甘蔗根际土壤AM真菌群落季节性变化研究
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摘要
为了探明甘蔗根际土壤AM真菌群落季节性变化规律及其影响因素,2016年4月、8月、10月和12月在里建甘蔗试验基地,采集4个施肥水平(0%、25%、50%、100%)下的甘蔗根际土壤,采用高通量测序技术分析不同施肥水平下AM真菌群落季节变化规律。结果表明:4个季节共检测到10属AM真菌,AM真菌Chaol、Shannon-Wiener指数Shannon-Wiener和Invsimpsonindex指数从春季到冬季呈先降后升的趋势,夏季最低;4个季节共检测出281个AM真菌OTUs(operational taxonomic units),春季、夏季、秋季、冬季的OTUs数目分别为160、68、103、198个。4个季节甘蔗根际土壤中球囊霉属(Glomus)占有绝对的优势,之后是类球囊霉属(Paraglomus)和多样孢囊霉属(Diversispora)。此外,也有少量的原囊霉属(Archaeospora)、巨孢囊霉属(Gigaspora)、无梗囊霉属(Acaulospora)、盾巨孢囊霉属(Scutellospora)和两性球囊霉属(Ambispora)。不同季节AM真菌群落组成差异显著,但总体来说冬季和春季种群结构相似,夏季和秋季相似,而夏季和秋季的AM真菌种类比春季和冬季的AM真菌种类多。
In this paper, four seasonal variation of AM fungal community in the rhizosphere of sugarcane was investigated by Illumina MiSeq and influencing factors were analyzed. Soil samples were collected from the rhizosphere of sugarcane plants under four different fertilization levels(0%, 25%, 50% and 100%) in April, August, October and December 2016, respectively. A total of 10 genera of AM fungi were discovered. Shannon-Weiner indices, richness and Simpson Index of AM fungi decreased first and then increased from spring to winter, lowest values occurred in summer. A total of 281 AM fungal OTUs(operational taxonomic units) were detected in four seasons, with 160 OTUs, 68 OTUs, 103 OTUs, and 198 OTUs in spring, summer, autumn and winter, respectively. Glomus was the most dominant genus in the soils in the study, followed by Paraglomus and Diversispora. A small number of Archaeospora, Gigaspora, Acaulospora, Scutellospora and Ambispora species were also identified. AM fungal community compositions in summer and autumn were significantly different from that of spring and winter, while the difference was not significant between summer and autumn. AM fungi species in summer and autumn were more than those in spring and winter.
In this paper, four seasonal variation of AM fungal community in the rhizosphere of sugarcane was investigated by Illumina MiSeq and influencing factors were analyzed. Soil samples were collected from the rhizosphere of sugarcane plants under four different fertilization levels(0%, 25%, 50% and 100%) in April, August, October and December 2016, respectively. A total of 10 genera of AM fungi were discovered. Shannon-Weiner indices, richness and Simpson Index of AM fungi decreased first and then increased from spring to winter, lowest values occurred in summer. A total of 281 AM fungal OTUs(operational taxonomic units) were detected in four seasons, with 160 OTUs, 68 OTUs, 103 OTUs, and 198 OTUs in spring, summer, autumn and winter, respectively. Glomus was the most dominant genus in the soils in the study, followed by Paraglomus and Diversispora. A small number of Archaeospora, Gigaspora, Acaulospora, Scutellospora and Ambispora species were also identified. AM fungal community compositions in summer and autumn were significantly different from that of spring and winter, while the difference was not significant between summer and autumn. AM fungi species in summer and autumn were more than those in spring and winter.
引文
[1]孙吉庆,刘润进,李敏.丛枝菌根真菌提高植物抗逆性的效应及其机制研究进展[J].植物生理学报,2012, 48(9):845-852.
[2]Smith S E, Read D J. Mycorrhizal Symbiosis[M]. New York:Academic Press, 2010.
[3]DavisonJ,MooraM,?pikM,etal.Globalassessmentof arbuscularmycor-rhizalfungusdiversityrevealsverylow endemism[J]. Science, 2015, 349:970-973.
[4]vanderHeijdenMGA,MartinFM,SelosseMA,etal.Mycorrhizal ecology and evolution:the past, the present and the future[J]. New Phytologist, 2015, 205(4):1 406-1 423.
[5]刘欢.不同丛枝菌根真菌对四种植物生长特性影响[D].兰州:甘肃农业大学, 2016.
[6]Peng S L, Shen H, Guo T. Influence of arbuscular mycorrhizal fungi inoculation on 1-2 mm soil water stable aggregates in split root system[J]. Plant Nutrition and Fertilizer Science,2010, 16(6):1 546-1 550.
