基于GC-QTOF-MS代谢组学技术研究饲喂不同糖饲料对蜜蜂所产蜂王浆中代谢物组成成分的影响
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摘要
为研究饲喂不同糖饲料对蜜蜂所产蜂王浆品质的影响,基于气相色谱-四极杆飞行时间质谱代谢组学技术对3种不同糖饲料饲喂条件下(白糖、蜂蜜、高果糖浆)蜜蜂所生产的蜂王浆进行分析。结果表明:与白糖组相比,蜂蜜组脯氨酸、异亮氨酸、缬氨酸、乳糖、癸酸、L-磺基丙氨酸等含量显著较高(P<0.05);与蜂蜜组相比较,高果糖浆组蔗糖、麦芽糖含量显著较高(P<0.05),而甘油、纤维二糖、异亮氨酸、木糖、羟脯氨酸、乳糖、L-磺基丙氨酸含量显著较低(P<0.05);高果糖浆组与白糖组比较,软脂酸、麦芽糖、苏氨酸、半乳糖含量显著较高(P<0.05),而纤维二糖、松二糖、木糖、乳糖、赤藓糖含量显著较低(P<0.05)。这种由于不同糖饲料饲喂条件而导致的差异可能会对蜜蜂及蜜蜂幼虫生长发育以及蜂王浆品质产生一定的影响,同时为蜂农饲喂方式的选择以及进一步研究影响蜂王浆组成差异的因素提供理论依据。
This study aimed to examine the effects of different dietary sugars for honeybees on the quality of royal jelly.Metabonomics analysis of royal jelly produced by honeybees fed three different sugars, white sugar, honey and high fructose syrup was conducted using gas chromatography-quadrupole time-of-flight mass spectrometry(GC-QTOF-MS).The results showed that the contents of proline, isoleucine, valine, lactose, hydroxyproline, capric acid and L-sulfanalanine were significantly higher in the honey-fed group compared with the sugar-fed group(P < 0.05). The contents of sucrose and maltose were significantly higher(P < 0.05), whereas the contents of glycerol, cellobiose, isoleucine, xylose, hydroxyproline,lactose and L-sulfaalanine were significantly lower in high fructose syrup group compared with the honey-fed group(P < 0.05). The contents of palmitic acid, maltose, threonine and galactose were significantly higher in the high fructose syrup-fed group(P < 0.05), while the contents of cellobiose, pine disaccharides, xylose, lactose and erythritol were significantly lower in the high fructose syrup-fed group compared with the sugar-fed group(P < 0.05). These differences may affect the growth and development of honeybee larvae and also have an impact on royal jelly quality. The results of this study provide a theoretical basis for the selection of appropriate dietary sugars for honeybees and for further research on the factors that affect the composition of royal jelly.
This study aimed to examine the effects of different dietary sugars for honeybees on the quality of royal jelly.Metabonomics analysis of royal jelly produced by honeybees fed three different sugars, white sugar, honey and high fructose syrup was conducted using gas chromatography-quadrupole time-of-flight mass spectrometry(GC-QTOF-MS).The results showed that the contents of proline, isoleucine, valine, lactose, hydroxyproline, capric acid and L-sulfanalanine were significantly higher in the honey-fed group compared with the sugar-fed group(P < 0.05). The contents of sucrose and maltose were significantly higher(P < 0.05), whereas the contents of glycerol, cellobiose, isoleucine, xylose, hydroxyproline,lactose and L-sulfaalanine were significantly lower in high fructose syrup group compared with the honey-fed group(P < 0.05). The contents of palmitic acid, maltose, threonine and galactose were significantly higher in the high fructose syrup-fed group(P < 0.05), while the contents of cellobiose, pine disaccharides, xylose, lactose and erythritol were significantly lower in the high fructose syrup-fed group compared with the sugar-fed group(P < 0.05). These differences may affect the growth and development of honeybee larvae and also have an impact on royal jelly quality. The results of this study provide a theoretical basis for the selection of appropriate dietary sugars for honeybees and for further research on the factors that affect the composition of royal jelly.
引文
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[2]STEINER H,HULTMAPK D,ENGSTRM A,et al.Sequence and specificity of two antibacterial proteins involved in insect immunity[J].Nature,1981,292:246-248.
[3]FEHLBAUM P,BULET P,CHERNYSH S,et al.Structure-activity analysis of thanatin,a 21-residue inducible insect de-fense peptide with sequence homology to frog skin antimicrobial peptides[J].Proceedings of the National Academy of Sciences,1996,93(3):1221-1225.DOI:10.1073/pnas.93.3.1221.
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[10]李肖.饲料蛋白源对意大利蜜蜂繁蜂效果及蜂王浆品质的影响[D].泰安:山东农业大学,2014.
[11]程艳华.蛋白质饲料对工蜂初生重与咽下腺及产浆的影响[D].福州:福建农林大学,2008.
[12]王改英,康明江,杨维仁,等.蜜蜂人工饲料对蜂王浆产量及品质的影响[J].蜜蜂杂志,2010,30(2):6-8.DOI:10.3969/j.issn.1003-9139.2010.02.029.
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[22]董文滨,胥保华.蜜蜂幼虫发育所需营养物质[J].中国蜂业,2013(7):12-14.
[23]廖国周,柴仕名.支链氨基酸营养研究进展[J].饲料博览,2005(4):7-10.DOI:10.3969/j.issn.1001-0084.2005.04.003.
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[27]吴雨祺,蔺哲广,郑火青,等.蜜蜂上颚腺及其分泌物研究进展[J].昆虫学报,2015(8):911-918.DOI:10.16380/j.kcxb.2015.08.012.
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[30]BOGDANOV S.Royal jelly,bee brood:composition,health,medicine:a review[J].Bee Product Science,2016,4:1-36.
[31]余林生.碳水化合物在蜜蜂体内的代谢[J].养蜂科技,1996(4):7-9.
[32]侯翠翠.碳水化合物在蜜蜂体内的代谢[J].科技创新导报,2012(4):224-225.DOI:10.3969/j.issn.1674-098X.2012.04.189.
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