96.
[17]Jabs T,Dietrich R A,Dangl J L.Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide.Science,1996,273:1853-1856.
[18]Dhindsa R S,Matowe W.Drought tolerance in two mosses:correlated with enzymatic defence against lipid peroxidation.JExp Bot,1981,32:79-91.
[19]Pinheiro H A,Damatta F M,Arm C,Fontes E P B,Loureiro M E.Drought tolerance in relation to protection against oxidative stress in clones of Coffea canephora subjected to long-term drought.Plant Sci,2004,167:1307-1314.
[20]Law M Y,Charles S A,Halliwell B.Glutathione and ascorbic acid in spinach(Spinacia oleracea)chloroplasts.The effect of hydrogen peroxide and of Paraquat.Biochem J,1983,210:899.
[21]Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.Plant Cell Physiol,1981,22:867-880.
[22]Foyer C H,Halliwell B.The presence of glutathione and glutathione reductase in chloroplasts:a proposed role in ascorbic acid metabolism.Planta,1976,133:21-25.
[23]Ghasemi P A,Sajjadi S E,Parang K.A review(research and patents)on jasmonic acid and its derivatives.Arch Pharm,2014,347:229-239.
[24]Battal P,Erez M E,Turker M,Berber I.Molecular and physiological changes in maize(Zea mays)induced by exogenous NAA,ABA and Me JA during cold stress.Ann Bot Fenn,2008,45:173-185.
[25]Fan L,Wang Q,Lyu J,Gao L,Zou J H,Shi J Y.Amelioration of postharvest chilling injury in cowpea(Vigna sinensis)by methyl jasmonate(MeJA)treatments.Sci Hortic(Amsterdam),2016,203:95-101.
[26]Cao S F,Zheng Y H,Wang K T,Rui H J,Tang S S.Effect of methyl jasmonate on cell wall modification of loquat fruit in relation to chilling injury after harvest.Food Chem,2010,118:641-647.
[27]Li D M,Guo Y K,Li Q,Zhang J,Wang X J,Bai J G.The pretreatment of cucumber with methyl jasmonate regulates antioxidant enzyme activities and protects chloroplast and mitochondrial ultrastructure in chilling-stressed leaves.Sci Hortic(Amsterdam),2012,143:135-143.
[28]Li X J,Yang M.Abscisic acid pretreatment enhances salt tolerance of rice seedlings:proteomic evidence.BBA-Proteins Proteom,2010,1804:929-940.
[29]De Smet I,Voss U,Lau S,Wilson M,Shao N,Timme RE,Swarup R,Kerr I,Hodqman C,Bock R,Bennett M,Jürqens G,Beeckman T.Unraveling the evolution of auxin signaling.Plant Physiol,2011,155:209-221.
[30]Mittler R.Oxidative stress,antioxidants and stress tolerance.Trends Plant Sci,2002,7:405-410.