[7]Peng S L, Shen H, Guo T. Influence of mycorrhizal inoculationonwaterstableaggregatestraits[J].PlantNutritionand Fertilizer Science, 2010, 16(3):695-700.
[8]PengSL,YuanJJ,WeiCF,etal.Impactsofarbuscular mycorrhizalfungionsoilaggregationdynamicsofneutral purple soil[J]. Acta Ecologica Sinica, 2011, 31(2):498-505.
[9]黄京华,曾任森,骆世明.AM菌根真菌诱导对提高玉米纹枯病抗性的初步研究[J].中国生态农业学报,2006,14(3):167-169.
[10]黄京华,骆世明,曾任森.丛枝菌根菌诱导植物抗病的内在机制[J].应用生态学报, 2003, 14(5):819-822.
[11]伍荣冬,谭彩丽,李廷化,等.大田接种丛枝菌根真菌对甘蔗品种“福农41号”生长的影响[J].甘蔗糖业,2015(4):20-23.
[12]陈廷速,李松,王维赞,等.甘蔗大田接种AM菌剂效应研究[J].甘蔗糖业, 2013(3):6-10.
[13]张金莲,黄振瑞,车江旅,等. AM菌剂对大田甘蔗根际土壤AM真菌种群影响[J].西南农业学报,2015,28(1):269-273.
[14]陈廷速,甘立,李松,等.广西主要蔗区宿根蔗根际土壤微生物群落结构分析[J].亚热带农业研究, 2011, 7(2):129-131.
[15]王元贞,张木清,柯玉琴,等.菌根菌接种对甘蔗根系发育的影响[J].福建农业大学学报, 1995, 24(3):318-322.
[16]王元贞,张木清,柯玉琴,等.水分胁迫下菌根菌接种对蔗叶活性氧代谢的影响[J].中国生态农业学报, 1995, 3(2):10-15.
[17]陈廷速,李松,张金莲,等.丛枝菌根(AM)真菌对甘蔗根系侵染研究[J].西南农业学报, 2011, 24(5):1 757-1 760.
[18]林先贵,郝文英.不同植物对VA菌根的依性[J].植物学报, 1989, 31(9):721-725.
[19]刘敏,峥嵘,白淑兰,等.内蒙古大青山灌木铁线莲根围丛枝菌根真菌群落季节动态研究[J].西北植物学报,2016, 36(9):1 891-1 899.
[20]Zangaro W, Rostirola L V, de Souza P B, et al. Root colonization and spore abundance of arbuscular mycorrhizal fungi indistinctsuccessionalstagesfromanAtlanticrainforest biomeinsouthernBrazil[J].Mycorrhiza,2013,23(3):221-233.
[21]Hewins C R, Carrino-Kyker S R, Burke D J. Seasonal variationinmycorrhizalfungicolonizingrootsofAlliumtricoccum(wildleek)inamaturemixedhardwoodforest[J].Mycorrhiza, 2015, 25(6):469-483.
[22]HazardC,BootsB,KeithAM,etal.Temporalvariation outweighs effects of biosolids applications in shaping arbuscularmycorrhizalfungicommunitiesonplantsgrownin pastureandarablesoils[J].AppliedSoilEcology,2014,82(7):52-60.
[23]Bainard L D, Bainard J D, Hamel C, et al. Spatial and temporalstructuringofarbuscularmycorrhizalcommunitiesis differentially influenced by abiotic factors and host crop in a semi-aridprairieagroecosystem[J].FEMSMicrobiology Ecology, 2014, 88(2):333-344.
[24]峥嵘,白淑兰,李龙,等.不同季节土壤绣线菊根围丛枝菌根真菌群落差异性研究[J].干旱区研究,2017,34(5):1 049-1 055.
[25]贺学礼,耿晓进,赵丽莉,等.金银花根围AM真菌多样性的季节性变化研究[J].生态环境学报,2013,22(1):90-94.
[26]高萍,闫飞扬,蒙程,等.黄土高原不同耕作措施下AM真菌的多样性[J].草业科学,2016,33(10):1917-1 923.
[27]JansaJ,MozafarA,AnkenT,etal.Diversityandstructure ofAMFcommunitiesasaffectedbytillageinatemperate soil[J]. Mycorrhiza, 2002, 12(5):225-234.
[2]Smith S E, Read D J. Mycorrhizal Symbiosis[M]. New York:Academic Press, 2010.
[3]DavisonJ,MooraM,?pikM,etal.Globalassessmentof arbuscularmycor-rhizalfungusdiversityrevealsverylow endemism[J]. Science, 2015, 349:970-973.
[4]vanderHeijdenMGA,MartinFM,SelosseMA,etal.Mycorrhizal ecology and evolution:the past, the present and the future[J]. New Phytologist, 2015, 205(4):1 406-1 423.
