重金属铅对实验红鲫生理生化指标影响的研究
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摘要
为了检测重金属铅离子对实验红鲫的生物毒性效应,以实验红鲫为受试鱼类,观察实验红鲫在不同浓度重金属铅溶液中的生长情况、死亡率、异常状态以及血红细胞微核和核异常情况,并检测血液中乳酸脱氢酶(lactate dehydrogenase,LDH)的变化情况指标,分析不同浓度的铅对实验红鲫生理状态的影响。结果发现与空白对照组相比,实验红鲫在安全浓度范围内未出现死亡,生长状态良好,随着重金属铅浓度的不断增加,核异常率明显发生变化,血液中的乳酸脱氢酶也有明显的变化。
In order to detect the biological toxicity of heavy metal lead,the red crucian carp was used as the tested fish,and the mortality,growth,abnormal state of the red crucian carp in the lead solution were observed,and the changes of the micronucleus and nuclear abnormalities and the lactate dehydrogenase( ldh) in the blood of the red crucian carp were detected. Then it analyzed the physiological and piochemical effects of red crucian carp in the different concentrations of lead nitrate. Then it found the red crucian carp did not die in the safe concentration range,and the growth state was good,but the rate of nuclear abnormality was obviously changed with the increasing of heavy metal lead concentration and ldh in the blood also had significant changes compared with the control group.
In order to detect the biological toxicity of heavy metal lead,the red crucian carp was used as the tested fish,and the mortality,growth,abnormal state of the red crucian carp in the lead solution were observed,and the changes of the micronucleus and nuclear abnormalities and the lactate dehydrogenase( ldh) in the blood of the red crucian carp were detected. Then it analyzed the physiological and piochemical effects of red crucian carp in the different concentrations of lead nitrate. Then it found the red crucian carp did not die in the safe concentration range,and the growth state was good,but the rate of nuclear abnormality was obviously changed with the increasing of heavy metal lead concentration and ldh in the blood also had significant changes compared with the control group.
引文
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[2]兰砥中.湖南某典型铅锌矿区农业土壤及农作物中重金属的风险评价[D].长沙:湖南农业大学,2014.
[3]杨霞,彭渤,吴雅霁,等.湘江湘潭段河岸沉积物重金属污染地球化学分析[J].地球化学,2016,45(1):62-65.
[4]吴端生.实验红鲫标准化研究与应用的现状及展望[J].实验动物科学,2016,33(3):56-60.
[5]EL FEKY M G,EL MOWAFY A A,WARITH A A,et al.Geochemistry,radioactivity and environmental impacts of Gabal Marwa granites,southeastern Sinai,Egypt[J].Chinese Journal of Geochemistry,2011,30(2):175-186.
[6]豆长明,周鸣鸣,张洁,等.铅胁迫对斑马鱼抗氧化酶活性的影响[J].环境科技,2012,25(5):1-4.
[7]何理平,曹彩辉,吴端生.镉离子对红鲫鱼血红细胞微核和核异常的影响研究[J].实验动物科学,2012,29(5):30-34.
[8]陈国柱,方展强.铜、锌、镉对唐鱼胚胎及初孵仔鱼的急性毒性及安全浓度评价[J].生物学杂志,2011,28(2):28-31.
[9]FU J,HU X,TAO X C,et al.Risk and toxicity assessments of heavy metals in sediments and fishes from the Yangtze River and Taihu Lake,China[J].Chemosphere,2013,93(9):1887-1895.
[10]KE X,BAO Q H,QI Y F,et al.Toxicity assessment of sediments from the Liaohe River Protected Area(China)under the influence of ammonia nitrogen,heavy metals and organic contaminants[J].Environmental toxicology and pharmacology,2018,59(2):34-42.
[11]张彩明,陈应华.重金属胁迫鱼类毒性试验研究进展[J].中国渔业经济,2012,30(4):150-155.