柯里拉京与DNA相互作用的光谱研究
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摘要
在p H 7.4的生理条件下,以溴化乙锭(EB)作为荧光探针,利用荧光光谱、紫外-可见吸收光谱、共振散射光谱法结合盐效应和DNA熔点(T_m)实验研究了柯里拉京(Cor)与小牛胸腺DNA分子之间的相互作用机制。实验结果表明,Cor静态猝灭DNA-EB体系的荧光。Cor与DNA作用后,其特征吸收峰强度发生减色效应;与DNA作用导致Cor在480.5 nm处的共振散射峰增强,并在330.2 nm处出现新共振散射峰。盐效应对Cor与DNA分子相互作用的影响较小。与Cor作用引起DNA的T_m值升高5.5℃。由此推断,Cor与DNA相互作用的主要方式为嵌插,两者间形成了超分子体系。通过计算获得Cor与DNA间结合常数(K_A)为5.82×10~3L/mol(298 K)、2.47×10~4L/mol(310 K),它们之间的作用为熵驱动的自发、吸热过程,疏水作用力是主要的非共价作用方式。
Under the physiological condition of p H 7.4,using ethidium bromide( EB) as the fluorescent probe,the interaction mechanism between coriolis( Cor) and calf thymus DNA was studied by UV-Vis absorption spectroscopy,resonance scattering spectroscopy combined with salt effect and DNA melting point. Cor can quench the fluorescence of EB-DNA system through a static process.The subtractive effect of the characteristic absorption peak occurrs after Cor interacts with DNA. The interaction leads to the enhancement of Cor resonance at 481 nm and a new resonance scattering peak at 330 nm. The salt effect has a little influence on the interaction between Cor and DNA. The interaction leads to the increase of the T_m of DNA by 5.5 ℃. It is deduced that the main way of the interaction between Cor and DNA is intercalation,and a supramolecular system is formed between them. The binding constant( K_A) between Cor and DNA is 5.82×10~3 L/mol( 298 K) and 2. 47 ×10~4 L/mol( 310 K). The interaction between Cor and DNA is entropy-driven spontaneous,endothermic. Hydrophobic forces are the main non-covalent mode of action.
Under the physiological condition of p H 7.4,using ethidium bromide( EB) as the fluorescent probe,the interaction mechanism between coriolis( Cor) and calf thymus DNA was studied by UV-Vis absorption spectroscopy,resonance scattering spectroscopy combined with salt effect and DNA melting point. Cor can quench the fluorescence of EB-DNA system through a static process.The subtractive effect of the characteristic absorption peak occurrs after Cor interacts with DNA. The interaction leads to the enhancement of Cor resonance at 481 nm and a new resonance scattering peak at 330 nm. The salt effect has a little influence on the interaction between Cor and DNA. The interaction leads to the increase of the T_m of DNA by 5.5 ℃. It is deduced that the main way of the interaction between Cor and DNA is intercalation,and a supramolecular system is formed between them. The binding constant( K_A) between Cor and DNA is 5.82×10~3 L/mol( 298 K) and 2. 47 ×10~4 L/mol( 310 K). The interaction between Cor and DNA is entropy-driven spontaneous,endothermic. Hydrophobic forces are the main non-covalent mode of action.
引文
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[2]KAKIUCHI N,HATTORI M,NAMBA T,et al..Inhibitory effect of tannins on reverse transcriptase from RNA tumor virus[J].J.Nat.Prod.,1985,48(4):614-621.
[3]HAU D K,GAMBARI R,WONG R S,et al..Phyllanthus urinaria extract attenuates acetaminophen induced hepatotoxicity:involvement of cytochrome P450 CYP2E1[J].Phytomedicine,2009,16(8):751-760.
[4]PELLATI F,BRUNI R,RIGHI D,et al..Metabolite profiling of polyphenols in a Terminalia chebula Retzius ayurvedic decoction and evaluation of its chemopreventive activity[J].J.Ethnopharmacol.,2013,147(2):277-285.
[5]陈一燕,陈崇宏.柯里拉京药理活性研究进展[J].中国现代应用药学,2010(5):390-394.CHEN Y Y,CHEN C H.Advances in the pharmacological activity research on corilagin[J].Chin.JMAP,2010(5):390-394.(in Chinese)
[6]MING Y L,ZHENG Z Z,CHEN L H,et al..Corilagin inhibits hepatocellular carcinoma cell proliferation by inducing G2/M phase arrest[J].Cell Biol.Int.,2013,37(10):1046-1054.