[5]刘欢.不同丛枝菌根真菌对四种植物生长特性影响[D].兰州:甘肃农业大学, 2016.
[6]Peng S L, Shen H, Guo T. Influence of arbuscular mycorrhizal fungi inoculation on 1-2 mm soil water stable aggregates in split root system[J]. Plant Nutrition and Fertilizer Science,2010, 16(6):1 546-1 550.
[7]Peng S L, Shen H, Guo T. Influence of mycorrhizal inoculationonwaterstableaggregatestraits[J].PlantNutritionand Fertilizer Science, 2010, 16(3):695-700.
[8]PengSL,YuanJJ,WeiCF,etal.Impactsofarbuscular mycorrhizalfungionsoilaggregationdynamicsofneutral purple soil[J]. Acta Ecologica Sinica, 2011, 31(2):498-505.
[9]黄京华,曾任森,骆世明.AM菌根真菌诱导对提高玉米纹枯病抗性的初步研究[J].中国生态农业学报,2006,14(3):167-169.
[10]黄京华,骆世明,曾任森.丛枝菌根菌诱导植物抗病的内在机制[J].应用生态学报, 2003, 14(5):819-822.
[11]伍荣冬,谭彩丽,李廷化,等.大田接种丛枝菌根真菌对甘蔗品种“福农41号”生长的影响[J].甘蔗糖业,2015(4):20-23.
[12]陈廷速,李松,王维赞,等.甘蔗大田接种AM菌剂效应研究[J].甘蔗糖业, 2013(3):6-10.
[13]张金莲,黄振瑞,车江旅,等. AM菌剂对大田甘蔗根际土壤AM真菌种群影响[J].西南农业学报,2015,28(1):269-273.
[14]陈廷速,甘立,李松,等.广西主要蔗区宿根蔗根际土壤微生物群落结构分析[J].亚热带农业研究, 2011, 7(2):129-131.
[15]王元贞,张木清,柯玉琴,等.菌根菌接种对甘蔗根系发育的影响[J].福建农业大学学报, 1995, 24(3):318-322.
[16]王元贞,张木清,柯玉琴,等.水分胁迫下菌根菌接种对蔗叶活性氧代谢的影响[J].中国生态农业学报, 1995, 3(2):10-15.
[17]陈廷速,李松,张金莲,等.丛枝菌根(AM)真菌对甘蔗根系侵染研究[J].西南农业学报, 2011, 24(5):1 757-1 760.
[18]林先贵,郝文英.不同植物对VA菌根的依性[J].植物学报, 1989, 31(9):721-725.
[19]刘敏,峥嵘,白淑兰,等.内蒙古大青山灌木铁线莲根围丛枝菌根真菌群落季节动态研究[J].西北植物学报,2016, 36(9):1 891-1 899.
[20]Zangaro W, Rostirola L V, de Souza P B, et al. Root colonization and spore abundance of arbuscular mycorrhizal fungi indistinctsuccessionalstagesfromanAtlanticrainforest biomeinsouthernBrazil[J].Mycorrhiza,2013,23(3):221-233.
[21]Hewins C R, Carrino-Kyker S R, Burke D J. Seasonal variationinmycorrhizalfungicolonizingrootsofAlliumtricoccum(wildleek)inamaturemixedhardwoodforest[J].Mycorrhiza, 2015, 25(6):469-483.
[22]HazardC,BootsB,KeithAM,etal.Temporalvariation outweighs effects of biosolids applications in shaping arbuscularmycorrhizalfungicommunitiesonplantsgrownin pastureandarablesoils[J].AppliedSoilEcology,2014,82(7):52-60.
[23]Bainard L D, Bainard J D, Hamel C, et al. Spatial and temporalstructuringofarbuscularmycorrhizalcommunitiesis differentially influenced by abiotic factors and host crop in a semi-aridprairieagroecosystem[J].FEMSMicrobiology Ecology, 2014, 88(2):333-344.
[24]峥嵘,白淑兰,李龙,等.不同季节土壤绣线菊根围丛枝菌根真菌群落差异性研究[J].干旱区研究,2017,34(5):1 049-1 055.
[25]贺学礼,耿晓进,赵丽莉,等.金银花根围AM真菌多样性的季节性变化研究[J].生态环境学报,2013,22(1):90-94.
[26]高萍,闫飞扬,蒙程,等.黄土高原不同耕作措施下AM真菌的多样性[J].草业科学,2016,33(10):1917-1 923.
[27]JansaJ,MozafarA,AnkenT,etal.Diversityandstructure ofAMFcommunitiesasaffectedbytillageinatemperate soil[J]. Mycorrhiza, 2002, 12(5):225-234.
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