[7]JIA L,JIN H,ZHOU J,et al..A potential anti-tumor herbal medicine,corilagin,inhibits ovarian cancer cell growth through blocking the TGF-p signaling pathways[J].BMC Complement.Altern.Med.,2013,13(1):1-11.
[8]ZHANG Y J,NAGAO T,TANAKA T,et al..Antiproliferative activity of the main constituents from phyllauthus emblica[J].Biol.Pharm.Bull.,2004,27(2):251-255.
[9]李小芳,冯小强,杨声,等.丁二酸、1,10-菲啰啉构筑稀土配合物的合成、表征及与DNA作用的光谱学研究[J].发光学报,2016,37(5):617-623.LI X F,FENG X Q,YANG S,et al..Synthesis,characterization of rare earth complexes with succinic acid and 1,10-phenanthroline and interacting with DNA[J].Chin.J.Lumin.,2016,37(5):617-623.(in Chinese)
[10]WANG B Q.Corilagin nanoparticle-induced apoptosis in human gastric cancer SGC-7901 cells via the mitochondrial pathway[J].Acta Pharmacol.Sinica,2013,34:14-14.
[11]GAMBARI R,HAU D K,WONG W Y,et al..Sensitization of Hep3B hepatoma cells to cisplatin and doxorubicin by corilagin[J].Phytother.Res.,2013.28(51):781-783.
[12]黄玉玲,付彩霞,刘茂钘.叶酸与鲱鱼精DNA作用机制的光谱研究[J].化学研究与应用,2017,29(1):8-12.HUANG Y L,FU C X,LIU M X.The interaction mechanism study between folic acid and herring sperm DNA by spectroscopic method[J].Chem.Res.App.,2017,29(1):8-12.(in Chinese)
[13]李小芳,冯小强,杨声.8-羟基喹啉-山梨酸稀土配合物与鲱鱼精DNA相互作用的光谱研究[J].发光学报,2017,38(3):387-394.LI X F,FENG X Q,YANG S.DNA binding of rare earth complexes containing mixed ligands of sorbic acid and 8-hydroxyquinoline by spectroscopy[J].Chi.J.Lumin.,2017,38(3):387-394.(in Chinese)
[14]YU F S,DING Y B,GAO Y W.Fluorescence enhancement effect for the determ ination of DNA with calcein-cetyltrimethyl ammonium brom ide system[J].Analyt.Chim.Acta,2008,625(2):195-200.
[15]余玉叶,俞世钢,赵国良.4-甲基-1,2,3-噻二唑-5-甲酸钕配合物的合成、晶体结构及与DNA作用[J].中国稀土学报,2015,22(6):697-704.YU Y Y,YU S G,ZHAO G L.Synthesis,crystal structure and DNA binding of neodymium complex with 4-methyl-1,2,3-thiadiazol-5-arboxylic acid[J].J.Chin.Soc.Rare Earth.,2015,22(6):697-704.(in Chinese)
[16]WANG W,ZHANG W,DUAN Y K,et al..Investigation of the binding sites and orientation of caffeine on human serum albumin by surface-enhanced Raman scattering and molecular docking[J].Spectrochim.Acta A:Mol.Biomol.Spectrosc.,2013,115(11):57-63.
[17]张勇,张贵珠,王月梅.光谱法研究丝裂霉素、血清白蛋白以及金属离子间的相互作用[J].分析科学学报,2000,16(6):445-448.ZHANG Y,ZHANG G Z,WANG Y M.Studies of interaction of mitomycin C and serum albumin by spectrum method[J].J.Ana.Sci.,2000,16(6):445-448.(in Chinese)
[18]TANFORD C.The Hydrophobic Effect:Formation of Micciies and Biological Membranes[M].New york:John Wiley and Sons,1973.
[19]饶震红,刘世禄,孙英,等.共振光散射法研究甲萘威与DNA的作用及其应用[J].光谱学与光谱分析,2008,28(2):399-402.RAO Z H,LIU S L,SUN Y,et al..Study on the interaction of carbaryl and ct DNA by resonance light scattering spectra and its application[J].Spectrosc.Spect.Anal.,2008,28(2):399-402.(in Chinese)
[20]LEE J H,KIM H L,LEE M H,et al..Asiaticoside rnhances normal human skin cell migration,attachment and growth in vitro wound healing,model[J].Phytomedicine,2012,19(13):1223-1227.